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Citations for Bio-Rad :
7151 - 7200 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Protein lysates (40 μg per lane) were loaded on a 4-15% SDS-PAGE gel (Bio-Rad), separated in 1X Tris-Glycine Buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: 25µg of protein lysates were denatured at 95℃ for 5 minutes in 4x Laemmli Sample Buffer (BioRad) containing β-Mercaptoethanol (Millipore Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were then transferred to PVDF or Nitrocellulose membranes according to manufacturer’s instructions (Bio-Rad, Hercules). After 1 hour of blocking in 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... lysates or immunoprecipitated proteins were separated on SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad). The membranes were blocked with skim milk (5% ...
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bioRxiv - Pathology 2023Quote: ... protein bands were visualized using a chemiluminescence (ECL) detection system (Bio-Rad Laboratories Inc., Hercules, CA, USA). Adequate protein loading was confirmed by probing for GAPDH.
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bioRxiv - Genetics 2023Quote: Western blot – The different protein lysates were fractionated on 5-12% SDS-PAGE precast gels (Bio-Rad) and transferred onto Trans-Blot Turbo Mini 0.2 µm PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad). Immunoblotting and detection were performed on the PVDF membrane with SNAP i.d.® 2.0 Protein Detection System (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... An equal amount of protein was subjected to SDS-PAGE using TGX FastCast acrylamide gels (Bio-Rad) and transfer to nitrocellulose membrane (Trans-blot turbo transfer pack ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated protein was resolved by SDS-PAGE and transferred to polyvinylidene fluoride membrane (Bio-Rad, #162-0177). Western blot analysis was carried out as described (60 ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was transferred to fresh tubes and protein concentration was determined by Lowry assay (Bio-Rad, 5000113).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred from SDS-PAGE gels to nitrocellulose membranes using Trans-Blot Turbo (Bio-Rad Laboratories). Membrane was blocked in 5% milk or 5% BSA in TBS and incubated overnight at 4°C and for 1 hour at RT with primary and secondary HRP-conjugated antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... 20 μg protein per lane was run on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (BIO-RAD #1610158) at 40 mA for ∼1 h ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations from tissue lysates were determined using the Bradford assay kit (Biorad, USA; Cat# 5000006). Equal amounts of protein (30 μg ...
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bioRxiv - Microbiology 2023Quote: ... Staining of purified proteins after SDS Page was performed using Coomassie Brilliant Blue R-250 reagents (Biorad) and imaged on an Odyssey CLx infrared imaging system (Li-Cor Bioscience).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... proteins were transferred from the gel to a 0.2 µm polyvinyldenfluoride (PVDF) membrane (Transfer pack; Bio-Rad) using the TransBlot Turbo system (Bio-Rad ...
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (15–30 μg) were separated on 4–20% Tris Glycine SDS gels (BioRad) and transferred to polyvinylidene difluoride membranes (BioRad) ...
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bioRxiv - Genetics 2023Quote: Samples were electrophoresed on 4 to 15% Mini-PROTEAN TGX Stain-Free protein gels (Bio-Rad, #4568084) for 60 min at 100 V ...
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bioRxiv - Genetics 2023Quote: ... Low-level expressed gene ribosomal protein S15 (RPS15) was preamplified with SsoAdvanced PreAmp Supermix (#1725160, Bio-Rad), with 3 min at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... Resolved proteins were transferred to polyvinylidene fluoride (PVDF) membranes using the Trans-Blot Turbo Transfer System (BioRad), and the membrane was incubated with a 1:5000 dilution of an α-VSVG antibody ...
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bioRxiv - Genomics 2023Quote: ... Proteins were transferred onto an activated PVDF membrane using the Trans-Blot Turbo Transfer system (Bio-Rad). Membranes were blocked in 5% milk in PBS-T for 1h at room temperature before incubation with primary antibodies overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell debris was pelleted by microcentrifugation at 4°C and protein levels quantified using Bradford Assays (BioRad) as previously described (38) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS-PAGE on 4-15% gradient gels (Mini-PROTEAN Precast gels, Bio-Rad) in running buffer (200 mM glycine ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in 10% SDS-PAGE gels and transferred onto a 0.45µm nitrocellulose membrane (Bio-Rad). Membranes were blocked with 5% non-fat milk and incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Individual oxidized proteins were resolved by SDS-PAGE on 4-20% polyacrylamide gel gradient gel (BioRad, 5671093). Resolved proteins were transferred to PVDF membrane at 75 V for 2h ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were transferred to new tubes and total protein was quantified using a Bradford Assay (Bio-Rad). 20 µg protein per sample was reduced with 3 mM TCEP (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The purity and sequence of the tag-cleaved SLO protein were confirmed by SDS-PAGE (Bio-Rad) and bottom-up MS (BU-MS) ...
