Labshake search
Citations for Bio-Rad :
551 - 600 of 608 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Wells were washed five times with PBS+0.05% Tween 20 and blocked with PBS+5% dry milk solution (Bio-Rad, 1706404) for 1 hour at room temperature ...
-
bioRxiv - Genetics 2024Quote: ... Approximately 43 µl of the droplet solution was transferred to a ddPCR 96-well plate and sealed in a PX1 PCR plate sealer (Bio-Rad) followed by PCR at 95°C for 10 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... to make 8 mL of 9% resolving Phos-tag gel solution (enough for one 1.5 mm Bio-Rad mini gel cast), the following reagents were mixed together evenly ...
-
bioRxiv - Neuroscience 2024Quote: ... and then rinsed with a solution of 40% ethanol/10% acetic acid before being air dried overnight and imaged on Chemidoc (Bio-Rad).
-
bioRxiv - Biophysics 2024Quote: ... The gels were run at 130 V until the protein standard lanes were resolved to satisfaction and then stained with Coomassie Brilliant Blue R-250 staining solution from Bio-Rad. Gels were de-stained with the Coomassie Brilliant Blue R-250 de-staining solution.
-
bioRxiv - Neuroscience 2024Quote: ... the membranes were washed three times with TBST for 10 minutes each and incubated with secondary antibody solutions (HRP-conjugated secondary antibodies: Bio-RAD #1705045 or #1705047 ...
-
bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
-
Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... we separated beads from solution and transferred 44 μL of the supernatant (containing the DNA) into a new 96-well PCR plate (Bio-Rad HSP9631) for library preparation ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™ ...
-
bioRxiv - Physiology 2020Quote: ... tissue biopsies were transferred into the assay medium for a 4h incubation (pre-incubation medium supplemented with 4 mg/ml glucose and 4.3% Alamar Blue solution (Bio-rad, Hercules, CA)) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and MgCl2 [12.5 mM]) and annealed by cooling the solution from 95 to 12 °C over 30 min in a thermal cycler (Bio-Rad, T100). Detailed descriptions for the assembly of individual gate complexes are described in the supplementary information ...
-
bioRxiv - Biophysics 2020Quote: ... 50 µl of a 10− 6 M biotinylated and 10−7 M dye-coupled oligonucleotide solution were co-incubated to anneal the oligonucleotides at 80 °C using a Thermocycler (BioRad, C1000 ThermoCycler), followed by sample cooling to 4 °C with -1 °C/min ...
-
bioRxiv - Neuroscience 2022Quote: ... then placed in 4% normal goat serum in 0.01M PBS blocking solution for 60 minutes with agitation followed by incubation with rat anti-CD68 (1:500; BioRad, cat no. MCA1957GA) and rabbit anti-MBP (1:1000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... for 1 hour at RT and after incubation the blots were developed in an ECL detection solution (Clarity Max ECL substrate, Bio-Rad Laboratories) and signal was detected using ChemiDoc (BioRad Laboratories).
-
bioRxiv - Neuroscience 2020Quote: ... The visualized signal bands were detected using an ECL solution (Amersham, Buckinghamshire, UK) through a ChemiDoc XRS System (Bio-Rad, Hercules, USA). The p/t form volumes were calculated and quantified using ImageJ software ...
-
bioRxiv - Neuroscience 2021Quote: ... The solution was then drawn into the previously prepared Tezfel tubing and placed into a tubing prep station (Bio-Rad, Hercules, CA) for rotation until even distribution of the tungsten was achieved ...
-
bioRxiv - Bioengineering 2021Quote: ... We developed western blot membranes with enhanced chemiluminescence (ECL) solution and images were captured with a luminescent image analyzer ChemiDoc (Bio-Rad, USA) or Amersham Imagequant 800 (Cytiva ...
-
bioRxiv - Bioengineering 2021Quote: ... We developed western blot membranes with enhanced chemiluminescence (ECL) solution and images were captured with a luminescent image analyzer ChemiDoc (Bio-Rad, USA).
