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Citations for Bio-Rad :
451 - 500 of 1532 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... with 200 nM of each gene-specific forward and reverse primer (Supplemental Table S1) mixed with SYBR® Green supermixes (Bio-Rad, USA) according to the manufacturer’s instructions on a Thermal Cycler CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... immunoprecipitated DNA samples were analyzed using primers described in Table S1 in technical triplicate using iTaq Universal SybrGreen reagents (BioRad, Hercules, Ca, USA) in a RealPlex Instrument (Eppendorf ...
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bioRxiv - Plant Biology 2023Quote: Real-time quantitative PCR was performed using gene-specific primers (Table S1) for selected genes on CFX96 Touch™ Real-Time PCR Detection System (Biorad, California, USA). The amplification conditions were ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was diluted 10 fold in nuclease free water and reactions were performed in a Bio-Rad CFX96 system using gene specific primers (Table S1) and Bio-Rad SYBR green master mix (Bio-Rad, Hercules, USA). Each sample was run in triplicate ...
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bioRxiv - Microbiology 2024Quote: ... blaOXA-48) and the intI1 gene by PCR methods 20-23 (Supplementary tables S1 and S2) in a Thermocycler (BIO-RAD T100™) using specific primer sets (Supplementary table S2) ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... TAG levels were normalized to protein amounts in each homogenate using a Bradford assay (Bio-Rad), and data were analyzed using a student’s t test ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was exposed to a peroxidase substrate (Clarity Western ECL Substrate, Bio-Rad) for 5 min at 23°C ...
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bioRxiv - Microbiology 2019Quote: ... Horse radish peroxidase-conjugated secondary antibodies were detected using Clarity Western ECL substrate (BioRad). Blots were visualized using the BioRad ChemiDoc MP imaging system.
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bioRxiv - Developmental Biology 2023Quote: ... for detecting peroxidase-conjugated secondary antibodies and analyzed with an ChemiDoc Touch (Bio-rad).
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bioRxiv - Cancer Biology 2024Quote: ... Peroxidase chemiluminescence reaction was enhanced with Clarity™Western ECL Blotting Substrates (Bio-Rad) and detected through the Chemidoc detection system (Bio-Rad) ...
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bioRxiv - Biophysics 2023Quote: ... purified RBD-Fc proteins were concentrated using 10,000 MWCO Amicon centrifugation columns (BioRad, California, USA) and stored at 4°C before use ...
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bioRxiv - Plant Biology 2020Quote: Relative expression of CgCOM1 transgene in chilli- and tomato-CgCOM1i transgenic lines was analyzed by RT-qPCR using CgCOM1 gene specific primers (Supplementary Table S1) and SSoFast™ EvaGreen® supermix (Bio-Rad, USA) on CFX96 Touch™ Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: Protein overexpression and purification were analysed via 10% SDS-PAGE and imaged via ChemiDoc™ MP Imaging System (Bio-Rad; Fig. S1 and S4). Immunoblotting was performed to confirm purification results with primary anti-6xHis polyclonal mouse antibody (Beyotime® ...
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bioRxiv - Developmental Biology 2022Quote: ... HRMA is a powerful tool to screen indel mutations or single nucleotide polymorphism in the samples and it was performed on the genomic DNA with specific primers listed in Table S1 using the CFX96 Real-Time PCR Detection System and the Precision Melt Analysis software (Bio-Rad Laboratories, Hercules, CA). HMA was performed with PAGE to verify the genotyping results of HRMA ...
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bioRxiv - Cell Biology 2021Quote: ... MYC tag (CST #2278) Secondary antibodies: Goat Anti Rabbit IgG (H+L) HRP Conjugate (BioRad #172-1019); Goat Anti Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: Mouse monoclonal anti-Viral V5-TAG antibody (1:5000; clone SV5-Pk1) was purchased from Bio-Rad/AbD Serotec ...
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bioRxiv - Biochemistry 2023Quote: ... The purity and sequence of the tag-cleaved SLO protein were confirmed by SDS-PAGE (Bio-Rad) and bottom-up MS (BU-MS) ...
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bioRxiv - Neuroscience 2022Quote: ... Peroxidase activity was detected using chemiluminescence regent (Clarity Western ECL substrate, Bio-Rad Laboratories, Inc.) and visualized by an image analyzer using Image Lab™ Software (Gel Doc™ XR+ ...
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bioRxiv - Microbiology 2021Quote: ... samples were developed with 50µl/well of Single-Component TMB Peroxidase EIA Substrate Kit (BioRad) for 5-10 min ...
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bioRxiv - Microbiology 2023Quote: ... incubated for one hour with horse radish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse: Biorad, 172- 1011 ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR was conducted with primers indicated in Table S1 and ChamQ Universal SYBR® qPCR Master Mix (Vazyme, China) on a Bio-Rad CFX384 qPCR cycler (Bio-Rad Laboratories, Shanghai, China).
