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Citations for Bio-Rad :
601 - 650 of 4394 citations for 7 BROMO 1 METHYL 1H QUINOXALIN 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 95°C for 2 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min (BioRad). The following primers from Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Cancer Biology 2023Quote: ... qPCR duplicates were carried out using 2 µL of diluted cDNA (1 into 100) in a 20 µL-reaction using Sso Fast EvaGreen reagent (BioRad) following supplier’s instructions on a StepOne Plus™ Real-Time PCR system (Applied BioSystems) ...
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bioRxiv - Immunology 2023Quote: ... Bio-Plex SARS-CoV-2 Wuhan-1 strain RBD and N coupled beads (Bio-Rad, Cat Nos. 12015406 and 12014773), and Bio-Plex Pro Human IgA detection antibody (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... 30 second at 72°C and a final extension of 7 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). Five μl of PCR product was visualized on a non-denaturing 1% agarose gel with a 100bp ladder ...
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bioRxiv - Neuroscience 2021Quote: ... 40 min) and were subsequently transferred to nitrocellulose membranes for semi-dry immunoblotting (25 V, 1,3 A, 7 min, Bio-Rad®). Membranes were dried 5 min at 37°C and then incubated with 5% non-fat dried milk in Tris-buffered salineTween-20® (TBST containing 28 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: Two micrograms of each RNA sample was loaded on 10% polyacrylamide gels containing 7 M urea or loaded onto 10% Criterion TBE-urea precast gels (Bio-Rad) and electrophoresed at 85 V ...
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bioRxiv - Pathology 2021Quote: ... Real-time PCR was performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo-Fisher) using the iTaq Universal SYBR green Supermix (Bio-Rad). The following primer sequences were used ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred electrophoretically at a constant voltage of 25 V for 7 min using a Trans-Blot Turbo Transfer System (Bio-Rad). After transfer ...
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bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
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bioRxiv - Immunology 2021Quote: ... P2 was dialyzed against 1% glycine containing 2% ampholytes pH 3-10 (first run) or pH 5-7 (second run) and then loaded onto a liquid isoelectric focusing system (Rotofor, Bio-Rad). Individual fractions were harvested and their pH and absorbance at 280nm were measured ...
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bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 30 °C for 7 days and imaged daily using a gel documentation system (Gel Doc™ XR+, BIORAD). When needed ...
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bioRxiv - Biochemistry 2020Quote: ... the buffer of the purified DNAJB6 was exchanged into 10 mM ammonium acetate pH 7 solution using Micro Biospin P6 centrifuge columns (Bio-Rad) and protein concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... RNA was transferred to a nylon membrane (Amersham Hybond-XL) at 23 V for 7 minutes using a Trans-Blot Turbo Transfer System (Bio-Rad). Following transfer ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were resolved on 7-15% gradient polyacrylamide (SDS-PAGE) gels and transferred to nitrocellulose membranes via Trans Blot Turbo (Bio-Rad). Membranes were then blocked with 5% milk in Tris-buffered saline and 0.05% Tween 20 (TBST ...
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bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
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bioRxiv - Biochemistry 2022Quote: ... 1:10 000 dilution for N-terminal Parkin Ab) was incubated one hour at RT and protein visualized using ClarityTM Western ECL Substrate (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... and the quantity of radiolabel included into the acid insoluble fraction was analysed using Quantity One software (Bio-Rad). The quantitative data were analysed using MS Excel 2010 and presented on histograms as the Mean ± SD.
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bioRxiv - Microbiology 2021Quote: ... PCR reaction mixtures were as follows: 5.5 μl One-Step RT-ddPCR Advanced Kit for Probes Supermix (no dUTP’s) (Bio-Rad), 2.2 μl reverse transcriptase ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed at 150V for one hour through 7.5% or 4-20% mini-PROTEAN TGX gels (Bio-Rad) and blotted at 300 mA for one hour onto PVDF membranes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kit and BioRad chemiluminiscence machine scanner.The densitometry analysis of immunoblot images was performed using quantity One Software(Bio-Rad). Densitometry values were normalzed to the loading control(Gapdh or Actin ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR reactions were set up using the iTaq Universal Sybr Green One-Step RT-PCR kit (Biorad) according to manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was then quantified by qRT-PCR using the iTaq™ Universal Probes One-Step Kit (BIO-RAD) according to manufacturer’s protocol using a forward primer (5’-CCGCTGCCCAACACAAG-3’) ...
