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Citations for Bio-Rad :
6901 - 6950 of 7899 citations for 6 Quinoxalinecarboxaldehyde 1 2 3 4 tetrahydro 1 methyl 3 oxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of each sample was determined as described before and a total amount of 20 μg protein/sample was resolved on 4-20% gradient gels (Bio-Rad). Proteins were transferred onto nitrocellulose or PVDF membranes futher blocked with 5% non-fat dry milk-TBST ...
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bioRxiv - Microbiology 2022Quote: ... Equivalent protein amounts (EVs and proteins) were separated on 4-15% stain-free pre-cast SDS-PAGE gradient gels (Bio-Rad) under non-reducing conditions and transferred onto PVDF membranes (Sigma Aldrich) ...
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bioRxiv - Genetics 2022Quote: ... Protein samples were heated at 37°C for 5 min and 10 μl was loaded on SDS–polyacrylamide gel electrophoresis (PAGE) gels (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad). After electrophoresis for 30 min at 200V ...
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bioRxiv - Genetics 2022Quote: ... total proteins were visualized by in-gel fluorescence using a trihalo compound incorporated in SDS–PAGE gels (stain-free TGX gels, 4–20%; Bio-Rad) after 45 sec UV-induced photoactivation using a ChemiDoc MP imager (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... 5 µL of the soluble fractions of cell lysates were run on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad) and stained using Gelcode Blue (Thermo Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... Digestion was confirmed by running 5 µL of digested or diluted samples on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad), followed by staining with Gelcode Blue (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 μL sample was run on an SDS-PAGE gel (Mini-PROTEAN TGX, 4-15%, Bio-Rad Laboratories, Inc., Hercules, CA) prior to transfer to a PDVF membrane (Immobilon-P Membrane ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 14000 rpm for 20 min at 4°C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). Fifty micrograms of total extract were then loaded in SDS-polyacrylamide gels and transferred onto nitrocellulose membranes for western blot analysis ...
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bioRxiv - Genetics 2022Quote: ... and 25µg of protein was loaded in each well and resolved on 4-15% gradient Tris-Glycine gel (Bio-Rad - 4561086). Semi-dry transfer was done onto 0.2µm Nitrocellulose membrane as per Trans-BlotTurbo Kit (Bio-Rad - 1704270 ...
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bioRxiv - Genetics 2022Quote: ... or 25 µg (mouse tissues) of proteins were loaded per lane on Any kD or 4-20% Criterion TGX stain-free precast gels (Bio-Rad) and transferred to Immobilon-FL PVDF membrane (Millipore ...
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bioRxiv - Genetics 2022Quote: ... 5% of the resulting lysate from the nuclear and cytoplasmic fractions were resolved by SDS-PAGE gel electrophoresis using 4-15% gradient gels (Bio-rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on 10% or 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were boiled for 5 min at 95°C before separation on a gradient 4 to 20% sodium dodecyl sulfate (SDS)-polyacrylamide gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semidry transfer (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... All radioactive protein samples were separated by size on a 4-to-20% Mini-Protean TGX precast SDS-PAGE gel (Bio-Rad). The gel was then dried and imaged using a Typhoon phosphorimager (Molecular Dynamics GE Healthcare Life Sciences) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Obtained soluble and insoluble fraction (pellet of cell debris) was analyzed by SDS-PAGE using 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and Precision Plus Protein Dual Color Standard (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
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bioRxiv - Neuroscience 2022Quote: Protein samples and dual plus molecular weight ladders were separated by SDS-PAGE and loaded on a Precast stain free gels with a 4–15% gradient (Bio-Rad). Proteins were transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples (25 µg) were separated by electrophoresis on 4-15% mini-PROTEAN TGX precast gels (Bio-Rad, cat# 456-1083) and transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... diluted with 5X Laemmli buffer and boiled for 5 minutes at 95°C before being subjected to SDS-polyacrylamide gel electrophoresis using 15% (made in-house) or 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Physiology 2022Quote: ... Lysates were diluted with 4X Laemmli buffer containing beta-mercaptoethanol and boiled for 10 minutes at 95°C and subjected to SDS-polyacrylamide gel electrophoresis 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Microbiology 2022Quote: ... 30 μg of whole-cell lysate or IP elutions were resolved on 4 to 15% mini-PROTEAN TGX gels (Bio-Rad). Transfers to PVDF membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 10 min and loaded into a 4-15% gradient Tris-glycine gel (Biorad 4561084) immersed in Tris-glycine-SDS running buffer (Biorad 1610772) ...
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bioRxiv - Immunology 2022Quote: ... Even amounts of each protein sample were separated on a 4–16% Mini-PROTEAN TGX Stain-Free Precast Gels using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
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bioRxiv - Systems Biology 2022Quote: ... Equal amounts of each protein sample were separated on 4% to 16% Mini-Protean TGX stain-free precast gels using the Mini-Protean Tetra cell electrophoresis system (Bio-Rad). Upon the completion of electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: ... The same amount of proteins for each lysate was then separated by SDS-PAGE on 4-15 % precast gels (Bio-Rad) under reducing conditions ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were mixed with reducing SDS sample buffer and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4–15 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Western Blot analysis was performed as described previously (15).
