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Citations for Bio-Rad :
5701 - 5750 of 6467 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The Taqman probes (Suppl Table 1) used were the following: droplets were generated with a QX100 droplet generator (Bio-Rad), after mixing 20 µl of reaction mix and 70 µl of droplet generator oil (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
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bioRxiv - Cell Biology 2020Quote: ... Goat α-mouse secondary IgG-HRP antibody was used at a 1:5,000 dilution (BioRad Laboratories, Hercules, CA, 170-6516). Phospho-MAPK antibodies were incubated in 1X TBST (10 mM TRIS-HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: The isolated total RNA (1 μg) was reverse-transcribed to produce cDNA using iScript Reverse Transcription Supermix kit (Bio-Rad). Real-time PCR was performed using SYBR Green supermix (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on native acrylamide gels containing 0.5X TBE buffer and 7% acrylamide (made from a stock of 40% acrylamide with a ratio of 29:1 acrylamide:bisacrylamide; Bio-Rad). The running buffer for the gels was 1XTBE ...
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bioRxiv - Pathology 2022Quote: ... The CD68 primary antibody incubation was performed at room temperature for 2 hours (mouse CD68, BIO-RAD, MCA1957, 1:750) and CD11b staining (BD Pharmigen ...
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bioRxiv - Cancer Biology 2022Quote: ... and the pellet was resuspended in 1 ml of BM1* and cells were counted using a TC20 Automated Cell Counter (BioRad). Single-cell suspensions were mostly re-seeded at the same density as their parental/previous generation/passage.
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bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated anti-mouse was incubated for 1 h at room temperature and visualized by enhanced chemiluminescence (Clarity Substrate, Biorad) using Amersham Imager 680.
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bioRxiv - Biophysics 2022Quote: ... The protein sample was incubated at 40°C and 80 °C respectively for 1 h in a PCR cycler (BioRad) with a heated lid to prevent evaporation ...
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bioRxiv - Bioengineering 2022Quote: ... Tricine-SDS-PAGE experiments were performed using a single layer 0.75 mm thick 20% acrylamide/bis-acrylamide gel (using 29:1 Acrylamide/Bisacrylamide, Bio-Rad) run at 120 V constant voltage (Pharmacia LKB ...
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bioRxiv - Cancer Biology 2022Quote: ... Up to 1 µg of total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) or gDNA Clear iScript cDNA Synthesis Kit for patient-derived tumor cells (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 1 μg of purified RNA using the iScript cDNA synthesis kit (Cat. no. 1708891, Bio-Rad). Quantitative real-time PCR assays were performed using the iQ SYBR Green Supermix (Cat ...
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bioRxiv - Biochemistry 2021Quote: ... The same samples were also detected by HRP conjugated StrepTactin antibody at 1:10000 dilution (Bio-Rad catalog number 161038). To allow detection of endogenous selenok ...
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bioRxiv - Genomics 2020Quote: ... Membranes were washed using tris-based saline buffer solutions with 0.1% tween-20 (TBST) and blocking solutions were prepared using 5% w/v western-blot grade dry milk powder (Biorad). Membranes were blocked with blocking buffer for 1 hour and washed between incubations for 5-10 mins (3x) ...
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A gut-secreted peptide controls arousability through modulation of dopaminergic neurons in the brainbioRxiv - Neuroscience 2020Quote: ... DNA product was diluted 1:10 and used to set up quantitative PCR reactions using Sybr Green (Biorad #170-8880). Primers were obtained from ID Technologies:
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bioRxiv - Molecular Biology 2020Quote: ... 1 µg of RNA from each sample was reverse-transcribed in 40 µl using iScript™ cDNA Synthesis Kit (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... in TBS-T (TBS supplemented with 0.1% (v/v) Tween-20) and 1% (w/v) Blotting-Grade Blocker (Bio-Rad) at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:2,000 dilution and HRP-conjugated goat anti-mouse secondary antibody was used in a 1:3,000 dilution (172-1011, Bio-Rad). Following gDNA based PCR screening and Western blotting ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μL of electrocompetent cells were transformed with 1 ng of the lesion-containing plasmid mixed with 1 ng of pVP146 (internal control for transformation efficiency) by electroporation using a GenePulser Xcell (BioRad) at 2.5 kV ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were washed 3 times for 5 minutes with TBS-T prior to incubation in ECL reagent for 1 minute and imaging using a Chemidoc MP (BioRad). Images acquired were analyzed using Image Lab software (BioRad).
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bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
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bioRxiv - Genetics 2022Quote: ... followed by goat anti-mouse IgG (H+L) alkaline phosphatase (AP)-conjugated (1:3000, Bio-Rad Cat# 170-6520, RRID:AB_11125348) antibody or Alexa Fluor 546 goat anti-mouse IgG (H+L ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The mixture was transferred to an electroporation cuvette with a 1 mm gap width and transformed using a Gene Pulser Xcell electroporator (BioRad) with 1.8 kV ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Pathology 2022Quote: ... with 1% Triton X-100] and the protein concentration of each lysate was determined with a colorimetric assay (Bio-Rad). After mixing with SDS-PAGE sample buffer ...
