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Citations for Bio-Rad :
701 - 750 of 6179 citations for ssc mir 487b RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... The variations in mRNA levels of the target genes were established by relative RT-qPCR with a CFX96TM Real-Time PCR Detection System and the iQ™ SYBR Green Supermix (Bio-Rad) for the detection of single PCR product accumulation ...
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bioRxiv - Molecular Biology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, USA). The detection limit was calculated to be 1000 copies per reaction ...
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bioRxiv - Bioengineering 2023Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... qPCR was carried out on a C1000 RT-PCR using iTaq Universal SYBR Green Supermix (all from Bio-Rad, Hercules, CA, USA) and primers targeting Prdm1 (forward 5’-TTC TCT TGG AAA AAC GTG TGG G-3’ and reverse 5’-GGA GCC GGA GCT AGACTT G-3’) ...
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bioRxiv - Neuroscience 2024Quote: The expression of mRNAs was quantified by real-time RT-qPCR on a CFX ConnectTM Real-Time PCR System (Bio-Rad Laboratories) in the presence of SsoAdvanced universal SYBR Green Mix (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was generated with 400ng of RNA with iScript RT Supermix for RT-qPCR (BioRad). GAPDH and Satb2 primers were from (Gong et al. ...
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bioRxiv - Cell Biology 2021Quote: ... were amplified using Phusion High-Fidelity PCR MasterMix (primer sequences are listed in Additional File 2) and a C1000 thermocycler (Bio-Rad Life Science). The peak sequences were amplified for 98 °C for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Droplet digital PCR (ddPCR) primer/probe assays for copy number variation (CNV) were obtained from Bio-Rad (mouse ND1/HEX, mouse ActB/FAM). The ddPCR mix was as follows ...
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bioRxiv - Cell Biology 2022Quote: ... Primer designs (see Key Resources Table) and qRT-PCR setup was performed using SsoAdvanced Universal SYBR® Green Supermix (1725274, Bio-Rad, USA) following manufacturer’s protocol in 10 μl reactions (5 μl Supermix ...
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bioRxiv - Physiology 2019Quote: ... PCR was performed with 20ng of cDNA and the corresponding primer pairs using an iCycler Real-Time Detection System (BioRad Laboratories, Hercules, CA). The relative quantity of mRNA was determined using the comparative Ct method and expression levels of P2Y2 receptor mRNA were normalized to ribosomal 18S expression.
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bioRxiv - Microbiology 2020Quote: ... The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38) before running on a CFX96TM real-time PCR cycler (BioRad, Hercules, CA, USA) under cycle conditions of 42°C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNA was amplified by qPCR with specific primers (Supplementary Table S1) using the C1000™ thermal cycler with CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories). The relative expression of genes was analyzed quantitatively by the ΔΔCt method ...
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bioRxiv - Plant Biology 2023Quote: Real-time quantitative PCR was performed using gene-specific primers (Table S1) for selected genes on CFX96 Touch™ Real-Time PCR Detection System (Biorad, California, USA). The amplification conditions were ...
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bioRxiv - Microbiology 2024Quote: Each vaginal and plasmid control DNA sample were amplified in triplicates using species-specific primers and fluorescent probes on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad, California, USA). The specific primer and probe sequences ...
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bioRxiv - Cell Biology 2024Quote: ... Primer designs (see Key Resources Table) and qRT-PCR setup was performed using SsoAdvanced Universal SYBR® Green Supermix (1725274, Bio-Rad, USA) following manufacturer’s protocol in 10 μl reactions (5 μl Supermix ...
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bioRxiv - Immunology 2023Quote: ... All primers were from Bio-Rad. Some results are also normalized to baseline conditions as the figure legends indicate ...
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bioRxiv - Molecular Biology 2019Quote: ... EtOH precipitation was performed and DNA was additionally cleaned up on Micro Bio-Spin 6 chromatography columns in SSC (BioRad) as purity was essential for oxidation of hmC ...
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bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
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bioRxiv - Physiology 2019Quote: ... Real time polymerase chain reaction (RT-PCR) was performed with the CFX96 or CFX384 Touch™ Real-Time detection system (Bio-Rad Laboratories), using a SensiMix™ (BioLine ...
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bioRxiv - Molecular Biology 2019Quote: ... Gene expression was quantified using Real-time (RT-qPCR) with SsoAdvanced™ Universal SYBR® Green Supermix on a CFX Connect™ Real-Time PCR Detection System (BioRAD). The primers used for RT-qPCR are available upon request.
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bioRxiv - Physiology 2019Quote: ... Transcript levels were analyzed by real-time polymerase chain reaction (RT-PCR) (fluorescence detection of SYBR green) in an iCycler iQ apparatus (Bio-Rad, Hercules, CA), with normalization against 18S as an endogenous control ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR (RT-qPCR) analysis was carried out using a CFX384 Real-Time System (C 1000 Touch Thermal Cycler, Bio-Rad, Hercules, California) using SensiFAST SYBR No-ROS kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... MNV or HAV RNA recovered by the ISO 15216-1 and PGM-MB or HAV antibody methods was quantified using the One-Step dd-RT-PCR Advanced Kit for Probes (Bio-Rad Laboratories, Ltd.) according to manufacturer’s instructions and as described previously (23 ...
