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Citations for Bio-Rad :
6001 - 6050 of 7813 citations for Cortisone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then exposed to the ECL substrate kit (Pierce 34850 and 34095) for 3 minutes before imaging with the ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was amplified using KAPA HiFi Hotstart ReadyMix PCR kit (Nippon Genetics) with T-100 Thermal Cycler (Biorad, Hercules, CA). The temperature profile of the reaction was 95 °C for 2 min ...
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... and cDNA was generated from 0.5 micrograms of purified RNA from each sample using an iScript cDNA Synthesis Kit (Bio-Rad #1708890). qPCR was conducted using iTaq Universal SYBR Green Supermix (Bio-Rad #1725151) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from cells using GeneJet RNA Purification kit (Thermo Fischer) and cDNA was synthesized using iScript Reverse Transcription Supermix (Bio-Rad), as previously described (27) ...
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bioRxiv - Microbiology 2024Quote: ... the blots were washed four times again by PBST and visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) through a ChemiDoc MP Imaging System (Bio-Rad). To examine the S glycoprotein packaging efficiency ...
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bioRxiv - Plant Biology 2024Quote: ... 5 μl supernatant was loaded onto 10% SDS polyacrylamide gels and the proteins were transferred onto PVDF membranes using the Trans-Blot Turbo RTA Transfer Kit (1704275, Bio-rad). Membranes were blocked in PBS-T (tablets 524650-1EA ...
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bioRxiv - Plant Biology 2024Quote: ... graminicola genomic DNA was quantified by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for the histone 3 (CgH3 ...
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bioRxiv - Plant Biology 2024Quote: ... The rRNA depleted was used immediately to prepare the metatranscriptome libraries using the SEQuoia complete RNAseq kit (Bio-Rad cat# 17005726) following the manufacturer’s procedures without any modifications ...
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bioRxiv - Plant Biology 2024Quote: ... higginsianum genomic DNA quantitation was performed by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for ChTrpC gene (Supplementary Table S1) ...
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bioRxiv - Biophysics 2024Quote: ... A luminol-based chemiluminescence assay kit (Chemi-Lumi One L, Nacalai tesque) and chemiluminescence detection system (ChemiDoc Touch MP imaging system, Bio-Rad) were used to detect chemiluminescence signals.
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... and the units were converted to molar ellipticity based on protein concentration measured in the sample by the DC protein assay kit (Bio-Rad) using WT USP14 as standard ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then blotted onto polyvinylidene difluoride (PVDF) membranes using Trans-Blot Turbo™ Transfer Starter System and Midi PVDF kit (Both are from Biorad). After transfer ...
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bioRxiv - Neuroscience 2024Quote: ... the gel was transferred to a LF PVDF membrane using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (BIO-RAD, 1704275). After blocking in 5% skim milk powder (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were heat at 95°C for 10 min and then separated by SDS-PAGE electrophoresis on 10% acrylamide gels (FastCast™ Acrylamide kit, BioRad) at 110V for 60 min ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Microbiology 2024Quote: RNA extracts were reverse transcribed into cDNA using an iScript™ Select cDNA Synthesis Kit (Bio-Rad, Marnes-la-Coquette, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative RT-PCRs (qRT-PCRs) were carried out in triplicate with three technical replicates with the SsoAdvanced Universal SYBR Green kit (Bio-Rad) on a CFX384 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Microbiology 2024Quote: We carried out reverse transcription with RNA prepared for transcriptome analysis using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA product was diluted 10-fold with water and we used 1Lμl per technical replicate for quantitative PCR analysis ...
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bioRxiv - Immunology 2024Quote: ... The following cytokines and chemokines were measured using a Bioplex Express Kit according to the manufacturer’s directions (Biorad, Hercules, CA, USA): GM-CSF ...
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bioRxiv - Microbiology 2024Quote: ... A total of 500 ng of resulting total RNA was used to synthesize cDNA via random priming utilizing the iScript™ cDNA Synthesis Kit (Biorad). Real-time qPCR was performed on QuantStudio 6 Pro (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... from colonic samples of DR3.IL17A-/- (n = 5) and DR3 mice (n = 5) were reverse transcribed to cDNA using the High-Capacity iScript cDNA synthesis kit (Bio-Rad). qPCR reactions were then carried out in triplicate using 50 ng of cDNA and the Applied Biosystems Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Genetics 2024Quote: 500 ng of RNA was converted to cDNA using the iScript cDNA synthesis kit (#1708891, Bio-Rad, Hercules, CA, United States) containing random hexamers according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... RNA concentrations were measured with the Little Lunatic (Unchained Labs) and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-rad). Primers for skeletal marker genes were designed using NCBI Primer-BLAST (Supplementary Table 3 ...
