Labshake search
Citations for Bio-Rad :
701 - 750 of 930 citations for Trypan Blue Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... 0.02% bromphenol blue) for 15min at 50°C and 5μg of protein were separated on a 4-20% SDS PAGE (Biorad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
-
bioRxiv - Microbiology 2021Quote: ... Samples taken from individual fractions were analysed on denaturing SDS polyacrylamide gels (8 %) stained with Coomassie Brilliant Blue (Bio-Rad). Fractions containing pure (>90 % ...
-
bioRxiv - Biochemistry 2021Quote: ... the gels were either stained with One-Protein Blue gel stain (#21003-1L, Bioticum) or transferred onto PVDF membranes for immunodetection using TurboBlot system (Biorad).
-
bioRxiv - Microbiology 2021Quote: ... Bradford Protein Assay Reagents 1 and 2 and Precision Plus Protein All Blue Prestained Protein Standard were from Bio-Rad. The anti His-tag mouse antibody was from GenScript (cat no ...
-
bioRxiv - Biophysics 2021Quote: SDS-page gel electrophoresis analysis (12.5% running gel, 4% stacking gel stained with Coomassie brilliant blue R-250 Bio-Rad) was carried out for checking the yield of PsbS at every step of overexpression ...
-
bioRxiv - Plant Biology 2022Quote: ... Eluted protein samples were checked by SDS-PAGE and Coomassie brilliant blue staining (Bio-Safe™ Coomassie Stain, Bio-Rad). The protein concentration was determined with the Bradford method (Quick Start™ Bradford Reagent ...
-
bioRxiv - Cancer Biology 2022Quote: ... Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad) staining.
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were visualized by Coomassie blue staining and ATG3 monomer and dimer were quantified using the ImageLab software (Bio-Rad) and the sample treated with 20 mM DTTred and 0 mM DTTox (Eh= -∞ ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were analyzed by SDS-PAGE and purities determined by densitometry of Coomassie Blue-stained gels (Image Lab Software, BioRad). Protein batches used in this work were ≥ 90% pure.
-
bioRxiv - Bioengineering 2023Quote: ... cell-seeded scaffolds were transferred into a new well and 200μL of fresh media containing 10% Alamar blue (cat. BUF012A Bio-Rad) was added to each scaffold ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were then separated on 10-20% SDS-polyacrylamide gels and were visualized by staining with Coomassie blue (Bio-Rad).
-
bioRxiv - Plant Biology 2023Quote: RT-qPCR reaction mixtures were set up according to the manufacturer’s instructions (Blue ŚGreen, Biozym) and reactions were carried out on a CFX96TM Touch thermocycler (Bio-Rad). As a control for contamination with genomic DNA ...
-
bioRxiv - Biochemistry 2023Quote: ... Blue Native PAGE gel experiments were performed in a cold room (4 °C) using 10% precast gels (BioRad Mini-PROTEAN) and run at 170 V for 55 minutes ...
-
bioRxiv - Physiology 2023Quote: ... The gels were then stained with Colloidal Coomassie Brilliant Blue (CBB) and scanned using a GS-800 scanner (BIO-RAD). Protein spots were analyzed using PDQuest software (BIO-RAD ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples of supernatant were separated by SDS-PAGE and resulting bands were visualized using Coomassie Brilliant Blue R-250 (Biorad) solution ...
-
bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies and dilutions were used for 1 hour at room temperature: goat anti-rabbit StarBright Blue 700 (1:2,500; Bio-Rad), goat anti-rabbit IRDye 800 CW (1:5,000 ...
-
bioRxiv - Biochemistry 2023Quote: ... pre-cast Tris-Glycine gels and stained with Coomassie Brilliant Blue stain or transferred for western blotting analyses onto 0.4 µm nitrocellulose membrane (Bio-Rad) in Tris-Glycine buffer with 20% methanol for 1 hour at 100 V ...
-
bioRxiv - Genetics 2023Quote: ... bands were visualized with methylene blue staining and imaged on a Gel Doc XR+ Molecular Imager with Image Lab software (BioRad). Probe templates containing the T7 promoter were amplified using PCR ...
-
bioRxiv - Biochemistry 2023Quote: ... It was first clarified by centrifugation at 10,000 g for 30 min and loaded onto a 10-mL self-assembled Affi-Gel Blue gravity column (Bio-Rad). The column was sequentially washed with 50 mL resuspension buffer containing 0.3 M NaCl ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
-
bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBST and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
-
bioRxiv - Cell Biology 2022Quote: ... Excess dye was removed by two consecutive buffer exchanges through Micro Bio-Spin P-6 desalting columns (Bio-Rad). Protein concentration and labeling efficiency were estimated using a NanoDrop Microvolume Spectrophotometer.
-
bioRxiv - Cancer Biology 2020Quote: ... The supernatant was transferred to eppendorf and mixed with 2X loading dye (2X Lammeli Buffer, Bio-Rad, No. 1610737) + beta-Mercaptoethanol (Sigma) ...
-
bioRxiv - Plant Biology 2021Quote: ... The concentration of the purified protein extracts was measured using the Bradford method (Protein Assay Dye Reagent, Bio-Rad). Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels ...
-
bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad).
-
bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad). One unit of Acetyl-CoA Carboxylase activity is defined as the enzyme generates 1 nmol of PO43-per hour.
