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Citations for Bio-Rad :
6951 - 7000 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on a precast Mini-PROTEAN® TGX™ 10% SDS-PAGE gel (4561034; BioRad) and then transferred to a 0.45 μm nitrocellulose membrane in Towbin SDS transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred from SDS-PAGE gels to nitrocellulose membranes using Trans-Blot Turbo (Bio-Rad Laboratories). Membrane was blocked in 5% milk or 5% BSA in TBS and incubated overnight at 4°C and for 1 hour at RT with primary and secondary HRP-conjugated antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated protein was resolved by SDS-PAGE and transferred to polyvinylidene fluoride membrane (Bio-Rad, #162-0177). Western blot analysis was carried out as described (60 ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was transferred to fresh tubes and protein concentration was determined by Lowry assay (Bio-Rad, 5000113).
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were then transferred to PVDF or Nitrocellulose membranes according to manufacturer’s instructions (Bio-Rad, Hercules). After 1 hour of blocking in 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Staining of purified proteins after SDS Page was performed using Coomassie Brilliant Blue R-250 reagents (Biorad) and imaged on an Odyssey CLx infrared imaging system (Li-Cor Bioscience).
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bioRxiv - Immunology 2023Quote: ... 10 μg of protein was run on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Bio-rad, 4561034) and transferred to a PVDF membrane (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were loaded onto a 4-15% Mini-PROTEAN TGX precast protein gel (Bio-Rad, cat.: 4561084). Following electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... and proteins were separated by SDS-PAGE on AnykD Criterion™ TGX™ precast gels (Bio-Rad) and transferred to 0.2 µm nitrocellulose membranes by using Trans-blot Turbo (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... 40 µg of protein extract was loaded on 10% polyacrylamide gels (TGX Stain-Free FastCast, Bio-Rad). Membranes were blocked with 5% BSA-TBST (5% BSA Sigma ...
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bioRxiv - Genetics 2023Quote: ... and Mbp1 proteins were all transferred onto 0.45 μm nitrocellulose membranes using a PROTEAN Tetra tank (BioRAD) filled with 25 mM Tris ...
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bioRxiv - Pathology 2023Quote: ... The separated proteins were transferred to a nitrocellulose membrane using the semi-dry transfer system (Bio-Rad). After blocking for one hour at room temperature with 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... All protein samples were separated by SDS-PAGE and transferred on to PVDF membranes (Bio-Rad #1704274). Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136 ...
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bioRxiv - Biochemistry 2023Quote: ... 3H-2DG uptake was subsequently adjusted for protein content using a detergent compatible (DC)-assay (Bio-Rad). The glucose-uptake experiment was repeated on three separate days with same observed effect ...
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bioRxiv - Developmental Biology 2023Quote: Protein (20 μg of each sample) was separated on 4-15% polyacrylamide gel (456-1083, Bio-Rad) and then transferred into a polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a PVDF membrane using a Mini Trans-Blot® Cell (Bio-Rad Laboratories) at 100V for 2h ...
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bioRxiv - Physiology 2023Quote: ... bursaria CCAP1660/18) were loaded on 10% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Biorad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transfer to a PVDF membrane using Trans-Blot Turbo Transfer system (Bio-Rad), blocked in 3% milk PBST and incubated overnight at 4°C with primary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were resolved by SDS-PAGE using Mini-Protean any kD or 4-20% gradient gels (BioRad) and transferred to nitrocellulose membrane (LI-COR) ...
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bioRxiv - Microbiology 2023Quote: ... The gel carrying the recombinant proteins and their derivatives was eventually stained with Coomassie Blue (Bio-Rad) for 1 hour and was de-stained with a de-staining solution (20% methanol and 10% acetic acid in water).
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bioRxiv - Cell Biology 2024Quote: ... 20 μg of protein lysate was loaded onto an SDS-polyacrylamide gel and subjected to electrophoresis (BioRad). The proteins were then transferred from the gel onto a nitrocellulose membrane using a semi-dry method (BioRad Trans-Blot Turbo) ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.2 μm nitrocellulose membrane (Bio-Rad, Hercules, CA). After blocking with 5% skimmed milk ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were run on 4-15% Mini-PROTEAN TGX precast protein gels (Bio-Rad Laboratories, Hercules, California) in running buffer (250 mM Tris ...