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bioRxiv - Microbiology 2021Quote: ... and amplicons were generated via qRT-PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD) according to manufacture protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... an iScript reverse transcription kit was used to convert one microgram of RNA to cDNA (Bio-Rad, Hercules, CA). No reverse transcriptase controls were included ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-associated fluorescence intensities (arbitrary units, AU) were analyzed using Quantity One image analysis software (BioRad, Hercules, CA, USA). Gels were stained with Colloidal Coomassie Brilliant Blue G250 and scanned.
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bioRxiv - Microbiology 2021Quote: Pictures were obtained with Bio-Rad Quantity One software implemented in a Bio-Rad GelDoc Imaging system (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2019Quote: ... The extracted RNA was amplified by iTaq universal SYBR Green one-step PCR kit (Bio-Rad, Hercules, CA, USA) and 10μM primers (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... One aliquot from each time point was analyzed by SDS/PAGE using a Criterion 16.5% TRIS/TRICINE gel (BioRad) along with [14C]fIX -18/+41 standards ...
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bioRxiv - Genetics 2020Quote: ... digital droplet PCR (ddPCR) was performed using the One-step RT-ddPCR advanced kit for probes (Bio-Rad, #1864022). The final 20 μL reaction mix contained ...
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bioRxiv - Pathology 2021Quote: ... One gel was used for Western blotting and the other was stained with SYPRO Ruby gel stain (Bio-Rad) for visualizing total protein.
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bioRxiv - Cancer Biology 2021Quote: ... One microgram of extracted RNA was reverse transcribed into cDNA using iScript cDNA Synthesis Kit (BIO-RAD, Cat #1708890). Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward ...
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bioRxiv - Plant Biology 2022Quote: One microgram of RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative real-time PCR analysis was performed on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... Purified RNA was analyzed by TaqMan qRT-PCR one-step RT-PCR kit for probes (Bio-Rad laboratories, Italy) with a TaqMan RT-PCR instrument (CFX384 real time system ...
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bioRxiv - Microbiology 2022Quote: ... Blots were photographed and revealed taking one picture every second for 10 seconds using ChemiDoc Imaging System (Bio-Rad). Blots were then annotated ...
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bioRxiv - Microbiology 2023Quote: ... using manufacturers protocol and imaged on a ChemiDoc XRS Imaging System with Quantity One imaging software (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2024Quote: ... using 11 μL undiluted RNA and the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Cat# 1864021). Droplets were generated ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA titers and infections were assessed via qRT-PCR using an iTaq Universal Probes One-Step Kit (BioRad) in a 20 µl sample volume ...
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bioRxiv - Microbiology 2019Quote: ... 250 µl packed cells were combined with 250 µl of 1 × Cytomix in a 2 mm transfection cuvette (Bio-Rad Laboratories). The precipitated and resuspended DNA was added to the cell suspension in the cuvette ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with 1:200 rabbit-anti NF-B p65 polyclonal antibody (Pierce) (2 h) and 1:5000 Goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) (1 h) ...
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bioRxiv - Molecular Biology 2020Quote: ... for 2 h at room temperature and a HRP-conjugated goat anti-rabbit secondary antibody (Bio-Rad 1706515; 1:3000 dilution) for 1 h at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were resolved electrophoretically on ethidium bromide stained-2% agarose gel immersed in 1× TAE and visualized with Biorad Gel Doc XR instrument (Biorad, USA).
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bioRxiv - Biophysics 2021Quote: ... The DDM/CHS detergent was then removed by overnight incubation with Bio-Beads SM-2 (15 mg per 1 ml protein/detergent/amphipols mixture, Bio-Rad) at 4 °C ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
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bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
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bioRxiv - Biochemistry 2022Quote: An equivalent volume of ∼150 µg FBS was first diluted 1:2 with loading buffer (consisting of 2X Laemmli Sample Buffer (#1610737, Bio-Rad) plus a 5% (v/v ...