Labshake search
Citations for Bio-Rad :
501 - 550 of 2044 citations for 2 Amino 6 8 dimethyl 3 phenylquinoline hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... whole cell lysates were separated on 8% sodium dodecyl sulfate (SDS) polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (BioRad). The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100 ...
-
bioRxiv - Developmental Biology 2021Quote: E18.5 cortical protein samples were separated on 8% SDS-PAGE gels for 1hr at 120V and transferred overnight at 20V on to PVDF membranes (BioRad). Blots were probed with rabbit anti-tubulin ß3 (1:1000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The dried pellets enriched for histones were dissolved in sample buffer (8 M urea, 5% β-mercaptoethanol and 10 mM Tris-HCl (pH 7.0)) and added sample loading buffer (Biorad XT sample buffer ...
-
Transmembrane Signaling Regulates the Remodeling of the Actin Cytoskeleton: Roles of PKC and AdducinbioRxiv - Cell Biology 2021Quote: ... RT/PCR products were analyzed by 8% polyacrylamide gel and relative band intensity was quantified using Image Lab (Bio-Rad). Most of the primer sequences used were selected from the FlyPrimerBank (Hu et al. ...
-
bioRxiv - Microbiology 2020Quote: ... 15 μL of each reaction was immediately loaded onto an 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad) supplemented with 2 mM MgCl2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Each sample was done in triplicate in a total reaction volume of 10-μl containing 0.5-μl of 1:8 diluted cDNA (validated dilution with dynamic range of amplification) and 5nM primer mix using the CFX384 QPCR machine (Biorad). The list of primers used are listed in Supplementary Table 3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The total protein were separated via 8% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Bio-Rad, Cat no. #1620177) membrane using transfer buffer (25 mM Tris ...
-
bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... Samples taken from individual fractions were analysed on denaturing SDS polyacrylamide gels (8 %) stained with Coomassie Brilliant Blue (Bio-Rad). Fractions containing pure (>90 % ...
-
bioRxiv - Microbiology 2020Quote: ... brucei were separated by SDS-PAGE (8% or 10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) (48) ...
-
bioRxiv - Neuroscience 2020Quote: ... The samples were washed and then precipitated in 0.1 M glycine pH 3.5 and neutralized with 0.5 M Tris?Cl and 1.5 M NaCl pH 7.4 before SDS-PAGE electrophoresis (8%, at 100 V for 90 min) followed by transfer to a PVDF membrane (BioRad). Membranes were washed in Tris-buffered saline (TBS ...
-
bioRxiv - Bioengineering 2020Quote: ... GEMs were transferred to PCR 8-tube strips and GEM-reverse transcription was performed in a C1000 Touch Thermal Cycler (BioRad): 53 °C for 45 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR products were separated on an 8% polyacrylamide gel stained with SYBR Gold (Thermo Fischer Scientific) and revealed using a ChemiDoc MP Imaging System (Biorad). Band intensity was quantified using ImageJ package (https://imagej.nih.gov/ij/) ...
-
bioRxiv - Microbiology 2020Quote: ... Maltose-1-phosphate was separated from orthophosphate by anion exchange chromatography using Dowex 1 × 8 100-200 mesh (Bio-Rad, 28 x 1.6 cm ...
-
bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
-
bioRxiv - Cell Biology 2021Quote: Samples were run on 8-12% SDS-PAGE gel and transferred to a nitrocellulose membrane using a Trans-Blot Turbo Transfer System (BioRad). Membrane was blocked in 5% milk in TBS + 0.1% Tween blocking solution ...
-
bioRxiv - Cell Biology 2022Quote: ... protein lysates were loaded onto 8% SDS-PAGE gel and transferred to a PVDF membrane using a semi-dry transfer system (Biorad) for 10 min at 2.5 amperes ...
