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Citations for Bio-Rad :
651 - 700 of 4235 citations for Mouse IgG2b Isotype Control Antibody Biotin A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... followed by secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit immunoglobulin antibodies and chemiluminescence Clarity or Clarity max substrate (Bio-Rad). A Bio-Rad ChemiDoc imager was used.
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation for 2 hours at room temperature with HRP-conjugated goat anti-rabbit or goat anti-mouse secondary antibodies (Bio-Rad), diluted 1:5000 in 5% nonfat milk in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... were used for Western blotting together with anti-mouse IgG antibody conjugated with horseradish peroxidase (goat-polyclonal, 5178-2504, Bio-Rad). Chemicals were purchased from Sigma-Aldrich unless specified otherwise.
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bioRxiv - Molecular Biology 2019Quote: ... were purchased from Thermo Fisher Scientific while secondary antibodies Goat anti-mouse HRP (172-1011) and Goat anti-rabbit HRP (170-8241) were from Bio-Rad. MG132 (C2211 ...
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bioRxiv - Biochemistry 2020Quote: ... for 1hr at RT and probed with HRP-conjugated anti-rabbit or mouse IgG antibodies and detected with ChemiDoc XRS+ imaging system (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... the membrane was incubated with an anti-rabbit or anti-mouse IgG antibody conjugated with horse radish peroxidase (HRP) (BioRad Laboratories) and developed with Clarity Western ECL Substrate (BioRad Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies HRP-conjugated for mouse (CST 7076) or rabbit (CST 7074) were used with Clarity-ECL chemiluminescence reagent (Bio-Rad) for detection ...
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bioRxiv - Immunology 2022Quote: ... were coated with 50 µL/well of 2 µg/mL of mouse anti-bovine IFN-γ mAb (CC330 Bio-Rad Antibodies) diluted in carbonate coating buffer (pH 9.6 ...
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bioRxiv - Immunology 2023Quote: ... Bovine CD4+ T cells were isolated from PBMCs by magnetic-activated cell sorting (MACS) using a mouse anti-bovine CD4 antibody (clone CC8, Bio-Rad), anti-mouse IgG MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti mouse #1721011; Bio-Rad or goat anti rabbit #1706515; BioRad) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Peroxidase-conjugated goat anti-mouse (170-6516) and peroxidase-conjugated goat anti-rabbit (1706515) secondary antibodies were purchased from Bio-Rad.
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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bioRxiv - Physiology 2023Quote: ... The secondary antibodies were goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP conjugate (Bio-Rad, Hercules, CA, USA) for western blotting ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBS + 0.05% Tween-20 (PBS-T) and incubated in goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-rad) diluted 1:5000 in blocking solution ...
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bioRxiv - Immunology 2020Quote: ... Excess biotin was removed via size exclusion chromatography using an ENrich SEC 650 10 × 300 mm column (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... including CD44-bio (Miltenyi Cat#130-113-340) and an in-house biotin conjugation (Bio-Rad Laboratories, Cat#LNK262B) of anti-PSGL-1 and mouse IgG1 isotype control (R&D Cat#MAB002 ...
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bioRxiv - Cell Biology 2020Quote: ... Control scrambled siRNA or TRAF6 siRNA (30 nM) were transfected using siLentFect (Bio-Rad).
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bioRxiv - Developmental Biology 2021Quote: ... Ratios between test and internal control amplicons was determined in QuantaSoft software (Bio-Rad). Ratios were normalized to the mean ratio of three test amplicons located on different non-targeted chromosomes (1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Input controls were also saved and prepared with 4X Laemmli Sample Buffer (Bio-Rad) accordingly ...
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bioRxiv - Microbiology 2022Quote: ... Duplicate aliquots were incubated on ice (control) or in a thermocycler (BIO-RAD T100) at temperatures between 37 °C and 60 °C for 10 minutes.
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bioRxiv - Microbiology 2022Quote: ... Duplicate aliquots were incubated on ice (control) or in a thermocycler (BIO-RAD T100) at temperatures between 20 °C and 60 °C for 10 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... and β-actin as a control and analyzed on an IQ5 Thermal Cycler (BioRad) using the iQ SYBR Green Supermix (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Total proteins were visualized as internal control using stain free reagent in ChemiDoc (BioRad) with a 5 min exposition time and then transferred on 0.22 µm PVDF membrane (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... and 1: 25000 diluted secondary HRP conjugated Goat anti-mouse IgG (H+L) (BioRad, Mississauga, Canada). The blot was developed using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Microbiology 2023Quote: ... followed by 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 3% milk in PBS-T and developed as described above.
