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601 - 650 of 2247 citations for Phosphatidylinositol N acetylglucosaminyltransferase subunit Q PIGQ Antibody HRP since 2020

• A pathway to produce non-coding piRNAs from endogenous protein-coding regions supports Drosophila spermatogenesis

  Taichiro Iki, Shinichi Kawaguchi, Toshie Kai, bioRxiv - Molecular Biology 2022

  Quote: ... anti-mouse IgG-HRP (BioRad, 1:3000). The chemiluminescent signals were obtained by using Chemi-Lumi One (Nacalai) ...
• Overexpression of the WWE domain of RNF146 modulates poly-(ADP)-ribose dynamics at sites of DNA damage

  Rasha Q. Al-Rahahleh, et al., bioRxiv - Molecular Biology 2023

  Quote: ... goat anti-rabbit-HRP conjugate (Bio-Rad), and HRP- conjugated streptavidin (Thermo Fisher Scientific ...
• Functional characterization of CCHamides and deorphanization of their receptors in the yellow fever mosquito, Aedes aegypti

  Jinghan Tan, Susanne Neupert, Jean-Paul Paluzzi, bioRxiv - Zoology 2024

  Quote: ... anti-rabbit-HRP (Bio-Rad Laboratories (Canada) Ltd ...
• FITM2 deficiency results in ER lipid accumulation, ER stress, reduced apolipoprotein B lipidation, and VLDL triglyceride secretion in vitro and in mouse liver

  Haizhen Wang, et al., bioRxiv - Biochemistry 2023

  Quote: ... Goat anti-rabbit conjugated to HRP (BioRad) and donkey anti-goat conjugated to 800CW (Li-Cor ...
• Distinct hyperactive RAS/MAPK alleles converge on common GABAergic interneuron core programs

  Sara J Knowles, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-rabbit HRP (BioRad 170-6515) and goat anti-mouse HRP (BioRad 170-6516).
• A remodeled RNA polymerase II complex catalyzing viroid RNA-templated transcription

  Shachinthaka D. Dissanayaka Mudiyanselage, et al., bioRxiv - Plant Biology 2022

  Quote: ... HRP-conjugated anti-mouse serum (Bio-Rad) was diluted at 1:5,000 ...
• Cryptochromes and UBP12/13 deubiquitinases antagonistically regulate DNA damage response in Arabidopsis

  Yuzhao Hu, et al., bioRxiv - Plant Biology 2023

  Quote: ... and goat-anti-rabbit-HRP (Bio-Rad) were used as secondary antibodies ...
• Musashi-2 causes cardiac hypertrophy and heart failure by inducing mitochondrial dysfunction through destabilizing Cluh and Smyd1 mRNA

  Sandhya Singh, et al., bioRxiv - Cell Biology 2023

  Quote: ... and Precision protein Streptactin-HRP conjugate (BioRad) were used to determine protein size on the membrane.
• Chemoresistance to additive PARP/PI3K dual inhibition in triple-negative breast cancer cell lines is associated with adaptive stem cell-like prevalence

  Paula E. Petrella, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and HRP-Streptactin Conjugate (Bio-Rad, #1610380) at 1:10,000 dilution in TBST for 1 hr at RT ...
• Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin

  Zachary T. Berndsen, et al., bioRxiv - Microbiology 2024

  Quote: ... and incubated with avidin-HRP conjugate (BioRad 1706528 ...
• Serpina1e mediates the exercise-induced enhancement of hippocampal memory

  Hyunyoung Kim, et al., bioRxiv - Neuroscience 2024

  Quote: ... HRP-conjugated anti-rabbit IgG (Bio-Rad), streptavidin-HRP (Thermo Scientific ...
• Loss of TDP-43 induces synaptic dysfunction that is rescued by UNC13A splice-switching ASOs

  Matthew J. Keuss, et al., bioRxiv - Neuroscience 2024

  Quote: ... Goat anti-Mouse HRP (Bio-Rad 1706516) 1:10,000 Goat anti-Rabbit HRP (Bio-Rad 1706515 ...
• Catalytic-dependent and independent functions of the histone acetyltransferase CBP promote pioneer factor-mediated zygotic genome activation

