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Citations for Bio-Rad :
6251 - 6300 of 9194 citations for Glutathione Fluorescent 384 Well Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Purified polar flagellins (2 µg) were separated by 12% SDS-PAGE as described previously [33] and stained with Coomassie Blue (BioRad, Madrid, Spain), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The relative number of cells in each well was evaluated after solubilization in 40 µL of 2% Triton X-100 by measuring absorbance at 595 nm in a microplate reader (Bio-Rad Laboratories). All determinations were carried out in triplicate.
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8 μm cryostat sections of C51 and F9 tumors stained with IgG (L19)-FITC (final concentration 2 μg/mL) and detected with a Rabbit anti-FITC (Bio-Rad; 4510-7804) and Donkey anti-Rabbit AlexaFluor 488 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 hours at room temperature with HRP-conjugated rabbit secondary antibody (Bio-Rad, USA, 170-6515; RRID: AB_11125142) for NALCN detection and HRP-conjugated mouse secondary antibody (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are listed in Supplemental table 2 Immunoreactive proteins were visualized with Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA, USA). The UVP ChemStudio (Analytik Jena US LLC ...
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bioRxiv - Cell Biology 2021Quote: ... were amplified using Phusion High-Fidelity PCR MasterMix (primer sequences are listed in Additional File 2) and a C1000 thermocycler (Bio-Rad Life Science). The peak sequences were amplified for 98 °C for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with specific primers (Supplementary Table 2) using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) and CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... and Reverse Transcription (RT) was performed with 2 µg RNA per 40 µl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
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bioRxiv - Biophysics 2022Quote: ... the samples were cooled on ice for 10 min followed by the addition of 80 mg Bio-beads SM-2 Resin (Bio-Rad, Hercules, CA), then rotated at 4°C overnight ...
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bioRxiv - Biophysics 2022Quote: The purified proteins of V2HeR3 were reconstituted into a mixture of POPE and POPG membranes (molar ratio = 3:1) with a protein-to-lipid molar ratio of 1:20 by removing DDM using Bio-Beads (SM-2; Bio-Rad, CA, USA). The reconstituted samples were washed three times with 1 mM NaCl and 2 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2022Quote: ... ddPCR was used to detect DNA of SP by using a QX100 Droplet Digital PCR System and 2× ddPCR Supermix (Bio-Rad, California, CA) with protocols developed in our previous studies 48-57 ...
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bioRxiv - Cell Biology 2022Quote: ... in 4°C for 2-4 hours and the beads were then isolated and eluted through a Bio-Spin® Chromatography Column (Bio-Rad, #7326008) with elution buffer (5 0mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate/bead mixtures were incubated at 4°C on a rotating platform for 2 hrs and subsequently poured into Poly-Prep® Chromatography Columns (Bio-Rad #7311550). Unbound fractions were collected through the columns and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... and Reverse Transcription (RT) was performed with 2 μg RNA per 40 μl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
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bioRxiv - Genetics 2022Quote: ... a small implant of the thallus of interest (0.5 cm2) was set in 2 mL tube containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Cell Biology 2020Quote: ... fixed in 4% paraformaldehyde for 2 h at 4 °C and embedded in PBS containing 3% low-melting point agarose (Bio-Rad, California, USA). The agarose gels containing the fixed organoids were processed in a standard automated tissue histology processor ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... The resulting mixtures were dialyzed (20 kDa cutoff) for ∼18h at 4°C in the dark against 250 volumes of FB160M1 containing 2 g BioBeads SM2 (Bio-Rad, Hercules, CA) per 2 mL of RPL mixture ...
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bioRxiv - Immunology 2020Quote: ... The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories, USA) with 2 mM ATP (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 95°C for 2 min and amplified for 24 cycles (95°C 15 seconds, 60°C, 4 min) on T100 PCR instrument (Biorad, Hercules, CA, USA). At the end of this process ...
