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Citations for Bio-Rad :
501 - 550 of 763 citations for 6 THIOPHEN 3 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... We used 1 μL diluted cDNA (1:3 in ddH2O) and iTaq Universal SYBR Green Supermix (Bio-rad) in the Bio-rad CFX96 real-time PCR detection system for qPCR experiments ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were washed 3 times in TBS-T and incubated for 45 minutes with secondary antibody (Bio-Rad: Goat Anti-rabbit #1706515 or Goat-anti mouse #1706516) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was mixed with appropriate primers (Supplementary Table 3) and SYBR green master mix (BioRad, catalog no. 1708882) and run on a Thermocycler T100 (BioRad) ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were incubated for 3 days at 30 °C and imaged in a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... The supernatant from two reactions were collected and separated by Criterion XT Tris Acetate 3-8% (Bio-rad) SDS-PAGE and visualized by silver staining.
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bioRxiv - Biophysics 2021Quote: ... and 3.6 μL ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The 2-ΔΔCt method was used for the quantitative analysis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was extracted from several colonies of a 6-week-old subculture on Coletsos culture medium using the InstaGene matrix following the manufacturer’s instructions (Bio-Rad, Marnes-la-Coquette, France). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting transcripts were treated with DNase for 20 min at room temperature and then isolated using Micro Bio-Spin™ 6 Columns (BioRad, Cat.# 732-6221). 0.75 µg of RNA transcripts were mixed with 2X formamide buffer (95% deionized formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the SL2-6 construct was subsequently size purified using a denaturing polyacrylamide gel (7M Urea, 1x TBE, hand-poured in Bio-Rad Criterion midi cassettes), loaded in 80% formamide ...
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... was run with a 1% agarose TBE gel over 24 hours (Initial switch 1 sec, final switch 25 sec, 6 volt/cm, 120° included angle, Chef III, Bio-Rad, Hercules, CA, USA) with Midrange PFG and Lambda PFG markers (N0342S ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3 times for 10 minutes each and coated with enhanced chemiluminescence (ECL; Clarity ECL, BioRad). Using X-ray film ...
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bioRxiv - Cell Biology 2020Quote: ... Cell cycle of cells (> 3 × 104) were determined by HOECHST fluorescence in single cell by ZE5 cell analyzer (BioRad) and data were processed by Flowjo.
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bioRxiv - Biophysics 2020Quote: ... the membrane was washed 3 times with PBST and incubated with an ECL Western blot Reagent (1705060, Bio-Rad), for 5 min in the dark ...
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bioRxiv - Microbiology 2022Quote: ... NS5B-FAM probe: 5’-ATGGGTTCGCATGGTCCTAATGACACAC-3’) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 μL reaction contained 500 ng of total RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel electrophoresis was performed using the BioRad Mini-PROTEAN 3 gel electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA). Proteins were transferred to nitrocellulose membranes (Amersham™ Protran™ ...
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bioRxiv - Microbiology 2019Quote: ... caspase-9 and caspase-3 was done using a CFX-96 real-time PCR system (BioRad, Hercules, CA, USA). The reaction mixture for each sample carried 2 µL of cDNA ...
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bioRxiv - Immunology 2019Quote: Samples were diluted 1 in 3 in HBSPE and run through Bio-Gel® P-30 (Bio-Rad, UK) polyacrylamide gel spin columns to minimise non-specific binding ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were subsequently washed 3 times in lysis buffer and bound complexes were eluted using 4x Lämmli sample buffer (Biorad). Samples were boiled at 95°C for 7 minutes and resolved through SDS-PAGE prior to Western blotting or analysed by quantitative Mass Spectrometry as described in the label-free LC-MS/MS analysis section ...
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bioRxiv - Biochemistry 2020Quote: ... using a DNA Engine Opticon 3 real time PCR system (MJ Research) with Opticon Monitor 3.1 analysis software (BioRad).
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bioRxiv - Cancer Biology 2020Quote: ... Alkaline phosphatase was developed with 5-bromo-4-chloro-3-indolyl phosphate and Nitroblue tetrazolium (BCIP/NBT; BioRad, Germany) as substrate ...
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bioRxiv - Cancer Biology 2019Quote: ... ScRNA-Seq libraries were prepared using SureCell WTA 3’ Library Prep Kit for the ddSEQ System (Illumina/Bio-Rad) according to manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then transferred semi-dry for 7 min at 1.3 mV using Trans-Blot Turbo 0.2 μm nitrocellulose transfer packs and the Trans-Blot Turbo transfer system (BioRad). Blots were blocked with Odyssey blocking buffer (LI-COR ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed 3 times washing in TBS+0.05%Tween-20 and developed by enhanced chemiluminescence (Bio-Rad) with Image Quant™ LAS 4000.
