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Citations for Bio-Rad :
7201 - 7250 of 9412 citations for Recombinant Human CPB1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred onto nitrocellulose membranes using Trans-Blot® TurboTM Transfer System RTA Transfer Kit (BioRad). Blotted membranes were blocked in 10% non-fat milk in PBS-Tween 20 0.05% for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Unconjugated dye was removed by passing the protein through Micro Bio-spin P-6 column (Bio-rad) equilibrated in GF150 buffer supplemented with 10% glycerol ...
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bioRxiv - Biochemistry 2024Quote: ... 30µg of protein was added per well and run on a 4-20% pre-cast gel (Biorad). Membranes were probed with α-ALDH2 (Proteintech 15310-1-AP ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate (20 µg) was separated on 4-20% Mini-PROTEAN® TGX Stain-FreeTM protein gels (Biorad) and transferred onto Immun-Blot® Low Fluorescence PVDF membrane (Biorad) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10-20 mg protein per lane was electrophoresed on 4-15% precast gradient gels (Bio-Rad). Based on the protein concentration and sample volumes ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF using either a Trans-Blot Turbo system (with Turbo transfer buffer) (BioRad) or by traditional wet tank transfer using CAPS Buffer with 10% Methanol (for DNA-PKcs) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to nitrocellulose membranes using a Trans-Blot® Turbo blot transfer pack (Bio-Rad). Nitrocellulose membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... T3SS effectors/proteins from supernatant were resuspended in Urea 8M and mixed with Laemmli buffer (Bio-Rad). OD600 of bacterial cultures was used to normalize the amount of sample for loading into the SDS-PAGE.
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bioRxiv - Neuroscience 2024Quote: ... then proteins were separated by SDS-PAGE and transferred to a PVDF membrane (BIO-RAD, 162-0177). The membrane was blocked with 5% skim milk in PBS containing 0.05% Tween 20 and then incubated with rabbit anti-RhoA (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane using the Trans-Blot Turbo transfer system (Bio-Rad). The membranes were blocked with 5% BSA in tris-buffered saline - 0.1% Tween (TBST ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 12% polyacrylamide gels and transferred to nitrocellulose membranes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Bait and prey proteins were loaded on separate gels (Bio-Rad 4-20% precast gels cat. #4568094). Proteins were transferred to nitrocellulose membrane and immunoblotted as described above ...
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bioRxiv - Plant Biology 2024Quote: ... The Supernatant was isolated and sample protein concentrations were calculated by Bradford assay (Bio-Rad Laboratories, 2010) using the Quick Start Bradford 1x Dye Reagent (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 20 μg of protein lysate was loaded onto an SDS-polyacrylamide gel and subjected to electrophoresis (BioRad). The proteins were then transferred from the gel onto a nitrocellulose membrane using a semi-dry method (BioRad Trans-Blot Turbo) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were run on 4-15% Mini-PROTEAN TGX precast protein gels (Bio-Rad Laboratories, Hercules, California) in running buffer (250 mM Tris ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The specific protein bands were visualized with a secondary antibody (anti-rabbit IgG-HRP conjugate; Bio-Rad) using a chemiluminescent substrate (Amersham ECL Western Blotting Detection Reagents ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.2 μm nitrocellulose membrane (Bio-Rad, Hercules, CA). After blocking with 5% skimmed milk ...
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bioRxiv - Microbiology 2020Quote: ... Levels of the following 27 cytokines were analyzed using a BioPlex Pro™ Human Cytokine 27-plex Assay kit (#M500KCAF0Y, Bio-Rad, Hercules, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... utilizing the bead-based immunoassay approach Bio-Plex Pro™ Human Cytokine 40-plex Assay (Cat# 171AK99MR2) on a Bio-plex 200 system (Cat# 171000201) (Bio-Rad Laboratories, Hercules, CA) and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30 ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... in oral swabs of positive and negative subjects for SARS-CoV-2 using magnetic bead-based multiplex immunoassays (Bio-Plex Pro™ human cytokine 27-plex panel, Bio-Rad Laboratories, Milan, Italy) according to the pre-optimized protocol 15 ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: Culture medium of HUVECs were collected and analyzed with the Bio-Plex Pro™ Human Cytokine 27-plex Panel (Bio-Rad Laboratories, Hercules, CA, USA) in accordance with the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 μg of denatured proteins were separated by SDS-PAGE (Mini-PROTEAN TGX Gels 10%, Bio-Rad Laboratories) and transferred to 0.45 μm nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Seventy-five micrograms of protein lysate were resolved on 4-12% TGX Acrylamide Stain-Free gels (Bio-Rad). Stain-Free gels were imaged prior to transfer to PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to nitrocellulose using a Trans-blot Turbo system (Bio-Rad). Protein concentrations were measured by Bradford assay using Protein Assay Dye Reagent Concentrate (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations in the cell lysates were estimated using the Quick Start™ Bradford Dye Reagent (Biorad# 5000205) followed by blocking of free thiol groups in HEN buffer containing 0.2% MMTS and 2.5% SDS at 50°C for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The cleared lysates were first run on precast protein gels (PN:456-1096 or 456-1093, Bio-Rad) in MOPS SDS running buffer (B0001 ...
