Labshake search
Citations for Bio-Rad :
551 - 600 of 2104 citations for 7 methyloct 3 en 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1000, BioRad). As secondary antibodies Horseradish Peroxidase (HRP ...
-
bioRxiv - Biochemistry 2020Quote: ... Plates were blocked with 3% nonfat dry milk (BioRad, P1379) in 1xPBS for 1 hour ...
-
bioRxiv - Plant Biology 2022Quote: ... on 3 technical replicates using PCR SYBR GreenSuperMix (1725261, BioRad) and primers listed in Table S1 ...
-
bioRxiv - Cell Biology 2023Quote: ... 3 was performed using an iCycler thermocycler (Bio-Rad laboratories) with a TaqMan RT-PCR Master Mix (Bio-Rad Laboratories ...
-
bioRxiv - Genomics 2023Quote: ... 5′-CCCCCCATCTGATCTGTTTCAC-3′) by qPCR using SsoFast EvaGreen Supermix (BioRad), according to the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
-
bioRxiv - Developmental Biology 2020Quote: ... 30 second at 72°C and a final extension of 7 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). Five μl of PCR product was visualized on a non-denaturing 1% agarose gel with a 100bp ladder ...
-
bioRxiv - Neuroscience 2021Quote: ... 40 min) and were subsequently transferred to nitrocellulose membranes for semi-dry immunoblotting (25 V, 1,3 A, 7 min, Bio-Rad®). Membranes were dried 5 min at 37°C and then incubated with 5% non-fat dried milk in Tris-buffered salineTween-20® (TBST containing 28 mM Tris ...
-
bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
-
bioRxiv - Microbiology 2021Quote: Two micrograms of each RNA sample was loaded on 10% polyacrylamide gels containing 7 M urea or loaded onto 10% Criterion TBE-urea precast gels (Bio-Rad) and electrophoresed at 85 V ...
-
bioRxiv - Pathology 2021Quote: ... Real-time PCR was performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo-Fisher) using the iTaq Universal SYBR green Supermix (Bio-Rad). The following primer sequences were used ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were transferred electrophoretically at a constant voltage of 25 V for 7 min using a Trans-Blot Turbo Transfer System (Bio-Rad). After transfer ...
-
bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
-
bioRxiv - Immunology 2021Quote: ... P2 was dialyzed against 1% glycine containing 2% ampholytes pH 3-10 (first run) or pH 5-7 (second run) and then loaded onto a liquid isoelectric focusing system (Rotofor, Bio-Rad). Individual fractions were harvested and their pH and absorbance at 280nm were measured ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 30 °C for 7 days and imaged daily using a gel documentation system (Gel Doc™ XR+, BIORAD). When needed ...
-
bioRxiv - Biochemistry 2020Quote: ... the buffer of the purified DNAJB6 was exchanged into 10 mM ammonium acetate pH 7 solution using Micro Biospin P6 centrifuge columns (Bio-Rad) and protein concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
-
Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... RNA was transferred to a nylon membrane (Amersham Hybond-XL) at 23 V for 7 minutes using a Trans-Blot Turbo Transfer System (Bio-Rad). Following transfer ...
-
bioRxiv - Cell Biology 2023Quote: Protein samples were resolved on 7-15% gradient polyacrylamide (SDS-PAGE) gels and transferred to nitrocellulose membranes via Trans Blot Turbo (Bio-Rad). Membranes were then blocked with 5% milk in Tris-buffered saline and 0.05% Tween 20 (TBST ...
-
bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
-
bioRxiv - Biochemistry 2022Quote: ... 1:10 000 dilution for N-terminal Parkin Ab) was incubated one hour at RT and protein visualized using ClarityTM Western ECL Substrate (Bio-Rad).
-
bioRxiv - Biochemistry 2020Quote: ... and the quantity of radiolabel included into the acid insoluble fraction was analysed using Quantity One software (Bio-Rad). The quantitative data were analysed using MS Excel 2010 and presented on histograms as the Mean ± SD.
-
bioRxiv - Microbiology 2021Quote: ... PCR reaction mixtures were as follows: 5.5 μl One-Step RT-ddPCR Advanced Kit for Probes Supermix (no dUTP’s) (Bio-Rad), 2.2 μl reverse transcriptase ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed at 150V for one hour through 7.5% or 4-20% mini-PROTEAN TGX gels (Bio-Rad) and blotted at 300 mA for one hour onto PVDF membranes ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... kit and BioRad chemiluminiscence machine scanner.The densitometry analysis of immunoblot images was performed using quantity One Software(Bio-Rad). Densitometry values were normalzed to the loading control(Gapdh or Actin ...
