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Citations for Bio-Rad :
551 - 600 of 7889 citations for 1 4 Bis acetyloxy 3 dodecylsulfanyl 2 naphthyl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4°C and for 1 h at room temperature (RT) with secondary horseradish peroxidase conjugated antibodies (Bio-Rad, 1:3000). Membranes were then developed using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed again with 1x PBS-T (0.075 %) 3 times 10 minutes each and incubated with 1:1 ECL chemiluminescence solution (Clarity Western ECL, #170-5061, Bio-Rad Laboratories, Hercules, CA, USA). Signal was detected using an Amersham AI600 imager (Supplementary Table 5).
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Biochemistry 2022Quote: ... The αM and β2 subunits of recombinant Mac-1 integrin were resolved on 4-20% polyacrylamide (BioRad), gradient gels by SDS-PAGE and stained with Coomassie brilliant blue R250 ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:4 and analyzed using Bio-Plex ProTM Human Chemokine Panel 40-plex Assay (Bio-rad). Acquisitions and analyses were performed on a Bio-Plex 200 system with Manager 6.1 Software (Bio-rad) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated shaking for 1 hour at RT with 4% blocking solution (5% milk powder (BioRad) in TBS-T) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2 M 2-Mercaptoethanol (Bio-Rad), and boiled for 3 min ...
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A tyrosine kinase protein interaction map reveals targetable EGFR network oncogenesis in lung cancerbioRxiv - Cancer Biology 2020Quote: ... 4 μl of the eluate was analyzed by 4-20% SDS PAGE (Biorad) and silver staining ...
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bioRxiv - Plant Biology 2024Quote: ... 4× Laemmli buffer and 4-20% SDS-PAGE gels were purchased from BioRad.
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system). Membranes were blocked with 5% milk (standard protein detection ...
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
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bioRxiv - Zoology 2020Quote: ... Samples were incubated in a 1:1 (w/v) ratio of 2× sample buffer (0.2M DTT, BioRad Laemmli Sample Buffer #1610737) at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rat Ly-6B.2 alloantigen (1:100, MCA771GA, Bio-Rad Laboratories, Hercules, CA) for neutrophils ...
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bioRxiv - Neuroscience 2020Quote: ... TBS-T) before incubation with HRP-conjugated secondary antibody for 2 hours (Biorad, 1:1000). After 3 times washes with 5 min intervals with TBS-T ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... using 1-2 ng cDNA per sample complemented with iQ SYBR®Green Mastermix (BioRad) and specific forward and reverse primers at a concentration of 0.5 µM each ...
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bioRxiv - Bioengineering 2024Quote: ... 1-5 µg of target protein was resuspended in 2× Laemmli Sample Buffer (Bio-Rad) with adding 1:20 Beta-mercaptoethanol and 15 µL of the mixture was added onto Any kD™ Criterion™ TGX Stain-Free™ Protein gel (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein lysate (30 μg) with 2X Laemmli buffer (in 1:1 ratio) was loaded onto a 4%–20% gradient gel (Bio-Rad, Mississauga, ON, Canada). The gel was transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... 3–8% Tris-acetate gels (Life Technologies. Proteins were transferred to nitrocellulose membrane by semi-dry transfer (Trans-Blot Turbo transfer system; Bio-Rad) or ...
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bioRxiv - Plant Biology 2022Quote: ... Blots were washed with TBST buffer three times for 10 min each time and inserted between acetate transparency sheets and imaged on a ChemiDoc Touch Imaging System (Bio-Rad Laboratories) using Immobilon western chemiluminescent HRP substrate (MilliporeSigma ...
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bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
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bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
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bioRxiv - Plant Biology 2020Quote: ... and stopped at each of the above-mentioned time points by loading an 150 µl aliquot of the reaction mixture to an anion exchange resin column (acetate form, 100-200 mesh, Dowex AG1-X8 Resin, Bio-Rad, UAS). The resin column was previously equilibrated five times using 150 mM sodium acetate (pH=7.5) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were eluted in SDS sample buffer and 30 μl of each sample was run on a 12% Bis-Tris 1D SDS-PAGE gel (Biorad) for 1 cm and stained with colloidal Coomassie dye G-250 (Gel Code Blue Stain Reagent ...
