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Citations for Bio-Rad :
601 - 650 of 1820 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed 5 X 5 min in TBST and signals were detected using the Clarity Western ECL Substrate (Biorad #1705060). At least 2 separate gels were immunoblotted with cortical extracts from independent litters and used for quantitation.
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bioRxiv - Microbiology 2023Quote: ... with 10 μg of plasmid DNA expressing LpAsp-GFP or LpCht-GFP as well as pTrex-n-eGFP using a Gene Pulser X cell electroporator (Bio-Rad) and cuvette (2 mm gap) ...
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bioRxiv - Cell Biology 2023Quote: ... such that a fluorescent construct can be used as an expression reporter for cells co-transfected with a non-fluorescent construct.1 Cells were electroporated at 280 V and 950 μF using Gene Pulser X (Bio-Rad), then immediately resuspended in 6 ml medium and cultured in MatTek dishes (2 ml/dish ...
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bioRxiv - Microbiology 2023Quote: ... Lactose and salts were removed by gel filtration chromatography over a BioRad P2 column (100 cm x 316 mm, Bio-Rad) using a semi-automated fast protein liquid chromatography (FPLC ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Immunology 2023Quote: ... Specific proteins were detected in the dark room on the medical X-ray film blue (Agfa) with an Agfa brand developer and fixer or using the ChemiDoc MP Imaging System (Bio-Rad). List of antibodies and all other materials and reagents is provided in Supplementary Table S2 in Supplementary Materials.
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bioRxiv - Biochemistry 2023Quote: ... The samples were then spun for 1 min at 20k x g and run on a 10% SDS gel before transferring to nitrocellulose membranes (Bio-Rad) and blocking as described above ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and treated with a lysis buffer (150mM NaCl, 50mM Tris pH7.5, 1% Triton X-100 (Bio-Rad #1610407), 1% SDS ...
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bioRxiv - Molecular Biology 2024Quote: ... and exposed to an X-ray film (catalog no. 28906839, Cytiva) or detected using a digital chemiluminescence imager (catalog no. 17001402JA, Bio-Rad).
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bioRxiv - Biophysics 2023Quote: ... UBQLN1 D15R and UBQLN1 E21Q were subjected to size exclusion chromatography over an ENrich™ SEC 650 10 x 300 column (BioRad) in 20 mM sodium phosphate buffer pH 6.8 ...
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bioRxiv - Biochemistry 2023Quote: Analytical size exclusion chromatography was carried out on a Waters HPLC with an ENrichTM SEC 650 10 x 300 column (Bio-Rad) in 20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... brucei PFs were prepared in 1% Triton X-100 in PBS with 1X protease inhibitor cocktail (Bio-Vision) and mixed in 4X Laemmli buffer (Bio-Rad) with 710 mM β-mercaptoethanol and heated for 5 min at 95°C ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from ∼10 x 106 cells of each experiment using PureZOL RNA Isolation reagent following the manufacturer (BIO-RAD) protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were cleared by centrifugation (10 min at 14,000 x g, 4°C) and quantified by Bradford (Bio-Rad, cat#5000205). All samples were then boiled for 5 min in Laemmli sample buffer ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR assays were conducted using gene specific primers (Supplementary table X. Primer list) using SYBR green master mix (Bio-Rad). The reaction was run for a total of 40 cycles ...
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bioRxiv - Cancer Biology 2021Quote: ... 8% of 2% w/v bis-acrylamide (Bio-Rad, #1610142), 0.5% of 10% ammonium persulfate (APS ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were boiled with 2× Laemmli sample buffer (Bio-Rad) containing 2.5% β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2019Quote: 2% certified low melt agarose (Bio-Rad Laboratories, Cat. # 1613111) was prepared with L Buffer and kept at 75°C ...
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bioRxiv - Biophysics 2019Quote: ... Bio-Beads (Bio-Beads SM-2 Absorbent Media, Bio-Rad) were added to the reconstitution mixture for 1 hour then fresh Bio-Beads were added and the mixture was allowed to incubate overnight ...
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bioRxiv - Microbiology 2019Quote: ... and 200 ohms resistance in a 2 mm cuvette (BioRad). Cells were plated in MEM supplemented with non-essential amino acids ...
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bioRxiv - Cancer Biology 2019Quote: ... 6’,-diamidino-2-phenylindole (DAPI; BIO-RAD, Hercules, CA, USA) for 30’ at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... diluted 2:1 in 4X Laemmli sample buffer (Bio-Rad) containing 100 mM Dithiothreitol (CAS No ...
