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Citations for Bio-Rad :
7151 - 7200 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and proteins were transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad), all according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were resolved on a 4-15% Mini-PROTEAN TGC precast polyacrylamide gel (Bio-Rad, #4561083EDU) in 1x Tris/Glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: Reactive proteins were revealed using chemiluminescent detection system (Clarity Western ECL Substrate, Bio-Rad, California, USA) and visualized on a Chemidoc Touch imaging system (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... The proteins were transferred to a PVDF membrane using a Trans-Blot-Turbo System (Bio-Rad) at a constant voltage of 25V and 2.5A current for 3min ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were mixed to load 10-20 μg protein/well with 4x Laemmli buffer (Biorad 1610747) according to manufacturer specifications ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to 0.2 µm nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad). Membranes were blocked for 1 hour in PBS-T with 5% skim milk powder ...
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bioRxiv - Cancer Biology 2024Quote: ... resolved proteins were transferred to nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad), then blocked with 4% BSA-TBS (Tris-buffered saline ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was separated by SDS-PAGE and transferred to PVDF membrane using the TransBlot Turbo (BioRad). Membranes were blocked in LI-COR Odyssey buffer (#927-50000 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) following the manufacturer’s instructions.Flag-GFP-tagged PimE was detected using a monoclonal ANTI-FLAG M2-Peroxidase (HRP ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). His-tagged PimE mutants were directly detected with HisProbe-Horseradish Peroxidase (HRP ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was separated by SDS-PAGE and transferred to PVDF membrane using the TransBlot Turbo (BioRad). Membranes were blocked with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Biochemistry 2024Quote: ... 20% methanol) and proteins were transferred to PVDF membrane using Trans-Blot SD transfer cell (Biorad). After transfer ...
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bioRxiv - Genetics 2024Quote: ... Inputs and bound fractions were resolved on a 4-20% gradient mini PROTEIN gel (Bio-Rad) and quantified by western blot probed with Avi Tag (1D11D10 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Proteins were blotted to Immobilon-PSQ (0.2 micron) PVDF membrane using the Criterion system (Bio-Rad). Membranes were stained with REVERT total protein stain (LI-COR Biosciences ...
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bioRxiv - Immunology 2023Quote: ... The second kit used was the Bio-Plex Pro Serology Reagent Kit (Bio-Rad, Cat No. 12014777), with human IgA positive and negative controls (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentration was assessed in cell culture supernatants 48 h post transfection by using immunoassays formatted on magnetic beads (Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y) (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol via Luminex 100 technology (Luminex ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of each of the human plasma samples from the small cohort were diluted in 17 µL of Laemmli sample buffer (Bio-Rad; Hercules, CA, USA) followed by denaturing at 70 °C for 10 min and the resulting denatured 20 µL samples were loaded onto 12% Bis-Tris PAGE gel (GenScript ...
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bioRxiv - Molecular Biology 2024Quote: ... using Bio-Rad Bio-Plex Human Cytokine Screening Panel Express and measured in the Bio-Rad Bio-Plex 200 System (Bio-Rad Laboratories, Hercules CA). Secreted IL-8 was measured from conditioned media using IL-8 DuoSet ELISA (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... or the RNeasy Micro Kit (Quiagen) and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad). Quantitative realtime PCR was performed using the GoTaq SYBR Green qPCR Master Mix (Promega ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was produced using a cDNA Synthesis kit (iScript™ cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA). The diluted cDNA was used for real-time PCR quantification of mRNA using iTaq SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... 500ng of RNA was reverse transcribed using the reverse transcription kit (iScript cDNA synthesis kit, Biorad, Cat# 1708891). The resultant cDNA was diluted ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blots were run on 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4561024) for 10 minutes at 90V and 45 minutes at 120V ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... 35 μg of protein lysate was loaded onto an SDS-PAGE gel (10% Pre-cast, Bio-Rad), ran for 1 h at 100 V and subsequently blotted onto a nitrocellulose membrane ...
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bioRxiv - Biophysics 2021Quote: ... Purity of proteins was determined by SDS-PAGE using 4–15% Mini-PROTEAN TGX gel (Bio-Rad). The C-terminal purification His-tag was not removed.
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bioRxiv - Bioengineering 2021Quote: ... the proteins produced were quantified by measuring the fluorescence or by Bradford assay (Bio-Rad, USA, 5000006).