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bioRxiv - Plant Biology 2024Quote: ... The Supernatant was isolated and sample protein concentrations were calculated by Bradford assay (Bio-Rad Laboratories, 2010) using the Quick Start Bradford 1x Dye Reagent (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... 50 μg of protein was loaded in each well of a 4-20% precast polyacrylamide gel (BioRad). Gels were transferred (1.5 A for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... 15 µg of protein sample was run on precast 4-15% gradient Tris-Glycine gels (BioRad 4561084) at 90V for 15 minutes and 120V for 60 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 10–20 μg of total protein was separated on 4–12% Bis-Tris gradient gels (Bio-Rad) by SDS-PAGE and then transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2024Quote: ... 50-100mg of protein was loaded and resolved by SDS PAGE and transferred onto PVDF membrane (BioRad). The membrane was blocked using 5% BSA in TBST for 1 hour at room temperature and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Unconjugated dye was removed by passing the protein through Micro Bio-spin P-6 column (Bio-rad) equilibrated in GF150 buffer supplemented with 10% glycerol ...
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bioRxiv - Cancer Biology 2024Quote: ... conjugated antibody was used to detect the desired protein by chemoluminescence with ECL (Bio-Rad, #170-5061).
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bioRxiv - Bioengineering 2024Quote: ... 30µg of protein was separated by electrophoresis through 7.5% polyacrylamide gels (Mini-PROTEAN, #456-8024, Bio-Rad) and transferred to nitrocellulose membranes (#1704159 ...
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bioRxiv - Cell Biology 2024Quote: ... and mixed with a one-third volume of 4×Laemmli protein sample buffer (cat. # 1610747, Bio-Rad). Following centrifugation at 20,000×g at 4°C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... Reagents for SDS-polyacrylamide gel electrophoresis and Bradford protein assays were from Bio-Rad (Hercules, CA, USA), fetal bovine serum and enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were prepared for western blot analysis by adding 4x sample buffer (Bio-Rad, Cat. #1610747) and 1 mM 2-mercaptoethanol (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty µg of nuclear proteins were separated on precast 4-15% SDS-polyacrylamide gels (Bio-Rad Laboratories), and then transferred onto polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
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bioRxiv - Plant Biology 2024Quote: ... and 15ug of each sample was loaded into a 4-20% SDS-PAGE precast protein gel (BioRad). Proteins were fractionated by electrophoresis and then transferred to PVDF membranes (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Thirty micrograms of protein lysate were resolved on 4-12% TGX Acrylamide Stain-Free gels (Bio-Rad). The Stain-Free gels were imaged prior to transfer to PVDF (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Band densities were normalized to the total amount of protein loaded per lane using Sypro Ruby (BioRad).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins were transferred to a PVDF membrane using the Trans-Blot Turbo system (Bio-Rad Laboratories, USA). The membrane was washed with TBST solution and then blocked with Quick Blocking Buffer (NCM biotech ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane using the Trans-Blot Turbo transfer system (Bio-Rad). The membranes were blocked with 5% BSA in tris-buffered saline - 0.1% Tween (TBST ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 12% polyacrylamide gels and transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred onto nitrocellulose membranes using Trans-Blot® TurboTM Transfer System RTA Transfer Kit (BioRad). Blotted membranes were blocked in 10% non-fat milk in PBS-Tween 20 0.05% for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... 30µg of protein was added per well and run on a 4-20% pre-cast gel (Biorad). Membranes were probed with α-ALDH2 (Proteintech 15310-1-AP ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Bait and prey proteins were loaded on separate gels (Bio-Rad 4-20% precast gels cat. #4568094). Proteins were transferred to nitrocellulose membrane and immunoblotted as described above ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The specific protein bands were visualized with a secondary antibody (anti-rabbit IgG-HRP conjugate; Bio-Rad) using a chemiluminescent substrate (Amersham ECL Western Blotting Detection Reagents ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to nitrocellulose membranes using a Trans-Blot® Turbo blot transfer pack (Bio-Rad). Nitrocellulose membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF using either a Trans-Blot Turbo system (with Turbo transfer buffer) (BioRad) or by traditional wet tank transfer using CAPS Buffer with 10% Methanol (for DNA-PKcs) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate (20 µg) was separated on 4-20% Mini-PROTEAN® TGX Stain-FreeTM protein gels (Biorad) and transferred onto Immun-Blot® Low Fluorescence PVDF membrane (Biorad) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10-20 mg protein per lane was electrophoresed on 4-15% precast gradient gels (Bio-Rad). Based on the protein concentration and sample volumes ...
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bioRxiv - Microbiology 2024Quote: ... T3SS effectors/proteins from supernatant were resuspended in Urea 8M and mixed with Laemmli buffer (Bio-Rad). OD600 of bacterial cultures was used to normalize the amount of sample for loading into the SDS-PAGE.