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Citations for Bio-Rad :
451 - 500 of 792 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 10 µL of each protein sample was then loaded and run on precast protein gels (4-20% Mini-PROTEAN® TGXTX, BioRad) before staining with Coomassie blue dye ...
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bioRxiv - Immunology 2021Quote: ... Quantification of band intensities in 3 Western blot replicates was performed using Image Lab software (Bio-Rad). PALS-5 band intensities for each sample were normalized to the ratio of the tubulin expression levels between N2 DMSO control and a given sample.
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Microbiology 2019Quote: ... A 3 μl volume of this master mix was loaded into a 384 well plate (BioRad HSP3805) using a multichannel pipette according to the layouts in Figure 2 ...
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bioRxiv - Neuroscience 2020Quote: ... SDS-gels (3% stacking gel, 12% separation gel) were run in a Mini PROTEAN® System (BioRad) at 100 V for 10 min and 200 V till end of the separation ...
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bioRxiv - Systems Biology 2020Quote: Plates were incubated for 2-3 days at 30 °C and imaged using a ChemiDoc MP (BioRad). Individual colonies from the plates generated to enable high second stress survival quantitation were counted using in-house developed MATLAB scripts ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed 3 times and imaged by chemiluminescence (Pierce) by using a Chemidoc imaging system (BioRad). The images were further analyzed for band intensities using ImageJ software ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates from 3×106 cells were separated on 4-20 % gradient Tris-glycine polyacrylamide gels (BioRad 4568094), electroblotted onto a PVDF membrane (Pierce 88518) ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 M NaCl) for 30 minutes and the DNA was transferred to a Zetaprobe membrane (Bio-Rad) in 20X SSC ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified product was run on 3% certified™ low range ultra agarose (Bio-Rad Laboratories Ltd) in TBE buffer and a size-selection was performed to ensure that the cDNA used for sequencing primarily contains miRNAs rather than other RNA contaminants ...
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bioRxiv - Immunology 2020Quote: ... and detergent removed by adsorption to polystyrene BioBeads SM-2 (3 mg; Bio-Rad; overnight, 4°C). Proteoliposomes were collected by ultracentrifugation (180,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... the blot was rinsed 3 times in TBS-T and incubated with StrepTactin-HRP conjugate (Bio-Rad) and HRP-conjugated secondary antibody for 1 hour at 22°C ...
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bioRxiv - Microbiology 2020Quote: ... or 500 ng to 3 µg for integrative plasmids (MicroPulserTM electroporator Biorad in 2 mm cuvettes (Eurogentec) at 25 µF ...
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Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuolesbioRxiv - Cell Biology 2021Quote: ... 10–15 μL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V in 1x Tris-Acetate buffer ...
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bioRxiv - Immunology 2022Quote: ... 3 technical replicates per sample were measured on a CFX384 Touch Real-Time PCR Detection System (Biorad). Cycles were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... 10–15 µL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V to separate Rad53 isoforms ...
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bioRxiv - Molecular Biology 2022Quote: ... and added to 7 cm ReadyStrip IPG Strips with a linear pH range of 3-10 (BioRad) for passive rehydration overnight at 20 °C in a Protean IEF Cell equipment (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Isoelectric focusing was performed in immobilized pH gradient strips (17 cm, pH 3–10 NL; Bio-Rad). The focused strips were stored at −80°C until second-dimension electrophoresis was performed ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was analysed by SDS-PAGE using 3-8% Criterion XT Tris-Acetate gels (Bio-Rad) and XT Tricine running buffer (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Leaves from 3 dai (days after infiltration) were used for GFP detection by ChecmiDoc MP machine (BioRad), and ImageJ Fiji was used for quantification of GFP intensity ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBST 3 times and imaged using the ChemiDoc MP imaging system (Bio-Rad) after activation with ECL substrate solution.
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bioRxiv - Molecular Biology 2023Quote: ... ∼3 million cells were harvested and proteins were extracted by boiling in 2x Laemmli sample buffer (BioRad). Protein levels were measured by Western blotting with different antibodies (Rabbit anti- BRWD3 ...
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bioRxiv - Systems Biology 2023Quote: ... Time points were measured every 2-3 days by flow cytometry analysis of >10,000 cells (BioRad ZE5). For experiment with BRM014 (MedChemExpress #HY-119374) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were resolved in 2.5-3% agarose gel and imaged using Gel Doc EZ (Bio-Rad). Exposure time was adjusted to ensure band intensities were not saturated ...
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bioRxiv - Neuroscience 2023Quote: ... culture were then rinsed three times with PBS and treated with 3% (w/v) hydrogen peroxide (BioRad) in PBS for 5 min to minimize endogenous peroxidase activity ...
