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Citations for Bio-Rad :
6801 - 6850 of 7483 citations for Mouse Claudin 1 CLDN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated with ECL Western Blotting Substrate (Thermo, 32106) for 1 minute prior to imaging with the ChemiDoc system (BioRad).
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Neuroscience 2021Quote: ... A 1:5 dilution from serum samples was combined with 4 °C Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µg of cleaned RNA was used to generate cDNA with iScript reverse transcriptase per the manufacturer’s protocol (Bio-Rad). 20 µL quantitative real-time PCR reactions with 100 ng of cDNA input were carried out using TaqMan Fast Advance Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-IBA-1 antibody (0.4 μg/mL, FUJIFILM Wako) and rat anti-CD68 antibody (5 μg/mL, clone FA-11, Bio-Rad) was used ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were rinsed several times in 1xTBSTw and incubated with goat anti-rabbit HRP-conjugated secondary antibody (1:20,000; Bio-Rad, catalog 179-5046 ...
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bioRxiv - Molecular Biology 2019Quote: ... and goat anti-rabbit IgG H&L HRP conjugate at a dilution of 1:2000 (Bio-Rad, Hercules, CA, USA) was used as the secondary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... the blots were incubated with a secondary antibody conjugated to horseradish peroxidase (HRP) (1:5,000, Jackson Immuno Research Laboratories) and detected with a ChemiDoc XRS system (Bio-Rad). Primary antibodies used in western blot are listed below ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... immunoprecipitated chromatin and a 1:100 dilution of the input chromatin were separately recovered by boiling with a 10% Chelex-100 resin (BioRad) before treating with 25 μg/ml Proteinase K at 55 °C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with NaIO4 for 1 h then 15 min with NaS2O3 and mixed with 100 μl AffiGel Hz Hydrazide Gel (Biorad) overnight ...
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bioRxiv - Microbiology 2020Quote: ... The gel was run for 1 h at 100 V and visualized on a ChemiDoc MP Imaging System (BioRad, Germany).
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bioRxiv - Neuroscience 2019Quote: ... Each qPCR reaction was performed in triplicate using specific primers (Supplementary Table 1) and iQ SYBR Green Supermix (Bio-Rad) in a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Primary antibodies used for immunolabelling (Table 1) were utilized in combination with appropriate secondary antibodies purchased from BioRad (München, Germany) and BCIP-NBT (VWR international ...
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bioRxiv - Synthetic Biology 2019Quote: ... Pulsed-field electrophoresis was carried out using a 1% agarose gel in 0.5x TBE buffer on the CHEF-DR III system (Bio-Rad). The entire 10 µL of each above described reaction was mixed with 10 µL of molten (55°C ...
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bioRxiv - Microbiology 2019Quote: ... The nitrocellulose membrane was washed three times with 1x TTBS and then probed at a 1:20,000 dilution of HRP-conjugated goat α-rabbit antibody (Biorad) in 5% non-fat milk solution for one hour ...
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bioRxiv - Cancer Biology 2020Quote: ... ran at 120 V for ∼2 h and transferred at 100 V for 1 h onto nitrocellulose membranes (Bio-rad) using the wet blot method ...
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bioRxiv - Cell Biology 2019Quote: ... The labeled HA disaccharides were electrophoresed in a Bio-Rad mini-PROTEAN Tetra system using a gel composition of 20% acrylamide (37.5:1; Bio-Rad), 40 mM Tris acetate (pH 7.0) ...
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bioRxiv - Immunology 2021Quote: ... serially diluted rabbit serum (5-fold dilution starting with 1:80) were tested for binding to color-coded beads by Bio-plex (Biorad). The beads were coated with avi tagged C.1086 WT ...
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bioRxiv - Plant Biology 2020Quote: ... was used at a dilution of 1:2,000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:2,000) followed by a chemiluminescence reaction (Clarity Western ECL Substrate; Bio-rad). For the SS-TOM lines analysis ...
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bioRxiv - Microbiology 2021Quote: ... Gel was stained for 1 h with 0.1 μg/ml ethidium bromide and visualized by ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Detergent was removed by three addition of BioBeads SM-2 adsorbent resin (25 mg per 1 mL of lipid mixture) (BioRad) for 2 h each at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and eIF2B4 loci were amplified with the primer pairs detailed in Table 4 and run on a 1% agarose gel and imaged using a ChemiDoc XRS+ imaging system (Biorad). The expected WT fragment length for the eIF2B1 ...
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bioRxiv - Cell Biology 2021Quote: ... 15% for FKB-2) and transferred onto nitrocellulose membranes for 1 hour at 200 mA (SemiDry transfer blot, Bio-Rad). After incubation with blocking solution (LICOR Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was diluted 1:100 and real-time PCR was performed in triplicate with iQ SYBR Green Supermix from BioRad.
