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Citations for Bio-Rad :
6401 - 6450 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... #FABRK 001-1) and 400 ng of cDNA was synthesized using the iScript™ Select cDNA Synthesis Kit (Bio-Rad Laboratories, #1708897) on a Mastercycler® nexus PCR Thermal Cycler (Eppendorf) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantified by nanodrop and cDNA was produced from 1□μg of purified RNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories). qPCR reactions were performed using the SsoFast Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were run on a 1% Agarose gel containing .08% ethidium bromide and visualized using the ChemiDoc imaging system (BioRad, Hercules, USA). PCR amplicons were sanger sequenced (Psomagen ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Neuroscience 2022Quote: ... The diluted cDNA (1:10) was used as a template for real-time PCR (Bio-Rad CFX 96™ Real-Time system) with an SYBR® Green Supermix mixture following the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Germany) and 1 μg of total mRNA was translated to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the instructions.
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was diluted to 1:200 (snRNA KD) or 1:25 (motor neurons and EXOSC10 KD) and qRT-PCR was performed with Ssofast EvaGreen (Bio-Rad, 1725201) with the following cycling conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% Acrylamide:Bis Acrylamide) for 1 hour and transferred to a nitrocellulose membrane (0.45µm) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Genetics 2024Quote: ... at 75 V for 15 min and 100 V for 1 hr 15 min using 1X Tris/Glycine/SDS running buffer (BioRad Cat. # 1610772). Following electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates were boiled in loading buffer [1:9 ratio of 2-mercaptoethanol:4x Laemmli Sample Buffer (Cat.#1610747; Bio-Rad Laboratories)] for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were detected using chemiluminescence using horseradish peroxidase–conjugated secondary antibodies of anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) origin and enhanced chemiluminescence substrate (Thermo Scientific # 32106) ...
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bioRxiv - Immunology 2024Quote: ... A 3.3 mm DBS punch from each sample was combined with 250 µl elution buffer (Tris-buffered saline, 1% casein blocker) (Bio-Rad Laboratories) in a 96 well plate ...
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bioRxiv - Developmental Biology 2023Quote: ... Transfer buffer was prepared as a 1X final solution by mixing 7:2:1 of ddH2O: methanol: 10X Tris/Glycine Transfer Buffer (Bio-Rad #1610734). Following transfer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... The reactions were stopped with 1 M H2SO4 after 20 min and the absorbance was measured in a microplate reader (Benchmark; Bio-Rad Laboratories) at 490 nm.
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bioRxiv - Molecular Biology 2023Quote: ... and 95 % humidity to let them attach and recover to a concentration of 1×106 cells/m to 20 million cells counted using an automated Cell Counter System (Countess TC20, Biorad, Hercules, CA) which assessed cell viability via trypan blue exclusion ...
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bioRxiv - Molecular Biology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, USA). The detection limit was calculated to be 1000 copies per reaction ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 15,000 x g for 1 min in a tabletop centrifuge to pellet cell debris and the supernatant was diluted 1:10 in sterile ddH2O for a 96-well microplate Bradford microassay (Bio-Rad 5000201) to estimate the amount of leaked protein ...
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bioRxiv - Bioengineering 2023Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... wre incubated for 1 h at RT and membranes were revealed by using ECL reagents with the ChemiDoc imager (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2023Quote: ... cDNA was diluted 1:10 in nuclease free water prior to qPCR analysis using a CFX384 real-time PCR system (Bio-Rad, USA). Reaction mixtures containing 5 μl PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) synthesis was performed using 1 μg of total RNA with the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The genes encoding for the zonula occludens-1 (Zo-1) ...
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bioRxiv - Neuroscience 2023Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS solution with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
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bioRxiv - Physiology 2023Quote: ... The blots were washed four times with 0.1% tween TBS before being incubated for 1 h at room temperature with goat Anti-rabbit IgG HRP conjugated secondary antibody (Bio-Rad, USA) in 0.1% tween TBS buffer containing 3% BSA (Bovogen ...
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bioRxiv - Plant Biology 2023Quote: ... The 1st strand cDNA was synthesised from 1 µg of RNA for each sample using iScript™ cDNA Synthesis Kit (Bio-Rad). This cDNA was then used as the template in qRT-PCR reaction mixture after 10 times dilution ...
