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Citations for Bio-Rad :
601 - 650 of 2583 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... using a S3e TM Cell Sorter (BIORAD) endowed with a 488 nm laser and a 525/30 nm emission filter ...
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bioRxiv - Cell Biology 2024Quote: ... and TC20 Automated Cell Counter (Bio-Rad). Supernatant was centrifuged at 3,000 x g for 30 min (unless otherwise noted ...
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bioRxiv - Biochemistry 2024Quote: ... or the Mini Trans-Blot Cell (Biorad), blocked in TBS-T with 2,5% BSA for 30 minutes at room temperature and incubated in the primary antibody over night at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... cells from the bottom of the filter were trypsinized and counted using an automated cell counter (TC20; Bio-Rad). Transmigration was then calculated as the ratio of cells on the bottom of the filter over the total.
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bioRxiv - Cancer Biology 2019Quote: ... Trypsin was inactivated and Trypan Blue Exclusion cell counting was performed using a TC10 Automated Cell Counter (Bio-Rad). For long-term spheroid growth assays ...
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bioRxiv - Biochemistry 2020Quote: ... Cell number and viability was determined by trypan blue staining and using a TC20 Automated Cell Counter (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: Cells were filtered through a 40 µm mesh and loaded in a ZE5 Cell Analyzer flow cytometer (Bio-Rad). Data was collected for forward scatter and side scatter using the 488 nm laser ...
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bioRxiv - Bioengineering 2022Quote: ... emission 662 nm) signals were recorded for 10k gated single-cell events by an S3e Cell Sorter (BioRad, USA). Mean FL-4 fluorescence signal values of RBD+ cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell number and viability was determined by trypan blue staining and using a TC20 Automated Cell Counter (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... we used 10µL of desalted assembly reaction products to transform competent E.coli S17-1 cells (mid-log cells, washed three times in deionized ice-cold water) by electroporation (V=1.8kV; MicroPulser, BioRad). After 1h recovery at 37°C in 1mL of LB ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were stained by Trypan Blue and automatically counted using a TC20 Automated Cell Counter (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2020Quote: ... EV from 20×106 cells (or specified number of particles) and CL from 0.2×106 cells were mixed with Laemmli sample buffer (BioRad), including 10% of β-mercaptoethanol (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Total numbers of viable cells were determined after 2 and 4 days using the TC10 cell counter (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell culture viability and density were determined with an Automated Cell Counter TC20™ (Bio-Rad Laboratories, Sundyberg, Sweden) by the addition of 0.2% trypan blue (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: At day 16 of differentiation cells were lifted using Accutase and counted using TC20™ Automated Cell counter (BioRad). Cells were centrifuged (300g ...
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bioRxiv - Microbiology 2021Quote: ... Cell concentration was determined for three independent cultures of each strain using the TC20 automated cell counter (Bio-Rad). These results were used to normalize the total areas of each chemical feature detected ...
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bioRxiv - Neuroscience 2022Quote: ... the number of cells was counted using a Bio-Rad TC20 automated cell counter (Bio-Rad Laboratories, California, USA), using trypan blue (Cat#302643 ...
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bioRxiv - Immunology 2022Quote: The viability of cells was assessed by trypan blue and counted in an automated TC20 cell counter (Bio-Rad) prior libraries preparation ...
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bioRxiv - Systems Biology 2022Quote: ... The cells were sorted using the BD FACS Aria as single cells into a 96-well plate (Bio-Rad) containing 4μl lysis buffer (0.4% Triton X-100 in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Cell number and viability were assessed by Trypan Blue (0.2%) staining using a TC10 automated cell counter (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2022Quote: ... The viability and concentration of cells after sort were measured using the TC20 Automated Cell Counter (#1450102, Bio-Rad). The viability of the cells pre-loading was > 90% ...
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bioRxiv - Immunology 2023Quote: Equivalent number of T cells (1E6 cells/group) were lysed in Laemmli Sample Buffer with ²-mercaptoethanol (Bio-Rad, CA). Total cell lysate for each sample were resolved on Bis-Tris 4–12% gel (ThermoFisher NuPAGE ...
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bioRxiv - Bioengineering 2023Quote: ... Old + siPiezo1) were harvested by enzymatic dissociation and cell viability was confirmed using an automated cell counter (Biorad, TC20). Cell hashing with anti-mouse oligo-tagged antibodies (TotalSeq A ...
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bioRxiv - Cell Biology 2023Quote: ... For single cell RNA-sequencing cells were sorted with an 85um nozzle into 96-well PCR plates (Bio-Rad) containing 2ul of lysis buffer [0.2% Triton X-100 ...
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bioRxiv - Biochemistry 2023Quote: ... The median fluorescence intensity of living single cells was determined in triplicates using a ZE5 Cell Analyzer (Bio-Rad) and FCS Express (De Novo Software ...
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bioRxiv - Cancer Biology 2024Quote: Cells were first embedded in gel plugs with approximately 1 million cells per gel plug (BioRad no. 170-3592). The high molecular weight genomic DNA was extracted and purified using the Bionano Genomics SP kit.
