Labshake search
Citations for Bio-Rad :
651 - 700 of 2617 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Cell concentration was determined for three independent cultures of each strain using the TC20 automated cell counter (Bio-Rad). These results were used to normalize the total areas of each chemical feature detected ...
-
bioRxiv - Neuroscience 2022Quote: ... the number of cells was counted using a Bio-Rad TC20 automated cell counter (Bio-Rad Laboratories, California, USA), using trypan blue (Cat#302643 ...
-
bioRxiv - Immunology 2022Quote: The viability of cells was assessed by trypan blue and counted in an automated TC20 cell counter (Bio-Rad) prior libraries preparation ...
-
bioRxiv - Systems Biology 2022Quote: ... The cells were sorted using the BD FACS Aria as single cells into a 96-well plate (Bio-Rad) containing 4μl lysis buffer (0.4% Triton X-100 in PBS ...
-
bioRxiv - Biochemistry 2022Quote: ... Cell number and viability were assessed by Trypan Blue (0.2%) staining using a TC10 automated cell counter (Bio-Rad Laboratories ...
-
bioRxiv - Immunology 2022Quote: ... The viability and concentration of cells after sort were measured using the TC20 Automated Cell Counter (#1450102, Bio-Rad). The viability of the cells pre-loading was > 90% ...
-
bioRxiv - Immunology 2023Quote: Equivalent number of T cells (1E6 cells/group) were lysed in Laemmli Sample Buffer with ²-mercaptoethanol (Bio-Rad, CA). Total cell lysate for each sample were resolved on Bis-Tris 4–12% gel (ThermoFisher NuPAGE ...
-
bioRxiv - Bioengineering 2023Quote: ... Old + siPiezo1) were harvested by enzymatic dissociation and cell viability was confirmed using an automated cell counter (Biorad, TC20). Cell hashing with anti-mouse oligo-tagged antibodies (TotalSeq A ...
-
bioRxiv - Cell Biology 2023Quote: ... For single cell RNA-sequencing cells were sorted with an 85um nozzle into 96-well PCR plates (Bio-Rad) containing 2ul of lysis buffer [0.2% Triton X-100 ...
-
bioRxiv - Biochemistry 2023Quote: ... The median fluorescence intensity of living single cells was determined in triplicates using a ZE5 Cell Analyzer (Bio-Rad) and FCS Express (De Novo Software ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were first embedded in gel plugs with approximately 1 million cells per gel plug (BioRad no. 170-3592). The high molecular weight genomic DNA was extracted and purified using the Bionano Genomics SP kit.
-
bioRxiv - Biophysics 2021Quote: ... and total cell counts in BALF were determined using the TC20 automated cell counter (Bio-Rad Laboratories, Inc., Hercules, CA). The differential cell counts were performed on cells cytocentrifuged onto glass slides (Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... GFP positive cells were single cell sorted using 85 micron nozzle in a FACSAriaIII machine into 96 well plates (Biorad) containing 30µl lysis buffer (2.5µl 0.1M Dithiothreitol ...
-
bioRxiv - Immunology 2022Quote: Cell viability was determined by 0.4% Trypan Blue dye exclusion test executed by a TC20 Automated Cell Counter (Bio-Rad).
-
bioRxiv - Genomics 2020Quote: ... was added to the cell pellet and the cell mixture was transferred to a 96-well PCR plate (Bio-Rad). The lysate was heated for 15 minutes at 65°C followed by 10 minutes at 98°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cell number was determined using trypan blue exclusion and quantification in a cell counter (TC-20, Bio-Rad, Vienna, Austria).
-
bioRxiv - Cancer Biology 2019Quote: 150,000 cells were seeded per T25 flask in mNSC media and counted after 72 hours on a TC20 cell counter (Biorad) using Trypan blue stain (Thermo) ...
-
bioRxiv - Bioengineering 2021Quote: ... The cell pellet was re-suspended in 10mL of fresh culture media (37°C) and cells density was calculated with a TC20 automated cell counter (BioRad). 100’000 to 150’000 cells were plated per 35mm glass-bottom dish and transfected the following day ...
-
bioRxiv - Cancer Biology 2021Quote: The cells were counted and examined for viability with trypan blue staining using a TC-20 cell counter (Bio-Rad). Approximately 10,000 live cells with above 70% viability were loaded into the Single Cell Chip to generate single cell emulsion in Chromium Controller (10x Genomics ...
-
bioRxiv - Biochemistry 2022Quote: ... The percentage of live cells was determined using trypan blue staining quantified using a TC10 Automated Cell Counter (Bio-Rad). To determine intracellular NADPH and oxidized glutathione (GSSG) ...
-
bioRxiv - Cell Biology 2022Quote: siRNAs-treated HeLa cells and Control KO and Cavin1 KO cells were directly lysed in 1x Laemmli (Bio-Rad Laboratories) with Benzonase nuclease (#70664 Millipore ...
-
bioRxiv - Developmental Biology 2022Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and the pellet was resuspended in 1 ml of BM1* and cells were counted using a TC20 Automated Cell Counter (BioRad). Single-cell suspensions were mostly re-seeded at the same density as their parental/previous generation/passage.
-
bioRxiv - Immunology 2020Quote: ... Cells were diluted in mock cells (electroporated without gRNA and then transduced) if needed and counted in a TC-20 automatic cell counter (Biorad). For immunizations ...
-
bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cell viability (Trypan blue exclusion) was confirmed to be greater than 90% using the TC20 Automated Cell Counter (Bio-Rad). Cell pellets were washed twice in PBS containing 0.04% BSA ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were trypsinized with 0.25% trypsin-EDTA and cells in suspension were counted using an automated cell counter (TC20, Bio-Rad) and resuspended in medium to a density of 5×105 cells/ml ...