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bioRxiv - Plant Biology 2019Quote: ... genes that encode for the biosynthetic enzymes along the JA-pathway were evaluated by quantitative RT-PCR using the iQ SYBR Green Supermix (Bio-Rad; CAT: 1708882) reagents ...
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bioRxiv - Neuroscience 2019Quote: ... BDNF exon IV and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA (as a reference gene) were amplified using the iScript RT-PCR iQ SYBR Green Supermix (BIORAD, Hercules, CA, USA) in a real-time quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2020Quote: ... was used for quantification and all PCR reactions were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad Cat# 1864022). Primers used were previously described in (25) ...
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bioRxiv - Plant Biology 2022Quote: ... The transcript levels of selected genes were measured by RT-qPCR using the CFX96® Real □ Time PCR System (BioRad, http://www.bio□rad.com/) and the 2 × T5 Fast qPCR Mix (SYBRGreenI ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA). qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and PCR amplification were performed in a single reaction using the One-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, CA, USA) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR (RT-qPCR) analysis was performed using a CFX384 Real-Time System (C 1000 Touch Thermal Cycler, Bio-Rad, Hercules, California) using SensiFAST SYBR No-ROS kit (Bioline ...
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bioRxiv - Immunology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, United States). The limit of detection was calculated to be 10 copies per reaction.
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bioRxiv - Immunology 2020Quote: ... and automatic exposure set up on ChemiDoc imaging system (BioRad).
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bioRxiv - Bioengineering 2021Quote: ... Pre-amplification primers and regular primers used were PrimePCR™ SYBR®® Green Assays (Bio-Rad) as listed in Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... All primers in the study were commercially purchased PrimePCR™ SYBR® Green assay primers (Bio-Rad). For each sample ...
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bioRxiv - Microbiology 2021Quote: ... Multiplex RT-qPCR assay were carried out using Reliance One-Step Multiplex RT-qPCR Supermix (BioRad) or LightCycler Multiplex RNA Virus Master (Roche) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RT-qPCR was performed using iTaq Universal One-Step RT-qPCR Kits (Bio-Rad, cat.# 1725150). RT-qPCR primers were designed using Primer359 and are listed in Supplementary Table 13 ...
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bioRxiv - Microbiology 2023Quote: ... one-step RT-ddPCR (1-Step RT-ddPCR Advanced Kit for Probes, Bio-Rad, Hercules, CA) was used to determine absolute quantification of N and S vRNA copy numbers in culture supernatants and cell lysates using the OC43 ...
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bioRxiv - Biochemistry 2020Quote: ... Random hexamers were used for cDNA synthesis primers and qRT-PCR was then carried out in triplicate using Bio-Rad iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules CA, #1725120) and measured with the CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... was performed with gene-specific primers (Supplementary Table 1) using EF1α as internal reference using the CFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) as previously described in (Zandalinas and Mittler ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed with KOD SYBR qPCR Mix (TOYOBO) and primers (Supp Table 7) using the CFX Connect Real-Time PCR Detection System (Bio-Rad; Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was subsequently performed using the iTaq Universal SYBER Green Supermix using the primers listed in Supplemental Table 1 on a CFX96 Real-Time PCR Detection System (Bio-Rad, Richmond, CA, USA). Data were normalized using GAPDH as internal control.
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bioRxiv - Immunology 2021Quote: ... Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad). ß-actin (ACTB ...
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bioRxiv - Developmental Biology 2022Quote: ... HRMA is a powerful tool to screen indel mutations or single nucleotide polymorphism in the samples and it was performed on the genomic DNA with specific primers listed in Table S1 using the CFX96 Real-Time PCR Detection System and the Precision Melt Analysis software (Bio-Rad Laboratories, Hercules, CA). HMA was performed with PAGE to verify the genotyping results of HRMA ...
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bioRxiv - Molecular Biology 2019Quote: ... (iv) BP2_DM1_H+ (iScript RT, Bio-Rad) consisted of 5 DM1 and 6 control samples ...
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bioRxiv - Molecular Biology 2020Quote: ... and iScript RT Supermix (Bio-Rad) with an input of 250 ng RNA (determined by Nanodrop ...
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bioRxiv - Immunology 2022Quote: ... for RT-qPCR from Bio-Rad. qPCR reaction was set up by using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The final quantification of miR-122-5p copies was done using the QuantaSoft™ Analysis Pro software (BIORAD, USA) according to the Poisson law ...
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bioRxiv - Developmental Biology 2019Quote: ... and nuclease-free water controls were spotted on a pre-wetted (10X SSC buffer) nylon membrane using a Bio-Dot Microfiltration Apparatus (Bio-Rad). The blotted membrane was air-dried and UV-cross-linked at 1200 J/m2 using a UV Stratalinker 1800 (Stratagene) ...
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bioRxiv - Genetics 2020Quote: ... twice in 200 μl nuclease free water and once in 100μl 1x SSC (150 mM NaCl, 15mM sodium citrate, pH 7.0, BIO-RAD Laboratories, Inc.) and finally resuspended in 50 μl 1X SSC ...
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bioRxiv - Molecular Biology 2023Quote: ... single yeast cells were gated on a forward-scatter area (FSC-A) by side-scatter area (SSC-A) plot around the clustered population (P1) on a ZE5 Cell Analyzer (Bio-Rad). P1 was then gated on a side-scatter width (SSC-W ...