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bioRxiv - Developmental Biology 2024Quote: 100 ng of enriched small RNA fraction was used for generated cDNA following iScript Select cDNA Synthesis Kit (#708896, Bio-Rad) following manufacture’s protocol and the specific primer 5′-GCAGGTCCAGTTTTTTTTTTTTTTTCTACCCC-3′ (Supp ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA for measuring primary-miR430 was generated using 200 ng of total purified RNA following iScript cDNA synthesis kit (#1708890, Bio-Rad) manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin signal was detected and quantified by chemiluminescence according to standard LightShift Chemiluminescent RNA EMSA Kit protocol using a ChemiDoc™ Touch Imaging System (BioRad).
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bioRxiv - Plant Biology 2024Quote: ... One microgram of total RNA was used for a reverse transcriptase reaction using iScript cDNA Synthesis Kit (Catalog #170-8891; Bio-Rad). RT-qPCR analysis was conducted using iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... incubated with goat α-rabbit 2° antibody conjugated to HRP (1:5000) (Bio-Rad) in TBS-T at room temperature (1 h) ...
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bioRxiv - Cancer Biology 2021Quote: ... Plugs were loaded into a 1% certified low melt agarose gel (Bio-Rad, 1613112) in 0.5X TAE buffer with ladders (CHEF DNA Size Marker ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:2000)] proteins were detected using ClarityTM Western ECL Substrate (Bio-Rad. #170-5060). All western blots were repeated at least 3 times.
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bioRxiv - Cell Biology 2020Quote: ... and hFAB Rhodamine Anti-Tubulin Antibody (1:2500, Bio-Rad, Cat# 12004165, RRID: AB_2884950) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and 72°C for 1 min in a C100 thermocycler (BioRad, Hercules, CA, USA). 5 μL of this reaction was removed and used for a qPCR side reaction ...
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bioRxiv - Genomics 2020Quote: ... 1 μg of total RNA was reverse transcribed using iScript Reverse Transcriptase Supermix (BioRad). The relative levels of Firefly and Renilla luciferase transcripts in the sample were then quantified using Taqman qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Products were detected by running an 8% acrylamide gel (19:1 acrylamide:bisacrylamide) (Bio-Rad), 1X TBE ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by 1:2000 Tidy Blot Western Blot Detection Reagent-HRP (BioRad, Batch#160129) and the use of Immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Microbiology 2019Quote: ... both with horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (1:10,000; Bio-Rad). Anti-OmpA antibody54 was used at 1:20,000 and a custom anti-TssL antibody (Eurogentec ...
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bioRxiv - Microbiology 2019Quote: ... Amplification was performed in 10 μl reactions containing 1× iQ Supermix (BioRad, Mississauga, ON), 800 nM of each primer ...
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bioRxiv - Immunology 2020Quote: ... A 1:2000 dilution of isotype specific goat anti-mouse horseradish peroxidase conjugate (Biorad) in PBS supplemented with 2 % (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was generated using 1 μg of template RNA (iScript RT Supermix, BioRad, 1708841). cDNA was diluted 1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and a rabbit anti-vasoactive intestinal peptide (1:500, catalog: 9535-0204, Bio-Rad). The secondary antibodies used in a study were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... and primer sets (Supplementary Table 1) in a CFX96 Real-Time System (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... incubated with a secondary antibody (1:3000 HRP–linked Anti-Rabbit antibody Bio-Rad) and protein bands were visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermofisher Scientific #34580 ...
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bioRxiv - Bioengineering 2020Quote: 1 mm-thick micropatterned separation gels were stored in 1X tris-glycine (Bio-Rad) and buffer-exchanged to lower conductivity sucrose-dextrose dielectrophoresis buffer (DEP buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Nonspecific binding was blocked for 1 hr at RT with 5% BLOTTO (Bio-Rad) in Tris-buffered saline with 0.1% Tween (TBST) ...
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bioRxiv - Physiology 2020Quote: ... mouse monoclonal anti-Apolipoprotein A1 (Apo-A1) (0650-0050, Bio-Rad Laboratories, 1:200). Membranes were washed 3 times with TBST and incubated with anti-mouse IgG secondary antibody (A16017 ...
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bioRxiv - Cell Biology 2021Quote: ... and StarBright® B700 labeled goat anti-rabbit IgG (Bio-Rad, 12004161, 1:5000).
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bioRxiv - Microbiology 2020Quote: ... The bead/lysate mixture was poured onto a 1 cm separation column (Bio-Rad), the resin was allowed to pack and was washed with Wash Buffer (25 mM Tris-HCl [pH 8.0] ...