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Citations for Bio-Rad :
501 - 546 of 546 citations for Respiratory Syncytial Virus A Glycoprotein G Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts (50 μg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Immunology 2021Quote: We mixed 500 μg of extracted cell lysates and 100 μL of patients’ sera with 100 μL of SureBeads™ Protein G Magnetic Beads (Bio-Rad, Hercules, CA) and added phosphate-buffered saline containing 0.2% of Tween 20 (PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts (50 µg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cell protein lysate was pre-cleared with 25 μL of SureBeads TM Protein G magnetic beads (BIO-RAD, Catalog number – 1614023) for 1 hour at 4 ºC and the beads were removed three times using a magnetic stand ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg of dsDNA were added to the competent cells and then transferred to chilled 2 mm gap electroporation cuvettes (Bio-Rad Laboratories GmbH, Germany). Electroporation transformation was done with a single pulse at 1.8 kV ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: The collected fractions were then pooled by the expected oligomeric state for both human milk and serum and again IgAs were captured on beads with a 1% blocking buffer background (Bio-Rad, Netherlands) and addition of the 7D8-IgA1 and 5D5v2-IgA1 at 0.2 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... TNF-β were detected by using the premixed 48-plex Bio-Plex Pro Human Cytokine Assay (Bio-Rad, Hercules, CA, USA; 12007283) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: Human cytokines in the serum were measured by Bio-plex Pro TM Human Cytokine Screening 48 plex Bio-PlexTM 200 System (# 12007283, Bio-Rad, US) and Bio-PlexTM 200 System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: Human and murine breast cancer cells were washed with PBS and lysed on ice using 1x Laemmli Sample Buffer (Biorad #161-0737) supplemented with protease (Sigma-Aldrich #P8340 ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of inflammatory cytokines were quantified using Bio-Plex Pro Human Inflammation Panel 1 37-plex (#171AL001M) magnetic bead-based assay (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Immunology 2023Quote: Monitoring of cytokine and chemokine levels was performed using Bio-Plex Pro Human Chemokine 40-plex Panel (Bio-Rad Laboratories, Hercules, California) using a Luminex 200 System ...
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bioRxiv - Immunology 2023Quote: Serum SARS-CoV-2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS-CoV-2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation for the protein lysate was performed by using SureBeads™ Protein G or A Magnetic Beads (purchased from Bio-Rad; catalog numbers1614023 and 1614013). Western blotting experiments were performed by using precast TGX 4-20% gels (purchased from Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA synthesis was performed with 1μg of RNA using PrimeScript reverse transcriptase and reverse transcribed using a PCR Instrumentation C1000 Touch(tm) Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2019Quote: ... for 5 min in 2 ml screw-cap vials with 2 g of glass beads (2mm) and 200 μl of buffer containing 1 × TE buffer and Chelex®-100 (Bio-Rad Laboratories, CA, USA). Samples were then incubated overnight at 56 °C with 10 μl of Proteinase K (20mg/ml ...
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bioRxiv - Neuroscience 2020Quote: Immunoprecipitation of transfected COS-7 cells or C2C12 myotubes transduced with shVangl2 or shScr was performed as described previously (Strochlic et al., 2012) with SureBeads™ protein G magnetic beads (BioRad, catalog no. 161-4023).
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bioRxiv - Cell Biology 2023Quote: ... was purified on a Q-Sepharose Fast-Flow column attached to an ÄKTA™ FPLC system.17 Purity (>95%) was confirmed by SDS-PAGE and staining with Coomassie Brilliant Blue G-250 (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Neuroscience 2021Quote: ... Samples were clarified by a brief centrifugation (10,000 g for 5 min at 4°C) and the supernatants were collected and diluted with 2× reducing Laemmeli buffer (Bio-Rad, Hercules, CA, USA, cat. #1610737). For preparation of cell culture lysates ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were then analysed in duplicate by means of Invitrogen’s Inflammation 20-Plex Human ProcartaPlex™ Panel (#EPX200-12185-901) and read on the Bio-Plex® 200 system (Bio-Rad, 2016). The data is expressed in pg⋅mL−1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Roughly 5 ng of genomic DNA was mixed with primers and probes for a human RPP30 copy number assay labeled with HEX (Bio-Rad, Hercules, CA) as an internal control ...
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bioRxiv - Genetics 2020Quote: ... and was quantified by using a QuantifilerR Human DNA Quantification Kit (Applied Biosystem, USA) on a Bio-Rad Real-time PCR System (Bio-Rad Laboratories, USA). The quantification process was performed per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability dye Viva Fix 498/521 (#1351115) and mouse anti-human CD138 Alexa Fluor 647 (#MCA2459A647) were obtained from Bio-Rad (Hercules, California). CD138 magnetic beads were procured from Miltenyi Biotec (Auburn ...
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bioRxiv - Cancer Biology 2023Quote: PDEC cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad). The trimmed median value was used to derive the standard curve and calculate sample concentrations.
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bioRxiv - Neuroscience 2023Quote: The reference gene used in combination with the designed human PMP22 assay was the commercially established AP3B1 (dHsaCP1000001; #10031245, Bio-Rad, Basel, Switzerland) ddPCR copy number variation assay.
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentration was assessed in cell culture supernatants 48 h post transfection by using immunoassays formatted on magnetic beads (Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y) (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol via Luminex 100 technology (Luminex ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of each of the human plasma samples from the small cohort were diluted in 17 µL of Laemmli sample buffer (Bio-Rad; Hercules, CA, USA) followed by denaturing at 70 °C for 10 min and the resulting denatured 20 µL samples were loaded onto 12% Bis-Tris PAGE gel (GenScript ...
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bioRxiv - Microbiology 2020Quote: ... Levels of the following 27 cytokines were analyzed using a BioPlex Pro™ Human Cytokine 27-plex Assay kit (#M500KCAF0Y, Bio-Rad, Hercules, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... utilizing the bead-based immunoassay approach Bio-Plex Pro™ Human Cytokine 40-plex Assay (Cat# 171AK99MR2) on a Bio-plex 200 system (Cat# 171000201) (Bio-Rad Laboratories, Hercules, CA) and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30 ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... in oral swabs of positive and negative subjects for SARS-CoV-2 using magnetic bead-based multiplex immunoassays (Bio-Plex Pro™ human cytokine 27-plex panel, Bio-Rad Laboratories, Milan, Italy) according to the pre-optimized protocol 15 ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: Culture medium of HUVECs were collected and analyzed with the Bio-Plex Pro™ Human Cytokine 27-plex Panel (Bio-Rad Laboratories, Hercules, CA, USA) in accordance with the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Luminex analyses were performed using the Bio-Plex ProTM Human Cytokine 27-Plex Assay as well as the Bio-Plex Pro™ TGF-β Assay (both Bio-Rad Laboratories, Hercules, USA) according to the manufacturer’s protocols on the Bio-Plex® Reader 200 by using the Bio-Plex Manager™ software.
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bioRxiv - Cell Biology 2021Quote: Secreted IL-6 and TNFα cytokines were measured in an amnion and choriodecidua-conditioned medium using a Bio-Plex Pro™ Human Cytokine 27-plex Assay (Bio-Rad, Marnes-la-Coquette, France) as recommended by the manufacturer along with Luminex technology ...
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bioRxiv - Immunology 2022Quote: ... The levels of soluble immune mediators were measured in TA samples using a high-throughput microbeads array (Bio-Plex Pro™ Human Cytokine 27-plex Assay, Bio-Rad Laboratories, Hercules, CA, USA), following the manufacturer’s instructions ...