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Citations for Bio-Rad :
5101 - 5150 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... After transferring the proteins to 0.45 µM nitrocellulose membranes (Biorad, Hercules, CA, USA), these were incubated for 1 hour in BSA 5% in PBS-Tween20 0.05% and then overnight at 4ºC with primary antibodies at 1:1,000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred onto a PVDF membrane in the Turbo Transblot (Bio-Rad) using the High Molecular weight program (10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred on 0.2 µm polyvinylidene fluoride (PVDF) membranes (1704157, Bio-Rad). Unspecific binding was blocked with 5% non-fat dry milk or bovine serum albumin (BSA A9647 ...
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bioRxiv - Molecular Biology 2024Quote: ... we determined protein concentration using a Bradford assay kit (Bio-Rad, Munich, Germany) with bovine serum albumin dilutions as standards ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE on a 4-15% gradient gel (BioRad) and transferred to PVDF membrane (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein lysate samples were run in a 15% SDS-PAGE gel (Biorad), followed by protein transfer (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Protein concentration in soluble lysate fraction was measured by Bradford assay (Bio-Rad Protein Assay Dye Reagent Concentrate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein bands were developed by Clarity™ Western ECL Substrate (Bio-Rad, USA) for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Collected the clear supernatant and quantified using a Bradford protein assay (Bio-Rad), aliquoted ...
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bioRxiv - Plant Biology 2021Quote: ... Leaf disks were ground to a fine powder evenly with a grinding tool and 120 μl extraction buffer added (30 μl Laemmeli sample buffer 4x [Bio-Rad], 24 μl DTT 1 M, 66 μl dH2O). Tubes were vigorously vortexed for 90 seconds ...
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bioRxiv - Plant Biology 2020Quote: The protein content in the enzyme extracts was determined by using Bradford assay (BioRad) (Bradford ...
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bioRxiv - Biophysics 2021Quote: ... Conjugated proteins were detected by the ChemiDoc™ Touch Imaging System (Bio-Rad, USA) and quantified by ImageLab software (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... pulled-down proteins were eluted from the beads using 2x laemmli buffer (Bio-Rad) at 95 °C for 5 min incubation in heat block at 1000 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... This was further followed by wet transfer of proteins onto nitrocellulose membrane (1620112, BioRad). After transfer ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:2000)] proteins were detected using ClarityTM Western ECL Substrate (Bio-Rad. #170-5060). All western blots were repeated at least 3 times.
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bioRxiv - Cancer Biology 2021Quote: ... Blots were scanned and protein expression quantified in a ChemiDoc XRS+ equipment (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Protein 1ug/ml were immobilized onto NC membranes (0.22-m pore size; Bio-Rad). The membrane kept for drying for 5-10 min ...
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bioRxiv - Cell Biology 2020Quote: ... and their concentrations were measured with a RC/DC protein assay kit (Bio-Rad). Supernatant (1 ml at ~4-5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... and bound protein was assessed by SDS-PAGE stained with QC Colloidal Coomassie (BioRad). Lipid recovery was normalized to the input samples by measuring relative DiR fluorescence on an Odyssey Imager (Li-COR).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose using a Trans-Blot Turbo Transfer System (Bio-Rad). Primary antibodies used were mouse anti-alpha tubulin (DM1A ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were transferred to nitrocellulose membranes using wet-based transfer system (Bio-Rad). Membranes were incubated overnight with the indicated primary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to nitrocellulose using a Trans-Blot Turbo system (Bio-Rad Laboratories). Blots were probed with primary antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the protein concentrations in clarified lysates were estimated using the Bradford assay (Bio-Rad). The culture supernatants were collected and filtered through a 0.4 μm membrane filter ...
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bioRxiv - Cell Biology 2020Quote: ... 10-50ug of total protein sample was mixed with 4x Laemmli buffer (Bio-Rad) and heated at 98 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... The protein content was determined with a chemiluminescence (ECL) assay kit (Bio-Rad, 1705060).
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration of the clear supernatant was determined by Bradford (BioRad, C500-0205) protein assay ...