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bioRxiv - Bioengineering 2023Quote: ... protein transfer was performed viaTrans-Blot® Turbo™ RTA Mini PVDF Transfer Kit (Bio-Rad, USA). The membrane was blocked with PBS-T (0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Thirty micrograms of protein lysate were resolved on 4-12% TGX Acrylamide Stain-Free gels (Bio-Rad). Stain-Free gels were imaged prior to transfer to PVDF membrane (Millipore or Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on a precast Mini-PROTEAN® TGX™ 10% SDS-PAGE gel (4561034; BioRad) and then transferred to a 0.45 μm nitrocellulose membrane in Towbin SDS transfer buffer (25 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a PVDF-membrane by using the Trans Blot Turbo Transfer Pack (Bio-Rad). Membranes were incubated for 24 hours with blocking buffer (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... with ImageLab software (Image Lab v6, Bio- Rad) then proteins were transferred (Turbo Transfer system, Bio-Rad) to a methanol-saturated polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were separated using PAGE gel electrophoresis before being transferred to a 0.45µM nitrocellulose membrane (BioRad). Before probing with primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... All protein samples were separated by SDS-PAGE and transferred on to PVDF membranes (Bio-Rad #1704274). Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136 ...
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bioRxiv - Biochemistry 2023Quote: ... 3H-2DG uptake was subsequently adjusted for protein content using a detergent compatible (DC)-assay (Bio-Rad). The glucose-uptake experiment was repeated on three separate days with same observed effect ...
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bioRxiv - Cell Biology 2024Quote: ... 50-100mg of protein was loaded and resolved by SDS PAGE and transferred onto PVDF membrane (BioRad). The membrane was blocked using 5% BSA in TBST for 1 hour at room temperature and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... conjugated antibody was used to detect the desired protein by chemoluminescence with ECL (Bio-Rad, #170-5061).
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bioRxiv - Bioengineering 2024Quote: ... 30µg of protein was separated by electrophoresis through 7.5% polyacrylamide gels (Mini-PROTEAN, #456-8024, Bio-Rad) and transferred to nitrocellulose membranes (#1704159 ...
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bioRxiv - Neuroscience 2024Quote: ... Reagents for SDS-polyacrylamide gel electrophoresis and Bradford protein assays were from Bio-Rad (Hercules, CA, USA), fetal bovine serum and enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2024Quote: ... and mixed with a one-third volume of 4×Laemmli protein sample buffer (cat. # 1610747, Bio-Rad). Following centrifugation at 20,000×g at 4°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 10–20 μg of total protein was separated on 4–12% Bis-Tris gradient gels (Bio-Rad) by SDS-PAGE and then transferred to nitrocellulose membranes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins were transferred to a PVDF membrane using the Trans-Blot Turbo system (Bio-Rad Laboratories, USA). The membrane was washed with TBST solution and then blocked with Quick Blocking Buffer (NCM biotech ...
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bioRxiv - Molecular Biology 2024Quote: ... Thirty micrograms of protein lysate were resolved on 4-12% TGX Acrylamide Stain-Free gels (Bio-Rad). The Stain-Free gels were imaged prior to transfer to PVDF (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... and 15ug of each sample was loaded into a 4-20% SDS-PAGE precast protein gel (BioRad). Proteins were fractionated by electrophoresis and then transferred to PVDF membranes (BioRad) ...
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bioRxiv - Biophysics 2024Quote: ... 50 μg of protein was loaded in each well of a 4-20% precast polyacrylamide gel (BioRad). Gels were transferred (1.5 A for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were prepared for western blot analysis by adding 4x sample buffer (Bio-Rad, Cat. #1610747) and 1 mM 2-mercaptoethanol (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... 15 µg of protein sample was run on precast 4-15% gradient Tris-Glycine gels (BioRad 4561084) at 90V for 15 minutes and 120V for 60 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty µg of nuclear proteins were separated on precast 4-15% SDS-polyacrylamide gels (Bio-Rad Laboratories), and then transferred onto polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2024Quote: ... Band densities were normalized to the total amount of protein loaded per lane using Sypro Ruby (BioRad).