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bioRxiv - Immunology 2023Quote: ... qPCR reactions were conducted using 1 μl of cDNA product in 10 μl total reaction volume using Itaq Universal SYBR green (Bio-Rad, #1725121) with primers at a concentration of 500nM ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody incubation was done at room temperature for 1h (HRP-conjugated goat anti-rabbit IgG, 1:2000 dilution, Bio-Rad, 403005). Membranes were washed with Tris-buffered saline containing 0.05% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... before injecting into 1 mL Hitrap Heparin HP column pre-equilibrated with Wash Buffer C operated in the BioLogic DuoFlow™ FPLC system (Bio-rad). The protein was eluted from the column by running a gradient flow rate that exchanges Wash Buffer C and Elution Buffer D (2000 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of inflammatory cytokines were quantified using Bio-Plex Pro Human Inflammation Panel 1 37-plex (#171AL001M) magnetic bead-based assay (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were detected using chemiluminescence using HRP conjugated secondary antibodies of either anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) or anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized with 1 µg of total RNA by using the iScript cDNA Synthesis Kit (Bio-Rad, Cat# 1708891). cDNA was diluted 20 times and real-time quantitative PCR was performed by using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction collection was performed using a Masterflex L/S easy-load II (Cole Parmer, 77200-50) running at approx 1 mL/min and BioFrac™ fraction collector (Bio-Rad) with a fraction time window of 2 min ...
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bioRxiv - Microbiology 2023Quote: ... before destaining with Tris buffered saline Tween-20 (TBST) and incubation for 1 hour with blocking buffer (TBST with 50 g/L blocking agent (Bio-Rad, 1706404)) ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated for 1 h in water with 3X GelRed (Phenix Research Products) and imaged on ChemiDoc XRS imaging system (Bio-Rad Laboratories) and Quantity One imaging software (Bio-Rad Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... a total of 20 µL of 1 µg/µL plasmid DNA was combined with 50 µL of gold microparticles (1 µm, 50 mg/ml, suspended in 50% (v/v) sterile glycerol) (#1652263, Bio-Rad, USA), 100 µL 2.5 M CaCl2 and 40 µL 0.1 M spermidine (S0266 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane using the iBlot 2 device and membranes were blocked for 1 hour at RT in 5% (w/v) Blotting Grade Blocker/PBS (Biorad, #170-6404). GCase was detected using rb mAb to hGBA antibody (abcam ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0, 150 mM NaCl, 1% NP-40 [United States Biological, 19628], 0.5% deoxycholate [Sigma-Aldrich, D6750], 0.1% SDS [Bio-Rad, 1610302]) containing protease inhibitor cocktail and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Immunology 2024Quote: RNA was isolated (ZymoResearch R2050) from the infected Hsu cells at various passages and 1 μg of RNA was used to prepare cDNA (BioRad 170-8891). The cDNA was used as the template for various PCR reactions targeting the 4 genes of interest - E ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.5 μL of forward and reverse primers (see Supplementary Table 1 for primer sequences) for the genes of interest were combined in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a CFX384 Real Time PCR Detection System using the following program ...
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bioRxiv - Developmental Biology 2024Quote: ... see Supplementary Table 1 for primer sequences) and diluted to 20 μL with nuclease free water in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a Bio-Rad CFX384 Real Time PCR Detection System with the following program ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then rinsed 3 times with PBS to ensure the complete removal of glutaraldehyde and blocked for 1 h in blocking buffer (5% milk (Bio-Rad #1706404) in Tris-Buffered Saline (TBS ...
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bioRxiv - Plant Biology 2024Quote: ... and 1 µl of cDNA was used as a template for qRT-PCR using iQTM SYBR® Green Supermix (BIO-RAD, USA) on a CFX96TM Real-Time PCR machine (BIO-RAD ...
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bioRxiv - Immunology 2024Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The stain-free gel was UV-activated for 1 minute and imaged for total protein (BioRad Chemi Doc XR+, Hercules, CA, USA) with ImageLab software (BioRad Image Lab v6) ...