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bioRxiv - Cell Biology 2022Quote: ... Samples were subjected to SDS-PAGE by resolving on a 3-8% CriterionTM XT Tris-Acetate gel (Bio-Rad) for immunoblotting.
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated using a gradient SDS PAGE gel (8-12%) and a Mini-Protean 3 Cell (Bio-Rad) at 85 V ...
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bioRxiv - Developmental Biology 2022Quote: ... 1970) and the equivalent of 0.5 - 3 oocytes/embryos were electrophoresed on SDS-PAGE TGX AnyKD Ready Gels (BioRad) or large homemade gels and transferred to nitrocellulose (Power Blotter ...
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bioRxiv - Bioengineering 2021Quote: ... All electrochemical measurements were done in a 3 electrode configuration with a graphite rod counter electrode (BioRad Laboratories, Inc.) and a Ag/AgCl reference electrode (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... Transcriptionally active Q0.3 fraction (0.32–0.4 M) were pooled and applied directly to hydroxyapatite (HAP) type II ceramic resin (Bio-Rad), washed first at 0.38 M ...
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bioRxiv - Cell Biology 2022Quote: ... All reactions were performed in 3 technical replicates using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Data was analysed according to the 2-ΔΔCT method65.
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bioRxiv - Microbiology 2019Quote: ... and 3 mg Bromophenol Blue) were separated by SDS-PAGE using 4-20% gradient polyacrylamide gels (Bio-Rad Laboratories) at 10 mAmps for 16 h ...
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bioRxiv - Microbiology 2019Quote: ... The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG) strips (Bio-Rad 1632014) and rehydrated overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated at the adequate temperature during 2-3 days and photographed with the Chemiluminiscent Imager (Bio-Rad). For methyl methanesulfonate (MMS ...
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bioRxiv - Microbiology 2021Quote: ... 3 μg of total RNAs of each sample were resolved on 10% Criterion TBE-urea polyacrylamide gel (Bio-Rad), and processed with the standard procedure described previously (63) ...
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bioRxiv - Biochemistry 2022Quote: Proteins for native mass spectrometry were held on ice and buffer exchanged into 75 µl of 50 mM ammonium acetate pH 7.5 by 3 rounds of gel filtration using BioGel P6 (Biorad) spin columns according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... followed by 3 min at room temperature using a thermocycler (MJ Mini Personal Thermal Cycler PTC 1148, Bio-Rad) Samples were centrifugated at 100 000 g for 20 min at 4 °C.
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was again washed 3 times with TBST and then placed in Clarity Max Western ECL (Bio-Rad) substrate for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... All reactions were performed in 3 technical replicates using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Data was analysed according to the 2-ΔΔCT method52.
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bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... All cDNA samples (3 biological replicates) were run in SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, 1725271) in 3 technical replicates for 40 cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... After 3 washes with TBS-T signal was developed for HRP secondaries with Clarity Western ECL Substrate (Bio-Rad). CIRBP and RPA2 expression were each measured with 3 different antibodies targeting conserved epitopes (Extended Data Figure 7c ...
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bioRxiv - Biophysics 2023Quote: ... Blotting was done by standard mini-TGX protocol (2.5 A, 25 V, 3 minutes) using a Trans-blot Turbo Transfer System (BioRad). Membranes were incubated with UBQLN2 primary antibodies (1:5000 diluted ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
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bioRxiv - Biophysics 2024Quote: ... Blots were washed 3 times with TBST for 5 min and visualized using a ChemiDoc MP Imaging System (Biorad).
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bioRxiv - Microbiology 2023Quote: ... qPCR was then performed on a Quantstudio 3 using the indicated targets and with iTaq Universal SYBR Green (BioRad). RQ values were calculated using a standard curve ...
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bioRxiv - Biochemistry 2022Quote: 5 µL aliquots of the CRISPR complex solution were buffer exchanged into 100 mM ammonium acetate using Biospin P-6 gel columns (Bio-Rad Laboratories Inc., Hercules, CA) prior to native mass spectrometry ...
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bioRxiv - Biophysics 2021Quote: ... The cleaved sample was reloaded on the same column and the flowthrough was collected and applied to a Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges (Bio-rad, Hercules, CA) for desalting ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μg of total proteins were resolved on 10% polyacrylamide gels (except for 6% gels used for PERK detection) and blotted onto PVDF membranes (#1620177, Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked with 5% not-fat dry milk or bovine serum albumin (#A7906 ...