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Citations for Bio-Rad :
451 - 500 of 3900 citations for Who Coplanar & Mono Ortho Pcb + Pcb 170 & Pcb 180 13C12 99% 20 Ng Ml In N Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Each qPCR Real-time reaction was carried out in a total volume of 10 µL with 20 ng of cDNA using SYBR-Green PCR Master Mix (BioRad) and an iCycler IQ Real-time PCR System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... concentration was fixed at 5% while N,N-methylene-bis-acrylamide (bis, Bio-rad) varied from 0.04% to 0.1% to attain different stiffnesses of the substrates ...
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bioRxiv - Cell Biology 2023Quote: ... concentration was fixed at 5% while N,N-methylene-bis-acrylamide (bis, Bio-rad) varied from 0.04% to 0.1% to attain different stiffnesses of the substrates ...
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bioRxiv - Cell Biology 2019Quote: ... The extracted RNA was then reverse transcribed to cDNA using iScript cDNA Synthesis Kit (BioRad, 170-8891), which was then used to perform quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Genetics 2020Quote: ... prior to synthesizing cDNA using the Bio-Rad iScript Reverse Transcription Supermix kit (Bio-Rad, 170-8891). We also included controls lacking Reverse-Transcriptase to assay for the presence of genomic DNA contamination ...
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bioRxiv - Developmental Biology 2020Quote: ... blots were incubated with either horseradish peroxidase (HRP)–conjugated goat anti-mouse antibody (Bio-Rad 170-6516) or HRP–conjugated goat anti-rabbit antibody at a concentration of 1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Gene expression was quantified by RT-qPCR using iQ SYBR Green supermix (Bio-Rad, Cat.# 170-8886). The relative gene expression was calculated using the 2-ΔΔα method and normalized to GAPDH ...
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bioRxiv - Microbiology 2019Quote: ... RNA was then converted into cDNA using the iScript cDNA synthesis Kit (170-8891) from Bio-Rad. Quantitative real time PCR was performed using SsoAdvanced Universal SYBR Green Supermix (172-5271 ...
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bioRxiv - Immunology 2021Quote: Reverse transcription was performed using iScript™ Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 170-8840) with 100 ng of total RNA per 20 μL reaction ...
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bioRxiv - Cell Biology 2021Quote: ... 300ng of RNA were reverse transcribed into cDNA using iScript cDNA synthesis kit (Bio-rad, 170-8891). Real-time qPCR was then performed in duplicates using CFX-96 real time PCR device (Bio-rad) ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, #170-6515, Biorad or #12-349 ...
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bioRxiv - Molecular Biology 2020Quote: Complementary DNA (cDNA) was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad, Cat. No. 170-8891) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... HRP secondary antibodies were used as follows: 1:500 to 1:1000 anti-mouse (BioRad, 170–6516) and 1:500 to 1:1000 anti-rabbit (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ Transfer Pack (Biorad#170-4159). The nitrocellulose membrane was blocked in a buffer (5% milk in Tris-buffered saline with containing 0.01% Tween20) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Visualization of the proteins on the membrane was performed by Enhanced chemiluminescence (ECL) (Bio-Rad 170-5060) via Image Lab Software (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... Separated proteins were transferred into a PVDV membrane using a mini trans-blot cell (BioRad 170-3930) at 200V for 1h ...
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bioRxiv - Cell Biology 2019Quote: ... and then transferred onto 0.2 µm nitrocellulose membranes (Trans-Blot Turbo Transfer Pack; Bio-Rad, 170-4158). Membranes were blocked with blocking buffer (Thermo Scientific™ Pierce™ StartingBlock™ ...
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bioRxiv - Bioengineering 2019Quote: ... Transfers were performed using a semi-dry turbo transfer system onto PVDF membranes (Bio-Rad 170-4274). The membranes were blocked with 5% non-fat milk in TBS (60.55 g/L Tris Base ...
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA synthesis was carried out with the iScript cDNA Synthesis Kit (Bio-Rad, no. 170-8891SP) according to the manufacturer’s instructions with 500 ng of purified RNA per sample in 20 μl ...
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bioRxiv - Microbiology 2020Quote: ... After washing the membrane was then visualized using secondary HRP conjugated antibodies (Bio-Rad cat 170-6515) (1:30,000 ...
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bioRxiv - Microbiology 2021Quote: ... Non-specific binding to the membrane was blocked with 5% Blotting-Grade Blocker (BIO-RAD, #170-6404), followed by probing with 1:2000 dilution of monoclonal anti-flag antibody (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 ug of RNA using the iScript cDNA Synthesis Kit (BioRad #170-8891). Reverse Transcriptase Quantitative PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... The specific signals were revealed using the Clarity Western ECL Substrate from Bio-Rad (Cat. 170-5061).
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bioRxiv - Microbiology 2022Quote: ... and RNA was then converted to cDNA using iScript cDNA Synthesis Kit (Bio-Rad catalog #170-8891), with 4 μL 5x iScript reaction mix ...