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bioRxiv - Plant Biology 2021Quote: ... for 2 min and detected by ChemiDoc imaging system (Biorad). For equal loading analysis ...
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bioRxiv - Biophysics 2020Quote: ... and 2% bis-acrylamide (#161-0142, Bio-Rad, Munich, Germany) in PBS that are stored at 4°C for a maximum time of 2 months ...
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bioRxiv - Cancer Biology 2021Quote: ... using 2× iQ Custom Sybr Green Supermix (Bio-Rad Laboratories). Values were normalized on mRNA expression of human β-actin and HPRT (reference genes) ...
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bioRxiv - Biochemistry 2021Quote: ... 200 mg Bio-Beads™ SM-2 Resin (Bio-Rad) was added and incubated at 4°C overnight while shaking at 650 rpm ...
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bioRxiv - Genomics 2022Quote: ... The lysate was added to 2 chromatography columns (7321010, BioRad) packed with 25 mL each of chitin resin (S6651S ...
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bioRxiv - Microbiology 2022Quote: ... Capacitors were 1-cm Tygon tubing (2 mm ID, BioRad), having Luer connectors on each extremity ...
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bioRxiv - Microbiology 2022Quote: ... Extracts were diluted with 2 × Laemmli sample buffer (Bio-Rad) and β-mercaptoethanol (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... Extracts were diluted with 2□×□Laemmli sample buffer (Bio-Rad) and β-mercaptoethanol (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... goat anti-human IgG F(ab’)2 (STAR126, Bio-Rad), anti-M13-pVIII-HRP (Cytiva 27-9421-01 ...
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bioRxiv - Microbiology 2023Quote: ... Detergent was removed using adsorbing SM-2 Biobeads (Bio-Rad): the protein-lipid mix was incubated twice with fresh Biobeads at 10:1 (w:w ...
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bioRxiv - Microbiology 2023Quote: ... and β-tubulin (clone YL1/2, Bio-Rad; 1:5000) antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were prepared by utilizing 2 × Laemmli buffer (Bio-Rad). Proteins from mouse brain tissue were separated on 8%–16% and 4%–20% gradient SDS-PAGE gels before being transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2023Quote: ... including 7.5 μl of 2× iQSYBR Green Mix (Bio-Rad) and 100–300 nM forward and reverse primers ...
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bioRxiv - Neuroscience 2023Quote: ... 60 mg of Bio-Beads SM-2 (#1523920, Bio-Rad) was added ...
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bioRxiv - Systems Biology 2024Quote: ... buffer + 2% (v/v) Sodium dodecyl sulfate (SDS, Bio-Rad). Proteins were denatured at 95 °C and 600 rpm for 10 min before adding a final concentration of 2% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... then detergent was removed with BioBeads SM-2 (Bio-Rad) at 80 mg/ml ...
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bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).
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bioRxiv - Immunology 2019Quote: ... fatty acid free BSA Efflux was measured after a 4-hour incubation period with 0µg/ml and 50µg/ml of human HDL (#5685-2004, BioRad). Supernatants were collected and the cells lysed with 1% (w/v ...
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bioRxiv - Plant Biology 2019Quote: ... A Bio-Rad Aminex HPX-87H (300 x 7.8 mm, 9 μm particle size) column with a Micro-Guard Cation H guard column (Bio-Rad, Watford, UK) was used to achieve separation with an isocratic gradient of 5 mM sulfuric acid ...
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bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
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bioRxiv - Cell Biology 2022Quote: ... for 20 minutes treated with PBS containing 1 % w/v BSA and 0.1 % v/v Triton X-100 (Bio-Rad, München, Germany) for 30 minutes ...
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bioRxiv - Genetics 2022Quote: ... Signals were visualized using X-ray film and a film processor (pSer63 c-Jun) or using Chemidoc™ MP Imaging System (Bio-Rad). Membranes were re-incubated with anti α-Tubulin (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: Effluents from control and acidic pHEX reperfused hearts were analyzed by HPLC (Shimadzu Prominence 20A system) using two 300 x 7.8 mm Aminex HPX-87H columns (BioRad, Carlsbad CA, USA) in series ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged 10 minutes at 13,000 x g and the supernatant was quantified using a DC assay kit (Bio-Rad, Hercules, California). 200 µg of total protein were diluted into a total of 1.5 mL Array Buffer and rocked over the pre-blocked array membrane containing various total RTK antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed again with TBST buffer for 5 times X 5 min and developed using Clarity Western ECL substrate (Bio-Rad, 1004384863). Images were captured on a Luminescent Image Analyzer (GE Healthcare ...