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Citations for Bio-Rad :
551 - 600 of 642 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
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bioRxiv - Biophysics 2024Quote: ... containing 20 mM MgCl2 and exposed to a thermal annealing ramp (65 °C, 15’; 58-53 °C, 1 h/°C; stored at 20 °C) in a Tetrad2 (Bio-Rad) thermocycler ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then separated by 20% Urea-PAGE gel at 250 V for 1 h and the fluorescent signal was captured by the ChemiDoc System (Bio-Rad). For protein detection ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with primary antibodies at 4°C overnight and subsequently incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and all images were acquired using a ChemiDoc XRS+ system (Bio-Rad). Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... at room temperature [goat anti-mouse 170-6516 or goat anti-rabbit 170-6515 IgG (H+L) HRP conjugate (Bio-Rad)] were all used at 1:2000 in 3 % BSA in 0.1 % Tween 20 (PBS/T) ...
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bioRxiv - Biochemistry 2022Quote: Proteins were reduced with 1 mM TCEP for 1 h then passed through a 10DG desalting column (Bio-Rad, Hercules, CA) to remove the TCEP ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... Infiltrated leaves were infiltrated with 100 µM D-luciferin and LUC activity was quantified after 48 h using a low-light CCD imaging apparatus (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Bioengineering 2022Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Cell Biology 2022Quote: ... The OD595 for each strain was recorded at the beginning and end of an 11-h incubation period at 30°C using an iMark Microplate Absorbance Reader (Bio-Rad). A detailed outline of the SGA procedure is included in Table S4.
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Cell Biology 2023Quote: ... plates were incubated at 25 °C for 30 h before imaging on a scanner and transilluminator (Bio-Rad Gel Doc XR+).
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad). The membranes were then washed three times (15 min each at room temperature) ...
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bioRxiv - Molecular Biology 2023Quote: ... embryos surviving for 24 h post- injection were incubated in 100 µl of 5 % Chelex® 100 Molecular Biology Grade Resin (BioRad) and 50 µl of Proteinase K Solution (20 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 60 μL of secondary goat antibody anti-rabbit IgG coupled with horseradish peroxidase (Bio-rad Goat Anti-Rabbit IgG (H+L) HRP conjugate 170-6515 ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated on 10 % polyacrylamide gels and blotted onto a nitrocellulose membrane at 100 V for 1 h using the Mini Trans-Blot system (Bio-Rad). Membranes were blocked with 5 % milk powder in PTw (1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Oligomer formation was induced by incubating the peptide solution for 24 h at 4 °C and confirmed by polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining.
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed five times with PBS-T and incubated with HRP-coupled secondary antibody for 1 h at room temperature and imaged with the ChemiDoc Imaging System from Bio-Rad. Relative protein levels were quantified with Image Lab (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated by SDS-PAGE on a 10% gel at 80 V for 3.5 h and transferred to a PVDF membrane via Trans-Blot® Turbo™ system (BioRad), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and incubated for 2 h with the appropriate HRP-conjugated secondary mouse antibody (1/1500, BioRad Laboratories) or secondary goat anti□rabbit IgG HRP (1/5,000 Cusabio Cedarlane ...
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bioRxiv - Neuroscience 2024Quote: ... at 1-4 °C and 100 volts for 1 h using Criterion™ blotter with wire electrodes (BioRad, Cat#1704071, USA). Blots were rinsed with phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by electrophoresis at 120 Volts for 1.5 h and then electroeluted onto a PVDF membrane (BIO-RAD, #10026933) at 25 Volts ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples from the co-IP and were separated on 8% SDS-PAGE gels for 1 h at 120 V and transferred overnight at 20 V on to PVDF membranes (Bio-Rad). Blots were probed with rabbit anti-NMIIB/Myh10 (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were separated on 4-20% Tris-glycine gels using 150 V over 1.5 h in a Criterion cell (Bio-Rad, USA). Proteins were transferred onto 0.45 um nitrocellulose membranes at 300 mA for 1.5 hr in a Criterion blotter (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The membranes were then washed with TBST again 3 times for 15 min before adding Goat Anti-Mouse IgG (H + L)-HRP antibody (1706516, Bio-Rad) 1:10000 in blocking buffer ...
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bioRxiv - Bioengineering 2024Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
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bioRxiv - Cell Biology 2024Quote: ... transferred to a nitrocellulose membrane (Cytiva) for 2 h (at 30V) and detected using infrared light on a Chemidoc MP (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in TBS-T prior to incubation with goat anti-rabbit IgG (H+L)-Horseradish Peroxidase Conjugate (#1706515, Bio-Rad) or donkey anti-Goat IgG (H+L)-Horseradish Peroxidase Conjugate (Invitrogen #A15999 ...
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bioRxiv - Neuroscience 2021Quote: ... were mixed with filtered monomeric α-syn WT (30 μM) and ThT (10 μM) and were incubated at 55°C for 5 h in a PCR machine (Bio-rad, C1000) for amplification.[3] Fractions comprised of ≥ 76% of its original volume were mixed with α-syn and ThT to reach a final volume of 20 μL per reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed three times for 10 minutes with 1x PBS and incubated with Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad, #172-1011). Chemiluminescence was induced by incubation with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Physiology 2020Quote: ... Blood amino acids were extracted from 500 uL of supernatant by cation exchange chromatography (Dowex AG 50W-8X, 100–200 mesh H+ form; Bio-Rad Laboratories). Phenylalanine enrichments were determined by gas chromatography–mass spectrometry (GC-MS ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated for 1 h at RT with goat anti-Lipid A LPS antibodies (diluted 1:1,000 in blocking buffer; Bio-Rad; Cat # OBT1844). Blots were washed three times with TBST (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2021Quote: ... the plates were incubated at 37°C for 24 h in ambient air and growth was assessed at OD595 using microplate reader (iMark, Bio-Rad, Japan). The lowest concentration that completely prevented bacterial growth was categorized as MIC.
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Microbiology 2020Quote: ... The development of the colonies of the fluorescently labeled Trichoderma strains was monitored by making regular (every 24 h) images in a Bio-Rad ChemiDoc MP system (Bio-Rad, USA) equipped with multiplex fluorescent channels ...
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bioRxiv - Microbiology 2020Quote: ... and purified from remaining salts using Dowex 50 W X 8 50-100 mesh (Bio-Rad, 10 x 1 cm, H+ form) equilibrated with water ...
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bioRxiv - Neuroscience 2022Quote: ... The gel was subject to 100 V for 3.5 h and the gel underwent semi- dry transfer by a Trans-Blot® Turbo™ Transfer System (Bio-Rad) onto a 0.2 µm polyvinylidene fluoride membrane at 1.3 A for 11 min ...
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bioRxiv - Cell Biology 2023Quote: ... wre incubated for 1 h at RT and membranes were revealed by using ECL reagents with the ChemiDoc imager (Bio-Rad Laboratories).
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bioRxiv - Physiology 2023Quote: ... The blots were washed four times with 0.1% tween TBS before being incubated for 1 h at room temperature with goat Anti-rabbit IgG HRP conjugated secondary antibody (Bio-Rad, USA) in 0.1% tween TBS buffer containing 3% BSA (Bovogen ...
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Synthetic Biology 2022Quote: ... The solid cultures were grown at 30ºC without shaking for 48 h and pictures of cell plates were taken using the colorimetric mode of Chemidoc Touch Imaging Systems (Bio-Rad, USA). All halo assays were conducted in three biological replicates.