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Citations for Bio-Rad :
551 - 600 of 3006 citations for Interstitial collagenase MMP 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Ssp6-HA was detected using anti-HA primary antibody (MRC PPU Reagents, University of Dundee, 1:6,000) and HRP-conjugated anti-mouse secondary (1:10,000; Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... HRP (horseradish peroxidase)-conjugated goat anti-mouse (#176516, 1:10,000 for WB) and anti-rabbit IgG antibodies (#176515, 1:10,000 for WB) were from Bio-Rad. Alexa Fluor conjugated goat anti-mouse (1:500 for IF) ...
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bioRxiv - Microbiology 2020Quote: ... membranes were blocked overnight in PBST supplemented with 5% non-fat dry milk and incubated with antiserum (1:5,000) or vaginal washes (1:500) from test or control mice followed by goat anti-mouse IgG (BioRad) or IgA (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1 h at room temperature with peroxidase-conjugated anti-mouse (1:3000; BioRad, Hercules, CA) or anti-rabbit (1:3000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected with anti-rabbit (1:3,000) and anti-mouse (1:20,000) secondary antibodies coupled to horseradish peroxidase (Bio-Rad), diluted in 3% BSA-PBST ...
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bioRxiv - Biochemistry 2022Quote: ... the responses obtained from the AmQNS-small molecular interactions at different concentrations were fitted using the Langmuir 1:1 biomolecular interaction model using the ProteOn Manager software version 3.1.0.6 (Bio-Rad, USA). Equilibrium dissociation constants (KD ...
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bioRxiv - Microbiology 2020Quote: One ug of each respective protein was mixed at a 1:1 ratio with 2X Laemmli buffer (Bio-Rad) which was supplemented with 2% β-mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... or by anti-Smc3 K112/K113 Acetylation (1:1,000, gift from Dr. Katsuhiko Shirahige) in combination with goat anti-mouse HRP (1:10,000) (Bio-Rad) and ECL prime (GE Healthcare ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged to pellet tissue debris and equal volumes mixed 1:1 with 2x Laemmli sample buffer (BioRad). Samples were boiled ...
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bioRxiv - Biochemistry 2021Quote: ... was performed with 1:1,000 or 1:100,000 diluted PCR1 product using SsoAdvanced Universal SYBR green supermix (Bio-Rad). To quantify the Pten 5-6k isoform ...
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bioRxiv - Genetics 2019Quote: ... at 4° C with 100 V for 1 hour in 1 x Tris/Glycine Buffer (Bio-Rad, Cat#1610734) and 20% methanol (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... GroEL was detected with anti-GroEL primary antibody (Pierce; 1:10,000) and anti-rabbit secondary antibody (Biorad; 1:20,000).
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Bioengineering 2023Quote: ... A 10 μL aliquot of each assembly reaction was mixed 1:1 with Native Sample Buffer (Bio-Rad Laboratories), loaded into precast 4-15% polyacrylamide gels (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2023Quote: PFA fixed brain samples were incubated in 1% hydrogel monomers (HMs) consisting of 1% w/v acrylamide (#1610140, BioRad), 0.05% w/v bisacrylamide (#1610142 ...
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bioRxiv - Genomics 2022Quote: Antibodies and their working concentrations: anti-mouse (1:5000; #7076, Cell Signaling Technology, or 1:20000, #STAR117D800GA, Bio-Rad), anti-rabbit (1:5000 ...
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bioRxiv - Biochemistry 2023Quote: ... The nitrocellulose membrane was exposed to a 1:1 v/v solution of Clarity Western ECL Substrate (Bio-rad) for 5 mins and chemiluminescence quantified and analysed using FluorChem E software (ProteinSimple).
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed three times with TBST and then incubated for 60 min at room temperature with horseradish peroxidase-conjugated secondary antibodies (1:3,000 dilution, ref: 172-1011 and 1:5,000 dilution, ref: 170-6515, Bio-Rad) in blocking buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded onto polyacrylamide native gels (5% polyacrylamide 29:1 [BioRad], 1× TBE, 0.3% APS, and 0.06% Temed) and protein/DNA complexes were resolved by electrophoresis at 60 V for 2 h at 24 °C in 1X TBE ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified mouse α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Microbiology 2019Quote: Approximately 1 × 105 bacterial cells were suspended in PBS containing 1% (w/v) PBS-BSA and incubated with fH (Biorad) at a final concentration of 5 μg/mL for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were washed three times for 5 min and incubated with either a 1:5000 (Mouse)/1:10000 (Rabbit) dilution of horseradish peroxidase-conjugated secondary antibodies or with fluorophore-conjugated 1:5000 dilution of Starbright700 (Biorad) for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified goat α-rabbit or goat α-mouse IgG conjugated to alkaline phosphatase ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated secondary antibodies were diluted 1:10000 and 1:2000 for polyclonal Goat-α-Mouse (Bio-Rad, 170-6516) and Goat-α-Rabbit HRP (Dako ...
