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Citations for Bio-Rad :
551 - 600 of 675 citations for 7 8 Dihydro 6 5H quinolinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Serum samples collected from mice were tested in duplicate in a multiplex immunoassay using magnetic beads (Mouse Th1-Th2 8-plex Bio-Rad, Hercules, CA) using the Luminex MAGPIX® system) ...
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bioRxiv - Biochemistry 2023Quote: ... plates were stained with primuline (0.05% in acetone/water, 8/2; v/v) and photographed under long-wave UV light (Biorad ChemiDoc XRS Imaging System).
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bioRxiv - Microbiology 2023Quote: ... for the selected clinical isolates using the indicated concentrations of antibiotics via an alamar blue reduction assay completed as described but without shaking.8 After 3 days of incubation with the alamar blue reagent (BioRad, Hercules, CA, USA), the MIC was identified as the lowest concentration of drug that inhibited the reagent color change as determined visually.
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bioRxiv - Cancer Biology 2024Quote: ... Serum hormones were quantified on a Luminex MAGPIX platform using the Bio-Plex Pro Mouse Diabetes 8-Plex and Adiponectin Assays (Bio-Rad, Hercules, CA) and the IGF-1 mouse Luminex Discovery Assay (R&D Systems ...
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bioRxiv - Biophysics 2021Quote: ... The uncoupled BG-GLA-NHS was removed by filtration on Micro Bio-Spin™ P-6 Gel Columns (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... approximately 250.000 cells were plated in a 6-well dish and transfected with a final concentration of 22.5 nM siRNA using siLentFect Lipid Reagent (Bio-Rad) accordingly to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... MtCoaBC and MsCoaBC were exchanged into NH4OAc (500 mM, pH 7.0) solution using Micro Bio-Spin 6 chromatography columns (Bio-Rad). A sample volume of 2.5 μL was injected into a borosilicate emitter (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... YpdA and YpdAC14A proteins were pre-reduced with 10 mM DTT followed by DTT removal with Micro Biospin 6 columns (Biorad). For the biochemical activity assays of the specific BSSB reductase activity ...
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bioRxiv - Biophysics 2019Quote: ... We then removed excess dye by four subsequent spins of the solution over Micro Bio-Spin 6 Columns (Bio-Rad) at 700 g for 2 min ...
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bioRxiv - Molecular Biology 2019Quote: ... EtOH precipitation was performed and DNA was additionally cleaned up on Micro Bio-Spin 6 chromatography columns in SSC (BioRad) as purity was essential for oxidation of hmC ...
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bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were boiled at 95 °C for 10 mins with 6×SDS sample buffer before loading onto 4–20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Biochemistry 2019Quote: ... The spin-labeled protein was further concentrated and free label was removed using Micro BioSpin 6 Chromatography Columns (Bio-rad) pre-equilibrated with buffer C.
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bioRxiv - Plant Biology 2020Quote: ... Captured elution fraction from the Strep-Tactin-affinity purification were loaded onto a anion exchange column (UNO Q-6, Biorad) with a flowrate of 4 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... pH 5.3 (murine P-domains) or pH 7.3 (GII.4 Saga P-domain) at 4 °C via Micro Bio-Spin 6 columns (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and CyaY were buffer exchanged into 200 mM ammonium acetate (pH = 7.5) using Micro Bio-Spin 6 Columns (Bio-Rad). Instrument parameters were tuned to maximize transmission but simultaneously preserve the native-like state of proteins as determined by ion mobility ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6) were quantified using a real-time PCR detection system and SsoAdvanced Universal SYBR Green Supermix kit (BIO-RAD). In brief ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
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bioRxiv - Biochemistry 2020Quote: ... the cleaved His6 tag was removed and the enzymes were buffer exchanged into 200 mM ammonium acetate (pH 7.5) using Micro Bio-Spin 6 columns (Bio-Rad). His6-affinity tagged HalA2 was likewise digested with thrombin to remove the His6 tag ...
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bioRxiv - Plant Biology 2021Quote: ... The IEF was performed on the 7cm length gel strips with immobilized pH gradients 3-10 and 3-6 (BioRad). After IEF ...
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bioRxiv - Biochemistry 2022Quote: ... the enzymes were buffer exchanged into their respective reaction buffers above using a Micro Bio-Spin 6 size exclusion spin column (Biorad) to remove glycerol and other ion suppressing constituents ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to PVDF membrane at 4°C for 14.5 h at 30 V using the mini trans-blot module in transblot buffer (10 mM MES buffer pH 6 and 10% (v/v) methanol) according to the supplier’s instructions (Bio-Rad). The membrane was removed from the cassette ...