-
bioRxiv - Neuroscience 2023Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS solution with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
-
bioRxiv - Neuroscience 2024Quote: ... Mice were anesthetized and transcardially perfused with ice-cold PBS/heparin (50 u/ml) solution (Bio-Rad, Cat #1610780; Sigma, Cat# H3393), followed by 10% formalin (Polyscience ...
-
bioRxiv - Microbiology 2024Quote: ... sodium cations were exchanged with pyridinium ions by dissolving 20 mg of fucan in water and passing the solution through an AG® 50W cation exchange resin (Bio-Rad), pre-equilibrated with pyridine (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: ... the protein blots on the PVDF were detected using ECL luminous solution (EpiZyme, Shanghai, China) in ChemiDoc MP electrophoretic imaging system (Bio-Rad, USA), and the protein levels were quantified using Fiji software ...
-
bioRxiv - Developmental Biology 2023Quote: ... Transfer buffer was prepared as a 1X final solution by mixing 7:2:1 of ddH2O: methanol: 10X Tris/Glycine Transfer Buffer (Bio-Rad #1610734). Following transfer ...
-
bioRxiv - Cell Biology 2023Quote: ... The beads were then washed twice with PBS and twice with TBS and eluted with a 250 mM imidazole solution in TBS using Poly-Prep Chromatography Columns (Bio-Rad, 7311550). The eluates were loaded onto a Superose 6 Increase 10/300 GL column on an AKTA purifier (GE Healthcare ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: Equal amounts (10 μL) of the eluted solution from the streptavidin pulldown were loaded in a 4-20% polyacrylamide gel (Bio-RAD 4561096) and separated with SDS-PAGE at 100 V for 1 hour ...
-
bioRxiv - Physiology 2023Quote: ... Images were obtained by developing with ECL solution and image acquisition with a Biorad ChemiDoc gel imager with ImageLab software (Biorad, Hercules, CA). Densitometry with automated background substraction was performed with ImageLab (Biorad ...
-
bioRxiv - Plant Biology 2023Quote: ... and 0.01% Silwet L-77 (Lehle Seeds Inc) solution followed by incubation in the dark for 10 min and imaging using a ChemiDoc™ Touch System (Bio-Rad). Alternatively ...
-
bioRxiv - Bioengineering 2023Quote: ... at pH 8.0 in aqueous solutions were made and then subjected to thermal annealing in a thermal cycler (Bio-Rad, Hercules, CA) for self-assembly ...
-
bioRxiv - Zoology 2024Quote: Genomic DNA was extracted from the right hind leg of individual worker bee using a 5% (w/v) Chelex solution (Chelex®100; BIO-RAD), as followed by Walsh ...
-
bioRxiv - Cancer Biology 2024Quote: ... STOP solution was added to each well and absorbance was measured at 450 nm using iMark™ Microplate Absorbance Reader (Bio-Rad). Graphs display relative phospho-JNK levels.
-
bioRxiv - Microbiology 2021Quote: ... The bound dye in the wells was eluted with 33% acetic acid and the absorbance of the solution was read at 595nm in a microtitre plate reader (BioRad i-Mark, Japan). To assess the individual bacterial population during growth ...
-
bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
-
bioRxiv - Physiology 2021Quote: ... The solution with MSP1E3D1 was mixed at 4°C for 10 min before addition of 130 mg of Biobeads SM2 (Bio-Rad, Hercules, CA). Biobeads (washed into methanol ...
-
bioRxiv - Developmental Biology 2020Quote: ... protein samples were boiled with β-mercaptol and ran in SDS-PAGE gels casted from 30% Acrylamide/Bis solution 29:1 (Bio-rad, 1610156) using the Mini-PROTEAN® system (Bio-rad) ...
-
bioRxiv - Molecular Biology 2022Quote: ... the protein bands were detected by LumiQ HRP Substrate solution (Share-bio, SSA003) and imaged with ChemiDoc™MP imaging system (Bio-Rad, USA)
-
bioRxiv - Bioengineering 2024Quote: ... The absorbance of each solution was measured at a wavelength of 450 nm using a UV–vis microplate reader (Bio-Rad, Philadelphia, PA).