-
bioRxiv - Cell Biology 2022Quote: ... 10-30 μL of the supernatants (based on protein abundances) were run on an 8% SDS-PAGE gel and transferred to a nitro-cellulose membrane (Cat. No. 1620112, BioRad) using a semi-dry transfer method (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
-
bioRxiv - Neuroscience 2022Quote: ... All steps were done manually using 8-strip PCR tubes and PCR reactions were carried out on a 96-plate thermal cycler (Biorad). Libraries were pooled and sequenced on a Nextseq550 sequencer (Illumina ...
-
bioRxiv - Neuroscience 2024Quote: Samples (8–25 μg protein extract) were run on 8–10% acrylamide gel at 100 Volt in Running Buffer (1X Tris–Glycine-SDS, Biorad) together with Precision Plus Protein Dual Color Standards (Biorad) ...
-
bioRxiv - Bioengineering 2024Quote: ... Samples in 8-well tubes were heat-shocked for three minutes at 72 °C (C1000 Touch Thermal Cycler, Bio-Rad), placed on wet ice for two minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... were resolved on SDS 8-15% polyacrylamide gels and transferred onto nitrocellulose membranes using the Trans-Blot turbo transfer system (BioRad). Membranes were blotted with the primary antibodies against ADMA (Epicypher ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein samples were boiled for 8 min at 95 °C in 1X Laemmli buffer (LB) with β-Mercaptoethanol (BioRad, 1610747), resolved on 10% polyacrylamide gels or pre-cast 4-12% Bis-Tris gradient gels (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysate and dilutions were denatured in 1x Laemmli buffer at 95C for 30 seconds and resolved by SDS-PAGE on a 8-20% gradient gel (BioRad). Following trimming of the gel ...
-
Effects of menopause and high fat diet on metabolic outcomes in a mouse model of Alzheimer’s diseasebioRxiv - Neuroscience 2023Quote: ... Diabetes-associated markers were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (171F7001M; Bio-Rad, Carlsbad, California) according to the manufacturer’s instructions.
-
bioRxiv - Physiology 2024Quote: ... 15-60 μg of denatured and reduced protein was subjected to 8-12% gradient SDS-PAGE followed by transfer to low-fluorescence polyvinylidene difluoride membranes (LF PVDF, BIORAD). Membranes were blocked with LICOR Odyssey TBS Blocking Buffer (LI-COR ...
-
bioRxiv - Immunology 2024Quote: ... were combined with Pierce Lane Marker Reducing Sample Buffer (Thermo) and applied onto SDS gels (Mini-PROTEAN TGX Gels 8-16%, BioRad for 24h/48 h/72h samples and a 10% SDS gel for resistance validation) ...
-
bioRxiv - Molecular Biology 2024Quote: ... with an 8-minute incubation at 95°C using a C1000 Touch Thermal Cycler (Bio-Rad, cat. no.185-1196). The cleaved and primed RNA was reverse transcribed into first strand cDNA using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... Polyacrylamide gels consisted of a resolving phase of 8-16% (w/v) acrylamide (37.5:1 acrylamide:bisacrylamide, 2.7% crosslinker; Bio-Rad, Watford, UK), 0.375 M Tris-HCl (pH 8.8) ...
-
bioRxiv - Bioengineering 2024Quote: ... The target genes were amplified from 8 ng of gDNA template with CFX96 Touch Real-Time PCR (Bio-Rad, USA) under the following conditions ...
-
bioRxiv - Developmental Biology 2024Quote: ... Protein quantifications were determined with a Bradford assay then 30μg of protein was separated on a 8-16% or 12% pre-cast gel (Bio-Rad) using primary antibodies diluted 1:500-1:1000 and secondary antibodies (1:2500 ...
-
bioRxiv - Cell Biology 2021Quote: ... Blocking solution consisted in 3% nonfat dry milk (Blotting-Grade Blocker, BIO-RAD Laboratories ...
-
bioRxiv - Plant Biology 2021Quote: ... 3 μL cDNA template and 4 μL iQ SYBR green supermix (Bio-Rad) in a total reaction volume of 12 μL ...
