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Citations for Bio-Rad :
7301 - 7350 of 7983 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... membranes were de-stained and blocked for 5 minutes with EveryBlot Blocking Buffer (BIO-RAD; 12010020). Membranes were incubated with spastin primary antibody (Sp 3G11/1 ...
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bioRxiv - Cell Biology 2023Quote: ... Clarity Max Western ECL substrate was added to the membranes for 5 mins (Bio-Rad #1705062) and chemiluminescent signal imaged using a Chemidoc imager (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... DNA was extracted using 150 μL 5% Chelex 100 resin (Bio-Rad Laboratories, Hercules, United States) according to methods described previously (Lee et al 2012) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10 x BN loading dye (5% Coomassie Blue R-250 (Bio-Rad Laboratories, CA) in 500 mM 6-aminohexanoic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Each antibody was diluted in a 5% skim milk (MBcell, South Korea or Bio-Rad, USA) solution or TBST solution [20 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... 5% β-mercaptoethanol) and detected by Western blotting using StrepTactin-HRP (Bio-Rad catalog number 1610381). To prevent signal saturation ...
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bioRxiv - Immunology 2024Quote: ... Supernatants from each culture (5 µL) were directly pipetted onto 0.2-µm nitrocellulose membranes (Bio-Rad) and allowed to dry for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked with tris-buffered saline Tween (TBST) with 5% Blotting-Grade Blocker (Bio-rad) and incubated with primary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... centrifuged at 10 000 g for 5 min and mixed with 4x Laemmli buffer (Bio-Rad) and dithiothreitol (DTT) ...
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bioRxiv - Microbiology 2024Quote: ... Transfection was performed using ring-stage parasites (5-8% parasitemia) using a Gene Pulser Xcell (Biorad) electroporator.
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked with tris-buffered saline Tween (TBST) with 5% Blotting-Grade Blocker (Bio-Rad) and incubated with primary antibody ...
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bioRxiv - Biophysics 2024Quote: ... 5 µg of proteins/lane were dissolved in Laemmli Sample Buffer (Bio-Rad, Hercules, California, USA) added with 50 mM dithiothreitol ...
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bioRxiv - Neuroscience 2024Quote: ... before being air-dried overnight and then blocked in 5% skim milk (Bio-Rad, Hercules, CA) in phosphate buffer saline containing 0.1%Tween®20 (PBST)(Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and quantitative PCR (qPCR) was performed in an iQTM 5 Real Time-PCR Detection System (BioRad) with a SYBR Green mix (Takara and predesigned primers obtained from Sigma-Aldrich (KiCqStart® Primers) ...
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bioRxiv - Cell Biology 2024Quote: ... which were then blocked in 5% blocking grade skim milk (170–6404, Bio-Rad, Hercules, CA). Primary antibody was used at a dilution of 1:1000 ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted by mixing a small algal sample with 5% Chelex-100 resin (BioRad), incubated at 95°C for 20 min and 4°C for 20 min ...
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bioRxiv - Immunology 2024Quote: ... The blot was blocked in 5% non-fat skimmed milk (Blotting-Grade Blocker, Bio-Rad, USA) in 1X Tris-buffered saline with 0.1% Tween® 20 Detergent (TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... Excess antibody was washed away with 3 x TBST rinses before the membrane was incubated with anti-mouse secondary antibody (Bio-rad) conjugated to horseradish peroxidase at a 1:15000 dilution in TBST at 25°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... were added to this for a further 4 h before samples were centrifuged at 10,000 x g for 3 minutes and washed three times with RIPA buffer and re-suspended in laemmli buffer (Bio-Rad), boiled for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... tuberculosis Rv1630 and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) housekeeping gene was performed using 200 ng RNA in a T100 Thermal Cycler (Bio-Rad). The reaction was carried out with Reverse Transcription Reagents (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... separated using SDS-PAGE (4 to 12 % gradient gel at 150 V for 2.5-3 h) and transferred to a nitrocellulose membrane overnight (∼16 h) at 20 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5 % fat-free milk in 20 mmol/l Tris ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... membranes were rinsed 3 times with 1X TBST and the blots were incubated in Clarity ECL western blot substrate solution (Bio-Rad) for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The proportion of each splice isoform was quantified using the ImageJ software.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 2-3 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad, Hercules, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed with gl3 specific primers (Supplemental Table 3) and iQ™ SYBR® Green Supermix (BioRad, USA) and conducted on a BioRad CFX with 96-well reaction blocks under the following PCR conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before detection using Clarity Western ECL Substrate (Bio-Rad 170-5061) and imaging on a ChemiDoc MP (Bio-Rad 17001402).
