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Citations for Bio-Rad :
7051 - 7100 of 7957 citations for Myeloperoxidase MPO Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated with secondary goat-anti-rabbit IgG (Bio-Rad Laboratories, CA, USA; dilution 1:3000) or goat anti-mouse IgG (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... and appropriate volumes of the hepatocyte suspension were embedded in 1% agarose blocks using disposable plug molds (BioRad). Hepatocytes were thoroughly lysed in-agarose using 1% lithium dodecyl sulfate in notothenioid ringer (100 mM sodium phosphate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies were used: goat anti-rabbit IgG HRP conjugate (Bio-Rad, 170-6515, 1:5000) and goat anti-mouse IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were incubated in 10% EtOH for 1 hr before imaging fluorescence on a ChemiDoc instrument (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... which included 1 x QX200™ ddPCR™ Evagreen® Supermix (Bio-Rad, CA, USA; catalogue number 1864033), 100 nM of the forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... and transformed by electroporation using the Gene Pulser Xcell (1700 V, 25 µF, 200 Ω, 1 pulse, Biorad) in C2989 5alpha electrocompetent bacteria (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were run on a 1% agarose gel and imaged using a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: ... 20 mM imidazole and 1 mM DTT) and were then transferred to a disposable column (Bio-Rad, USA). Protein was eluted using the elution buffer (50 mM phosphate buffer pH 8 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
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bioRxiv - Immunology 2023Quote: ... by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf) on a real time thermal cycler CFX96 (Biorad).
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 h at room temperature with 5% non-fat dry milk (Bio-Rad Laboratories) in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... diluted 1:5000 in TBE for 30 minutes while shaking and imaged on Gel Doc XR+(Bio-Rad). Full-length bands for at each time point were calculated by densitometry using Image Lab software (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... Screens were analyzed using a Molecular Imager FX and the Quantity One 1-D Analysis Software (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reverse transcribed to cDNA ...
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bioRxiv - Microbiology 2024Quote: ... 10 or 100 ng of plasmid DNA was transferred to a 1 mm MicroPulser electroporation cuvette (Bio-Rad) and cells were then electroporated at 1.8 kV ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed thrice and incubated with appropriate secondary antibodies conjugated with HRP (Bio-rad; 1:3,000). Bands were detected by Western-Lightning Plus Enhanced Chemiluminescence (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2024Quote: ... The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reversibly transcribed to cDNA through reverse transcriptase PCR.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight with the following primary antibodies: αWIPI2 1:3000 (mouse, Bio-Rad, Hercules, CA, MCA5780GA), αWIPI4/WDR45 1:2000 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... HRP detection was performed by adding 1 ml Clarity Max™ Western ECL Blotting Substrates from Bio-Rad. Arrays were imaged using BioRad X-ray imager and quantified using ImageJ ...
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bioRxiv - Biochemistry 2024Quote: ... Normalised samples (1 – 3 µg) were electrophoresed on the 4-20% Criterion TGX Stain-Free Precast gels (BioRad) in Tris/Glycine/SDS running buffer (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked at room temperature for 1 hour in 5% nonfat dry milk (blocking grade, Bio-Rad) dissolved in Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Developmental Biology 2022Quote: ... Equal amounts of RNA for all samples in Fig 3N were simultaneously transcribed into cDNA using iScript cDNA synthesis kit (BIO-RAD, Hercules, CA, cat#170-8891) according to the manufacturer’s protocol within 7 days of the initial dissections to preserve sample quality ...
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bioRxiv - Cancer Biology 2020Quote: ... blots were developed using an ECL Ultra kit (New Cell & Molecular Biotech Co. Ltd, China) and images were captured using a ChemiDocTM XRS + Imager (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels of above-mentioned genes were estimated using iTaq™ Universal SYBR® Green One-step qRT-PCR kit (#1725151, Bio-Rad Laboratories Inc., USA) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... This sequence was amplified from B6J genomic DNA extracted from spleen tissue using iProof High-Fidelity PCR kit (BIO-RAD, Hercules, CA, USA; Cat# 172-5330) with the following primers containing XhoI and HindIII restriction enzyme sites in the sense and antisense primers respectively ...
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bioRxiv - Plant Biology 2019Quote: ... qrt-PCR reaction was prepared using 2x SensiMix™ SYBR & Fluorescein Kit (PEQLAB LLC, Wilmington, DE, USA) and PCR was conducted with a combination of iCycler and IQ5 (Bio-Rad Laboratories, Inc., Hercules, CA, USA). All transcript levels were normalized to the tubulin β gene (AT5G62690 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from the RNA according to the Bio-Rad iSCRIPT cDNA kit protocol using a Bio-Rad thermocycler (Bio-Rad Laboratories, Inc.; Hercules, CA, USA). After obtaining cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... The reverse transcription of the RNA was performed by using the iScript™ cDNA synthesis Kit (Cat. No. 1706691; Bio-Rad Laboratories AG, Hercules CA, USA). Relative mRNA expression levels were evaluated using the SYBR Green method ...