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bioRxiv - Genomics 2021Quote: ... 1 mM EDTA at pH 8.3) and by pulse field gel electrophoresis in 1% Certified™ Megabase Agarose (Bio-Rad) in 1X TAE buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were next washed and incubated for 1 hour at room temperature with peroxidase conjugated anti-rabbit (1:5000, BioRad), or anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... 18μg of sample was loaded per well diluted 1:1 with freshly prepared 2X Laemmli Sample buffer (BioRad Hercules, CA) + β-Mercaptoethanol (1:20 ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were then treated with HRP-conjugated goat anti-moue secondary antibody (1:10000) diluted in blocking solution for 1 h and developed with enhanced chemiluminescent (ECL) substrate (Cat#1705060, Biorad).
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bioRxiv - Biochemistry 2020Quote: ... the membranes were incubated with goat anti-rabbit antibody conjugated to HRP (1:500 or 1:1000 against MEK or ERK/GST, respectively) (BioRad) for one hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... blots were incubated for 1-2 h at RT with an anti-rabbit HRP-conjugated secondary antibody (diluted 1 : 3,000, Bio-Rad) in TBST with 5% milk ...
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bioRxiv - Pathology 2019Quote: ... After incubation of the blots with HRP-conjugated secondary antibodies (1:3000 for goat anti-rabbit or 1:10,000 for goat anti-mouse, Bio-Rad), specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagent Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell and tissue lysates were diluted at a 1 to 1 ratio of lysate to loading buffer and loaded onto Mini-Protean TGX precast gels (BioRad), electrophoresed ...
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bioRxiv - Physiology 2020Quote: ... antibody at 4°C overnight and in goat anti-rabbit IgG horseradish peroxidase conjugated secondary (1:5000) antibody for 1 hour before detection using ECL detection (BioRad) and ChemiDoc Imaging (BioRad) ...
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bioRxiv - Genetics 2020Quote: ... nduo-5 and ctb-1 with act-1 as control by iQ™ SYBR® Green Supermix (BIO-RAD, #1708880).
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bioRxiv - Microbiology 2020Quote: ... Maltose-1-phosphate was separated from orthophosphate by anion exchange chromatography using Dowex 1 × 8 100-200 mesh (Bio-Rad, 28 x 1.6 cm ...
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated in a 5 ml 1:1 A:B solution of Clarity Western ECL substrate (BioRad cat. #170-5060) for 5 minutes at room temperature with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Corresponding mouse or rabbit horseradish peroxidase-conjugated secondary antibodies (diluted 1:10,000 in 1x TBST) were added for 1 hr at room temperature (Bio-Rad). Chemiluminescence was detected by adding Clarity Western ECL Substrate (1:1 of luminol and peroxide solutions ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for immunoblotting: Talin-1 (1:5000 dilution, Mouse monoclonal, Clone 97H6; Bio-Rad, Cat# MCA4770), YAP (1:1000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 hour at RT with 1:10,000 goat anti-rabbit horseradish peroxidase (HRP) (Bio-Rad, Cat# STAR208P). The membrane was washed 3 times for 5 minutes with TBST ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and antimouse secondary antibody (Biorad; 1:10,000). GroEL was used as cytoplasmic control and detected as above ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies (Anti-Rabbit or Anti-Mouse, CST, 1:5000 and 1:2000) and revealed using Clarity Western ECL (BioRad). Image J was used to quantify the intensity of western blot bands ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were washed in 0.1% TBS-Tween and incubated with the secondary antibody (1:10000) for 1 h at room temperature (RT) and revealed by chemiluminescence (Clarity ECL western blotting substrates, BioRad) using ImageQuant LAS 500 chemiluminescence CCD camera (GE Healthcare Bioscience AB) ...
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bioRxiv - Biochemistry 2023Quote: ... expression media samples were diluted with 2X reducing SDS sample buffer at 1:1 ratio and the mixture was loaded on a 10-well 4–20% SDS-PAGE gradient gel (BioRad). Image J software was used to quantify the signal compared to the negative control (mock transfected culture) ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies and dilutions were used for 1 hour at room temperature: goat anti-rabbit StarBright Blue 700 (1:2,500; Bio-Rad), goat anti-rabbit IRDye 800 CW (1:5,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... sheep polyclonal to TGN46 (Biorad #AHP500G, 1:1000), rat monoclonal to myc-tag (Chromotek #9e1 ...
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bioRxiv - Developmental Biology 2021Quote: ... V5 (Bio-Rad: DyLight®550, 1:800), rat anti-DN-cadherin (DSHB ...
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bioRxiv - Cell Biology 2020Quote: ... packed with AGMP-1 resin (Bio-Rad Laboratories). Samples (100 μl ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 1% β-mercaptoethanol (Bio-Rad, 1610710) and RNA was isolated using the RNeasy Mini Kit (Qiagen 74104 ...
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bioRxiv - Microbiology 2020Quote: ... and permeabilized with 1% Triton X-100 (BioRad) in PBS for 10 min ...
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bioRxiv - Systems Biology 2022Quote: ... rat anti-F4/80 (BioRad, MCA497R, 1:100) or rabbit anti-CYP2E1 (Sigma ...