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Citations for Bio-Rad :
7551 - 7600 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (Clarity Western ECL Substrate, Bio-Rad, California, USA) and visualized with Chemidoc Touch imaging system (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then resolved by denaturing PAGE using 4-20% acrylamide gels (Bio-Rad, Hercules, CA, USA). Proteins were then transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... Methanol-activated PVDF membranes were used for protein transfer via the Trans-Blot Turbo System (Bio-Rad). The high molecular weight ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were transferred onto a nitrocellulose membrane with a Trans-Blot Turbo Transfer System (Bio-Rad). Proteins were detected with rabbit anti-His (1/5,000 ...
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bioRxiv - Microbiology 2024Quote: ... Thirty micrograms of protein from each cell lysate were mixed with 4x Laemmli sample buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ... purified protein samples were then diluted into Laemmli Sample Buffer (Bio-Rad, Hercules, CA; Cat. No. 1610747) supplemented with 2.5% beta-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to a nitrocellulose membrane using a Trans-Blot Turbo system (Bio-Rad, Hercules, CA) and probed with anti-His antiserum (Qiagen ...
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bioRxiv - Biophysics 2024Quote: ... Protein-specific bands were revealed using enhanced chemiluminescent Clarity Western ECL Substrate (Bio-Rad, Hercules, California, USA) and visualized on a Chemidoc imaging system ...
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bioRxiv - Immunology 2024Quote: ... 20 ng 230CMB was loaded per well and the Precision Plus Dual Color protein marker (Bio-Rad) was used as size standard.
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bioRxiv - Immunology 2024Quote: ... 10µg protein per sample was separated by gel electrophoresis on TGX Stain-Free Precast Gels (BioRad 4568083). PHB1 (Cell Signaling #2426 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were prepared for western blot analysis by adding 4x sample buffer (Bio-Rad, Cat. #1610747) and 1 mM 2-mercaptoethanol (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty µg of nuclear proteins were separated on precast 4-15% SDS-polyacrylamide gels (Bio-Rad Laboratories), and then transferred onto polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
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bioRxiv - Plant Biology 2024Quote: ... and 15ug of each sample was loaded into a 4-20% SDS-PAGE precast protein gel (BioRad). Proteins were fractionated by electrophoresis and then transferred to PVDF membranes (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Thirty micrograms of protein lysate were resolved on 4-12% TGX Acrylamide Stain-Free gels (Bio-Rad). The Stain-Free gels were imaged prior to transfer to PVDF (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Band densities were normalized to the total amount of protein loaded per lane using Sypro Ruby (BioRad).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins were transferred to a PVDF membrane using the Trans-Blot Turbo system (Bio-Rad Laboratories, USA). The membrane was washed with TBST solution and then blocked with Quick Blocking Buffer (NCM biotech ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane using the Trans-Blot Turbo transfer system (Bio-Rad). The membranes were blocked with 5% BSA in tris-buffered saline - 0.1% Tween (TBST ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 12% polyacrylamide gels and transferred to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2024Quote: ... 30µg of protein was added per well and run on a 4-20% pre-cast gel (Biorad). Membranes were probed with α-ALDH2 (Proteintech 15310-1-AP ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Bait and prey proteins were loaded on separate gels (Bio-Rad 4-20% precast gels cat. #4568094). Proteins were transferred to nitrocellulose membrane and immunoblotted as described above ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The specific protein bands were visualized with a secondary antibody (anti-rabbit IgG-HRP conjugate; Bio-Rad) using a chemiluminescent substrate (Amersham ECL Western Blotting Detection Reagents ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to nitrocellulose membranes using a Trans-Blot® Turbo blot transfer pack (Bio-Rad). Nitrocellulose membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF using either a Trans-Blot Turbo system (with Turbo transfer buffer) (BioRad) or by traditional wet tank transfer using CAPS Buffer with 10% Methanol (for DNA-PKcs) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate (20 µg) was separated on 4-20% Mini-PROTEAN® TGX Stain-FreeTM protein gels (Biorad) and transferred onto Immun-Blot® Low Fluorescence PVDF membrane (Biorad) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10-20 mg protein per lane was electrophoresed on 4-15% precast gradient gels (Bio-Rad). Based on the protein concentration and sample volumes ...
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bioRxiv - Microbiology 2024Quote: ... T3SS effectors/proteins from supernatant were resuspended in Urea 8M and mixed with Laemmli buffer (Bio-Rad). OD600 of bacterial cultures was used to normalize the amount of sample for loading into the SDS-PAGE.
