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Citations for Bio-Rad :
5001 - 5050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the membrane was soaked with Clarity Western Enhanced Chemiluminescence (ECL) Substrate (Bio-Rad; Cat. No. 170-5060), and Western blot bands were visualized using the GelDoc Go Imaging System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... The reaction was performed in a thermal cycler (CFX Touch Real-Time PCR Detection System, Bio-Rad) with the heating scheme as follows ...
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bioRxiv - Microbiology 2024Quote: ... after which proteins were purified using poly-prep chromatography columns (Bio-Rad). Bivalent Fc nanobodies were eluted with 0,1 M citric acid (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were electrophoresed and transferred to a 0.45-μm nitrocellulose membrane (Bio-Rad). Membranes were blocked with tris-buffered saline Tween (TBST ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time quantitative PCR was carried out on cDNA samples using SsoAdvanced Universal SYBR Green Supermix (BioRad). The primer sequences were as follows ...
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bioRxiv - Cell Biology 2024Quote: iScript Advanced cDNA Synthesis Kit for PCR (BioRad) was used for Reverse Transcription (RT ...
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bioRxiv - Cell Biology 2024Quote: ... Image Lab 6.1 (Bio-Rad Laboratories) and AzureSpot Pro (Azure Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... antibody binding was detected with either the Clarity or Clarity Max Western ECL substrate (Bio-Rad) and ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Sample was separated on pre-cast 4-20% gradient gels (Bio-Rad) which were transferred onto nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... which were transferred onto nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in SEA BLOCK buffer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred to a nitrocellulose membrane (Bio-Rad), and then incubated with the indicated antibodies ...
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bioRxiv - Cell Biology 2024Quote: cDNA was synthesized from 500 ng RNA using the iScript™-cDNA Kit (Bio-Rad Laboratories, Hercules, CA, USA). Real-time quantitative PCR was carried out using mRNA-specific primers (Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... Horseradish peroxidase-conjugated secondary antibodies (1:3000) were purchased from BioRad.
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to PVDF membrane (Bio-Rad; 1620177). The membranes were blocked using 5% skim milk in TBST buffer (20mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... on a CFX qPCR machine (Bio-Rad). qRT-PCR data were analyzed using CFX Manager 3.1 (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (1706515) was purchased from BioRad. Trizol (15596018) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were determined using BSA protein assay (Bio-Rad). SDS-PAGE electrophoresis and Western blotting were conducted as previously described.25 The primary antibodies used were ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse-transcribed into cDNA using an iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA). qRT-PCR was performed using the PerfectStart Green qPCR SuperMix kit (TransGen Biotech ...
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bioRxiv - Cancer Biology 2024Quote: ... and 70°C to denature the enzyme using DNA Engine Dyad thermal cycler (Bio-Rad Laboratories). The synthesized cDNA was stored at -20°C for further use.
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bioRxiv - Cancer Biology 2024Quote: ... The levels of mRNA were analyzed by the Bio-Rad CFX96 real-time PCR system (Bio-Rad, Hercules, CA, USA). GAPDH was used as an endogenous control for mRNA qualification and the 2-ΔΔCt method was applied to calculate the relative expression ...
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bioRxiv - Cancer Biology 2024Quote: ... Western blotting materials were from Bio-Rad and western bright ECL HRP substrate from Advansta (USA) ...
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bioRxiv - Cancer Biology 2024Quote: Reverse transcription quantitative real-time PCR (RT-qPCR) reactions were performed in 96-well plates (BioRad CFX platform) by using SyBr green in a total reaction volume of 10μl per well ...
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bioRxiv - Cancer Biology 2024Quote: ... The blots were blocked using 5% non-fat dry milk (Catalog No. 1706404, BioRad) and/or 5% BSA and incubated with primary antibodies at 4□C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant used following protein quantification (Bio-Rad #5000111).
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bioRxiv - Cell Biology 2024Quote: ... HRP conjugated secondary antibodies against appropriate species were used and signal developed by using Clarity Western ECL substrate (Biorad, USA). For co-immunoprecipitation ...
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bioRxiv - Cell Biology 2024Quote: ... Images were acquired and analyzed using a chemidoc system from Bio-Rad.
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bioRxiv - Cell Biology 2024Quote: ... Secondary horseradish peroxidase-coupled antibodies (1:3000, Pierce, α-mouse #1858413, α-rabbit #1858415) were applied prior to ECL-based chemiluminescence (Biorad #1705061 and ThermoFisher #34094) detected using a Fusion Fx instrument (Vilber Lourmat) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Cell Biology 2024Quote: ... resolved onto 10% SDS-PAGE and Western transferred to PVDF membrane (Bio-Rad # 1620177) to probe for proteins of interest ...
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bioRxiv - Cell Biology 2024Quote: ... or DC method (Bio-Rad #5000111) were used to measure the protein concentration.
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bioRxiv - Cell Biology 2024Quote: ... qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882, Bio-Rad). The relative expression values were determined by normalization to GAPDH transcript levels and calculated using the ΔΔCT method ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins samples were denatured in SDS-sample buffer for 5 min at 95°C and loaded onto Mini-Protean TGXTM gels (BioRad Laboratories) in equal amounts and subjected to electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... SsoAdvanced Universal SYBR Green Supermix (1725270, Biorad) was purchased ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed thrice and incubated with appropriate secondary antibodies conjugated with HRP (Bio-rad; 1:3,000). Bands were detected by Western-Lightning Plus Enhanced Chemiluminescence (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2024Quote: ... run on 4-20% pre-cast gels (Bio-Rad Mini-PROTEAN TGX Gels Cat# 4561095) using the BioRad Mini-PROTEAN system ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and purity of extracted protein was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006) following manufacturer’ s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... 10-30 µg of protein was resolved on Criterion TGX Precast Midi Protein Gels (Bio-Rad) then a wet transfer onto Immobilon-FL (Millipore) ...
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bioRxiv - Cancer Biology 2024Quote: ... using Image Lab software (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad #1706516), Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (Bio-Rad #1706515) ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred to nitrocellulose membranes (Bio-Rad Trans-Blot Turbo), evaluated for transfer by Ponceau S (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... brightness/contrast was adjusted either on the ChemiDoc or using Image Lab (Bio-Rad). Antibodies used and their dilutions were ...
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bioRxiv - Cancer Biology 2024Quote: ... separated by denaturing electrophoresis on Criterion TGX 4-15% gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad Trans-Blot Turbo) ...
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bioRxiv - Cancer Biology 2024Quote: ... Goat anti-Mouse IgG (H+L)-HRP conjugate (Bio-Rad, 1706516, 1:3000).
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bioRxiv - Cancer Biology 2024Quote: ... the cells were imaged using a fluorescence microscopy (ZOE™ Fluorescence Imaging, Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Goat anti-Rabbit IgG (H+L)-HRP conjugate (Bio-Rad, 1706515, 1:3000), Goat anti-Mouse IgG (H+L)-HRP conjugate (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to Immun-Blot PVDF membranes (Bio-Rad) and then blocked with 5% milk in tris-buffered saline with 0.1% Tween (TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... Analysis was performed at a wavelength of 570 nm by a microplate reader (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... 10 μl of the cell suspension was used to estimate the number of cells by an automated cell counter (BioRad TC20). Based on this ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 1×SYBR green master mix (Bio-Rad), 4µM forward and reverse primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cDNA was reverse transcribed from 500ng of RNA using iScript IV VILO cDNA synthesis kit (Bio-Rad), with reactions incubated at 25°C for 5 min ...