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Citations for Bio-Rad :
5801 - 5850 of 7983 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Complementary DNA (cDNA) was generated from 1 µg total RNA using the iScript cDNA Synthesis kit (Bio-Rad, 1708841). Primers were designed with NCBI Primer-BLAST ...
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bioRxiv - Cancer Biology 2024Quote: ... The following secondary antibodies were used: anti-rabbit IgG conjugated to HRP (Goat, 1:3000, #170-6515, Bio-Rad), anti-mouse IgG conjugated to HRP (Goat ...
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bioRxiv - Cancer Biology 2024Quote: ... were applied for one hour at 1:10,000 dilution and proteins were detected using Clarity Western ECL Substrate (BioRad). Secondary antibodies were goat anti-rabbit IgG HRP (1:10,000 ...
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bioRxiv - Genetics 2024Quote: ... samples were heated at 95°C for 4min and separated on 15% denaturing polyacrylamide gels (ratio acrylamide:bis acrylamide 19:1 [Biorad]). After fixing in a solution (40% methanol ...
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bioRxiv - Developmental Biology 2024Quote: ... and cDNA was synthesized from 1 μg of RNA using the iScript™ Reverse Transcription kit (Bio-Rad, USA). qRT-PCR was performed using gene-specific primers for eef1a1l1 ...
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bioRxiv - Biophysics 2024Quote: ... 5% acrylamide (BIORAD), 0.1% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was prepared for western blotting by diluting 20 μg of protein with 1 × Laemmli buffer (Bio-Rad #1610747) and ultrapure water (Invitrogen #10977015 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined at indicated time points by adding 1/10 volume AlamarBlue solution (Biorad, Hercules, CA, United States) to each well ...
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bioRxiv - Cell Biology 2020Quote: ... and equal amounts (1–2µg) were used for reverse transcriptase reaction using an iScript™ Reverse Transcription Supermix (Bio-Rad). qPCR was run using the SYBR® Green Master Mix on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were co-encapsulated with Drop-Seq beads (ChemGenes: lot 083117) in 1 nL droplets using 15000 mL/hr oil (QX200, Biorad), 4000 mL/hr cell suspension and 4000 mL/hr bead flow rates (droplet generation frequency 2 kHz) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Cell Biology 2020Quote: ... incubating with HRP conjugated secondary antibodies (1:5000) and developing with Clarity™ Western ECL blotting substrate (Bio-Rad, 1705061) and imaging with X-ray film ...
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bioRxiv - Genetics 2021Quote: ... Proteins were visualized using horseradish peroxidase-conjugated goat anti-rabbit secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706515) or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated with ECL Western Blotting Substrate (Thermo, 32106) for 1 minute prior to imaging with the ChemiDoc system (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µg of cleaned RNA was used to generate cDNA with iScript reverse transcriptase per the manufacturer’s protocol (Bio-Rad). 20 µL quantitative real-time PCR reactions with 100 ng of cDNA input were carried out using TaqMan Fast Advance Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with random primers using an iScript cDNA synthesis kit (Bio-Rad) and analyzed with a Bio-Rad CFX96 real time system ...
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bioRxiv - Immunology 2022Quote: ... 1-0.2 μg of RNA was used to synthesize cDNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA template was mixed with iTAQ SYBR green universal super mix (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were rinsed several times in 1xTBSTw and incubated with goat anti-rabbit HRP-conjugated secondary antibody (1:20,000; Bio-Rad, catalog 179-5046 ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with NaIO4 for 1 h then 15 min with NaS2O3 and mixed with 100 μl AffiGel Hz Hydrazide Gel (Biorad) overnight ...
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bioRxiv - Microbiology 2020Quote: ... The gel was run for 1 h at 100 V and visualized on a ChemiDoc MP Imaging System (BioRad, Germany).
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bioRxiv - Cancer Biology 2020Quote: ... ran at 120 V for ∼2 h and transferred at 100 V for 1 h onto nitrocellulose membranes (Bio-rad) using the wet blot method ...
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bioRxiv - Plant Biology 2020Quote: ... was used at a dilution of 1:2,000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:2,000) followed by a chemiluminescence reaction (Clarity Western ECL Substrate; Bio-rad). For the SS-TOM lines analysis ...