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bioRxiv - Biochemistry 2024Quote: ... Lysates were cleared by centrifugation (10 min at 14,000 x g, 4°C) and quantified by Bradford (Bio-Rad, cat#5000205). All samples were then boiled for 5 min in Laemmli sample buffer ...
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bioRxiv - Biophysics 2024Quote: SDS-Page was performed with a 4%-12% gradient acrylamide Bis-tris gel with XT MES running buffer (Bio-Rad Laboratories). Both constructs nsp7-11C and nsp7-11N were mixed at 36 µM with 14 µM Mpro and incubated at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 15 µg of nuclear extract in SDS loading buffer were loaded on a 4-15% precast polyacrylamide gel (Bio-Rad #4561083EDU) and Color-coded Prestained Protein Marker ...
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bioRxiv - Biochemistry 2024Quote: ... the beads were mixed with 1.5 × loading buffer and then loaded on a 4-15% pre-casted protein gels (BioRad; #456-1084) prior to transfer on PVDF membrane for western blotting using anti-Flag ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on 10% or 4-20% CRITERION TGX gels under reducing conditions and transferred to Immun-Blot PVDF membranes (Bio-Rad). The membranes were blocked with 5% BSA or 5% nonfat milk in TBS-T (0.1% Tween20 ...
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bioRxiv - Molecular Biology 2024Quote: Samples in 1X Laemmli loading buffer (generally representing 1-2 µL of replication reaction or 9 µL of a plasmid pulldown sample) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to polyvinyl difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysates were separated by Bis-Tris sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4–20% precast polyacrylamide gel (BioRad, #4561096). The proteins were transferred to low fluorescence polyvinylidene difluoride (PVDF ...
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bioRxiv - Synthetic Biology 2024Quote: ... the maximum amount of protein that could be loaded to keep protein mass constant across all samples on a gel) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Physiology 2024Quote: ... Protein obtained was run on SDS-PAGE on precast polyacrylamide gel (Mini-PROTEAN TGX, 4%-20%; Bio-Rad Laboratories, Hercules, CA) and transferred to a polyvinylidene difluoride membrane via a semi-wet transfer equipment (Trans-Blot Turbo Transfer System ...
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bioRxiv - Neuroscience 2024Quote: ... They were resolved by SDS–PAGE using a Bio-rad system and 4–15% Mini-PROTEAN TGX Precast Protein Gels (#4561086, Bio-rad) and transferred to nitrocellulose membranes (pore size 0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 min and run on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad). Resolved protein was transferred to nitrocellulose using Trans-Blot turbo transfer packs ...
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bioRxiv - Microbiology 2024Quote: ... 20µg of protein samples were heated in 1X Laemmli buffer at 95°C for 5 min and loaded on pre-cast 4-20% SDS-polyacrylamide electrophoresis gel (Bio-Rad). For the RIP experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were heated at 95°C for 5 minutes and then separated on a SDS-PAGE on TGX-stain free precast gel (4-15% of acrylamide gradient) and transferred on a nitrocellulose filter using a semi-dry transfer apparatus (TransBlot SD; Bio-Rad). Then ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled for 10 minutes at 95°C before separation on a gradient of 4-20% SDS-PAGE gels (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for either semi-dry or wet transfer (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: Samples were prepared according to the western blot protocol and run through a 4-15% Mini-Protean Precast TGX Gel (Bio-Rad). The gels were then stained using the Pierce Silver Stain Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Samples were incubated at 95°C for 10 minutes and loaded onto SDS-polyacrylamide gels (Mini-Protean TGX gels 4-20%, Bio-Rad). Following gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and 30 µg of total protein extract were loaded on 10% acrylamide-bis-acrylamide gels or 4-20% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Proteins were separated by migration at 135 V in 1X Tris-Glycine-SDS buffer (Euromedex) ...
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bioRxiv - Molecular Biology 2023Quote: ... resin with immunoprecipitated proteins were resuspended in SDS-loading buffer and electrophoresed on a 4-20% TGX stain-free gel (Bio-Rad) for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... For each protein of interest 10 µg of sample was loaded in a 4-15% Tris-Glycine gel (Bio-Rad, 4568083). The gel was transfered onto PVDF membrane (GenScript ...
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bioRxiv - Biophysics 2024Quote: ... Econo-Pac 10DG Desalting Columns and 4-20% gradient Mini-PROTEAN®TGX™ Precast Gels were purchased from Bio-Rad Laboratories (USA) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extractions containing 5 µg of Chl with 1 x SDS loading buffer were boiled at 100℃ for 5 min and loaded onto a 4 - 20% polyacrylamide gel (Mini Protean TGX, Biorad Laboratories). Proteins were transferred to a PVDF-FL membrane on a Biorad semidry blotting system ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble material was separated at 13,000 rpm for 30 minutes at 4 °C and total protein concentration was measured on supernatants using Bradford reagent (Bio-Rad). Protein extracts were denatured with NuPAGE®LDS (Invitrogen ...