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Citations for Bio-Rad :
7801 - 7850 of 7899 citations for 6 Quinoxalinecarboxaldehyde 1 2 3 4 tetrahydro 1 methyl 3 oxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were resuspended in MACS buffer (PBS +2% FBS +2mM EDTA) and counted using trypan blue with the TC20™ Automated Cell Counter (Bio-Rad). In some cases ...
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bioRxiv - Neuroscience 2019Quote: ... The PCR product was confirmed by using 2% agarose gel electrophoresis and visualized under a Gel Doc system (BioRad, Hercules, CA, USA). The amplified products were purified with the QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... which was performed using a 2 μl cDNA /20 μl reaction volume on a CFX Connect™ real-time PCR detection system (Bio-Rad) using SYBR Green according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... The mixed beads were washed once with the washing buffer and biotinylated proteins were eluted by 30 μL 2× SDS sample buffer and separated by SDS-PAGE (Biorad, Hercules, CA).
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bioRxiv - Microbiology 2020Quote: Serum samples were inactivated with γ-irradiation (2 mRad) and cytokine concentrations were determined on a Bio-Plex 200 instrument (Bio-Rad) using Milliplex Mouse Cytokine/Chemokine MAGNETIC BEAD Premixed 25 Plex Kit (Millipore) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 10 μL of reconstituted representative influenza vaccine (i.e., AddaVax/Y-2) of Fluad Quadrivalent was mixed with Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and β-mercaptoethanol (2% ...
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bioRxiv - Microbiology 2019Quote: ... and FLAG-cMCF-HA recovered as described above were prepped using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad 1632130). The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG ...
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bioRxiv - Neuroscience 2020Quote: ... brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots of 50 μg protein in SDS-PAGE sample buffer containing 0.2 M dithiothreitol were heat-denatured for 5 min at 95 °C then electrophoresed on AnyKD midi criterion™ gels (Bio-Rad) in ProSieve™ EX running buffer (Lonza) ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad, Veenendaal, The Netherlands).
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were detected by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat anti-mouse IgG (BioRad ...
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bioRxiv - Microbiology 2022Quote: We finalized the IPATS-BLV reaction in a 22-μl reaction mixture containing 14 μl of 2× ddPCR Supermix for Probes (Bio-Rad, #1863023), 909 nM of primers except the RPP30 primers (DRB3*016:01-forward ...
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bioRxiv - Immunology 2022Quote: ... To each well 50 µL of 2 µg/mL of biotin-conjugated mouse anti-bovine IFN-γ mAb (CC302, Bio-Rad Antibodies) diluted in PBS was added and plates incubated for 2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
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bioRxiv - Biophysics 2022Quote: ... Samples were centrifuged for 2 min at 12,000 x g before being separated on 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) at 160 V for 60 min ...
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bioRxiv - Biophysics 2024Quote: ... and incubated for 3 hours at 37 °C and 5% CO2 with GPMV-vesiculation buffer consisting of 25 mM methanol-free formaldehyde (FisherScientific, cat# 11586711) and 2 mM dithiothreitol (Biorad, cat# 1610610) dissolved in HBSS ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted four-fold and 2 µL was used for quantitative PCR (qPCR) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725274) and 500 nM of gene-specific qPCR primers (see Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm nitrocellulose membrane for proteins larger than 35 kDa and to 0.1 µm nitrocellulose or 0.2 µm PVDF membranes for proteins smaller than 35 kDa for 30 min using Trans-Blot Turbo transfer system (Bio-Rad, 1704150) or for 2.5 h using wet transfer system (TG buffer ...
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bioRxiv - Microbiology 2024Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using the gDNA of purified viruses was performed in parallel with each experiment ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Immunology 2023Quote: The detection of IgG ANAs in the serum of Ngfr KO and Bcl2 Tg mice was performed by IF in Hep-2 coated slides (Kallestad™ Bio-Rad) following manufacturer instructions ...
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bioRxiv - Genetics 2023Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd.) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd.) ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were added to a 2 mL bed of Ni-NTA agarose resin in a 20 mL column (Bio-Rad 7321010) equilibrated with wash buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Microbiology 2023Quote: ... The horse-radish peroxidase conjugated to the secondary antibody was detected by incubating the membrane with 2 mL chemiluminescence substrate (Bio-Rad, 1705062) for five minutes and then imaged using a ChemiDoc XRS+ imager (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Concentration of the RNA was determined by a NanoDrop UV spectrophotometer and 2 μg of RNA was loaded onto 15% Criterion-TBE-Urea polyacrylamide gel (Biorad, cat#3450093) for size selection ...