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was let dry and thereafter blocked on a shaker for 10 min in 15 ml blocking buffer (1% casein in TBS, BioRad). The blocking solution was discarded ...
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bioRxiv - Biophysics 2020Quote: ... samples were washed for two minutes with degassed polyacrylamide (PA) solution consisting of 4% (vol/vol) 19:1 acrylamide/bis-acrylamide (161010144, BioRad), 60 mM Tris-HCl pH 8 (AM9856 ...
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit IgG linked to peroxidase (dilution 1:5 000, Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane was treated with ECL (Immobilon Western chemiluminescence HRP substrate) for 1 min and visualized with the ChemiDoc MP imaging system (BioRad). Exposure was optimized for each blot.
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bioRxiv - Microbiology 2020Quote: ... Visualisation and quantification were carried out using a Personal Molecular Imager with Quantity One 1-D analysis software (Bio-Rad) and data were analyzed with GraphPad Prism 4 software.
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bioRxiv - Systems Biology 2020Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... 15 μL of each reaction was immediately loaded onto an 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad) supplemented with 2 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 μl of ChIP-ed or input (diluted 30-fold to bring it to 1%) DNA was used in 20 μl total volume reaction using SYBR green master mix (BioRad) and 0.4 μM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5,000, Bio-Rad 170-6515) or goat anti-mouse immunoglobulins (1:5,000 ...
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Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... Gel plugs were loaded onto 1% agarose gels and electrophoresed in the CHEF Mapper XA system (Bio-Rad, Hercules, CA) at 14℃ ...
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bioRxiv - Cell Biology 2021Quote: ... The Taqman probes (Suppl Table 1) used were the following: droplets were generated with a QX100 droplet generator (Bio-Rad), after mixing 20 µl of reaction mix and 70 µl of droplet generator oil (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
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bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
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bioRxiv - Microbiology 2022Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 h at 4 °C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on native acrylamide gels containing 0.5X TBE buffer and 7% acrylamide (made from a stock of 40% acrylamide with a ratio of 29:1 acrylamide:bisacrylamide; Bio-Rad). The running buffer for the gels was 1XTBE ...
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bioRxiv - Cancer Biology 2022Quote: ... and the pellet was resuspended in 1 ml of BM1* and cells were counted using a TC20 Automated Cell Counter (BioRad). Single-cell suspensions were mostly re-seeded at the same density as their parental/previous generation/passage.
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bioRxiv - Microbiology 2022Quote: ... centrifuged for 20 minutes at 4°C and supernatants mixed 3:1 with 4x Laemmli sample buffer (Bio-rad 1610747). Samples were heated at 95°C for 5 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The protein sample was incubated at 40°C and 80 °C respectively for 1 h in a PCR cycler (BioRad) with a heated lid to prevent evaporation ...
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bioRxiv - Bioengineering 2022Quote: ... Tricine-SDS-PAGE experiments were performed using a single layer 0.75 mm thick 20% acrylamide/bis-acrylamide gel (using 29:1 Acrylamide/Bisacrylamide, Bio-Rad) run at 120 V constant voltage (Pharmacia LKB ...
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bioRxiv - Biochemistry 2021Quote: ... The same samples were also detected by HRP conjugated StrepTactin antibody at 1:10000 dilution (Bio-Rad catalog number 161038). To allow detection of endogenous selenok ...
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bioRxiv - Molecular Biology 2019Quote: ... The agarose plugs were then loaded onto a 1% agarose gel and electrophoresed in 0.5% Tris-borate-EDTA in a pulse-field apparatus (Bio-Rad) at 14 °C (6 V/cm ...
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bioRxiv - Genomics 2020Quote: ... Membranes were washed using tris-based saline buffer solutions with 0.1% tween-20 (TBST) and blocking solutions were prepared using 5% w/v western-blot grade dry milk powder (Biorad). Membranes were blocked with blocking buffer for 1 hour and washed between incubations for 5-10 mins (3x) ...
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A gut-secreted peptide controls arousability through modulation of dopaminergic neurons in the brainbioRxiv - Neuroscience 2020Quote: ... DNA product was diluted 1:10 and used to set up quantitative PCR reactions using Sybr Green (Biorad #170-8880). Primers were obtained from ID Technologies:
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bioRxiv - Plant Biology 2021Quote: ... in TBS-T (TBS supplemented with 0.1% (v/v) Tween-20) and 1% (w/v) Blotting-Grade Blocker (Bio-Rad) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μL of electrocompetent cells were transformed with 1 ng of the lesion-containing plasmid mixed with 1 ng of pVP146 (internal control for transformation efficiency) by electroporation using a GenePulser Xcell (BioRad) at 2.5 kV ...