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Citations for Bio-Rad :
701 - 750 of 784 citations for Atg4B Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... For detection, we used an anti-rabbit HRP-coupled secondary antibody (1:5000, Cell Signalling) and Western ECL substrate (#1705061, Bio-Rad. As a loading control ...
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bioRxiv - Cancer Biology 2024Quote: ... and followed by one-hour incubation with diluted secondary antibody (1706515 Goat Anti-Rabbit IgG-HRP Conjugate, Bio-Rad, Hercules, CA). Protein lysates from each cell line were analysed on multiple gels (four per cell line ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBS + 0.05% Tween-20 (PBS-T) and incubated in goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-rad) diluted 1:5000 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were washed and shaken in PBST thrice and then re-probed with a secondary goat anti-rabbit IgG-HRP-conjugated antibody (172-1019, Bio-Rad) at 1:10000 ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed then incubated for 1 hour at room temperature with secondary antibody (Goat Anti-Mouse Starbright Blue 520 and Goat Anti-Rabbit Starbright Blue 700, each at 1:2500, Bio-Rad). Blots were analyzed (ChemiDocTM MP Imaging System ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti mouse #1721011; Bio-Rad or goat anti rabbit #1706515; BioRad) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: Membrane was then washed three times with TBS-T and incubated with secondary antibody (Goat Anti-Rabbit IgG StarBright Blue 700 by Bio-Rad) diluted 1:3,000 in blocking solution for 45 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) or goat anti-mouse IgG-HRP conjugate (1:3000) (Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Genetics 2023Quote: ... primary antibodies – anti-TY1 and rabbit anti-HA (GTX115044) – and fluorescent secondary antibodies – anti-mouse Starbright Blue 520 (Bio-Rad 12005867) and anti-rabbit Starbright Blue 700 (Bio-Rad 12004162 ...
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bioRxiv - Cell Biology 2023Quote: ... Peroxidase-conjugated goat anti-mouse (170-6516) and peroxidase-conjugated goat anti-rabbit (1706515) secondary antibodies were purchased from Bio-Rad.
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bioRxiv - Microbiology 2023Quote: ... Signal detection was conducted with a goat anti-rabbit-HRP secondary antibody followed by development with Clarity ECL Substrate (Bio-Rad). Blots were imaged on Chemidoc XRS Imaging System (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Physiology 2023Quote: ... The secondary antibodies were goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP conjugate (Bio-Rad, Hercules, CA, USA) for western blotting ...
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Physiology 2024Quote: ... was used to wash the membranes before incubating with goat anti-rabbit horseradish peroxidase conjugated secondary antibody at 1:5000 dilution (1706515, Bio-Rad) for 90 min at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times with and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Physiology 2024Quote: ... Blotted bands were detected using HRP conjugated secondary antibodies goat anti-rabbit conjugated with HRP (#1706515, Bio-Rad, 1:15, 000). ImageJ was used to calculate the fluorescence density of each band ...
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bioRxiv - Neuroscience 2024Quote: ... The following day after washes with TBS-T and incubation with secondary antibodies (Goat Anti-Rabbit or Goat Anti-Mouse IgG (BioRad 1706516) 1:5000 dilution in 10% milk ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-capsid/p24 antibodies were developed following incubation with horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (BioRad; product code 1706515) or IRDye 800CW conjugated goat anti-rabbit IgG antibody (LI-COR ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in 10% normal donkey serum, and then stained in antibodies overnight (rabbit anti-Iba1, 1:500, Wako and CD68 (Biorad 1:100)) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Blotted membranes were washed three times with 0.05% Tween 20 in Tris-HCl buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against rabbit IgG (Bio-Rad Laboratories) at 1:10,000 dilution in Can Get Signal Solution 2 (TOYOBO ...