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bioRxiv - Microbiology 2019Quote: ... and BB2-epitope tag (1:4,000) followed by HRP-conjugated goat anti-rabbit antibody (1:4,000; Bio-Rad). Membrane blocking ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Biochemistry 2022Quote: Supernatants from Wuhan SARS CoV-2 infected cells prepared as described above or uninfected cells and commercial spike were lysed with 2x Laemmli Sample Buffer (#1610737, Bio-Rad, Hercules, CA, USA) with DTT (#EU0006-B ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2 M 2-Mercaptoethanol (Bio-Rad), and boiled for 3 min ...
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bioRxiv - Cancer Biology 2019Quote: ... a bottom layer containing RPMI 1640 medium (10% CS-FCS) and 0.75% low melting agar (Bio-Rad) was overlain with half the volume of RPMI 1640 medium (10% FCS ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were immunoprecipitated again via the HA-tag and analyzed by 4-15% non-reducing gradient SDS-PAGE (BioRad) and processed as before.
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bioRxiv - Cell Biology 2021Quote: ... followed by a one hour incubation in the appropriate secondary antibodies coupled to peroxidase (1:1000, Biorad). Proteins were detected by enhanced chemiluminescence detection (ECL ...
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bioRxiv - Biophysics 2020Quote: ... combined with the secondary anti-horse radish peroxidase (HRP) anti-rabbit antibody (Bio-Rad, Hercules, CA, USA), and an anti-biotin monoclonal antibody (Hsc70 ...
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bioRxiv - Immunology 2023Quote: ... Detection was carried out with a goat anti-rabbit horse radish peroxidase (HRP) conjugated secondary antibody (BioRad), visualized with the enhanced chemiluminescent SuperSignal reagent (Pierce) ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were goat anti-mouse horse radish peroxidase (HRP)-conjugated (1706516, Bio-Rad, RRID: AB_11125547) at 1:10,000 and goat anti-rabbit HRP-conjugated (1721019 ...
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bioRxiv - Neuroscience 2024Quote: ... The peroxidase reaction for AffiniPure antibodies was developed with Clarity™ Western ECL substrate (Bio-Rad, 1705061). Imaging was performed using either an Odyssey Fc imaging system (Li-Cor ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins and peptides bearing C-terminal 6xHis tags were detected with mouse anti-6xHis antibody clone AD1.1.10 (BioRad; Cat # MCA1396GA) while detection of glycosylated CRM197-FtO-PS was with anti-F ...
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bioRxiv - Biochemistry 2021Quote: ... with or without a C-terminal FLAG™-tag followed by a foldon trimerization domain and an octahistidine tag was heated to 95 °C in XT sample buffer (Biorad) for 5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... We utilized colorimetric detection to visualize the MBP-Vpu band(s) on the membrane after WB transfer: Primary mouse anti-histidine tag antibody (BioRad) and secondary goat anti-mouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... TAG content was normalized to total protein determined using a standard Bradford Protein assay (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Immunology 2024Quote: ... rabbit anti-FLAG-HRP (clone D6W5B, CellSignalingTechnology #86861S, RRID:AB_2800094) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P, RRID:AB_609796) detection Abs were added for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... We utilized colorimetric detection to visualize the apoAI-EfpA band(s) on the membrane after WB transfer: The primary mouse antihistidine tag antibody (BioRad) and secondary goat antimouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Biochemistry 2020Quote: ... Biobeads SM-2 (Biorad; 2 g; prewashed in nanodisc buffer) were added ...
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bioRxiv - Genomics 2020Quote: ... 2% CleanCut (BioRad) agarose was melted at 70°C then cooled to 50°C ...
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bioRxiv - Microbiology 2021Quote: ... blots were re-probed with rabbit anti-mouse IgG peroxidase-conjugated antibodies (Bio-Rad / AbD Serotec; Hercules, CA) diluted 1:40,000 in PBS-T.3 ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 were incubated overnight at 4°C and revealed using peroxidase-conjugated antibodies and an ECL kit (BioRad). Images were captured using ChemiDocTM XRS Imaging system (Biorad).
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bioRxiv - Plant Biology 2023Quote: ... the membrane was incubated with 1:10,000 dilution of anti-mousehorseradish peroxidase (anti-mouse-HRP) antibody (Bio-Rad) at room temperature for 1 h and washed again three times in TBST ...
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bioRxiv - Microbiology 2023Quote: ... Anti-mouse horse radish peroxidase-conjugate (170-5947) and anti-GAPDH (clone 6C5, MCA4739) were purchased from BioRad. Anti-β-actin (clone C-4 ...
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bioRxiv - Microbiology 2021Quote: ... with the C-terminal fluorescent tag and an empty vector containing only fluorescent tag was bombarded separately on onion peels containing MS plates using the PDS1000/He system at 1100 psi (BioRad, USA). After 36 hrs ...
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bioRxiv - Microbiology 2021Quote: ... were treated with 20% blocking buffer (Li-Cor Biosciences, Lincoln, US) followed by incubation with anti-His-tag mouse primary antibody (dilution1/5000, Bio-Rad) and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000 ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...