  Audrey J. Marsh, et al., bioRxiv - Developmental Biology 2024

  Quote: ... and goat anti-mouse IgG-HRP (BioRad) were used at 1:3000 and incubated at 1 hour at room temperature ...
• Cellular and structural basis of synthesis of the unique intermediate dehydro-F₄₂₀-0 in mycobacteria

  Rhys Grinter, et al., bioRxiv - Biochemistry 2020

  Quote: ... The clarified supernatant was applied to a High Q Anion Exchange Column (Biorad) equilibrated in 50 mM Tris (pH 7.5) ...
• A secreted NlpC/P60 endopeptidase from Photobacterium damselae subsp. piscicida cleaves the peptidoglycan of potentially competing bacteria

  Johnny Lisboa, et al., bioRxiv - Microbiology 2020

  Quote: ... followed by anion-exchange chromatography (Bio-Scale Mini Macro-Prep High Q; BioRad). Fractions containing the recombinant proteins were pooled and injected into a size-exclusion chromatography column (Superose12 10/300 GL ...
• Elicitation of potent SARS-CoV-2 neutralizing antibody responses through immunization using a versatile adenovirus-inspired multimerization platform

  C Chevillard, et al., bioRxiv - Microbiology 2021

  Quote: ... NaCl 150 mM and then loaded onto a Macroprep Q cartridge (Bio-rad). After elution by a 150 to 600 mM linear NaCl gradient in Hepes 10mM pH 7.4 ...
• ATP6AP2-to-MMP14, a key pathway for osteoblast to osteocyte transition

  Lei Xiong, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Q-PCR was performed by using Quantitect SYBR Green PCR Kit (Bio-Rad) with a Real-Time PCR System (Opticon Monitor 3) ...
• Differences in Gut Microbiota Assembly Alter Its Ability to Metabolize Dietary Polysaccharides and Resist Clostridioides difficile Colonization

  Matthew K. Schnizlein, et al., bioRxiv - Microbiology 2022

  Quote: ... Milli-Q water through an Aminex HPX87H column (Bio-Rad Laboratories, Hercules, CA). Sample areas under the curve were compared to volatile fatty acid standards with concentrations of 40 ...
• Molecular mechanism of Arp2/3 complex inhibition by Arpin

  Fred E. Fregoso, et al., bioRxiv - Biochemistry 2021

  Quote: ... The supernatant was loaded onto a Macro-Prep High Q column (Bio-Rad) pre-equilibrated with Arp buffer ...
• Impacts of Disinfection Methods in a Granular Activated Carbon (GAC) Treatment System on Disinfected Drinking Water Toxicity and Antibiotic Resistance Induction Potential

  Yinmei Feng, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... Q-PCR was performed in triplicate using SYBR Green Supermix (Bio-Rad, U.S.A.) on iQ7 Multicolor Real-Time PCR detection system (Bio-Rad ...
• Insulin-Independent Regulation of Type 1 Diabetes via Brown Adipocyte-Secreted Proteins and the Novel Glucagon Regulator Nidogen-2

  Jeongmin Lee, et al., bioRxiv - Cell Biology 2024

  Quote: ... Goat Anti-Rabbit IgG (H L)-HRP Conjugate or Goat Anti-Mouse IgG (H L)-HRP Conjugate (Bio-Rad) was incubated with the blot for 1□h at room temperature (RT) ...
• Cardiolipin targets a dynamin related protein to the nuclear membrane

  Usha Pallabi Kar, Himani Dey, Abdur Rahaman, bioRxiv - Cell Biology 2020

  Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
• Neuronal post-developmentally acting SAX-7S/L1CAM can function as cleaved fragments to maintain neuronal architecture in C. elegans

  V.E. Desse, et al., bioRxiv - Developmental Biology 2021

  Quote: ... an affinity purified antibody generated against the SAX-7 cytoplasmic tail [gift of (Chen et al., 2001)] and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). For the loading control ...
• Comparative therapeutic strategies for preventing aortic rupture in a mouse model of vascular Ehlers Danlos syndrome

  Anne Legrand, et al., bioRxiv - Genetics 2021

  Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
• TRPC3 and NALCN channels drive pacemaking in substantia nigra dopaminergic neurons

  Ki Bum Um, et al., bioRxiv - Neuroscience 2021

  Quote: ... the membranes were incubated for 2 hours at room temperature with HRP-conjugated rabbit secondary antibody (Bio-Rad, USA, 170-6515; RRID: AB_11125142) for NALCN detection and HRP-conjugated mouse secondary antibody (Bio-Rad ...
• The bacterial virulence factors rhamnolipids and their (R)-3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms

  Romain Schellenberger, et al., bioRxiv - Plant Biology 2021

  Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
• In vitro tau aggregation inducer molecules influence the effects of MAPT mutations on aggregation dynamics

  David J. Ingham, et al., bioRxiv - Biochemistry 2021

  Quote: ... A goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) antibody with HRP conjugate (1706515 and 1706516, respectively, Bio-Rad, Hercules, CA) was used as a secondary detection antibody ...
• Nigral-specific increase in ser31 tyrosine hydroxylase phosphorylation offsets dopamine loss and forestalls hypokinesia onset during progressive nigrostriatal neuron loss

  Ella A. Kasanga, et al., bioRxiv - Neuroscience 2022

  Quote: ... Secondary antibodies used were Immun-Star goat anti-rabbit (GAR)-HRP Conjugate (Cat # 170-5046, or Cat#170-6515; Bio-Rad Laboratories, USA). Clarity Western ECL Substrate (cat #170-5061 ...
• Long-chain fatty acyl-coenzyme A activates the mitochondrial fission factors MiD49 and MiD51 by inducing their oligomerization

  Ao Liu, et al., bioRxiv - Cell Biology 2023

  Quote: ... the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti mouse #1721011; Bio-Rad or goat anti rabbit #1706515; BioRad) for 1 h at room temperature ...
• The effects of phenylalanine and tyrosine levels on dopamine production in rat PC12 cells. Implications for treatment of phenylketonuria and tyrosinemia type 1

  Peter D. Szigetvari, et al., bioRxiv - Molecular Biology 2023

  Quote: ... primary antibodies and proteins were detected by incubation at a 1:5000 dilution with secondary horseradish peroxidase (HRP)- conjugated antibodies (#170-6516, goat anti-mouse and 170-6515; goat anti-rabbit from Bio-Rad, Hercules, USA). The reactive protein bands were visualized using the WesternBright ECL HRP substrate (Advansta ...
• Metabolic rewiring of mitochondria in senescence revealed by time-resolved analysis of the mitochondrial proteome

  Jun Yong Kim, et al., bioRxiv - Cell Biology 2022

  Quote: ... Then membranes were washed 3 times with TBS-T and incubated with goat anti-mouse or anti-rabbit IgG HRP-conjugated secondary antibody (1:10000 in 5% BSA TBS-T, Bio-rad; #1706515, #1706516) for 1 h ...
• MYB and ELF3 differentially modulate labor-inducing gene expression in myometrial cells

  Virlana M. Shchuka, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Membranes were washed three times in TBST prior to incubation with secondary antibody (1/5000 dilution horseradish peroxidase (HRP)-conjugated anti-mouse (Bio-Rad, 170-6516) or anti-rabbit (Bio-Rad ...
• A multidimensional atlas of human glioblastoma organoids reveals highly coordinated molecular networks and effective drugs

  Changwen Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... the membranes were incubated with respective horseradish peroxidase (HRP) conjugated secondary antibodies for two hours and imaged using ChemiDoc (Bio-Rad, California, USA) after reacting with HRP substrate ...
• Redistribution of fragmented mitochondria ensure symmetric organelle partitioning and faithful chromosome segregation in mitotic mouse zygotes

  Haruna Gekko, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) was used as a secondary antibody (1:2000) for chemiluminescence detection (Clarity Western ECL Substrates, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
• Heme allocation in eukaryotic cells relies on mitochondrial heme export through FLVCR1b to cytosolic GAPDH

  Dhanya Thamaraparambil Jayaram, et al., bioRxiv - Cell Biology 2024

  Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
• The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior

  Donatella Lobraico, et al., bioRxiv - Neuroscience 2024

  Quote: ... Then washed and incubated with the following peroxidase-conjugated secondary antibodies at room temperature for 45 min: goat anti-rabbit IgG HRP (1:3000; Bio-Rad, California, USA) and goat anti-mouse IgG HRP (1:3000 ...
• Oxidative Injury to Lung Mitochondrial DNA is a Key Contributor for the Development of Chemical Lung Injury