-
bioRxiv - Cell Biology 2021Quote: ... Real-time PCR reactions were set up using the iTaq Universal Sybr Green One-Step RT-PCR kit (Biorad) according to manufacturer’s directions ...
-
bioRxiv - Microbiology 2021Quote: ... Viral RNA was then quantified by qRT-PCR using the iTaq™ Universal Probes One-Step Kit (BIO-RAD) according to manufacturer’s protocol using a forward primer (5’-CCGCTGCCCAACACAAG-3’) ...
-
bioRxiv - Microbiology 2021Quote: ... and amplicons were generated via qRT-PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD) according to manufacture protocol ...
-
bioRxiv - Pathology 2022Quote: ... The expression levels of target proteins were quantified with Quantity One 1-D Analysis Software (Bio-Rad, Hercules, CA) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
-
bioRxiv - Cancer Biology 2022Quote: ... an iScript reverse transcription kit was used to convert one microgram of RNA to cDNA (Bio-Rad, Hercules, CA). No reverse transcriptase controls were included ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein-associated fluorescence intensities (arbitrary units, AU) were analyzed using Quantity One image analysis software (BioRad, Hercules, CA, USA). Gels were stained with Colloidal Coomassie Brilliant Blue G250 and scanned.
-
bioRxiv - Microbiology 2020Quote: One ug of each respective protein was mixed at a 1:1 ratio with 2X Laemmli buffer (Bio-Rad) which was supplemented with 2% β-mercaptoethanol (Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: Pictures were obtained with Bio-Rad Quantity One software implemented in a Bio-Rad GelDoc Imaging system (Bio-Rad Laboratories ...
-
bioRxiv - Immunology 2019Quote: ... The extracted RNA was amplified by iTaq universal SYBR Green one-step PCR kit (Bio-Rad, Hercules, CA, USA) and 10μM primers (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2019Quote: ... One aliquot from each time point was analyzed by SDS/PAGE using a Criterion 16.5% TRIS/TRICINE gel (BioRad) along with [14C]fIX -18/+41 standards ...
-
bioRxiv - Genetics 2020Quote: ... digital droplet PCR (ddPCR) was performed using the One-step RT-ddPCR advanced kit for probes (Bio-Rad, #1864022). The final 20 μL reaction mix contained ...
-
bioRxiv - Pathology 2021Quote: ... One gel was used for Western blotting and the other was stained with SYPRO Ruby gel stain (Bio-Rad) for visualizing total protein.
-
bioRxiv - Cancer Biology 2021Quote: ... One microgram of extracted RNA was reverse transcribed into cDNA using iScript cDNA Synthesis Kit (BIO-RAD, Cat #1708890). Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward ...
-
bioRxiv - Plant Biology 2022Quote: One microgram of RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative real-time PCR analysis was performed on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Neuroscience 2022Quote: ... Purified RNA was analyzed by TaqMan qRT-PCR one-step RT-PCR kit for probes (Bio-Rad laboratories, Italy) with a TaqMan RT-PCR instrument (CFX384 real time system ...
-
bioRxiv - Microbiology 2022Quote: ... Blots were photographed and revealed taking one picture every second for 10 seconds using ChemiDoc Imaging System (Bio-Rad). Blots were then annotated ...
-
bioRxiv - Microbiology 2023Quote: ... using manufacturers protocol and imaged on a ChemiDoc XRS Imaging System with Quantity One imaging software (Bio-Rad Laboratories).
-
bioRxiv - Plant Biology 2024Quote: ... The gels were visualised using a UV trans-illuminator Gel Doc 2000 and Quantity One 1-D analysis v4.6.5 software (Bio-Rad).
-
bioRxiv - Microbiology 2024Quote: ... Viral RNA titers and infections were assessed via qRT-PCR using an iTaq Universal Probes One-Step Kit (BioRad) in a 20 µl sample volume ...
-
bioRxiv - Microbiology 2024Quote: ... using 11 μL undiluted RNA and the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Cat# 1864021). Droplets were generated ...
-
bioRxiv - Immunology 2020Quote: ... the reaction mixture was passed through 2 Bio-Gel P-2 mini columns (1504114, Bio-rad) by centrifugation (3000 rpm ...
-
bioRxiv - Developmental Biology 2020Quote: ... complemented with 2-mercaptoethanol (Bio-rad) and heated at 95 °C for 5 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with 2-mercaptoethanol (Bio-Rad).
-
bioRxiv - Biophysics 2019Quote: ... containing 5% 2-mercaptoethanol (Bio-Rad), electrophoresed by SDS-PAGE and blotted onto nitrocellulose membrane ...
-
bioRxiv - Biochemistry 2020Quote: ... Bio-Beads SM-2 (Bio-rad) were supplemented into the mixture to remove the detergent ...