Labshake search
Citations for Bio-Rad :
7651 - 7700 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cDNA was made from equivalent starting amounts for each sample and synthesized using the iScript cDNA Synthesis Kit (Bio-Rad). Total cDNA was diluted 1:10 in water and amplified by real-time polymerase chain reaction using a BioRad CFX-96 iCycler and SYBER Green Supermix ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA was reverse transcribed to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Biorad, Hercules, California) in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... and NuPAGE sample buffer and reducing buffer (LifeTechnologies) were added after measuring and adjusting the samples for protein concentration (DC protein assay kit II, Bio-Rad). 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted from 40 w1118 or de2f1b 80-85 hr AEL salivary glands using the Aurum Total RNA Mini Kit (Bio-Rad) with treatment of DNase I to remove genomic DNA contamination ...
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bioRxiv - Genetics 2021Quote: ... qPCR was carried out using BioMaster HS-qPCR SYBR Blue (2×) reagent kit (Biolabmix; https://biolabmix.ru/en/) and CFX96™ Real-Time PCR Detection System (Bio-Rad). We used the following thermal cycling conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total RNA and oligo (dT) primers were reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed as a two-step RT-qPCR by first synthesizing oligo(dT) primed cDNA using the iScript™ Select cDNA Synthesis Kit (Biorad), then running ddPCR via the standard EvaGreen program on a Biorad QX200 ddPCR system and analyzing in Quantasoft software (see Table S2 for primer sequences).
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and TNF-α were quantified using real-time PCR and the SsoAdvanced Universal SYBR Green Supermix Kit (BIO-RAD, Hercules, CA). β-actin was used as an internal control ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (280 ng per sample) was used to generate cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). The cDNA was used as a template for qRT-PCR with SsoFast EvaGreen Supermix (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... the volume of BioID lysis buffer added was based on protein concentration determination using the DC Protein Assay Kit from Bio-Rad on the 10% aliquot samples lysed in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR expression analysis of selected candidates was performed using cDNAs produced from the iScript cDNA Synthesis Kit (Bio-Rad) in a LightCycler 480 system (Roche ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was produced using the High Capacity RNA-to-cDNA Kit (Applied Biosystem) and the cDNA was pre-amplified with SsoAdvanced PreAmp Supermix (Bio-Rad) using primers from Integrated DNA Technologies or Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 μg of total RNA sample (100 ng/μl) was retrotranscribed in stable cDNA with iScriptTM Advanced cDNA Synthesis Kit (Bio-Rad). Genes of interest were amplified with Itaq UniversSYBR Green (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s protocol ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: Membrane and cytosolic proteins fractions from MS were extracted using ReadyPrep™ Protein Extraction Kit (Membrane I) (Bio-Rad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The cDNA samples were used in a qRT-PCR experiment employing the iQ SYBR Green Supermix Kit (Bio-Rad, Munich, Germany). PCR was conducted in a CFX96 RT-PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: Agarose plug embedded nuclei were Proteinase K treated for two days followed by RNAse treatment (Biorad CHEF Genomic DNA Plug Kit). DNA was recovered from agarose plugs according to IrysPrep™ Plug Lysis Long DNA Isolation guidelines (BioNano Genomics) ...
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bioRxiv - Genetics 2019Quote: ... Concentration of protein lysates was determined using either the Pierce BCA protein assay kit (Pierce) or the DC Protein Assay (Bio-Rad) following the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... An amount of 500-1000 ng of purified total RNA was then subjected to reverse transcription using the iScript cDNA synthesis kit (Bio-Rad), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... 800 ng of purified RNA was used as a template to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). End-point PCR was then conducted using primer sets EH13/EH14 and EH1/EH2 ...
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bioRxiv - Neuroscience 2019Quote: ... total protein concentration of the supernatant was assayed by using Bio-Rad protein assay kit (Bio-Rad, Marnes-la-Coquette, France). Equal amounts of each protein sample were separated on NuPAGE Bis-Tris or Tris-Acetate gels and transferred to nitrocellulose or PVDF membranes ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: Quantitative real-time (qRT)-PCR was performed using the iCycler-iQ system and the iQ SYBR Green Supermix Kit from Bio-Rad. The primer pairs used to amplify the target genes were designed using the Primer3 software (http://bioinfo.ut.ee/primer3/ ...
