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Citations for Bio-Rad :
451 - 500 of 1252 citations for PLGF 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Detergent was removed by adding 150mg of BioBeads SM-2 (Bio-Rad) for 3h ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT) using a Bio-Spin P-30 column (Bio-Rad). The concentration of the recovered protein complex was estimated based on its absorbance at 280 nM ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: ... Non-ionic detergents were removed using Bio-Beads SM-2 (Bio-Rad).
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bioRxiv - Biophysics 2020Quote: ... In the morning ∼100 uL more Bio-Beads SM-2 (Bio-Rad) were added and 2x molar excess of FabDH4 ...
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bioRxiv - Neuroscience 2021Quote: ... which was diluted and denatured in 2× SDS sample buffer (Bio-Rad). Samples were subject to SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... 2 μl of anti-rabbit T lymphocytes fluorescently labeled with FITC (BioRad), and 2 μl of anti-rabbit IgM fluorescently labeled with FITC (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μl of anti-rabbit IgM fluorescently labeled with FITC (BioRad), and then incubated for 15 min at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... 2% paraformaldehyde (PFA) in 1X phosphate buffered saline (PBS; BioRad, 161-0780) at 4°C ...
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bioRxiv - Genomics 2019Quote: ... Cells equivalent to 600ng DNA were embedded in 2% Agarose (Bio-rad) and solidified in 4°Cfor 45 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.6 μL H2O and 11 μL 2× ddPCR Supermix (Bio-Rad #1863024). 20 μL of ddPCR mixes were used to generate droplets in a QX200 Droplet Generator (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... followed by removal of detergent through absorption by BioBeads SM-2 (BioRad) to force the formation of proteoliposomes ...
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bioRxiv - Cell Biology 2020Quote: ... actin (see Table 2 for sequences) and iTaq Mastermix (BioRad: 172-5171) in a BioRad CFX Connect real time system ...
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bioRxiv - Developmental Biology 2021Quote: ... and dried for 2 h in a GelAir drying system (Bio-Rad). The dried gel in cellophane was exposed for at least 12 h to a BAS-IP MS phosphor screen (GE 28956475) ...
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bioRxiv - Genomics 2021Quote: ... 2 μl of Reverse Transcriptase (Bio-Rad, final concentration 20 U/μl), 1 μl of DTT (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... for 2-5 min and scanned with GS-800 densitometer (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Lysis was performed by directly adding 2× Laemmli sample buffer (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2022Quote: ... samples were mixed 1:1 with 2 x native sample buffer (BioRad) and loaded on a 4-20% precast protein gel (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... and cleaned with the ReadyPrep 2-D cleanup kit (1632130, Bio-Rad). The pellet obtained was dissolved in 10 μL of 20 mM Tris-HCl (pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... 0.05% NP-40) and eluted with 2× Laemmli sample buffer (Bio-Rad). 30 μg of whole-cell lysate or IP elutions were resolved on 4 to 15% mini-PROTEAN TGX gels (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino- 2-phenylindole (DAPI) (1 ug/mL; Bio-Rad, Cat.# 1351303) to counterstain for confocal microscopy ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 min and bands were detected using a Chemidoc Imager (Biorad). Membranes were briefly washed with TBS-T and stripped with Replot Plus (Millipore #2504 ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for 2 hours in 5% non-fat milk (BioRad) in 1x TBS-T (10 mM tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... The reaction mixture was prepared by combining 2× ddPCR Mastermix (Bio-Rad), 20× primer and probes (final concentrations of 900 nM and 250 nM ...
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bioRxiv - Biophysics 2023Quote: ... Detergents were removed with Bio-Beads™ SM-2 resin (Bio-Rad) via two incubations at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 30 μl of SDS loading buffer (BioRad, 1610747 with 10% 2-Mercaptoethanol) was added to the beads and they were incubated at 95 °C for 5 min to elute protein complexes before using them for western blots.
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bioRxiv - Molecular Biology 2023Quote: ... staining every 2 days using a TC20 cell counter (Bio-Rad, USA). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... The detergent was removed by using Bio-Beads SM-2 (Bio-Rad) with rotation at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... Yeast chromosomal DNA served as size marker (BioRad; 200 kb – 2 Mb).
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bioRxiv - Developmental Biology 2024Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 (Bio-Rad), 0.01% Molecular Grade BSA (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... and rat anti-α tubulin (YL1/2) (MCA77G, Bio-Rad; 1:1500). Secondary antibodies used were Alexa Fluor 488 goat anti-mouse IgG (H + L ...
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bioRxiv - Molecular Biology 2024Quote: ... To the washed beads 40 µL of 2 X Laemmli buffer (BioRad) was added and incubated for 10 min at +50 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The culture supernatant was obtained by centrifugation and used with the Bio-Plex Pro Human Cytokine 8-plex Assay (Bio-rad) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... For immunohistochemistry (IHC) experiments following primary antibodies were used: IgG anti-human CD3 antibody (1:200, clone CD3-12, Bio-Rad) and anti-PCNA antibody (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Immunology 2021Quote: Recombinant human GFAP protein were run on 12% SDS-PAGE and transferred onto membrane via Trans-Blot Turbo Transfer System (BIO-RAD). Membrane was incubated in blocking buffer (PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and growth factors in culture supernatants of control or R1881 treated LNCaP cells was determined using the Bioplex Pro Human Cytokine 17-plex assay system (Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Microbiology 2019Quote: ... Isotypes of HuMAbs in culture supernatant were determined by western blot using anti–human IgG conjugated with horseradish peroxidase (HRP) (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: The details of the protein purification protocol for human plasma sample are described in the Aurum serum mini kit leaflet and a detailed was followed(BioRad, USA). The sample was prepared for 2-D gel electrophoresis following passive rehydration for 16-24 h ...
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bioRxiv - Molecular Biology 2019Quote: The SPR studies to determine conformation binding of the human chimeric 1A8 and 7C8 mAbs were carried out with the ProteOn XPR36 protein interaction array system (Bio-Rad) based on SPR technology ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Genomics 2020Quote: ... The concentrations of cytokines in plasma were measured using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit immunoassay run on the Bio-Plex 200 (BioRad), with reference to an 8 point standard curve.
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bioRxiv - Immunology 2021Quote: ... Serum was also collected for cytokine analyses which were performed by the University of Pennsylvania’s Human Immunology Core using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: Plasma from each patient sample was analyzed using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit (Bio-Rad). Cytokine expression was measured using the Luminex xMAP® (Luminex Corporation ...
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bioRxiv - Immunology 2021Quote: ... Sera were collected for cytokines analyses which were performed by the Macau University of Science and Technology using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... anti-CD63 and anti-CD81 detection antibodies (supplied in the MACSPlex kit, 5 µl each) or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647 ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected from human or mouse brain homogenates after centrifugation and the protein concentration was measured by Bradford Assay (Bio-Rad). Equal amounts of proteins were run on a 4-12% gradient SDS-PAGE gel (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: We used conjugated magnetic beads (6.5 μm) (Bio-Plex Pro Human Chemokine IL-8 / CXCL8 Set #171BK31MR2, BioRad, Hercules, CA, USA) for the binding assay ...
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bioRxiv - Microbiology 2024Quote: ... IL-28A/IFN-λ2 and IL-29/IFN-λ1) using a Bio-Plex ProTM Human Inflammation Panel 1 Express assay (Bio-Rad Laboratories Ltd. ...