-
bioRxiv - Microbiology 2021Quote: ... were resolved by 10% denaturing PAGE in Mini Protean 3 Cell (Bio-Rad). Separated RNA was then transferred to a nylon membrane (Hybond-XL ...
-
bioRxiv - Molecular Biology 2021Quote: ... with 3 uL Precision Plus Protein All Blue prestained standards (Bio-Rad, #1610373) loaded as a ladder ...
-
bioRxiv - Genomics 2022Quote: ... and 3 μl of 2x iProof HF Master Mix (BioRad, Hercules, CA, USA). The 3 μl of 2x master mix was dispensed using a Mantis Liquid Handler (Formulatrix ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3 µl DTT and 1000 µl Purezol (Biorad, USA, CA, Cat No: 7326890) were added to each falcon tube and kept on ice for 5 minutes ...
-
bioRxiv - Bioengineering 2024Quote: 3⋅108 total particles were loaded into pre-cast gels (BioRad, Cat. # 4561094). Samples were blocked in a 5% nonfat milk solution and stained for the common EV marker CD9 using a primary antibody (Cell Signaling Technologies ...
-
bioRxiv - Bioengineering 2024Quote: ... and Precision Plus Protein Dual Color Standards (1610374, Bio-Rad, 3 μL/well). All antibodies used for western blotting were diluted at a ratio of 1:1000 or 1:500 unless otherwise specified ...
-
bioRxiv - Cancer Biology 2024Quote: ... and Bio-Plex Pro™ Mouse TGF-β 3-Plex assays (Bio-Rad) were utilized to profile the concentrations of mouse cytokines and chemokines in the cell culture supernatants ...
-
bioRxiv - Microbiology 2021Quote: 2 μl of serially diluted cDNA samples was mixed with 10 μl of 2× ddPCR supermix for probes (Bio-Rad), 1 μl of 20× WT primers/probe ...
-
bioRxiv - Plant Biology 2021Quote: ... At least 4-6 technical replicate RT-qPCR reactions were performed using iTAQ with ROX and SYBR (BioRad), and 20μL reactions were prepared as per the manufacturer recommendations ...
-
bioRxiv - Biochemistry 2022Quote: ... and 0.032 % (w/v) SM was applied to a centrifugal device (Micro Bio-Spin 6, Bio-Rad, Hercules) to exchange the buffer to a MS buffer containing 200 mM ammonium acetate (pH 7.4) ...
-
bioRxiv - Biophysics 2022Quote: ... and 1 mM EDTA in 99.9% deuterium oxide using a spin desalting column (Micro Bio-Spin 6, BioRad). 15N-HSQC spectra were measured every 40 min for 106 hours at 25 °C on Bruker 600 MHz NMR spectrometer ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA was purified by loading quenched samples onto Micro Bio-spin Columns with Bio-Gel P-6 (BioRad), followed by ethanol precipitation ...
-
bioRxiv - Developmental Biology 2020Quote: ... followed by 6 washes in 100 µL of PBST in Bio-Spin columns (catalog number 7326204, Bio-Rad). The bound immunocomplexes were eluted via 0.1 M glycine ...
-
bioRxiv - Neuroscience 2022Quote: ... then washed 6 x 5 minutes with TBS-T before developing with Clarity Western ECL Substrate (Bio-rad).
-
bioRxiv - Biochemistry 2022Quote: ... solutions containing purified CytbX were buffer-exchanged into 0.2 M ammonium acetate (pH 8.0) containing 2x CMC of Lauryldimethylamine oxide (LDAO) using Biospin 6 columns (Biorad). 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher ...
-
bioRxiv - Biophysics 2022Quote: ... then 6 L of RO water with Ca2+ chelating beads (BIO-RAD Chelex 100 Resin Cat#142-1253), and finally 6 L of RO water alone ...