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bioRxiv - Immunology 2021Quote: ... Proteins were separated by SDS/PAGE on precast 4-15% acrylamide gels (Bio-rad) and transferred to TransBlot® Turbo™ Midi-size PVDF membranes (Bio- rad) ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of proteins were separated on 4-12% SDS-polyacrylamide gel (Bio-Rad), transferred onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... The amount of total protein was measured with Lowry colorimetric assay (DC, Bio-Rad) for fat bodies and Bradford standard assay (Bio-Rad 500-0006 ...
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bioRxiv - Cancer Biology 2019Quote: ... 15-20μg denatured protein from each sample was run in XT sample buffer (BioRad) with 0.25% β-mercaptoethanol on 10% or 12% SDS-PAGE Bis-Tris gels (pre-cast BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Prior to loading on a 4-20% mini-PROTEAN TGX precast protein gel (BioRad), samples were boiled for 10 min at 99 °C in a thermoblock while shaking at 1,000 rpm ...
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bioRxiv - Developmental Biology 2021Quote: Proteins from each sample were resolved using a precast 8-16% gradient gel (Biorad), and transferred to a PVDF membrane following manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: 100 µl of magnetic beads (SureBeads™ Protein A Magnetic Beads, Bio-Rad, USA) were washed in 1 ml of 1×PBS for 5 times ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Proteins were separated by SDS-PAGE using Mini-PROTEAN TGC gels (BioRad #456–1084) or Mini-Protean TGX Stain-Free (BioRad #456-8084) ...
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bioRxiv - Physiology 2022Quote: ... alongside two protein markers (Precision plus all blue and dual color standards, Bio-Rad), three human skeletal muscle control samples and a four-point standard curve (skeletal muscle samples of increasing volume) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to nitrocellulose using a Trans-Blot Turbo Transfer System (Bio-Rad). Primary anti-bodies were diluted in 4 % BSA in PBS and used as follows ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were electrotransferred onto a polyvinylidene difluoride membrane (PVDF, Bio-Rad, cat. #1620177). Membranes were blocked for 5 min in Tris-buffered saline containing 0.1% Tween-20 (TBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were visualized by ECL plus a Molecular Imager (ChemiDoc XRS+, BIO-RAD) with Image Lab software (BIO-RAD).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to an absolute methanol-activated (10s) PVDF membrane (Bio-Rad 1620177) at 100 V for 1 hr in 4°C pre-chilled 1X Tris-Glycine buffer (Bio-Rad 161-0734) ...
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bioRxiv - Molecular Biology 2020Quote: ... the obtained cell extracts were pre-cleared with uncrosslinked Affiprep Protein-A beads (BioRad) for at least 1 hour at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins (5 μg) were separated on a 4–15 % polyacrylamide gradient gel (Bio-Rad) and then transferred to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 µg of protein with equal volume of 4X Laemmli sample buffer (Bio-Rad) was denatured at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase’s signals were normalized to total protein amount determined with RC-DC kit (BioRad).
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibodies were used to detect the proteins by enhanced chemiluminescence (Bio-Rad #1705061). Quantification of protein levels was determined using ImageJ software and normalized to loading control (Tubulin) ...
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bioRxiv - Cell Biology 2022Quote: ... The total protein amount of the lysates was determined by Bradford assay (Bio-Rad). 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare ...
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bioRxiv - Genomics 2020Quote: ... Proteins in the gel were electrophoretically transferred to polyvinylidene difluoride membranes (Bio-Rad, CA) and subjected to immunoblotting with the antibodies for phosphorylated Erk-1/2 (Thr202/Tyr 204 ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 µg of protein was loaded into precast 4-15% gradient gels (Bio-Rad) and run at 180 V for 60 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were visualized using the ChemiDoc XRS system with Image Lab software (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... Protein band intensities were quantified via densitometry analysis with Quantity One software (Bio-Rad). Raw intensity values were normalized to the measurement of a β-tubulin loading control and expressed as the n-fold change to an appropriate control genotype ...
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bioRxiv - Microbiology 2019Quote: Boiled protein samples were loaded onto either 4-20% gradient SDS-PAGE gels (BioRad), 12% or 15% SDS-PAGE gels ...