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bioRxiv - Molecular Biology 2021Quote: ... and then liposomes were formed by the step-wise removal of pentaethylene glycol monodecyl ether by five additions at 20 min intervals of 60 mg Bio-Beads SM-2 (Bio-Rad, Watford, UK) with gentle mixing at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Round spermatids were sorted based on 1C DNA content and size using a 2-laser sorter (BioRad S3e with 488nm and 561nm lasers) gated on single cells as previously described (Lesch et al. ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were resolved by SDS-PAGE and transferred to a PVDF membrane by tank blotting at 150 mA for 2 h using the Miniprotean III System from Bio-Rad (Hercules, USA). The membrane was blocked for 1 h with 5% skim milk in PBS containing 0.05% Tween-20 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38) before running on a CFX96TM real-time PCR cycler (BioRad, Hercules, CA, USA) under cycle conditions of 42°C for 5 min ...
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bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Plant Biology 2022Quote: ... 50 mg of powder was transferred into a 1.5 mL microcentrifuge tube with 150 ul of 2 x Laemmli buffer (Bio-Rad, Hercules, CA, USA). The sample was immediately vortexed for 1 min followed by incubation at 70°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... The results were obtained from channels 1 (FAM) and 2 (HEX/VIC) and analysed with QuantaSoft™ Analysis Pro 1.0 (Bio-Rad Laboratories, Inc). All ddPCR reactions were prepared with QX200™ ddPCR™ EvaGreen® Supermix (2x).
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bioRxiv - Molecular Biology 2024Quote: ... The assembled products were mixed with electrocompetent cells and transformed into electrocompetent cells in a 2-mm Gene Pulser cuvette (Bio-Rad, 165-2086) using an electroporation instrument (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... each lysate was diluted at a 1:1 ratio with a solution of 2× Laemmli sample buffer (#1610737EDU, Bio-Rad, Hercules, CA, USA), containing 5% (v/v ...
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bioRxiv - Immunology 2023Quote: ... The amplified products were analyzed on a 2% agarose gel stained with SYBR safe and imaged using the Gel-documentation system (Bio-Rad, Hercules, CA).
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bioRxiv - Cell Biology 2023Quote: RT-PCR was performed after generating first-strand cDNAs from 2 µg of total RNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA), as recommended by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... Twenty µg (islet sample) or 2 µg (mouse liver sample: positive control) was mixed with 10uL of Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and denatured at 95°C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: mMEC and hMEC samples were directly homogenized using 2x Laemmli Sample Buffer and supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Proteins were separated by electrophoresis on a polyacrylamide gradient gel (BioRad Hercules ...
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bioRxiv - Biochemistry 2023Quote: ... plates were stained with primuline (0.05% in acetone/water, 8/2; v/v) and photographed under long-wave UV light (Biorad ChemiDoc XRS Imaging System).
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (0.2 µm pore size) using the Trans-Blot® Turbo™ semi-dry transfer system (7 min mixed MW MIDI program) (Bio-Rad). After blocking in a 2.5% (w/v ...
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bioRxiv - Physiology 2024Quote: ... and loaded (crude homogenate: 10 µg per lane; membrane: 2 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2024Quote: ... Expression of Dicer-Like 2 (DCL2) (Glyma.09g025300) and Argonaute 1 (AGO1) (Glyma.09g167100) was assessed using iTaq Universal SYBR Green Supermix (BioRad, Hercules, CA, USA). To quantify F ...
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bioRxiv - Physiology 2024Quote: ... Ten μg (final volume 2 μl for all samples) of serum proteins were loaded onto a nitrocellulose membrane (Bio-rad Laboratories, CA, USA). Then ...
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
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bioRxiv - Plant Biology 2020Quote: ... Complementary DNA (cDNA) was synthesized using the iScript cDNA synthesis kit (Bio-Rad) with primers (Supporting Information Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized by reverse transcription using the iScript kit (BioRad, Stockholm, Sweden). Transcripts of interest were analyzed using the QuantStudio 5 real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories Inc.). qRT-PCR was performed using Power SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed with the iScript cDNA Synthesis kit (Biorad, Munich, Germany) and cDNA was analyzed by qPCR using the SsoFast Eva Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... First strand cDNA was made using the iScript cDNA Synthesis Kit (Bio-Rad). CT values from quantitative PCR were analyzed with the delta delta CT method using 18S as an internal control (Livak and Schmittgen ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein amount was determined (DC Protein Assay kit, Bio-Rad, Hercules, CA) and samples equalized with ddH2O and NuPAGE LDS 4x Sample Buffer (Invitrogen ...