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Citations for Bio-Rad :
5651 - 5700 of 6365 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Microbiology 2022Quote: ... in 1x TBS buffer for 30 minutes at 250 V and transferred using the Trans-Blot turbo Mini 0.2 μm PDVF transfer kit (Bio-Rad) with a Transblot Turbo transfer system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 50 μL of 1X PBS and protein concentration was using The DC Protein Assay Kit (Biorad).
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bioRxiv - Microbiology 2022Quote: ... on cDNA samples using the LightCycler 480 SYBR Green I Master kit in the CFX384 RT-PCR Detection System (Biorad). The identities of the resulting amplicons were checked by melting-curve analysis using the CFX384 software ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng DNase-treated RNA was used as template for cDNA synthesis using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad). The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... The cDNA utilized in the PCR experiments was generated via the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules California #1708890). PCR Reactions were then performed in triplicate (3n ...
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bioRxiv - Neuroscience 2023Quote: ... a cDNA library was generated using 500 ng of total RNA using the iScript cDNA synthesis kit from Bio-Rad. The concentration of the cDNA was then quantified using the Qubit 2.0 assay for oligos per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were hybridised with an appropriate DNA probe— as indicated in figure legends and Table S2—radiolabelled with P32 prepared via random priming using the High Prime kit (BioRad). Pre-hybridisation solution was discarded ...
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bioRxiv - Physiology 2023Quote: ... was reverse transcribed and converted to complementary DNA (cDNA) using the iScript cDNA synthesis kit (1708891, Bio-Rad, Hercules, CA). Each cDNA sample was amplified with messenger RNA (mRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... the pellets were re-dissolved in 1 M NaOH and quantified by Bradford assay (Quick StartTM Bradford Kit, BIO-RAD), and the supernatants containing cADPR were neutralized with Chloroform/Tri-n-octylamine mixture (volume ratio 3:1 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of total RNA were treated with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; https://www.bio-rad.com/) for genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Cell Biology 2022Quote: ... First strand cDNA was reverse transcribed from equal amounts of total RNA using iScript cDNA synthesis kit (Biorad, Hercules, CA). Specific primers for amplification of the mouse Ctnna3 message ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: cDNA synthesis was performed on extracted mRNA as described in (Mohan et al., 2019) or according to manufacturer’s protocol using the iScript cDNA Synthesis Kit (Bio-Rad). SYBR qPCR was performed as described in (Mohan et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 1 µg RNA was used for cDNA synthesis with the IScript cDNA Synthesis Kit (Bio-Rad, 1708890), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... iScript Advanced cDNA Synthesis Kit (Cat# 172-5037) and ddPCR Supermix for Probes (186-3026) were obtained from Bio-Rad. PCR primers listed in table S3 were obtained from Integrated DNA Technologies.
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bioRxiv - Physiology 2024Quote: ... Total RNA (1 μg) was reverse transcribed to cDNA using an iScript cDNA synthesis kit (Cat no. 170884, BioRad, USA), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was transcribed into cDNA using the “iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR” (Bio-Rad, Hercules, CA, USA). The real-time PCR was performed with the “SsoAdvancedTM Universal SYBR Green Supermix” (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... 1000 ng of RNA was used for reverse transcription in a 6.5 µl reaction using the iTaq Universal SuperMix Two-step kit (Biorad, 1725130). 3 µl of the master mix for each probe and 2ul of the generated cDNA was pipetted into a 384-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations of brain and olfactory extracts were determined using the RC DC Protein Assay kit (Bio-Rad, Hercules, CA) as described (Miller et al. ...
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bioRxiv - Physiology 2024Quote: ... 450 ng of mRNA from Quad (Fig. 1A) or PLA (Fig. 2C, Myc only) muscle were reverse transcribed using the iScript cDNA Synthesis kit (Biorad). Next ...
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bioRxiv - Microbiology 2024Quote: ... Transfer was done with the Transblot Turbo RTA transfer kit Nitrocellulose and detection was performed with the Clarity Max Western substrate (Biorad) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and 100ng of RNA was used with iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad, CA, USA) to perform a one-step RT-qPCR in a volume of 20 μl using a QuantStudio3 real-time PCR instrument from ThermoFisher (Waltham ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse transcribed to generate cDNA using the iScript Reverse Transcriptions SuperMix for RT-qPCR kit (Bio-Rad Laboratories). Real-time PCR was performed using the SYBR select Master Mix for CFX (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a PVDF membrane using the Bio-Rad Turbo Transblot System and transfer kit (Bio-Rad Laboratories) and fixed in methanol prior to blocking in 5% non-fat milk in TNT buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of up to 1μg of total RNA was performed using the iScript cDNA Synthesis Kit (Bio-Rad, #1725038).