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bioRxiv - Biophysics 2021Quote: ... and total cell counts in BALF were determined using the TC20 automated cell counter (Bio-Rad Laboratories, Inc., Hercules, CA). The differential cell counts were performed on cells cytocentrifuged onto glass slides (Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... GFP positive cells were single cell sorted using 85 micron nozzle in a FACSAriaIII machine into 96 well plates (Biorad) containing 30µl lysis buffer (2.5µl 0.1M Dithiothreitol ...
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bioRxiv - Immunology 2022Quote: Cell viability was determined by 0.4% Trypan Blue dye exclusion test executed by a TC20 Automated Cell Counter (Bio-Rad).
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bioRxiv - Genomics 2020Quote: ... was added to the cell pellet and the cell mixture was transferred to a 96-well PCR plate (Bio-Rad). The lysate was heated for 15 minutes at 65°C followed by 10 minutes at 98°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Cell number was determined using trypan blue exclusion and quantification in a cell counter (TC-20, Bio-Rad, Vienna, Austria).
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bioRxiv - Cancer Biology 2019Quote: 150,000 cells were seeded per T25 flask in mNSC media and counted after 72 hours on a TC20 cell counter (Biorad) using Trypan blue stain (Thermo) ...
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bioRxiv - Bioengineering 2021Quote: ... The cell pellet was re-suspended in 10mL of fresh culture media (37°C) and cells density was calculated with a TC20 automated cell counter (BioRad). 100’000 to 150’000 cells were plated per 35mm glass-bottom dish and transfected the following day ...
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bioRxiv - Cancer Biology 2021Quote: The cells were counted and examined for viability with trypan blue staining using a TC-20 cell counter (Bio-Rad). Approximately 10,000 live cells with above 70% viability were loaded into the Single Cell Chip to generate single cell emulsion in Chromium Controller (10x Genomics ...
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bioRxiv - Biochemistry 2022Quote: ... The percentage of live cells was determined using trypan blue staining quantified using a TC10 Automated Cell Counter (Bio-Rad). To determine intracellular NADPH and oxidized glutathione (GSSG) ...
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bioRxiv - Cell Biology 2022Quote: siRNAs-treated HeLa cells and Control KO and Cavin1 KO cells were directly lysed in 1x Laemmli (Bio-Rad Laboratories) with Benzonase nuclease (#70664 Millipore ...
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bioRxiv - Developmental Biology 2022Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833 ...
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bioRxiv - Cancer Biology 2022Quote: ... and the pellet was resuspended in 1 ml of BM1* and cells were counted using a TC20 Automated Cell Counter (BioRad). Single-cell suspensions were mostly re-seeded at the same density as their parental/previous generation/passage.
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bioRxiv - Immunology 2020Quote: ... Cells were diluted in mock cells (electroporated without gRNA and then transduced) if needed and counted in a TC-20 automatic cell counter (Biorad). For immunizations ...
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bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability (Trypan blue exclusion) was confirmed to be greater than 90% using the TC20 Automated Cell Counter (Bio-Rad). Cell pellets were washed twice in PBS containing 0.04% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were trypsinized with 0.25% trypsin-EDTA and cells in suspension were counted using an automated cell counter (TC20, Bio-Rad) and resuspended in medium to a density of 5×105 cells/ml ...
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bioRxiv - Genetics 2020Quote: ... LSK were plated at day 8 of culture and monitored for growth by counting live cells by Trypan blue exclusion using a TC20TM Automated Cell Couter (BIORAD) at the indicated time ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 500 μl buffer and counted with trypan blue in a TC20 automated cell counter (BioRad). Cells were stained in the presence of a Fcγ-receptor blocker ...
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bioRxiv - Cell Biology 2019Quote: For detecting phosphorylation of ERK1/2 in both HEK-293 cells and knockdown cells Western blotting analysis of the whole-cell lysate was performed and transferred to polyvinylidene difluoride membranes (PVDF;BioRad). The membrane was blocked with 5% BSA (SRL ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of live cells was determined every day by trypan blue exclusion using a TC20 Automated Cell Counter (BioRad). Cell density was maintained at 1.0×106 cells/mL.
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bioRxiv - Cell Biology 2021Quote: ... Cell counting and viability assays were performed with trypan blue staining on an automated cell counter (Bio-Rad TC-20). Cells were cultured for indicated times after drug treatment and harvested as indicated for various assays.
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bioRxiv - Cell Biology 2020Quote: ... The amount of ATP for one cell was derived from the amount of protein in one cell found via Bradford assay (BioRad).
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bioRxiv - Cancer Biology 2020Quote: ... Around 40 μg of whole-cell lysate was resolved in 10% SDS-PAGE gel (Mini protein Tetra cell Bio-Rad) and blotted to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... An aliquot of cells was stained with trypan blue and counted using Automated Cell Counter (Bio-Rad, Hercules, California, USA). Isolated PBMCs were then cryopreserved in a cryovial in cell recovery freezing medium containing 10% Dimethyl Sulfoxide (DMSO ...