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Citations for Bio-Rad :
6101 - 6150 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RNA concentration was measured using a NanoDrop spectrophotometer and 1 µg of RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). Primers sequences were obtained from Harvard primerbank (sequences are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 3-4 μg of total RNA was reverse transcribed using the iScript advanced cDNA synthesis kit (Bio-Rad). Quantitative PCR of target genes (Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Genetics 2022Quote: Approximately 500 ng of total RNA was used to synthesize cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The HRM analysis was done according to the protocol provided by the Precision Melt Supermix Kit (Bio-Rad; Catalog #172-5112). The following oligonucleotides were used for the HRM analysis ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription of 500ng of total RNA was performed using the iScript cDNA synthesis kit (Bio-Rad Laboratories, Inc, California, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... methanol in 1X TBE buffer with 10% (v/v) acetic acid) and RNA was visualised using the Silver Stain Plus Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1µg of each total RNA sample was used to synthesize cDNA using the i-Script cDNA synthesis kit (Bio-Rad, USA). The real-time PCR was performed using a 20µl reaction volume on 96-well plates by using i-Taq Syber Green (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Zoology 2022Quote: ... sfRNA and 3’UTR were quantified together by RT-qPCR using the iTaq Universal Sybr green one-step kit (Bio-Rad) with primers previously designed [26] ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were separated in 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane using a Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit (Bio-Rad). Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Microbiology 2022Quote: Cell pellets from 2 mL overnight cultures were used to create gel plugs using the CHEF Bacterial Genomic DNA Plug Kit (Bio-rad). DNA was digested with I-SceI ...
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bioRxiv - Microbiology 2022Quote: ... β-lactamase activities were normalized relative to total protein concentration measured using Pierce Coomassie Plus Assay Kit (Bio-Rad, CA, USA) to obtain the enzyme activity in units pr ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We then performed quantitative polymerase chain reaction (qPCR) using 2 ng of complementary DNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... RNA quantity and quality was assessed using a NanoDrop spectrophotometer and cDNA was synthesized using an iScript cDNA Synthesis Kit (Biorad, 1708891) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (1 μg) was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170±8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... in SDS running buffer then blotted onto nitrocellulose membranes through Trans-Blot Turbo RTA Mini 0.2 µm Nitrocellulose Transfer Kit (Bio-Rad #1704270). Membranes were hybridized with 1:1000 dilution of the anti-Arm primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription to create complementary DNA (cDNA) with the iScript cDNA Synthesis Kit (Bio-Rad) according to manufacturer’s instructions in a total volume of 20 μl ...
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bioRxiv - Immunology 2023Quote: ... The concentration of the isolated RNA was determined using Nanodrop and 250ng RNA was converted into cDNA using the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were developed using the ECL kit (Seven, Beijing, China) and detected using the ChemiDoc imaging system (Bio-Rad, California, USA).
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bioRxiv - Microbiology 2022Quote: ... The blots were then washed three times by PBST and then visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) by a ChemiDoc MP Imaging System (Bio-Rad).
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA Synthesis Kit protocol (Bio-Rad Laboratories Inc.; Hercules, USA), and the concentration was determined using a Qubit flex fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaq™ universal SYBR® Green One-Step kit (Bio-Rad): 95 ºC for 5 min ...
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bioRxiv - Immunology 2023Quote: ... we developed a digital droplet RT-PCR (RT-ddPCR) employing the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad) and the QX200 Droplet Digital PCR System (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from whole Drosophila animals and converted to cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad, cat#1708891). Primers for qPCR were designed using IDT PrimerQuest (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentrations in each tube were measured by Bradford assay using Quick Start Bradford protein assay kit (Bio-Rad). An equal amount of each sample (30-35 μl) ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Biorad, #1725035; Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration of the supernatant was measured using the detergent compatible (DC Lowry) protein assay kit according to the manufacturer′s directions (Bio-Rad). For each sample ...
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bioRxiv - Genetics 2024Quote: 500 ng of RNA was converted to cDNA using the iScript cDNA synthesis kit (#1708891, Bio-Rad, Hercules, CA, United States) containing random hexamers according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin signal was detected and quantified by chemiluminescence according to standard LightShift Chemiluminescent RNA EMSA Kit protocol using a ChemiDoc™ Touch Imaging System (BioRad).
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bioRxiv - Genetics 2024Quote: ... and cDNA was generated from 0.5 micrograms of purified RNA from each sample using an iScript cDNA Synthesis Kit (Bio-Rad #1708890). qPCR was conducted using iTaq Universal SYBR Green Supermix (Bio-Rad #1725151) ...
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bioRxiv - Biophysics 2024Quote: ... A luminol-based chemiluminescence assay kit (Chemi-Lumi One L, Nacalai tesque) and chemiluminescence detection system (ChemiDoc Touch MP imaging system, Bio-Rad) were used to detect chemiluminescence signals.
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... and the units were converted to molar ellipticity based on protein concentration measured in the sample by the DC protein assay kit (Bio-Rad) using WT USP14 as standard ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Microbiology 2024Quote: ... the blots were washed four times again by PBST and visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) through a ChemiDoc MP Imaging System (Bio-Rad). To examine the S glycoprotein packaging efficiency ...
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bioRxiv - Developmental Biology 2024Quote: 100 ng of enriched small RNA fraction was used for generated cDNA following iScript Select cDNA Synthesis Kit (#708896, Bio-Rad) following manufacture’s protocol and the specific primer 5′-GCAGGTCCAGTTTTTTTTTTTTTTTCTACCCC-3′ (Supp ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA for measuring primary-miR430 was generated using 200 ng of total purified RNA following iScript cDNA synthesis kit (#1708890, Bio-Rad) manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of total RNA was used for a reverse transcriptase reaction using iScript cDNA Synthesis Kit (Catalog #170-8891; Bio-Rad). RT-qPCR analysis was conducted using iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse-transcription products of samples were amplified by the One-step gDNA Removal and cDNA Synthesis kit (TransGen Biotech) using the SYBR Green SuperMix (Bio-Rad). Primers used for RT-PCR assays are listed in Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was DNase treated and reverse transcribed using the iScript gDNA clear cDNA Synthesis kit (BioRad, 1725035). CDSs were then amplified with sequence specific primers annealing at the start (forward ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was purified from the lysates and immediately converted into complementary DNA (cDNA) using a reverse transcription kit (iScript Reverse Transcription Supermix, Bio-Rad). The resulting cDNA samples were stored at -80°C until PCR analysis ...
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bioRxiv - Microbiology 2024Quote: ... was performed using the diastarTM onestep multiplex qRT-PCR kit (Solgent, South Korea) and Bio-Rad CFX Maestro 2.3 system (Bio-Rad, USA) with the following specific primers ...
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bioRxiv - Plant Biology 2024Quote: ... The biotin-labelled DNA probes were detected using the Chemiluminescent Nucleic Acid Detection Module Kit (ThermoScientificTM; USA) and pictured under BioRad Gel DocTM XR (BioRad; USA).