-
bioRxiv - Biophysics 2022Quote: ... The protein-dye conjugate was purified by 3 rounds of size exclusion chromatography (Bio-Spin 6 column, BioRad, CA) and then aliquoted in 5 μL portions and stored at -80C until required.
-
bioRxiv - Synthetic Biology 2022Quote: Protein concentrations of the cell lysates were determined using a Bio-Rad Protein Assay Dye Reagent (Bio-Rad, 5000006). Volumes of lysates containing equal amounts of total protein were boiled at 100°C for 5 minutes after addition of 1/4 volume of 4x SDS-PAGE Loading Solution (250 mM Tris ...
-
bioRxiv - Biophysics 2022Quote: ... Excess dye was removed via size exclusion chromatography using an ENrich™ SEC 650 10 x 300 Column (BioRad).
-
bioRxiv - Biochemistry 2020Quote: ... 10 µL of ∼500 nM RNA was mixed with 2 µL of RNA loading dye and loaded into a 1.0 cm well PAGE casting plate (BioRad). Urea-PAGE (11.2% ...
-
bioRxiv - Pathology 2021Quote: ... The concentration of proteins was determined using a Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, #5000006). A total of 15 μg of protein from each sample were mixed with NuPAGE™ LDS Sample Buffer (Thermofisher ...
-
bioRxiv - Developmental Biology 2019Quote: ... Lysates were cleared at 16,000 g and protein concentration determined using the Bio-Rad Protein Assay Dye (Bio-Rad). Lysates were resolved by 8% SDS-PAGE ...
-
bioRxiv - Biochemistry 2019Quote: ... reaction aliquots were quenched by addition to SDS/PAGE loading dye and samples were loaded onto a Criterion 16.5% TRIS/TRICINE gel (BioRad) along with [14C]BSA counting standards (American Radiolabelled Chemicals ...
-
bioRxiv - Plant Biology 2021Quote: ... no ROX using SYBRGreen as a dye in a 384 well plate on a CFX96 cycler (BioRad, Hercules, CA). The total volume of the reaction mix was 8 μL containing 250 μM of the 1:1 pre-mixed primer ...
-
bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad).
-
bioRxiv - Cell Biology 2021Quote: Total protein concentration of each cell lysate sample was determined by Bradford assay with Protein Assay Dye (Bio-Rad) on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
-
bioRxiv - Microbiology 2022Quote: ... total protein was visualised post-transfer to PVDF membranes on ChemiDoc using Stain-free fluorescent dye (Bio-Rad Laboratories). For analyses of protein band intensities ...
-
bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations were determined by Bradford Assay with the Bio-Rad Protein Assay Dye reagent concentrate (Bio-Rad) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Quantitative PCR was performed using SYBR Green dye (CFX Connect Real-Time System, Bio-Rad Laboratories, Inc., Hercules, CA). Fold-changes in gene expression were calculating by normalizing data to Gapdh as a house-keeping gene and values were presented relative to uninfected cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... The protein concentrations of sample supernatants were measured with the Bio-Rad protein assay dye reagent (Bio-Rad, 5000006). Equivalent amounts of protein samples were mixed with Laemmli buffer (100 mM Tris-HCl pH 6.8 ...
-
bioRxiv - Genomics 2023Quote: ... Excess dye was removed using Micro Bio-Spin® Columns with Bio-Gel® P-6 (cat:7326221, BioRad) by centrifugation at 1000g ...
-
bioRxiv - Plant Biology 2023Quote: ... Ten µl of the supernatant were used to determine protein concentrations by the dye-binding Bradford assay (Bio-Rad). Twenty-five µg of total proteins in a final volume of 100 µl were pipetted into a white 96 well plate (Greiner Bio-One ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
-
bioRxiv - Cell Biology 2023Quote: ... 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad). Proteins were transferred to the PVDF membrane using a Trans-blotter (Biorad) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cells were centrifuged for 15 min at 15,000 x g at 4°C and the supernatant was quantified with Quick Start Bradford 1X Dye Reagent (BioRad), using an albumin curve to determine the concentration of protein to be placed ...
-
bioRxiv - Cancer Biology 2024Quote: ... Total protein concentration was determined by Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with StarBright Blue 700 Goat Anti-Mouse IgG secondary antibody (1:2500, Bio-Rad, Cat# 12004159, RRID: AB_2884948), StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500 ...
-
bioRxiv - Cell Biology 2020Quote: ... The eluted fractions were analyzed on a SDS-PAGE gel and subsequently the peak fractions detected on the Coomassie blue (Biorad 1610400) stained gel were pooled and dialyzed in a 10 kDa SnakeSkin™ dialysis tubing (Thermo Fisher 68100 ...
-
bioRxiv - Genetics 2021Quote: ... qPCR was carried out using BioMaster HS-qPCR SYBR Blue (2×) reagent kit (Biolabmix; https://biolabmix.ru/en/) and CFX96™ Real-Time PCR Detection System (Bio-Rad). We used the following thermal cycling conditions ...
-
bioRxiv - Biochemistry 2022Quote: ... 25% v/v glycerol and 0.25% v/v bromophenol blue) was added to each sample prior to resolution on 12 % Mini-PROTEAN TGX Gels (Bio-Rad) by polyacrylamide gel electrophoresis (PAGE ...