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bioRxiv - Evolutionary Biology 2023Quote: Protein was separated by electrophoresis on 12% SDSCPAGE and transferred to a PVDF (polyvinylidene difluoride) membrane at 100 mA for 2 h using a Mini-PROTEAN Tetra system (Bio-Rad, USA). For detection of PpSerpin1 isoform proteins or PrPAP1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated products were electrophoresed through at 2% agarose gel stained with ethidium bromide and imaged on the GelDoc imager and camera system (BioRad, Hercules, CA).
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bioRxiv - Microbiology 2023Quote: ... Droplet Digital PCR (ddPCR) was performed to measure HSV-2 genomic DNA levels in DRGs using the QX100 droplet digital PCR system (Bio-Rad Laboratories). HSV-2 genomic DNA was quantified using the following gD primers/TaqMan probe set ...
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bioRxiv - Molecular Biology 2023Quote: ... fractions with Afl RT were pooled and loaded onto a 2-ml Macro-Prep DEAE Resin (Bio-Rad, column CA, Hercules, USA) pre-equilibrated with a buffer C (50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... using cDNA generated from 0.1-2 ng total RNA with the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad catalog # 1864021) according to the manufacturer’s protocol with reverse transcription at 42°C and annealing/extension at 60°C.
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bioRxiv - Biochemistry 2024Quote: Purified polar flagellins (2 µg) were separated by 12% SDS-PAGE as described previously [33] and stained with Coomassie Blue (BioRad, Madrid, Spain), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Molecular Biology 2024Quote: ... The relative number of cells in each well was evaluated after solubilization in 40 µL of 2% Triton X-100 by measuring absorbance at 595 nm in a microplate reader (Bio-Rad Laboratories). All determinations were carried out in triplicate.
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bioRxiv - Microbiology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 ng of cDNA were used in each qPCR reaction on a CFX Connect Real-time PCR detection system (Bio-Rad, Hercules, CA) with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8 μm cryostat sections of C51 and F9 tumors stained with IgG (L19)-FITC (final concentration 2 μg/mL) and detected with a Rabbit anti-FITC (Bio-Rad; 4510-7804) and Donkey anti-Rabbit AlexaFluor 488 (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The blood was first separated from the ethanol through gentle centrifugation and embedded in molten 2% agarose plugs (Bio-Rad DNA plug kit). The solidified plugs were submerged in Lysis Buffer solution (Bionano ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 hours at room temperature with HRP-conjugated rabbit secondary antibody (Bio-Rad, USA, 170-6515; RRID: AB_11125142) for NALCN detection and HRP-conjugated mouse secondary antibody (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are listed in Supplemental table 2 Immunoreactive proteins were visualized with Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA, USA). The UVP ChemStudio (Analytik Jena US LLC ...
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bioRxiv - Cell Biology 2021Quote: ... were amplified using Phusion High-Fidelity PCR MasterMix (primer sequences are listed in Additional File 2) and a C1000 thermocycler (Bio-Rad Life Science). The peak sequences were amplified for 98 °C for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with specific primers (Supplementary Table 2) using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) and CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... and Reverse Transcription (RT) was performed with 2 µg RNA per 40 µl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
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bioRxiv - Biophysics 2022Quote: ... the samples were cooled on ice for 10 min followed by the addition of 80 mg Bio-beads SM-2 Resin (Bio-Rad, Hercules, CA), then rotated at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... ddPCR was used to detect DNA of SP by using a QX100 Droplet Digital PCR System and 2× ddPCR Supermix (Bio-Rad, California, CA) with protocols developed in our previous studies 48-57 ...
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bioRxiv - Cell Biology 2022Quote: ... and Reverse Transcription (RT) was performed with 2 μg RNA per 40 μl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
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bioRxiv - Genetics 2022Quote: ... a small implant of the thallus of interest (0.5 cm2) was set in 2 mL tube containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Pathology 2019Quote: ... this amount of protein was broken down via a precipitation and purification kit (ReadyPrep™ 2-D Cleanup Kit, Bio-Rad, Warsaw, Poland). The obtained protein pellets were then dissolved in a rehydration buffer ...
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bioRxiv - Immunology 2020Quote: ... The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories, USA) with 2 mM ATP (MP Biomedicals ...