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bioRxiv - Cell Biology 2024Quote: ... RNA concentrations were measured by Nanodrop and 100ng of total RNA where subjected to first-strand cDNA synthesis with iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA, 170-8891). cDNA was amplified using the Luna Universal qPCR Master Mix (New England Biolabs M3003E ...
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bioRxiv - Biochemistry 2023Quote: ... following gel electrophoresis proteins were transferred from the gel to a polyvinylidene fluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad, Trans-Blot turbo RTA transfer kit) using the Bio-Rad standard protocol (25 V,30 min) ...
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bioRxiv - Biochemistry 2022Quote: ... and 1.0 μg of RNA per sample was reverse-transcribed was reverse transcribed using the iScript™ cDNA synthesis kit from Bio-Rad (Bio-Rad, Hercules, CA). Quantitative PCR was performed using SensiFAST SYBR Hi-ROX Kit in 25 µL reaction volume (Meridian Bioscience ...
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bioRxiv - Genomics 2023Quote: ... we used 1 μg of total RNA extracted (as above) from each of the 23 adults collected during 2022 for cDNA synthesis with iScript cDNA synthesis kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA). To screen for the Flavivirus Macrosiphum euphorbiae virus 1 (MeV-1) ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoblots were visualized using the Chemiluminescent HRP Substrate kit (MilliporeSigma, Burlington, MA, USA) and the ChemiDoc™ XRS+ Molecular Imager® (Bio-Rad Laboratories, Hercules, CA, USA). Densitometry quantification of immunoblot bands was performed using ImageJ.
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bioRxiv - Plant Biology 2020Quote: ... Real-time RT-PCR was performed using gene specific primers (Supplementary Table 1) and SYBR Green Supermix (Bio-Rad) in a CFX96 Real-Time PCR system (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... Immunostaining for the vitronectin receptor (VNR) was with anti-CD51/61 (clone 23C6, 1:400; Bio-Rad, Oxford, UK) and for cathepsin K with a rabbit polyclonal antibody (3368-100 ...
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bioRxiv - Cell Biology 2020Quote: All Rab34 used in steady-state assays was treated with AG 1-X8 anion exchange resin (Bio-Rad, 1401441) immediately before use to remove bound GDP ...
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bioRxiv - Genetics 2021Quote: ... Hydrolysis probe ddPCRs (20 μl volumes) consisted of 1 x ddPCR Supermix for probes without dUTP (Bio-Rad Laboratories), virus-specific primers (HHV-6B POL F and HHV-6B POL R ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker as follows: goat anti-mouse HRP conjugate (BioRad, 1706516; 1:5000), donkey anti-rabbit HRP conjugate (Amersham ...
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bioRxiv - Biophysics 2022Quote: ... Nanodisc reconstitution was induced by removing detergent with 0.8 mg ml-1 pre-washed Biobeads SM-2 (Bio-Rad) for 2 hours with gentle agitation at RT ...
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bioRxiv - Genetics 2022Quote: ... then 1-5ng added to a PCR mix containing 11 μl 2x ddPCR Supermix for Probe (no dUTP, BioRad), 1.1 μl 20x DMBT1 probe assay (FAM-labelled) ...
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bioRxiv - Molecular Biology 2020Quote: ... at 100 V for 1 hr in 4°C pre-chilled 1X Tris-Glycine buffer (Bio-Rad 161-0734). Membranes were blocked for 1 hr at room temperature in blocking buffer (PBS (Gibco 14190-144) ...
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bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the appropriate resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively with buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Plugs were loaded on 0.8% agarose gel in 1×TAE buffer (Certified Megabase agarose, Bio-Rad, ref# 161-3109) and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Transformation was done chemically with heat shock at 42 °C for 30 s or by electroporation with 0.1 cm gap cuvettes at 1.8 kV for 1 s (Gene Pulser, Bio-Rad), depending on the competent cells required ...
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bioRxiv - Microbiology 2019Quote: ... The integrity of the purified DNA template was assessed by conventional agarose gel (1% [wt/vol] electrophoresis (Bio-Rad), and the purified DNA samples were stored at 4 °C until further use.
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bioRxiv - Bioengineering 2020Quote: The cDNA was diluted 1/5 with dH2O and 2.5 µL were used for qPCR analysis (10 µL total volume; BioRad, iTaq Universal SYBR Green Supermix ...
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bioRxiv - Biochemistry 2020Quote: ... The reconstitution sample was nutated for 1 h at 4°C before addition of 0.2 g/mL SM2-BioBeads (BioRad), and the reconstitution sample was further nutated overnight at 4°C before removal of the biobeads ...
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bioRxiv - Microbiology 2019Quote: ... Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in approximately 10 μl M9 medium and spotted onto 1% low-fluorescence agarose pads (Bio-Rad) prepared in M9 medium and covered with a no1.5 glass coverslip ...