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bioRxiv - Neuroscience 2020Quote: ... RNA (1 μg) was reverse-transcribed to cDNA with the iScript ™ cDNA Synthesis Kit (Bio-Rad Laboratories, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Gel was stained for 1 h with 0.1 μg/ml ethidium bromide and visualized by ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (1: 8000; Cat. 170-6516, Bio-Rad, Milan, Italy) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Detergent was removed by three addition of BioBeads SM-2 adsorbent resin (25 mg per 1 mL of lipid mixture) (BioRad) for 2 h each at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 15% for FKB-2) and transferred onto nitrocellulose membranes for 1 hour at 200 mA (SemiDry transfer blot, Bio-Rad). After incubation with blocking solution (LICOR Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was diluted 1:100 and real-time PCR was performed in triplicate with iQ SYBR Green Supermix from BioRad.
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was synthesized from 1 μg of isolated RNA using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05 ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was let dry and thereafter blocked on a shaker for 10 min in 15 ml blocking buffer (1% casein in TBS, BioRad). The blocking solution was discarded ...
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bioRxiv - Neuroscience 2020Quote: ... We used 1 microgram of the total RNA for cDNA synthesis (iScript cDNA Synthesis Kit, Cat. #170-8891, Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane was treated with ECL (Immobilon Western chemiluminescence HRP substrate) for 1 min and visualized with the ChemiDoc MP imaging system (BioRad). Exposure was optimized for each blot.
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bioRxiv - Microbiology 2020Quote: ... Visualisation and quantification were carried out using a Personal Molecular Imager with Quantity One 1-D analysis software (Bio-Rad) and data were analyzed with GraphPad Prism 4 software.
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bioRxiv - Plant Biology 2020Quote: ... and cDNA was synthesized from 1 µg DNA-free RNA using the iSCRIPT cDNA synthesis kit (BioRad, Hercules, CA, U.S.A). Real-time PCR master mixes were prepared using iQ sybr Green Mix (BioRad ...
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bioRxiv - Systems Biology 2020Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... 15 μL of each reaction was immediately loaded onto an 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad) supplemented with 2 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 μl of ChIP-ed or input (diluted 30-fold to bring it to 1%) DNA was used in 20 μl total volume reaction using SYBR green master mix (BioRad) and 0.4 μM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5,000, Bio-Rad 170-6515) or goat anti-mouse immunoglobulins (1:5,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 µg of total RNA was reverse transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Feldkirchen, Germany). Real-time PCR was performed using Platinum SYBR Green qPCR Super Mix-UDG (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The following day the membrane was washed with PBST twice for 10 minutes and incubated with secondary antibodies diluted in the same solution (1:10000 HRP-conjugated anti-mouse or anti-rabbit antibodies (Biorad)) in the same solution at room temperature for 45 minutes ...
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bioRxiv - Genetics 2021Quote: ... Secondary antibodies used were: horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG secondary antibody (1:5000, BioRad). Blots were visualized using chemiluminescence (Clarity Max™ ECL ...
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bioRxiv - Cell Biology 2021Quote: ... The Taqman probes (Suppl Table 1) used were the following: droplets were generated with a QX100 droplet generator (Bio-Rad), after mixing 20 µl of reaction mix and 70 µl of droplet generator oil (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
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bioRxiv - Cell Biology 2020Quote: ... Goat α-mouse secondary IgG-HRP antibody was used at a 1:5,000 dilution (BioRad Laboratories, Hercules, CA, 170-6516). Phospho-MAPK antibodies were incubated in 1X TBST (10 mM TRIS-HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: The isolated total RNA (1 μg) was reverse-transcribed to produce cDNA using iScript Reverse Transcription Supermix kit (Bio-Rad). Real-time PCR was performed using SYBR Green supermix (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on native acrylamide gels containing 0.5X TBE buffer and 7% acrylamide (made from a stock of 40% acrylamide with a ratio of 29:1 acrylamide:bisacrylamide; Bio-Rad). The running buffer for the gels was 1XTBE ...