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bioRxiv - Neuroscience 2021Quote: Samples were resolved by SDS-PAGE using either 10 or 12% acrylamide/bis-acrylamide gels and transferred to 0.22 µm nitrocellulose (Bio-Rad). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... Running buffer was prepared with tris-glycine 1X and SDS 0.1% using a homemade gel (7-12% bis-acrylamide, Bio-Rad), which ran at 100 V for approximately 2:30 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 or 12.5% bis-tris gels and protein was transferred to PVDF membranes using a wet transfer system (Bio-Rad). Membranes were blocked with 5% milk in TBS-T and incubated overnight with primary antibody in either milk or BSA at manufacturer recommended concentrations ...
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bioRxiv - Microbiology 2020Quote: One hundred μg of the different PAO1 cytoplasmic extracts were loaded onto conventional SDS-PAGE 10% Bis-Tris gels (mini-protean TGX Stain-free precast Gels, BioRad). The gel was stained with Coomassie blue and each lane was cut into 10 bands ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were heated at 95°C for 5 min and separated on a 12% Bis-Tris SDS-PAGE gel (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... Samples were resolved by SDSPAGE using 10% acrylamide/bis-acrylamide gels and transferred to 0.2 μm pore size nitrocellulose (Bio-Rad). Membranes were blocked with Odyssey blocking buffer (LI-COR ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated eMAP hydrogel monomer solution (30% acrylamide [A9099, MilliporeSigma, St. Louis, MO, USA], 10% sodium acrylate [408220, MilliporeSigma], 0.1% bis-acrylamide [161-0142, Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: Samples were resolved by SDSPAGE using either 10 or 12% acrylamide/bis-acrylamide gels and transferred to 0.22 µm nitrocellulose (Bio-Rad). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were deposited onto 26-well 12% Bis-Tris Criteroin XT precasted gels (Bio-Rad Laboratories Inc., Hercules, CA, USA) and electrotransferred onto a nitrocellulose membrane with a semidry electrotransfer system (Schleicher & Schuell BioScience ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were loaded onto 10% bis-Tris gels and transferred to Immun-Blot PVDF membranes (1620177, Bio-Rad, Hercules, CA), which were then blocked in 5% blocking grade skim milk (170–6404 ...
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bioRxiv - Neuroscience 2024Quote: Samples were resolved by SDS-PAGE using 12% acrylamide/bis-acrylamide gels and transferred to 0.22 µm nitrocellulose (Bio-Rad). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well (ThemoFisher, NP0321BOX) and transferred to polyvinylidene fluoride (PVDF) membranes (Biorad, 1704156EDU). PVDF membranes were blocked with 5% nonfat dried milk in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2024Quote: ... Spinal cord ‘myelin’ and optic nerve samples were resolved on 4-12% Bis-Tris geld at 200 V and transferred onto PVDF membrane using either semi-dry method (Trans-blot; BioRad) or a wet gel transfer (Mini Trans-blot ...
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bioRxiv - Biochemistry 2024Quote: Unpolymerized 10% Tris-HCl gel solution (250 mL) was prepared by combining 30% bis/acrylamide (83.33 mL, #1610156, BIO-RAD), 1.5M Tris-HCl ...
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bioRxiv - Pathology 2024Quote: ... Western blots were run with non-reducing conditions using precast 10% Criterion XT Bis-Tris polyacrylamide gels (3450112; Bio-Rad) and MOPS buffer (1610788 ...
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bioRxiv - Biophysics 2024Quote: Claudins purified as described above were exchanged from DDM to 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG) detergent via a PD-10 column (Bio-Rad). COP-1 was added in 1.4 moles excess to claudins then 1.4 moles excess Nb to COP-1 were mixed and incubated at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... the ratios of acrylamide to bis-acrylamide in solution were 7.5% acrylamide (40% w/v solution, Bio-Rad, Hercules, CA) and 0.25% N,N′-methylene-bis-acrylamide (2% w/v solution ...
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bioRxiv - Bioengineering 2024Quote: ... and interleukin 4 (IL-4)) cytokines run on a Bio-Plex 200 reader (BioRad). Data was collected from only two donors for RANTES ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4970) overnight at 4 °C and the HRP-conjugated secondary antibodies (BIO-RAD, Cat. No. 1706515, 1:1000) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunostaining was performed overnight at 4° C using primary antibodies for PTH (1:400, Bio-Rad, Hercules, CA), Cleaved caspase 3 (1:200 ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated on 12% SDS-polyacrylamide (29:1) or 4-15% precast polyacrylamide mini-gels (Bio-Rad) and electrotransferred on nitrocellulose membranes ...