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bioRxiv - Neuroscience 2023Quote: ... High integrity RNA (RIN > 7) was used to synthesize cDNA with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). PCR primers for human B-ACTIN ...
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bioRxiv - Biophysics 2023Quote: ... proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 250-1000 ng of total RNA using an iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Quantitative PCR for each candidate gene was performed using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were assessed using the Protein Assay BCA kit (Nacalai Tesque) with bovine serum albumin (Bio-Rad, Hercules, CA) as a standard ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA samples from monotreme tissues were reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). RNA was stored in nuclease free water at -80°C.
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bioRxiv - Immunology 2024Quote: ... RNA from 14 3h PBF guts was transcribed into cDNA with the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad) and RT-PCR was performed with RedTaq Polymerase (VWR ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA cDNA synthesis kit (BIORAD, CAT#1708891), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Conversion of one µg of RNA into cDNA was performed using the iScript cDNA Synthesis Kit (1708890; Bio-Rad Laboratories) and Master Cycler Pro Device (EPPE6324000.516 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membrane strips were finally washed 6x for 3mins in 1X TBST before being developed with the ECL Clarity Kit (BioRad) and imaged on ChemiDoc Imaging system (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA retro-transcription was performed using a High-Capacity cDNA Reverse Transcription Kit on a MyCycler (Bio-Rad, Hampton, NH). Next ...
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bioRxiv - Pathology 2023Quote: ... Proteins were resolved on 4-15% SDS-PAGE gels and transferred to nitrocellulose membrane (Trans-Blot Turbo RTA transfer kit, #1704271, Biorad). Membranes were blocked for 1h at room temperature in 5%BSA-TBS-T (TBS-0.1%Tween 20 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were resolved on 4-15% SDS-PAGE gels and transferred to nitrocellulose membrane (Trans-Blot Turbo RTA transfer kit, #1704271, Biorad). Membranes were blocked for 1h at room temperature in 5% BSA-TBS-T (TBS-0.1%Tween 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... transferred to nitrocellulose membranes using semi-dry transfer apparatus and Tans-Blot Turbo RTA Midi Nitrocellulose Transfer kit (Bio-Rad). The membranes were then blocked with 3% milk in TBST ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibody was washed away 5× with TBS-T and developed with an AP Conjugate Substrate Kit (Bio-Rad). Spots were quantified using a Nikon ELISPOT system and NIS-Elements AR software (v ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from sorted cardiac cells or from cardiac tissues (atria or ventricle) using the Aurum total RNA fatty and fibrous tissues kit (Biorad) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaqTM universal SYBR® Green One-Step kit (Bio-Rad): 95 °C for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... A 1.0 μg of total RNA was used for the cDNA synthesis using the iScript cDNA Synthesis kit (Bio-Rad). The qRT-PCR analysis was performed using CFX 384 Touch RT-PCR detection system with a reaction mixture of 2.0 μl of primer mix (2.0 μM from each F & R primer) ...
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bioRxiv - Physiology 2023Quote: ... 500 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad #1708891, Hercules, CA, USA) per the manufacturer’s instructions ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... interleukins and ghrelin were measured using Biolpex multiplex kits (cat# M50-0KCAFOY and cat # 171-A4S01M, Bio-Rad, CA, USA). Standards with known concentrations of specific analytes were reconstituted and diluted as instructed in the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... tagmentation and droplet library preparation were performed following the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... qPCR was performed using the SYBR Green Supermix Kit (Vazyme) on the CFX96 Real-Time PCR System (Bio-Rad, USA). The expression of target genes was normalized to expression of Gapdh ...
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bioRxiv - Microbiology 2023Quote: ... Separated proteins were then transferred to a nitrocellulose membrane using a Trans-Blot Turbo RTA Mini 0.2 μm Nitrocellulose Transfer Kit and Trans-Blot Turbo system (Bio-Rad). The membrane was cut to separate the regions above and below approximately 70 kDa ...
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bioRxiv - Microbiology 2023Quote: ... first-strand cDNA synthesis was prepared from 200 ng of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad), according to the manufacturer’s instructions ...