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bioRxiv - Immunology 2021Quote: ... in 0.1% BSA in 0.1% TBST and subsequently imaged using HRP-conjugated anti-rabbit antibodies (Bio-Rad Cat#172-1019, 1:10000) and Western Lightning Plus-ECL (PerkinElmer Cat#El103001EA ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was first probed with the indicated primary antibodies and then incubated with goat anti-rabbit (Bio-Rad, cat. no. 1706515) or goat anti-mouse (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... and followed by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat antimouse IgG (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed three times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1,706,515, 1:10,000) secondary antibodies for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for Western blotting were: Donkey anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA). Secondary antibodies used for immunofluorescence were ...
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bioRxiv - Microbiology 2022Quote: ... rabbit polyclonal anti-nsP1 (both validated in our laboratory) [11] and mouse monoclonal anti-GAPDH (Cat. # VMA00046, Bio-Rad, Hercules, CA, USA). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed twice with PBS and incubated with secondary antibodies (Desmin and MYOD1: anti-rabbit-HRP, BioRad 170-6515; Myogenin: anti-mouse HRP, BioRad 170-6516) 1:500 in PBS-1% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were incubated in secondary antibody for one hour at room temperature (anti-rabbit; Amersham, #NA934) (anti-mouse; Amersham, #NA931) and visualized with Clarity Western ECL Substrate (Bio-Rad, #1705061) on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were sectioned by freezing microtome at 50 μm intervals and immunohistochemical staining was performed for protein gene product 9.5 (PGP 9.5; rabbit polyclonal; AbD Serotec, a Bio-Rad Company, Kidlington, UK) using a free-floating protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% dry non-fat milk in PBST and probed using PRP8a antibody (1:500) and an HRP coupled anti-Rabbit secondary antibody (Biorad, 1:10000). All antibodies were diluted in 1% dry nonfat milk in PBST ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... blots were washed three times with 1X TBST and probed with the appropriate anti-rabbit (Cat# 170-6515, Bio-Rad, Hercules, CA) or anti-mouse (Cat# 7076 ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies were matched to the primary antibody and included HRP-conjugated goat anti-rabbit IgG (Bio-Rad Laboratories, Hercules CA, #1706515), goat anti-mouse IgG (Bio-Rad #1706516) ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were detected by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat anti-mouse IgG (BioRad ...
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bioRxiv - Microbiology 2020Quote: Bacterial overnights were diluted 1:1000 in LB and 50μl of each isolate was mixed with 25μl of Dulbecco’s Phosphate-Buffered Saline with calcium and magnesium (DPBS++) and 25μl baby rabbit complement serum (BRC) (Bio-Rad, C12CA, lot 148004). As controls ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515) for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... Blots were incubated with α-TPLATE appendage antibodies (rabbit) (Dejonghe et al, 2019) and imaged on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used for Western blotting were goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was visualised by chemiluminescence on Biorad ChemiDoc MP Imager after incubation with goat anti rabbit HRP conjugate (Biorad; 170-6515) or goat anti-mouse-HRP conjugate (Biorad ...
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bioRxiv - Physiology 2023Quote: ... The blots were washed four times with 0.1% tween TBS before being incubated for 1 h at room temperature with goat Anti-rabbit IgG HRP conjugated secondary antibody (Bio-Rad, USA) in 0.1% tween TBS buffer containing 3% BSA (Bovogen ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody incubation was done at room temperature for 1h (HRP-conjugated goat anti-rabbit IgG, 1:2000 dilution, Bio-Rad, 403005). Membranes were washed with Tris-buffered saline containing 0.05% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit IgG (H+L)-HRP conjugate (170-6510, Biorad, 1:10000) and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Microbiology 2024Quote: ... SigA proteins were detected using anti-SigA85 (1:10,000 dilution) combined with anti-rabbit-HRP secondary (Bio-Rad, #1706515, 1:5,000 dilution) antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... an affinity purified antibody generated against the SAX-7 cytoplasmic tail [gift of (Chen et al., 2001)] and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). For the loading control ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8 μm cryostat sections of C51 and F9 tumors stained with IgG (L19)-FITC (final concentration 2 μg/mL) and detected with a Rabbit anti-FITC (Bio-Rad; 4510-7804) and Donkey anti-Rabbit AlexaFluor 488 (Invitrogen ...