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bioRxiv - Bioengineering 2022Quote: Samples were washed in PBS and fixed in 3% paraformaldehyde (PFA) for 15 minutes at room temperature (RT) and permeabilized by 0.5% Triton x-100 (Bio-Rad, USA) for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Specific primers against the genes of interest (Table 3) were designed and qPCR was performed to amplify the genes of interest using the CF96X Real Time System (BioRad, USA). The qPCR data were analyzed using the ΔΔCt method as described previously150 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 ml of supernatant was diluted with 3 ml of distilled H2O and applied to freshly prepared Dowex columns (AG1-X8; Bio-Rad). Columns were washed twice with distilled H2O ...
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bioRxiv - Developmental Biology 2022Quote: ... antibody-bead complexes were washed 3 times with lysis buffer before eluting the samples by boiling for 10 minutes in 4x loading dye (Bio-Rad) containing 10% β-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... Samples were checked for purity and presence of protein using 15% SDS-PAGE (Polyacrylamide gel electrophoresis, Mini Protean 3 System, Bio-Rad) and Coomassie Brilliant Blue (Merck ...
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bioRxiv - Microbiology 2020Quote: ... the capped full-length RNA and IBV-N transcripts dissolved in 400 μl PBS were co-transfected into Vero cells by electroporation (450 v, 50 μF, 3 mSec, GenePulser Xcell, BIO-RAD). After 48 h ...
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bioRxiv - Synthetic Biology 2021Quote: ... microwells containing assembled protocell arrays were all incubated at 37 °C for 3 hours before imaging on a ChemiDoc MP (Bio-Rad) imaging system ...
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bioRxiv - Immunology 2020Quote: ... the cells were divided into 100 μl aliquots and heated individually at different temperatures for 3 minutes (Thermal cycler, BIO-RAD) followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
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bioRxiv - Biochemistry 2021Quote: ... run with radioactive sample for 15 min at 200 V, dried (80°C, 3 hours) on a gel dryer (Bio-Rad), and exposed to a phosphor screen ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were incubated in the dark for 3 days at 30°C and then photographed in a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Beads were washed 3 times with ice cold nondenaturing lysis buffer and boiled in 4x Laemmli Sample Buffer (Bio-Rad, #1610747) for 10 mins ...
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bioRxiv - Immunology 2020Quote: ... 6µg of Round or Rod-shaped CCMVTT-VLPs were mixed with 2x mercaptoethanol and heated at 95°C for 3 minutes and then loaded into Any kD Mini-PROTEAN TGX precast protein gels (BIO-RAD). Gel was run for 35min at 180V ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated by 12 % SDS-PAGE using mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and 110 V for additional 2.5-3 h) and transferred to a nitrocellulose membrane overnight (~16 h) at 15 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5% fat-free milk in Tris buffered saline with Tween-20 (TBST ...
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bioRxiv - Zoology 2022Quote: ... sfRNA and 3’UTR were quantified together by RT-qPCR using the iTaq Universal Sybr green one-step kit (Bio-Rad) with primers previously designed [26] ...
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bioRxiv - Molecular Biology 2023Quote: ... LAMP reaction products were analysed on 2% agarose gels (65V for 3 hr) stained with ethidium bromide (10 mg/mL) and viewed on Gel Doc XR Imaging System (BioRad, USA).
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bioRxiv - Molecular Biology 2022Quote: ... The 10 µL cDNA reaction was diluted to 200 µL and 3 µL was used with iTaq Universal SYBR Green Supermix (Bio-Rad) and 670 nM of primer pairs (Table S4 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The relative amounts of the resulting products were analyzed by scanning the gels and determining the intensities of ethidium bromide stained bands using Image Lab software Version 1.36b (Bio-Rad) ...