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bioRxiv - Neuroscience 2024Quote: ... then proteins were separated by SDS-PAGE and transferred to a PVDF membrane (BIO-RAD, 162-0177). The membrane was blocked with 5% skim milk in PBS containing 0.05% Tween 20 and then incubated with rabbit anti-RhoA (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... with 10% 2-mercaptoethanol and ran on precast Any kD SDS-PAGE protein gels (BioRad, cat. # 4569034). Proteins were then transferred onto low-fluorescence PVDF membrane (BioRad cat ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then transferred using Trans-Blot Turbo Midi 0.2μM Nitrocellulose Transfer Packs (Bio-Rad; Cat# 1704159) with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: The TNN lysates were precleared with Protein A/G magnetic beads (Bio-Rad or Thermo Fisher Scientific) for 1 hour at 4°C on the rotor ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the clarified yeast extract was ∼ 80 mg/mL by Bradford assay (BioRad, USA). The extract was stored at - 80 °C until reconstitution experiments were performed.
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bioRxiv - Neuroscience 2024Quote: ... Protein samples (30-50 μg) were separated using Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). After blocking ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by SDS-PAGE using pre-cast 4-20% Mini-PROTEAN TGX Gels (Bio-Rad) and transferred onto a 0.45 µm PVDF membrane (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred on a nitrocellulose membrane with the Trans-Blot Turbo™ Transfer System (Bio-Rad). Total protein load per well was evaluated after imaging the membrane under UV light ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µL of Precision Plus Dual Xtra Protein Standards (2–250 kDa) were used (Bio-Rad Laboratories). Oligomerization patterns were visualized using silver staining.
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bioRxiv - Biophysics 2024Quote: ... Proteins were resolved using SDS-PAGE and transferred to a nitrocellulose membrane via wet electroblotting (Bio-Rad). The membrane was blocked with 5% milk (Americanbio ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was done using the Bradford method (Cat #5000006, Bio-Rad Laboratories Inc., Hercules, CA, USA). Western blotting was performed as previously described.29 The detailed protocol is given in Supplementary Methods.
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bioRxiv - Biophysics 2024Quote: ... 550 mM NaCl) and bound protein eluted with 35 µL of 2x SDS-PAGE sample buffer (BioRad) containing 100 mM dithiothreitol ...
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bioRxiv - Biophysics 2024Quote: ... The samples were loaded onto a 4-20% Mini-PROTEAN precast protein gel (Bio-Rad, Hercules, CA) and ran on a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... The samples were loaded onto a 4-20% Mini-PROTEAN precast protein gel (Bio-Rad, Hercules, CA) and ran on a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... The samples were loaded onto a 4-20% Mini-PROTEAN precast protein gel (Bio-Rad, Hercules, CA) and ran on a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins of interest were detected using HRP-conjugated secondary antibodies against mouse (1:7500, Bio-Rad Laboratories). The proteins were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... The excess DTT was removed by passing the protein solution through Econo-Pac 10G desalting column (Biorad). The concentration of the protein solution was measured via UV-vis absorption at 280 nm and the fluorophore was added in a 1:1 protein-fluorophore molar ratio for labeling ...
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bioRxiv - Cell Biology 2024Quote: ... and protein samples were transferred onto a polyvinylidene difluoride (PVDF) membrane (#1704157; Bio-Rad, Hemel Hempstead, UK) using the Trans-Blot Turbo Transfer System (#1704150 ...
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bioRxiv - Biochemistry 2024Quote: ... and the resultant protein pellets were resuspended in non-reducing 4X loading buffer (Bio-Rad, UK, #1610747). A sample boiling step was omitted as it could inadvertently break disulfide bonds ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were size separated by SDS-PAGE and transferred onto nitrocellulose membranes of 0.2 µm (Bio-Rad). Immunoblots were blocked with 5% non-fat milk in TBS-T containing 0.1% Tween-20 or in PBS-T containing 0.05-0.1% Tween-20 for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were then transferred onto a onto Polyvinyl difluoride transfer membranes (Immuno-Blot PDVF Membrane, Biorad, UK). Membranes were blocked in 5% dried skimmed milk with 0.1% Tween-20 (TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration of each sample was determined using a colorimetric assay (Bio-Rad, Hercules, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein were subjected to SDS-PAGE and transferred onto 0.2µm PVDF membranes (Bio-Rad). Membranes were blocked for 1 hour with a 5% BSA solution (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The agarose pellets were then reconstituted with 40 ul of 2x Laemmli protein buffer (1610747, Bio-rad), followed by Western blot analysis to detect ubiquitinated TFE3.