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bioRxiv - Plant Biology 2019Quote: ... and the Takyon No ROX SYBR 2X MasterMix blue dTTP qPCR Kit (both Eurogentech) for quantitative real time PCR using a CFX96 detection system (Bio-Rad). The previously described reference genes Protein Phosphatase 2A (PP2A ...
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bioRxiv - Cell Biology 2019Quote: ... RNA concentration and quality were determined using Nanodrop (PeqLab) and cDNA was synthesized with the iScript cDNA synthesis kit (Bio-Rad) following manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We then performed quantitative PCR (qPCR) using 2 ng of cDNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: Total RNA from SIM-A9 cells with varying treatment conditions was reverse transcribed to cDNA libraries using the iScript cDNA Synthesis Kit (Bio-Rad) with a 1 μg RNA input ...
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bioRxiv - Physiology 2019Quote: ... The cDNA synthesis was performed with 1μg of total RNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Bioengineering 2021Quote: ... The HRP conjugated secondary antibodies were visualized by using an enhanced chemiluminescence detection kit and ChemiDoc Touch Imaging System (Image Lab, Bio-Rad).
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bioRxiv - Evolutionary Biology 2020Quote: ... DNase-treated RNA was used to perform cDNA synthesis in a final volume of 20 μl using the iScript cDNA synthesis kit (Biorad, USA). The reaction was performed at 46°C for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The chemiluminescence of the membranes was developed using Super Signal Femto Substrate Kit (Pierce) and developed using ChemiDoc Touch Imager (Bio-Rad). Primary antibodies and conditions used are as follows ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was performed using the TaqMan Fast Advanced Cells-to-CT kit with the FX96 Real-Time PCR Detection System (Bio-Rad) with the TaqMan probes listed in table S15 ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK). Spots were enumerated using an AID ELISpot reader and software (AID).
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bioRxiv - Systems Biology 2021Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of purified RNA were used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad, Irvine, CA), and real-time quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2021Quote: ... A preamplification step that amplifies the amount of cDNA available for downstream RT-qPCR analysis was conducted using SsoAdvanced PreAmp Kit (BioRad, 1725160). RT-qPCR was performed using TaqMan Gene expression assays (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Biochemistry 2020Quote: ... the protein concentration was determined using the BioRad DC microplate assay protocol (BioRad DC Protein Assay Kit, BioRad, Hercules, California #5000112). Protein Standards and samples were run on NuPAGE 10% Bis-Tris Gels (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... N2a cells were harvested at the same time points for reverse-transcriptase (RT) PCR generation using iScript cDNA Synthesis kit (Bio-Rad), and quantitative PCR (QPCR ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated and measured as detailed above and then converted to cDNA using iScript cDNA Synthesis Kit (Bio-Rad #1708891). Quantitative RT-PCR was carried out with TaqMan Gene Expression Assay reagents ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad). GAPDH was used as the invariant housekeeping gene internal control.
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bioRxiv - Plant Biology 2020Quote: ... and the Takyon No ROX SYBR 2X MasterMix Blue dTTP qPCR Kit (both Eurogentech) for quantitative real-time PCR using a CFX96 detection system (Bio-Rad). Transcript data was normalized to UBQ10 ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were stored at −80°C until cDNA synthesis using the iScript™ gDNA Clear cDNA Synthesis Kit according to the manufacture’s guidelines (BIO-RAD). A qPCR-based assay was used to detect and quantify PfCSP transcripts (Kefi et al. ...
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bioRxiv - Genomics 2021Quote: ... 30-40 ng of RNA was used to make cDNA using the iScript Adv cDNA Syn Kit (Bio-Rad life Sciences). The RNA was diluted 1:50 in water for ddPCR analysis ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM or 3.2 μM of SAM-3H3 were used and the reaction was cleaned with either a PCR purification kit or Bio-Spin P30 columns (Bio-rad). The reactions were incubated for 2 hrs at 37 °C ...