  Shubham Dubey, et al., bioRxiv - Physiology 2024

  Quote: ... the membrane was incubated with HRP-conjugated secondary antibodies against mouse (Cat No. 1721011, Bio- Rad Hercules, CA) and rabbit (Cat No. 1706515, Bio-Rad, Hercules, CA) at room temperature for 1 hour ...
• Mycobacterial resistance to zinc poisoning requires assembly of P-ATPase-containing membrane metal efflux platforms

  Yves-Marie Boudehen, et al., bioRxiv - Microbiology 2021

  Quote: ... Signals were detected by autoradiography using HRP reaction (WesternBright Quantum HRP Substrate for CCD, Diagomics) on a ChemiDoc Touch Imaging System (BioRad).
• A non-canonical role for the autophagy machinery in anti-retroviral signaling mediated by TRIM5α

  Bhaskar Saha, Devon Chisholm, Michael A Mandell, bioRxiv - Cell Biology 2020

  Quote: ... HRP-conjugated goat anti-mouse (Bio-Rad, 1721011) and goat anti-rabbit (Bio-Rad ...
• Structural and functional characterization of the Spo11 core complex

  Corentin Claeys Bouuaert, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HRP-conjugated goat anti-rabbit IgG (Bio-Rad), HRP-conjugated donkey anti-goat IgG (Santa Cruz) ...
• Structural and functional characterization of the Spo11 core complex

  Corentin Claeys Bouuaert, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HRP-conjugated goat anti-mouse IgG (Bio-Rad), HRP-conjugated goat anti-rabbit IgG (Bio-Rad) ...
• Sensitivity to Monoclonal Antibody 447-52D and an Open Env Trimer Conformation Correlate Poorly with Inhibition of HIV-1 Infectivity by SERINC5

  Aaron O. Angerstein, et al., bioRxiv - Molecular Biology 2020

  Quote: ... or rabbit anti-sheep-HRP (Bio-Rad Laboratories) secondary antibodies (diluted 1:3000) ...
• Inflammation Drives Alternative First Exon usage to Regulate Immune Genes including a Novel Iron Regulated Isoform of Aim2

  Elektra K. Robinson, et al., bioRxiv - Immunology 2020

  Quote: ... HRP-conjugated goat anti-rabbit (1:1,500, Biorad) secondary antibodies were used ...
• Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly

  Christian Hentrich, et al., bioRxiv - Biochemistry 2020

  Quote: ... anti-His5-Tag IgG-HRP (MCA5995P, Bio-Rad), anti-FLAG-Tag (M2 ...
• Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly

  Christian Hentrich, et al., bioRxiv - Biochemistry 2020

  Quote: ... anti-His5-Tag IgG-HRP (MCA5995P, Bio-Rad), anti-FLAG-Tag (M2 ...
• Interactomic analysis reveals a new homeostatic role for the HIV restriction factor TRIM5α in mitophagy

  Bhaskar Saha, et al., bioRxiv - Cell Biology 2021

  Quote: ... HRP-conjugated goat anti-mouse (Bio-Rad, 1721011) and goat anti-rabbit (Bio-Rad ...
• WNT inhibition creates a BRCA-like state in Wnt-addicted cancer

  Amanpreet Kaur, et al., bioRxiv - Cancer Biology 2020

  Quote: ... HRP conjugated anti-rabbit IgG (Bio-Rad 1706515) or anti-mouse IgG (Bio-Rad 1721011 ...
• RHBDL4 protects against endoplasmic reticulum stress by regulating the morphology and distribution of ER sheets

  Viorica L. Lastun, et al., bioRxiv - Cell Biology 2021

  Quote: ... goat polyclonal anti-rabbit-HRP (Biorad, 170-6515), mouse monoclonal anti-goat-HRP (Santa Cruz ...
• CRISPR-Mediated Generation and Characterization of a Gaa Homozygous c.1935C>A (p.D645E) Pompe Disease Knock-in Mouse Model Recapitulates Human Infantile Onset-Pompe Disease

  Shih-hsin Kan, et al., bioRxiv - Genetics 2022

  Quote: ... before applying ECL HRP substrate (Bio-Rad 1705060) for chemiluminescence ...
• Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD finger

  Keiichi Hata, et al., bioRxiv - Biochemistry 2022

  Quote: ... goat anti-rabbit HRP (polyclonal; 1:5000; BioRad), rabbit anti-mouse HRP (1:5000 ...