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bioRxiv - Biophysics 2021Quote: ... and 2 times the critical micelle concentration (CMC) of various detergents (namely LDAO and C8E4) using a Biospin-6 column (BioRad) and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
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bioRxiv - Developmental Biology 2019Quote: ... Alcian Blue was extracted by 6 M guanidine hydrochloride solution and measured by optical density at 600 nm (O.D. 600) using spectrophotometer (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
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bioRxiv - Systems Biology 2021Quote: ... and 6 μl for slow growing mutants with lower OD600) on Stain-Free gels (4-20%, CAT # 4568096, Bio-Rad, Tris/Glycine SDS Buffer (CAT # 161-0732 ...
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bioRxiv - Microbiology 2021Quote: ... for 60 min at 37°C followed by purification on a Micro-Bio Spin P-6 Gel Column (Bio-Rad). 1 pmol of 5’-end-radiolabeled primer was used for each extension reaction ...
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bioRxiv - Neuroscience 2019Quote: ... Serum was isolated and IL-6 levels were measured using a Bio-Rad Luminex system (Bio-Rad, Laboratories, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: APRT2-Ntag and APRT2-Ctag were buffer exchanged into a volatile buffer (200 mM ammonium acetate, pH 7.4) using a Micro Bio-Spin 6 device (Bio-Rad). Samples were loaded into pulled glass capillaries prepared in house ...
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bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...
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bioRxiv - Biochemistry 2023Quote: ... CsgA and CsgAslowgo samples were buffer-exchanged into MS-compatible buffer (150 mM Ethylene diamine diacetate pH 6.3, 10mM TMAO) using Micro Biospin 6 desalting columns (Bio-Rad) to a final protein concentration of 3-5 μM ...
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bioRxiv - Plant Biology 2023Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE (pH 8.0) buffer and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Biochemistry 2023Quote: ... The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad). Molecular weight of standard was as follows ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Molecular Biology 2024Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE buffer (pH 8.0) and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... while insulin levels were assayed using the quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of transcribed RNA were electroporated into 8×106 Vero cells using the Gene Pulser Xcell™ Electroporation Systems (Bio-rad, California, USA) under as described previously41 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Twenty microlitres of the mastermix + template DNA were pipetted into the very bottom of the central row of (8) wells of the Droplet Generator DG8™ Cartridge (Bio-Rad 186-4008). Sixty-five microlitres of droplet generation oil for probes (BioRad 186-3005 ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: Frozen muscle biopsies were homogenised in modified Laemmli sample buffer as described previously.37 Samples were run in Bio-Rad Criterion 3–8 % tris-acetate gradient gels (Bio-Rad Laboratories, CA, USA) at room temperature ...
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bioRxiv - Immunology 2022Quote: ... cell debris was removed by centrifugation and clear supernatants were diluted 10-fold and subjected to anion exchange chromatography on gravity fed columns using AG-1 9 8 resin (formate form, 100–200 mesh size, Bio-Rad Laboratories, Hercules, CA). Fractions containing inositol mono ...
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bioRxiv - Biochemistry 2020Quote: ... resolved on the 8% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 µm pore size) with the use of a Trans-Blot Turbo Transfer Starter System (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... cell viability was determined using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) by measuring the absorbance at 450 nm using an iMarkTM microplate absorbance reader (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Forty µg denatured protein per well was resolved by 8-12% SDS- PAGE homemade gels in parallel with multi-colored molecular weight marker (Bio-Rad Laboratories, Cat.No.1610395). Gels were run at 100V for 1-2 h and transferred overnight at 8- 12V ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated on an 8-12% SDS-polyacrylamide gel and blotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), The membrane was incubated overnight at 4°C with primary Abs ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... IB analysis was performed after 6-7.5% SDS-PAGE or 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA), with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2019Quote: ... The libraries were quantified and checked for amplifiable adapters using the Library Quantification DNA standards 1-6 (Kappa Biosystems, Wilmington, USA) with the SsoFast EvaGreen qPCR supermix (Bio-Rad) using 10 μL EvaGreen master mix ...
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bioRxiv - Bioengineering 2019Quote: Equal volumes of (GT)6-Cy5-SWCNT and FAM-tagged protein at 2X working concentration were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...