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were goat anti-rabbit HRP (1:5000, Bio-Rad: 170-5046) and goat anti-mouse HRP (1:5000 ...
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bioRxiv - Bioengineering 2019Quote: ... supernatants were incubated (1 h) with antibody-bound MagPlex microspheres (BioRad, Hercules, CA), succeeded by an incubation step with biotinylated antibodies (1 h) ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated in goat anti-rabbit IgG horseradish peroxidase secondary antibody (1:5000, BioRad) for 2 hours at room temperature and then washed again with TBST prior to imaging on a BioRad ChemiDoc XRS+ imaging system using enhanced chemiluminescence (Pierce Supersignal West Pico) ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated with the primary antibodies (1:100 dilution; CD68, Bio-Rad, Cat# MCA1957 ...
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bioRxiv - Cancer Biology 2020Quote: ... and a secondary antibody (goat anti-rabbit HRP, Bio-Rad, AB_11125142; 1:2,000) were used in these experiments.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A solution of rabbit αFITC antibody (1 μg/mL, Bio-Rad 4510-7804) in 2% Milk-PBS was added to each well (100 μL/well ...
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bioRxiv - Neuroscience 2022Quote: ... Secondaries for immunoblots were HRP-conjugated rabbit antibodies (Bio-Rad Laboratories, 1:10000).
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were used at 1:10000 dilution and were purchased from Biorad. Signal was visualized by the ECL solution (Biorad ...
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bioRxiv - Genetics 2022Quote: ... followed by an HRP-conjugated secondary antibody (Bio-Rad 1706515; 1:3000 dilution) before applying ECL HRP substrate (Bio-Rad 1705060 ...
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bioRxiv - Pathology 2022Quote: ... and then stained with primary antibodies against F4/80 (Cl:A3-1, Bio-Rad) and Ly6G or CD3ε (#87048S and #99940 ...
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bioRxiv - Neuroscience 2022Quote: ... blots were washed with TBS-T and secondary antibodies (1:5000; Bio-Rad) were applied for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... lung sections were stained with anti-ECFP antibody (1:100, #AHP2986, Bio-Rad) and Alexa Fluor 488 goat anti-rabbit secondary antibody (1:1500 ...
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bioRxiv - Cell Biology 2020Quote: ... the process was repeated with an alpha-tubulin antibody (BioRad MCA78G, 1:2000) to minimize cross-reaction ...
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bioRxiv - Neuroscience 2021Quote: ... Bands were visualized with secondary antibodies conjugated to HRP (1:10,000; Bio-Rad) and SuperSignal chemiluminescent substrates signal (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia was stained with rat monoclonal anti-CD68 antibody (1:100, Bio-Rad) and with a rabbit monoclonal anti-Iba1 antibody (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated in primary antibody (rat anti-MBP, BioRad, 1:200 dilution) for 24 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... they were incubated with secondary antibodies (dilution 1:500) coupled with HRP (BioRad, anti-mouse-HRP #1706516 and anti-rabbit-HRP #1706515) ...
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bioRxiv - Microbiology 2023Quote: ... and antibody detected with goat-anti-rabbit HRP (Bio-Rad, 1706515, 1:5000).
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bioRxiv - Plant Biology 2023Quote: ... with goat anti-rabbit HRP secondary antibody (1:3000, Bio-Rad #170-5046). Membranes were developed using the SuperSignal West Pico Chemiluminescent Substrate kit (Fisher #PI34578 ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary antibodies used were either anti-rabbit (170-6515 Bio-Rad; 1:3000) or ECL anti-mouse (NA931 Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... or rat monoclonal anti-CD11b antibody (1:1000; BioRad, catalogue# MCA711G, lot# 0614) in blocking buffer overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... HRP (horseradish peroxidase)-conjugated goat anti-mouse (#176516, 1:10,000 for WB) and anti-rabbit IgG antibodies (#176515, 1:10,000 for WB) were from Bio-Rad. Alexa Fluor conjugated goat anti-mouse (1:500 for IF) ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed three times with TBST and then incubated for 60 min at room temperature with horseradish peroxidase-conjugated secondary antibodies (1:3,000 dilution, ref: 172-1011 and 1:5,000 dilution, ref: 170-6515, Bio-Rad) in blocking buffer ...