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Citations for Bio-Rad :
5551 - 5600 of 6469 citations for 4 Bromo 4' Butyl 1 1' Biphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription of 1 μg RNA/20μl was performed with the iScript™ cDNA Synthesis Kit (1708890, Bio-Rad). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (1725120 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with respective anti-mouse or anti-rabbit HRP-conjugated secondary antibody (1:1000, Bio-Rad) and visualized with a chemoluminescence kit (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... which was inoculated at 30°C for 1 hour and then photographed using a UV transilluminator (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... Amplification products were resolved in 1% (w/v) agarose gel and analysed with GelDoc XR+ System (Bio-Rad Laboratories). Primers and probes are listed in Table S1.
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were detected with anti-mouse HRP-conjugated secondary antibodies (1:5000; Bio-Rad, Hercules, CA, USA), and the signal was developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
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bioRxiv - Microbiology 2020Quote: ... 15 % methanol (v/v) in distilled water) for 1 hour at 100 volts (Mini Trans-Blot Cell, Bio-Rad). The nitrocellulose membrane was blocked in blocking buffer (5% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Complementary DNA was synthesized from 1 μg total RNA with an iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). A CFX 96 Connect™ system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the Amylose resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were washed in TBS-Tween and incubated with the peroxidase-conjugated secondary antibody (Bio-Rad, Table 1) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... the Netherlands) and blocked for 1 hour at room temperature using Odyssey blocking buffer (LI-COR biosciences, Bio-Rad) in a 1:1 dilution with tris-buffered saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... 1 ug of mRNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA). RNA was hydrolyzed and re-suspended in nuclease free water ...
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed with TBST and incubated with StarBright Blue 700 Goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 hour ...
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bioRxiv - Genetics 2023Quote: ... ∼25 μL sample was run out on a 7.5% SDS-PA at 150V for ∼1 h alongside a protein standard (BioRad Precision Plus Protein Dual Color Standard) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was normalized to 1 mg/mL using 0.1% Azo buffer by the Bradford assay (Bio-Rad). Samples were reduced with 30 mM DTT for 1 h and subsequently alkylated with 30 mM iodoacetamide for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used were the following: HRP-conjugated goat anti-mouse IgG (1:10000, Bio-Rad, 170-6516); HRP-conjugated goat anti-rabbit IgG (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Axonal staining and myelination in the SLM were assessed with rat anti MBP (BIO-RAD, cat # MCA409S 1:500) and mouse anti-Neurofilament H (NF-H) ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked 1 hr with 5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad, Cat# 1706404XTU) and incubated overnight with the primary antibodies [TTC3 Polyclonal Antibody (Abcam ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1×108 concentrated fungal protoplasts (37 μL) (64) were mixed with 2% CleanCut Agarose (63 μL, Bio-Rad, #1703594). The mixture was then loaded into a plug mold (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Thawed lysates were run on 7.5% Acrylamide gels with a 29:1 ratio Acrylamide:Bis using a Protean Tetra system (Bio-Rad) and Precision Plus markers (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using RNAeasy kit from Qiagen (#74104) and 1 ug of RNA was used to synthesize cDNA using iScript cDNA Synthesis Kit (#1708891) from BioRAD. qRT-PCR was performed using TaqMan universal PCR mix (#4324018 ...
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bioRxiv - Microbiology 2023Quote: ... diluted to 20 ng/µL in molecular grade water and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). Relative expression was calculated by ΔΔCt method to GapDH 66 ...
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bioRxiv - Developmental Biology 2023Quote: ... and cDNA was synthesized from 1 µg of RNA using the iScript™ Reverse Transcription kit (Bio-Rad, USA). qRT-PCR was performed using gene-specific primers for eef1a1l1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative Real rime PCR (RT-qPCR) analysis was performed on genes of interest (Supplementary Table 1) using SYBRGreen (BioRad). Sso Fast EvaGreen Supermix was performed to analyze gene expression on a BioRad CFX96 Real-Time System (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... Complementary DNA was then synthesized from 1 μg of total RNA using iScript cDNA Synthesis Kit (1708890, Bio-Rad) and Bio-Rad T100 thermocycler ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was diluted 1 in 10 and 4µl of diluted supernatant was added to 200µl of Bradford reagent (Bio-Rad) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug of RNA was diluted into 15 uL reverse transcription reactions using the iScript RT kit (Bio-Rad) and reactions were carried out following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: After washing with TBS-T 0.1% membranes were incubated with goat anti-mouse (1:1000, Bio-Rad, Madrid, Spain), goat anti-rabbit (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... For quantitative real-time PCR 20 ng cDNA and 1 pmol/μl primers were mixed with 2x SYBR (BioRad) in a total volume of 13 μl for each well ...
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bioRxiv - Microbiology 2023Quote: ... in 1×TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo Transfer System (BioRad). Blocking was performed in a 5% solution of dry milk and 0.05% Tween 1×PBS (1×PBST ...
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Diluted mouse serum (1:100 in 0.1% BSA in PBS) was incubated on HEp-2 slides (MBL or BioRad) for 1 hour in the dark at room temperature ...
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bioRxiv - Genomics 2023Quote: ... and 1 µg of RNA was used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, #1708890). We used RT-qPCR to quantify MYC expression (forward primer ...
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bioRxiv - Biophysics 2023Quote: ... 50 mM Tris pH 7.4 was added to quench the reaction and the samples were buffer exchanged into BLI buffer (20mM Tris pH 7.4, 100mM NaCl, 1% glycerol, and 0.03% DDM) using PD-10 columns (Bio-Rad). For enterotoxins ...
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bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed on 1 μg of RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). Then ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli and plasmid DNA were electroporated in 1 mm cuvettes at 1.8 kV using the MicroPulser electroporator (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... We synthesized the first strand cDNA with 1 µg of total RNA using iScriptTM Reverse Transcription Supermix (BIO-RAD) followed by quantitative PCR with CFX96 Real-Time System (BIO-RAD ...
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bioRxiv - Cancer Biology 2024Quote: Cells were first embedded in gel plugs with approximately 1 million cells per gel plug (BioRad no. 170-3592). The high molecular weight genomic DNA was extracted and purified using the Bionano Genomics SP kit.
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bioRxiv - Microbiology 2024Quote: ... Cell lysates and purified supernatants were separated on an SDS-PAGE and analyzed through immunoblotting against vinculin as a loading control (1:2,000, MGA465GA, BioRad) or CHIKV E1 glycoprotein (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of the RNA was reverse transcribed into cDNA with the iScript Advanced cDNA Synthesis kit (Bio-rad). 20 μL of the end products were diluted by adding 180 μL nuclease-free water and stored at -20 °C until further analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated goat anti-rabbit IgG (1:5000; 1706515, Bio-Rad) at RT for 1 h followed by chemiluminescent detection with ECL Substrate (1705060 ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription (RT) was performed on 1 µg RNA with the iScript™ Reverse Transcription Supermix (Bio-Rad 1708841) for 5 min at 25°C ...
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bioRxiv - Genomics 2024Quote: ... F43E2.6 siRNA and miR-1 Ct values were assayed using a CF96 Real-Time PCR Detection System (Bio-Rad). Ct values were averaged across three technical replicates for each of 3 biological replicates.
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bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
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bioRxiv - Cell Biology 2024Quote: ... and goat anti-rabbit (Cat. # 170-6515) antibodies were used at 1:2000 for immunoblotting and purchased from Biorad.