-
bioRxiv - Developmental Biology 2023Quote: ... The assay was performed in a 96-well plate in a final volume of 200 µL containing the sample and Bradford’s solution (BioRad, Marnes-la-Coquette, France). After a 5-min incubation at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... each lysate was diluted at a 1:1 ratio with a solution of 2× Laemmli sample buffer (#1610737EDU, Bio-Rad, Hercules, CA, USA), containing 5% (v/v ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the total lysates (20-30 µg/lane) were added with a solution to reduce the disulfide bridges (Reducing Agent 20X; Bio-Rad, Milan, Italy) and Laemmli Sample Buffer 4X (Bio-Rad ...
-
bioRxiv - Plant Biology 2024Quote: ... the membrane was washed twice with the 1x TBS-T buffer and then incubated with the Clarity Western ECL Substrate solution (Bio-Rad, California, USA). Signal detection of the membranes was performed by the Chemi-Doc Imaging system (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: ... and the protein bands were visualized with an enhanced chemiluminescence (ECL) solution (Clarity Western ECL Substrate, 1705062 Bio-Rad Labs, Des Plaines, IL). Quantitative analysis of the bands was performed with ImageJ software (version 1.49 ...
-
bioRxiv - Neuroscience 2024Quote: ... The transfer was carried out to 0.45 μm nitrocellulose membranes for 20 min per membrane at constant 25 mA in a semi-dry transfer chamber Trans-Blot® Turbo™ Transfer Solution from Bio-Rad (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2024Quote: ... Mice were anesthetized and transcardially perfused with an ice-cold PBS/heparin (50 u/ml) solution (Bio-Rad, Cat #1610780; Sigma, Cat# H3393), followed by 10% formalin (Polyscience ...
-
bioRxiv - Developmental Biology 2024Quote: ... A chemiluminescent signal was obtained from the blots using (ECL Prime Luminor Enhancer Solution, Cytiva, Canada) and the blot was developed using a ChemiDoc System (Bio-Rad Laboratories, Canada). Densitometries were calculated using ImageJ (Schneider et al. ...
-
bioRxiv - Bioengineering 2024Quote: ... Membranes were washed 3 more times before imaging with a 1:1 (v/v) ratio of Clarity Western enhanced chemiluminescence and peroxide solutions (Bio-Rad, Cat # 1705061). Primary and secondary antibodies used include ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μl of the resulting solution was then applied to either 4-12% Criterion™ XT Bis-Tris Protein Gels (BioRad, Hercules, CA, USA) or NuPAGE™ 4 – 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: ... The re-suspended DNA droplet solution was then denatured at 95 °C for at least 5 min on a C1000 Touch™ Thermal Cycler from Bio-Rad (CA, USA). The aqueous solution consisted of 25% v/v SUV solution (containing 8mM lipids as prepared above ...
-
bioRxiv - Genetics 2020Quote: ... tissue samples were incubated with 20 μl of base solution (25 mM KOH, 0.2 mM EDTA) at 95 °C for 30 min in a thermal cycler (BIORAD C100, BIORAD, Hercules, CA, USA), then 20 μl of neutralization buffer (40 mM TrisHCl ...
-
bioRxiv - Developmental Biology 2023Quote: ... The membranes were washed with TBST three times and then incubated with a secondary antibody for 1 h before using Luminol/enhancer solution and ClarityTM Western ECL Substrate (Bio-Rad Laboratories, Inc. US). Western blot images were scanned by using Gel Doc XR system (Bio-Rad Laboratories ...
-
bioRxiv - Biophysics 2024Quote: ... heated to 37°C for 10 min and resolved by SDS-PAGE in 10% (for Cx43 detection) and 12% (for AQP4 and GFAP detection) gels prepared with Mini-PROTEAN TGX Stain-Free Precast polyacrylamide solutions (Bio-Rad, Hercules, California, USA). gels were then activated for 5 minutes on a Chemidoc imaging system (Bio-Rad ...