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bioRxiv - Microbiology 2022Quote: ... Detection step was performed using the Enhanced chemiluminescence (ECL) system (Clarity Western ECL substrate #170-5060, Biorad) following manufacturer instruction ...
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bioRxiv - Physiology 2022Quote: ... Signals were detected using Clarity™ Western chemiluminescent substrate (cat#170-5061, Bio-Rad, Hercules, California, USA) and were imaged using a Bio-Rad ChemiDoc™ Imaging System (Hercules ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 h at room temperature in 5% skim milk (Catalog #170-6404, Bio-Rad). Primary antibody incubations were performed overnight at 4°C with antibodies diluted in TBS/0.1% Tween-20/5% BSA ...
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bioRxiv - Biochemistry 2023Quote: ... The tRNA was transferred onto nylon membrane by TRANS-Blot SD (Cat#170-3940, Bio-Rad, USA) at 650 mA for 30 min and the membrane was washed briefly in 5 × SSC (0.75 M sodium chloride ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes probed with HRP conjugated antibodies were developed using Clarity™ Western ECL Substrate (BioRad, 170-5061). Membranes were imaged using BioRad ChemiDoc™ MP Imaging System.
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized with 0.5 μg of RNA using the cDNA Synthesis Kit (Bio-Rad 170-8891) using random hexamers to generate cDNA from total RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were separated on a 15% SDS-PAGE gel and visualized with ECL (Bio-Rad, #170-5061).
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bioRxiv - Cell Biology 2023Quote: ... 300ng of RNA were reverse transcribed into cDNA using iScript cDNA synthesis kit (Bio-rad, 170-8891). Real-time qPCR was then performed in duplicates using CFX-96 real time PCR device (Bio-rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary antibody against rabbit IgG (Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (170-6515, Bio-Rad)) was used at a dilution of 1;25,000 for detection of Actin and Venus ...
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bioRxiv - Cancer Biology 2024Quote: ... the prepared samples were spotted on the membrane using a Dot Blot apparatus (Bio-Rad, 170-6545) and UV-crosslinked for 3 min at 1200 J/m2 ...
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bioRxiv - Genetics 2023Quote: HRP secondary antibodies were used as follows: 1:500 to 1:1000 anti-mouse (BioRad, 170-6516), 1:500 to 1:1000 anti-rabbit (BioRad ...
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bioRxiv - Immunology 2024Quote: ... It was then reverse transcribed using iScript cDNA synthesis kit (cat #170-8891) purchased from Bio-Rad. Gene expression analysis for the cardiac graft was performed by qRT PCR using Fast SYBR Green mastermix on a Applied Biosystems step one plus real time PCR system ...
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bioRxiv - Physiology 2020Quote: ... A total of 1000 ng of total RNA was used to prepare cDNA in a 20 µL reaction with Script Reverse Transcription Supermix (Bio-Rad). qPCR was performed on technical duplicates with cDNA diluted 30x and SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Amplification of cDNA (21 ng) was performed in a total volume of 20 μL of iTaq Universal Probes Supermix (Bio-Rad), using Real-Time QuantStudio7 Flex (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... Technical replicates of 20 ng were used to measure Ct on a BioRad CFX96 thermocycler with iTaq UniverSYBR Green SMX (BioRad 1725125). Relative expression was calculated against the mean of two housekeeping genes ...
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bioRxiv - Zoology 2022Quote: ... RNA libraries were diluted to 20 ng μL−1 and reverse transcription was performed with an iScript cDNA synthesis kit (Bio-Rad). Primers for five genes—Clock ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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bioRxiv - Bioengineering 2023Quote: ... 35 µL N,N-methylenebisacrylamide crosslinker (2% w/v bis-acrylamide Solution, 1610142, Bio-Rad), 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm ...
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bioRxiv - Bioengineering 2024Quote: ... 35 µL N,N-methylenebisacrylamide crosslinker (2% w/v bis-acrylamide Solution, 1610142, Bio-Rad), 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm ...
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bioRxiv - Genetics 2022Quote: ... Approximately 43µl of the newly formed droplet solution were transferred into a ddPCR 96-well plate and then sealed at 180°C for 5 seconds in the PX1 PCR Plate Sealer (BioRad). PCR was carried out with the following conditions ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run for 5 min at 130 V followed by 55min min at 180 V (PowerPac Basic Power Supply (Biorad)) ...
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bioRxiv - Plant Biology 2019Quote: ... and lµg of eluted RNA was used for complementary DNA (cDNA) synthesis using iScript (Bio-Rad, 170-8891). Samples were analyzed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized using the iScript cDNA Synthesis Kit according to manufacturer’s protocol (170-8891; Bio-Rad Laboratories). Quantitative PCR was performed with iQ SYBR® Green Supermix (170-8880 ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR (Real-Time quantitative PCR) was performed using iQ SYBR Green Supermix solution (Bio-Rad, #170-8880). The CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:15000 in blocking buffer) followed by a goat anti-rabbit-HRP conjugate (170-6515, Bio-Rad, Hercules ...