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bioRxiv - Microbiology 2021Quote: ... 10-15 µg of protein lysate was resolved by SDS-PAGE on TGX Stain-Free acrylamide gels (BioRad). Membranes were blocked in 5% BSA or 5% skim milk in Tris buffered saline-Tween20 (TBS-T) ...
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bioRxiv - Genomics 2021Quote: ... The proteins were separated in the first dimension on 4-7 and 5-8 IPG strips (Bio-Rad). The strips were then loaded onto 8-20% gradient PAGE for separation on the second dimension ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentration was determined and 150 or 300 μg was loaded onto precast 4-15% TGX gels (BioRad) and blotted onto PVDF membranes for 1 hour at 100V at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and 20 μL diluted protein was added to each well of a 96-well PCR plate (Bio-Rad) in triplicate ...
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bioRxiv - Developmental Biology 2020Quote: ... Equal amounts of protein were loaded onto 10% or 4–15% Mini-PROTEAN TGX precast gels (Bio-Rad) and analyzed by Western blot ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein extract equivalent to 50 μg was loaded per lane onto precast gradient gels (4-20% Bio-rad). Separated proteins were then transferred to nitrocellulose membranes ...
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bioRxiv - Physiology 2022Quote: ... 15µl sample mix containing 12µg total protein and 4µl sample buffer (4× Laemmli Sample Buffer, Bio-Rad, 1610747) with 10% mercaptoethanol (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were subjected to SDS-β PAGE gel electrophoresis using pre-made gradient gels (4-20%, Biorad). Proteins were transferred by wet transfer to a PVDF membrane (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... 10-15 µg of protein lysate was resolved by SDS-PAGE on TGX Stain-Free acrylamide gels (BioRad). Total protein images were acquired from the PVDF membranes after transfer on the ChemiDoc Touch Imaging system (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Total protein images were acquired from the PVDF membranes after transfer on the ChemiDoc Touch Imaging system (BioRad). Membranes were blocked in 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: ... 10-15 µg of protein lysate was resolved by SDS-PAGE on TGX Stain-Free acrylamide gels (BioRad). Total protein images were acquired from the PVDF membranes after transfer on the ChemiDoc Touch Imaging system (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Total protein images were acquired from the PVDF membranes after transfer on the ChemiDoc Touch Imaging system (BioRad). Membranes were blocked in 5% BSA in TBS-T (Tris-buffered saline 0.1% Tween-20) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then measured to 20μg total protein in 2x Laemmli buffer containing 10% b-mercaptoethanol (Bio-rad), heated at 70°C for 10min and loaded into 4-20% precast gels (Bio-rad ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were prepared to a final concentration of 5 μg/μl in 4x Laemmli buffer (Bio-Rad) containing 25 mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... Calcium levels were normalized to protein levels measured using the Bradford assay (Bio-Rad Laboratories, Hercules, CA, # 5000006).
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bioRxiv - Molecular Biology 2020Quote: ... Viral proteins were separated on a Criterion™ TGX Strain-Free 4-15 % gradient gel (Biorad, CA, USA) (TGS ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... protein extracts were prepared in lysis buffer with 2% SDS and resolved in a 4-12% gel (Biorad). Membranes were probed overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein were separated on anykD® mini-PROTEAN TGX polyacrylamide gels (Bio-Rad, Hercules, CA) and transferred to an Immobilon-FL PVDF membrane (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred onto PVDF or nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) and transfer efficiency assessed by Ponceau Red staining ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant removed to a new microcentrifuge tube and protein concentration determined using the Bradford assay (Bio-Rad).
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bioRxiv - Biophysics 2022Quote: ... The cleared lysates were first run on precast protein gels (PN:456-1096 or 456-1093, Bio-Rad) in MOPS SDS running buffer (B0001 ...
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bioRxiv - Cell Biology 2022Quote: ... the protein signal on the membrane was recorded with a ChemiDoc XRS system (Bio-Rad Laboratories, Richmond, CA), and analyzed using the Image Lab software (Bio-Rad Laboratories).
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bioRxiv - Genomics 2020Quote: ... 15 µg of protein extract were loaded with 5x Laemmli buffer on Mini Protean TGX gels (Bio-Rad) and run at 100V for 1.5 h ...