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bioRxiv - Pathology 2022Quote: ... 1 or 10 μg of total protein were loaded and separated by SDS–PAGE on 10% mini gels (Bio-Rad), and transferred into a 0.2 μm-pore size nitrocellulose membrane (#GE10600001 ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies (for further specification see key resource table, table 1) were diluted in blocking solution (F4/80 [Bio-Rad] ...
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bioRxiv - Pathology 2020Quote: ... both used at 1:1,000 dilution in blocking buffer) and fluorescent signals were acquired using with a GelDoc Imager (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
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bioRxiv - Developmental Biology 2021Quote: ... was performed using 1 μl of cDNA as a template with qPCR primers (Table 2) and iTaq Universal SYBR Green Supermix (Biorad) according to the manufacturer’s instruction and analyzed using CFX Connect Real Time System (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosomal DNA was separated in 1% agarose/0.5 × TBE on the CHEF-DR II Pulsed Field Electrophoresis Systems (Bio-Rad) as follows ...
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bioRxiv - Immunology 2020Quote: ... Purified libraries were diluted 1:100 and quantified again by qPCR (Two 25uL reactions, 2x iQ SYBR SUPERMix (Bio-Rad, with Read 1 (5’-TATGGTAATTGT GTGYCAGCMGCCGCGGTAA-3’) ...
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bioRxiv - Pathology 2020Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Physiology 2021Quote: ... Membranes were washed with 4 x 15 min TBS-T washes prior to incubation with secondary antibody (goat anti-rabbit-HRP diluted 1:10,000, Bio-Rad) for 1 h on a shaker at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 40S and 80S fractions were quantified using Quantity One 1-D Analysis Software v.4.1.2 (Bio-Rad Laboratories, Hercules, CA). Pno1 was quantified using ImageJ Software v1.52 (100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with secondary antibody for 1 h and proteins were revealed by ECL Prime (Amersham) using ChemiDoc Touch imager (Biorad).
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bioRxiv - Biochemistry 2020Quote: ... Samples (1 µg total protein) were loaded onto non-reducing 12% Mini-PROTEAN® TGX™ precast gels (Bio-Rad), and molecular weight shifts and relative band intensity used to detect the presence and relative abundance of the crosslinked complex ...
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bioRxiv - Microbiology 2020Quote: ... glycine and 0.1% (w/v) SDS in distilled water) for 1 hour at 150 volts (Mini-protean vertical electrophoresis cell, Bio-Rad). The gels were stained in Coomassie stain (45% methanol ...
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bioRxiv - Genetics 2020Quote: ... and the absorbance was monitored at 254 nm with an ultraviolet absorbance detector (Biorad model EM-1 Econo UV monitor) to record the polysome profile ...
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bioRxiv - Genetics 2020Quote: ... Each 20 microliter reaction volume contained the equivalent of 2.0 ng RNA of the template cDNA and 200 nanomoles of each primer in 1 X SYBR Green Supermix (BioRad). The cycling conditions included one step at 95°C for 3 min ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were then washed 3x with TBS-T for 10 min each and incubated with the horseradish peroxidase (HRP) conjugated specific secondary antibodies (1:20,000, Bio-Rad) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with Amersham ECL Western Blotting Detection Reagent (Global Life Science Solutions) for 1 min and imaged on a ChemiDoc Imaging System (BioRad).
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were further incubated in peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000) (BioRad, Hercules, CA, USA) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Staining was removed by washing the membrane in TBS-T and the membrane was blocked for 20 min using 1% Casein in TBS (BioRad). To detect PAR chains with incorporated 6-Biotin-17-NAD+ (Biolog) ...
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bioRxiv - Biochemistry 2021Quote: A 9.5% gel solution was prepared by combining 47.5 mL 30% Bis/Acrylamide solution 29:1 (BIO-RAD, #161-0156) with 41mL MilliQ Water ...
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bioRxiv - Cancer Biology 2021Quote: Click reaction mixtures and unreacted alkyne-modified oligonucleotides were separated in a 1.2 % agarose gel for 1 h at constant 120 mV and DNA bands were captured by the ChemiDoc system (BioRad) using SYBR safe mode.
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bioRxiv - Molecular Biology 2021Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h at RT with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad). After the same washing procedure ...
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bioRxiv - Neuroscience 2021Quote: Recombinant or mouse-extracted tau fibrils were diluted in PBS as indicated in Supplementary Figure 1 and applied to 0.2 µm nitrocellulose membrane using the Bio-Dot microfiltration apparatus (Bio-Rad). The membranes were then blocked in 5% milk TBS-Tween 20 and subsequently incubated with primary antibody overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were scanned the following day after 1 hour incubation with secondary antibodies using Odyssey CLx Imaging System or ImageQuant system (BioRad, HRP anti-rabbit or anti-mouse (CST ...