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bioRxiv - Physiology 2023Quote: ... each fiber was carefully submerged in 3 µL of lysis buffer (SingleShot Cell Lysis kit, Bio-rad), containing proteinase K and DNase enzymes to remove unwanted proteins and DNA ...
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bioRxiv - Systems Biology 2024Quote: ... 3 technical replicates per sample were measured on a CFX384 Touch Real-Time PCR Detection System (Biorad). Cycles were as follows ...
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bioRxiv - Genetics 2023Quote: ... and orange G) and ran for 3 h at 100 V with a Variable Speed Pump (BioRad) to circulate buffer during the entire gel run.
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bioRxiv - Biophysics 2023Quote: Apoptosis was probed using the Bio-Rad Magic Red Caspase 3-7 kit (Bio-Rad, ICT 935). HEK 293T cells stably expressing FGFR1-YFP were seeded in 96 well plates and allowed to grow to ∼70% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... SARS-CoV2-PP were prepared by labelling with 0.4µM DiIC18(5) solid (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt, or DID) (Thermo) with Biospin6 (Biorad) to remove residual dyes ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were then washed 3 times with TSB-T before revelation using ECL reagent (Bio-Rad 1705061) and high-resolution image acquisition using ImageQuant LAS 4000 (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2020Quote: ... smegmatis cells were transformed with 2 µL of the mycobacterial expression vector pNIT cloned with the gene Rv1630 (RpsA) using electroporation with a gene pluser (Bio-Rad, USA). Electrocompetent M ...
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bioRxiv - Molecular Biology 2019Quote: ... in a cartridge containing 20 µL of the reaction mix and 70 µL of Droplet Generation Oil for Probes (ddPCR™ 96-Well Plates #12001925, Bio-Rad). The entire emulsion volume was transferred from the cartridge to a 96-well PCR plate (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed three times for 10 minutes with 1x PBS and incubated with Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad, #172-1011). Chemiluminescence was induced by incubation with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... TMEM87A in buffer 1 was exchanged into soybean l-α-phosphatidylcholine (Soy PC, MillaporeSigma) with the addition of Biobeads SM2 (Bio-Rad) and an hour incubation at a protein:lipid ratio of 1:10 (corresponding to 0.4 mg purified TMEM87A and 4 mg of Soy PC lipid or 1:50 in buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was soaked with Chemi-Lumi One L or Chemi-Lumi One Super (Nacalai Tesque) for the signal detection using ChemiDoc XRS+ (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2022Quote: ... Milli-Q water was treated with Chelex 100 resin 100–200 mesh sodium form via batch method (50 g/L, Bio-Rad Laboratories). A concentrated stock of SD media not containing Cu2+ ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein samples were combined with the 4x Laemli sample buffer (900 μl of sample buffer and 100 μl β-Mercaptoethanol), heated (95°C, 5mins) and run on SDS-PAGE (protean TGX pre-casted gradient gel, 4%-20%, Bio-rad) and transferred to the Nitro-Cellulose membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... 20 μl of eluted protein samples were separated on SDS gel (12%) and transferred to PVDF membrane (Immun-Blot® PVDF, Bio-Rad) through semi-wet blotting protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of cDNA (10 ng of starting RNA) was amplified per reaction using the iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124) and the Bio-Rad CFX96 qPCR system.
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bioRxiv - Biochemistry 2023Quote: ... 1 μl primer mix (final concentration of 200 nM per primer) were prepared in Hard-Shell PCR 384-well plates (Bio-Rad, USA). Amplification conditions were as follows ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction collection was performed using a Masterflex L/S easy-load II (Cole Parmer, 77200-50) running at approx 1 mL/min and BioFrac™ fraction collector (Bio-Rad) with a fraction time window of 2 min ...
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bioRxiv - Microbiology 2023Quote: ... before destaining with Tris buffered saline Tween-20 (TBST) and incubation for 1 hour with blocking buffer (TBST with 50 g/L blocking agent (Bio-Rad, 1706404)) ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit IgG (H+L)-HRP conjugate (170-6510, Biorad, 1:10000) and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were incubated at 30°C for 3-5 days and photographs were taken by Chemi Doc (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... After washing 3 times with TBST the chemiluminescent was conducted by adding Clarity Western ECL Substrate (Biorad, #1705061) for 2 min and detected by ChemiDoc imaging system (Biorad) ...
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bioRxiv - Neuroscience 2019Quote: Organotypic slices were biolistically transfected at 3-5 days in vitro(div) using a Helios GeneGun (Bio-Rad), bullets were prepared using PI4KIIα shRNA alone or in combination with either wild type or S9/51A shRNA resistant PI4KIIα ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected with 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad). The CA levels from cells were normalized relative to GAPDH levels ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μg of samples 3 were mixed with 2× laemmli buffer + 5% 2-mercaptoethanol (BioRad Laboratories, Hercules, CA). Samples were then briefly vortexed and centrifuged ...