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bioRxiv - Cancer Biology 2021Quote: ... Forty micrograms of protein lysate were resolved on 4-12% TGX Acrylamide Stain-Free gels (Bio-Rad). Proteins were transferred to PVDF membrane (Millipore) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 40μg of protein lysate per sample was loaded on 4-20% polyacrylamide gels (Bio-Rad Laboratories) for separation ...
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bioRxiv - Cell Biology 2020Quote: ... the seeking proteins were visualized using the enhanced chemiluminescent reagent (Beyotime) through an imaging system (Bio-Rad). The antibodies used were listed as follows ...
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bioRxiv - Cell Biology 2020Quote: ... IgG with SureBeads™ Protein G magnetic beads (J2112LB-02) were purchased from Bio-Rad (Hercules, CA). DAPI (D9542 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysate (30 μg protein) per well was loaded in a 12% Mini-PROTEAN TGX gel (BIO-RAD) and then transferred to a PVDF membrane (BIO-RAD).
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bioRxiv - Cell Biology 2020Quote: ... in 1x MOPS-SDS buffer and then transferring the proteins to 0.45 μm nitrocellulose membrane (BioRad, 1620115) for two hours at 4 °C in transfer buffer containing 20% methanol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein extracts collected after centrifugation were subjected to SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). After blocking with PBS containing 0.1% Tween-20 and 5% milk ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to unsupported nitrocellulose membranes in 1x TG (25mM Tris, 192mM glycine, pH8.3) (Bio-Rad) for 1.5 hours at 4C ...
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bioRxiv - Immunology 2021Quote: ... Total cell lysate was extracted using RIPA buffer and quantified using the DC protein assay (Bio-Rad). 15–30 μg of protein was loaded and run on 4%–12% SDS PAGE gels ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD) at 20 V for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein-Ni-NTA mixture was loaded on a Poly-Prep® Chromatography column (BioRad Laboratories, Munich) and washed with buffer (10 mM Tris/HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... boiled and loaded on a 12% Mini-PROTEAN® TGX™ Precast Protein Gel (BioRad Hercules, CA) run at 70 V for 2.5h ...
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bioRxiv - Biochemistry 2022Quote: ... The final purified protein preparation was analyzed by SDS-PAGE and stained with Coomassie Blue (Bio-Rad; 0.5% suspended in 40% Methanol ...
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bioRxiv - Cell Biology 2022Quote: ... Detection and quantification of target proteins was carried out on a scanning system (BIO-RAD, ChemiDocTM MP) using horseradish peroxidase (HRP)-conjugated secondary antibodies and ECL detection reagent ...
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bioRxiv - Genomics 2022Quote: ... Equal amounts of proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad #1704271), blocked in PBS with 5% (w/v ...
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bioRxiv - Genetics 2022Quote: ... salivary proteins were transferred to methanol treated PVDF membranes using the Trans-Blot Turbo Transfer system (BioRad). After transfer ...
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bioRxiv - Biochemistry 2022Quote: A sample of the purified protein was run on a Mini-PROTEAN TGX Stain-free gel (BIORAD) with 4-20% acrylamide gradient and stained with Coomassie brilliant blue ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were separated by SDS polyacrylamide gel electrophoresis and transferred to 0.2 µm nitrocellulose membrane (Bio-Rad). Protein transfer and equal loading were checked by staining with Ponceau S ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then transferred onto nitrocellulose (NC) membrane using an electrophoretic transfer cell (Bio-Rad Laboratories, USA) at RT (28) ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity was normalized to total protein concentration in the lysates measured via Bradford assay (Bio-Rad). See Supplementary Table S1 for additional information regarding experimental replicates.
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bioRxiv - Neuroscience 2021Quote: ... and 10-15 μg of protein were loaded onto 4–15% Criterion TGX gels (Bio-Rad: 5671084) in running buffer (3.03 g Tris base ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated by electrophoresis (SDS-PAGE) and transferred to ImmunoBlot polyvinylidene difluoride (PVDF) membranes (BioRad Laboratories). Western blot analyses were performed using primary antibodies recognizing ATM (D2E2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were separated using SDS-PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane (#88518, Biorad) The PVDF membrane was blocked with 5% Blotto (SC-2325 ...
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bioRxiv - Physiology 2021Quote: ... Sixty μg of protein were loaded per well in a 4-15% acrylamide TGX gel (Bio-Rad) and run in 1x Tris-Glycine SDS buffer ...