-
bioRxiv - Genetics 2020Quote: ... LSK were plated at day 8 of culture and monitored for growth by counting live cells by Trypan blue exclusion using a TC20TM Automated Cell Couter (BIORAD) at the indicated time ...
-
bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 500 μl buffer and counted with trypan blue in a TC20 automated cell counter (BioRad). Cells were stained in the presence of a Fcγ-receptor blocker ...
-
bioRxiv - Cell Biology 2019Quote: For detecting phosphorylation of ERK1/2 in both HEK-293 cells and knockdown cells Western blotting analysis of the whole-cell lysate was performed and transferred to polyvinylidene difluoride membranes (PVDF;BioRad). The membrane was blocked with 5% BSA (SRL ...
-
bioRxiv - Molecular Biology 2019Quote: ... The percentage of live cells was determined every day by trypan blue exclusion using a TC20 Automated Cell Counter (BioRad). Cell density was maintained at 1.0×106 cells/mL.
-
bioRxiv - Cell Biology 2021Quote: ... Cell counting and viability assays were performed with trypan blue staining on an automated cell counter (Bio-Rad TC-20). Cells were cultured for indicated times after drug treatment and harvested as indicated for various assays.
-
bioRxiv - Cell Biology 2020Quote: ... The amount of ATP for one cell was derived from the amount of protein in one cell found via Bradford assay (BioRad).
-
bioRxiv - Cancer Biology 2020Quote: ... Around 40 μg of whole-cell lysate was resolved in 10% SDS-PAGE gel (Mini protein Tetra cell Bio-Rad) and blotted to nitrocellulose membrane (Bio-Rad ...
-
bioRxiv - Immunology 2021Quote: ... An aliquot of cells was stained with trypan blue and counted using Automated Cell Counter (Bio-Rad, Hercules, California, USA). Isolated PBMCs were then cryopreserved in a cryovial in cell recovery freezing medium containing 10% Dimethyl Sulfoxide (DMSO ...
-
bioRxiv - Immunology 2021Quote: ... Viable and dead lung cell counts were determined using 0.4% Trypan Blue solution and a TC10™ Automated Cell Counter (BioRad). BAL samples were centrifuged and supernatants from the first BAL retrieval harvested ...
-
bioRxiv - Biochemistry 2022Quote: ... Whole-cell extracts were prepared from cells grown in YPD and analyzed by SDS-PAGE using stain-free precast gels (Biorad) followed by westernblotting with monoclonal anti-GFP antibodies (clones 7.1 & 13.1 ...
-
bioRxiv - Cancer Biology 2022Quote: The isolated sEVs and m/lEVs from 1.5 × 106 cells following 72 h of cell culture were lysed with 4X sample buffer (Bio-Rad) with β-mercaptoethanol (for detecting TSG101 and flotillin-1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were resuspended in 1 mL of flow sorting buffer and viable cells were counted using trypan blue and the BioRad TC20 Automated Cell Counter (BioRad). Cells were pelleted and resuspended in 100 uL of flow sorting buffer and stained for 20 minutes in the dark with 1.25 uL of EpCAM-PE (CD326 ...
-
bioRxiv - Neuroscience 2022Quote: ... After resuspension in TeSR/E8 medium supplemented with 5 μM ROCK inhibitor cells were counted with a TC20 automated cell counter (Biorad) and resuspended to get a final concentration of 2 x 10^5 cells/ml ...
-
bioRxiv - Microbiology 2023Quote: ... At least 10,000 stained cells and viral particles were counted using an S3e Cell Sorter (Bio-Rad, Hercules, CA, USA) and analyzed using FlowJo (Becton ...
-
bioRxiv - Microbiology 2023Quote: ... Cell number and viability was determined via trypan blue exclusion assays using the Biorad TC20 automated cell counter (Biorad # 1450102). Briefly ...
-
bioRxiv - Cancer Biology 2023Quote: ... KM12 or HCT116 cells (10 million) along with the spike-in control (20%) were carefully resuspended in cell suspension buffer (BioRad). The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were resuspended in 500 μl ice cold PBS and examined on the S3e Cell Sorter (Bio-Rad Laboratories). The data of cell cycle profiles was analyzed using FlowJo software (BD Biosciences).
-
bioRxiv - Genomics 2023Quote: Permeabilized nuclei (see section Cell harvesting, fixation and permeabilization) were counted on a TC20™ Automated Cell Counter (BioRad, 1450102). Nuclei were diluted to ~2.5 x 106 cells/mL in Wash buffer 1 ...
-
bioRxiv - Immunology 2023Quote: ... Resulting cell suspensions were filtered (< 40 μm) and cell counts were obtained after trypan blue staining using an automated cell counter (BioRad).
-
bioRxiv - Neuroscience 2023Quote: ... Microglia were counted using trypan blue (live-dead assay) on an automated cell counter (TC20 Automated Cell counter, Biorad, 508BR08836).
-
bioRxiv - Bioengineering 2023Quote: ... The cells were resuspended with 200 µL of PBSB and analyzed on a flow cytometer (Bio-Rad, ZE5 cell analyzer).
-
bioRxiv - Bioengineering 2023Quote: ... The cells were resuspended with 200 μL of PBSB and analyzed on a flow cytometer (Bio-Rad, ZE5 cell analyzer).
-
bioRxiv - Cell Biology 2023Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Protein samples were loaded on 4-15% polyacrylamide gels (BioRad ...