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Citations for Bio-Rad :
6401 - 6450 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR analysis were performed with iQ-SYBR Green Supermix kit in an iCycler iQ5 system following the instructions of the manufacturer (Bio-Rad, USA). The relative quantifications of mRNA levels were performed using the cycle threshold (Ct ...
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bioRxiv - Synthetic Biology 2022Quote: ... #FABRK 001-1) and 400 ng of cDNA was synthesized using the iScript™ Select cDNA Synthesis Kit (Bio-Rad Laboratories, #1708897) on a Mastercycler® nexus PCR Thermal Cycler (Eppendorf) ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Germany) and 1 μg of total mRNA was translated to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the instructions.
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bioRxiv - Biochemistry 2023Quote: ... The determination of casein concentration by the Lowry method was performed using the DC™ protein assay kit purchased from Bio-Rad Laboratories (California ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized with 1 µg of total RNA by using the iScript cDNA Synthesis Kit (Bio-Rad, Cat# 1708891). cDNA was diluted 20 times and real-time quantitative PCR was performed by using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Visualization was performed using a horseradish peroxidase sensitive ECL western blotting detection kit (SuperSignal West Pico PLUS Chemiluminescent Substrate, ThermoScientific) and the result was recorded using Chemidoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... Membranes were developed using the SuperSignal West Pico Chemiluminescent Substrate kit (Fisher #PI34578) and visualized on a ChemiDoc™ Touch Gel Imaging System (BioRad #1708370).
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bioRxiv - Bioengineering 2023Quote: ... We then added 80 μL of X1 streptavidin phycoerythrin SA-PE (Bio-Plex Pro Reagent Kit III #171304090M, BioRad, Hercules, CA, USA) and incubated it for another 20 minutes ...
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bioRxiv - Physiology 2023Quote: ... One hundred nanograms of RNA was used as input to synthesize cDNA using the iScript cDNA synthesis kit (BioRad, Hercules, CA, USA). cDNA templates were used for quantitative real-time PCR ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 4°C at 20,160 x g for 10 minutes and supernatants were collected for protein measurement using the DC Protein Assay Kit (Bio-Rad, 5000111). Equal amounts of protein were loaded to run the gels (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Once ready for testing we quantified the amount of mitochondria from each isolation using Bio-Rad Protein Assay Kit (Bio-Rad, #5000001), plated 3µg/50µl/well onto a 96 well plate with all animals having 6-8 technical replicates (101085-004 ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified with the RC DC Protein assay Kit II (cat. No. 5000122; Bio-Rad Labortaories Inc, California, USA). After protein concentration assessment ...
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bioRxiv - Microbiology 2024Quote: qRT-PCR analysis was performed on three major selected genes and validated as follows: The first cDNA strand was prepared using the iScript™ cDNA synthesis kit (Bio-Rad). qRT-PCR was performed using the SsoFast™ EvaGreen® supermix kit (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... using cDNA generated from 0.1-2 ng total RNA with the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad catalog # 1864021) according to the manufacturer’s protocol with reverse transcription at 42°C and annealing/extension at 60°C.
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Plant Biology 2024Quote: ... The transcripts of defense marker genes were quantified by using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD, #1725150) with 40 cycles of 1 s at 95°C and 30 s at 60°C.
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bioRxiv - Microbiology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was performed with SYBR green fluorescent PCR kit (Cat. # 208054) and ran on a CFX96 RealTime System (Bio-Rad Laboratories). The relative expression level was calculated as 2(-ΔΔCT) ...
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bioRxiv - Molecular Biology 2024Quote: ... CD45-APC and Bu1-PerCP-Cy5.5 were generated by conjugation of the antibody with the respective Lynx Rapid conjugation kit (Bio-Rad, Feldkirchen, Germany). Samples were analyzed on a FACS Canto II and FlowJo 10.9 software (BD ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using the iScript™ cDNA Synthesis Kit according to the manufacturer’s instructions (Cat#1708890, Bio-Rad Laboratories, Hercules, CA). Quantitative real-time PCR was conducted using SYBR Green probes and measured using the QuantStudio using the following PCR settings ...
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bioRxiv - Immunology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Neuroscience 2024Quote: ... each RNA sample was reverse-transcribed and quantitative real-time PCR was performed simultaneously in a single step using iTaq Universal SYBR Green One-step kit (Bio-Rad, #1725150), in the Lightcycler instrument (Roche) ...
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bioRxiv - Physiology 2024Quote: ... and about 500 ng of total RNA was reverse transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (#1725035, Bio-Rad, Mississauga, ON, Canada). Real time (RT ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesised from 1 μg of total RNA from each sample following the manufacturer’s instructions of the iScript™ cDNA synthesis kit (Biorad, California, USA). For RNA-Seq validation by RT-qPCR ...
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bioRxiv - Genomics 2024Quote: RT-qPCR reactions of 12.5 ng DNase-treated RNA were prepared using the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) and run on the Bio-Rad CFX Opus 384 Real-Time PCR System ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of total RNA was converted into cDNA using an iScript™ gDNA Clear cDNA Synthesis Kit (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The SDS-PAGE gels were blotted onto PVDF membranes using the Trans-Blot Turbo Transfer System in combination with the Trans-Blot® Turbo™ RTA Midi PVDF Transfer Kit from Biorad and the SDS PAGE Transfer Buffer according to the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... 1ug of RNA from each sample was then used for cDNA synthesis using the iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Real-time quantitative PCR was then performed in quadruplicate using a [check model] and detected using SYBR Green (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was developed with an ECL chemiluminescence detection kit and visualized with the Gel documentation system (Biorad ChemiDoc MP Imaging System). The protein level on the blot was quantified using ImageJ software.
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bioRxiv - Cancer Biology 2024Quote: ... About 1µg of total RNA was reverse transcribed to cDNA with the iScript cDNA Synthesis kit (Bio-Rad Laboratories, Hercules, CA; #1708891) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were then resuspended in PBS and 2X Laemmli Sample Buffer (Bio-Rad, Cat. No. 1610737) containing 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed in cold PBS and lysed in 2x Laemmli Sample Buffer (Bio-Rad Laboratories, 1,610,737) supplemented with DDT ...
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bioRxiv - Immunology 2021Quote: ... Total cell lysate was extracted using RIPA buffer and quantified using the DC protein assay (Bio-Rad). 15–30 μg of protein was loaded and run on 4%–12% SDS PAGE gels ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD) at 20 V for 30 min ...
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bioRxiv - Physiology 2022Quote: ... and viability confirmed to be >85% using a TC20™ Automated Cell Counter (BioRad, Cat. No. 1450102).
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bioRxiv - Cell Biology 2022Quote: Unstained SDS gels were transferred to a nitrocellulose membrane using a semi-dry transfer cell (Bio-rad) for 2 hours at 12 volts ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then transferred onto nitrocellulose (NC) membrane using an electrophoretic transfer cell (Bio-Rad Laboratories, USA) at RT (28) ...
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bioRxiv - Bioengineering 2020Quote: ... 1D separation was run according to the O’Farrell protocol63 (adapted for Bio-Rad Mini-PROTEAN Tube Cell). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... 2D separation was run according to the Laemmli protocol64 (adapted for Bio-Rad Mini-PROTEAN Tetra Cell). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then pulsed with 1.8 kV in a 1-mm-gap cuvette (Bio-Rad, USA).
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bioRxiv - Genetics 2020Quote: ... and cell lysates were analyzed by SDS-PAGE using 4–20% gradient Tis-HCl gels (BioRad, #3450034). Proteins were transferred onto 0.2-μm nitrocellulose membrane ...
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bioRxiv - Bioengineering 2021Quote: ADMSC metabolic activity was tracked over 14 days using the alamarBlue® Cell Viability Assay (Bio-Rad). Media was removed from samples of S-50 or P-0 (n = 5 each) ...
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bioRxiv - Cancer Biology 2020Quote: Cell extracts were harvested by direct addition of 2x SDS sample buffer (1610747, BioRad Laboratories, Alcobendas, Spain) containing 100 mM DTT to the culture plate and incubation on ice for 10 minutes ...
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bioRxiv - Physiology 2021Quote: ... and proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 70 volts for 2 hours at 4 °C ...