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Citations for Bio-Rad :
451 - 500 of 6069 citations for 6 methyl 4 oxo 5 6 dihydro 4H thieno 2 3 b thiopyran 2 sulfonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... was transferred to a new tube with 50 μL 2 M HCl and boiled for 2 hours at 95°C in a C1000 thermocycler (Bio-Rad) to hydrolyze all glucans to glucose ...
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bioRxiv - Microbiology 2022Quote: ... 2×2 mm squares were cut out and placed into double-sided sticky 9×9 mm frame seals (Bio-Rad SLF0201) on a glass slide ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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bioRxiv - Cell Biology 2022Quote: ... Excess dye was removed by two consecutive buffer exchanges through Micro Bio-Spin P-6 desalting columns (Bio-Rad). Protein concentration and labeling efficiency were estimated using a NanoDrop Microvolume Spectrophotometer.
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bioRxiv - Genomics 2019Quote: Binding reactions were incubated at room temperature for 30 min and loaded onto a 6% polyacrylamide 0.5 × Criterion precast TBE gel (Biorad). After sample electrophoresis and transfer to a nylon membrane ...
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bioRxiv - Biochemistry 2020Quote: ... 0.14 M NaCl (PBS) followed by removal of the reductant using a Micro Bio-Spin 6 column (Bio-Rad). Columns were washed and pre-equilibrated with 20 mM phosphate buffer pH 7.4 containing 0.1 mM diethylenetriamine-penta-acetic acid (DTPA ...
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bioRxiv - Biochemistry 2021Quote: ... individual proteins were buffer exchanged into 200 mM ammonium acetate pH 8.0 either by SEC or using Biospin-6 (BioRad) columns and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... The annealed doubly nicked DNA duplex was then buffer-exchanged in Hepes 20 mM pH 7.5 with a BioSpin 6 column (BioRad).
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bioRxiv - Microbiology 2022Quote: All proteins were buffer exchanged into 0.2 M ammonium acetate using Bio-Spin P-6 Size Exclu-sion Spin Columns (BioRad) as previously described47 ...
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bioRxiv - Genetics 2022Quote: ... PMOs were buffer exchanged into 25 mM ammonium bicarbonate buffer pH 7.5 using Biospin 6 (Bio-rad, Hercules, California) columns according to the manufacturer’s protocol and then spinning through an 0.22 µm cellulose acetate centrifugal spin filter ...
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bioRxiv - Genetics 2019Quote: ... 10 mM L-serine) to a UapA concentration of 20 μM using Micro Bio-Spin 6 columns (Bio-Rad). UapA was loaded into gold coated capillaries and the protein sprayed into a Synapt G2-Si (Waters ...
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bioRxiv - Neuroscience 2019Quote: ... and each qPCR reaction was performed with 6 ng cDNA and Bio-Rad IQ SYBR Green Supermix (Bio-Rad CFX384 Touch Real Time PCR Detection System ...
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bioRxiv - Biochemistry 2019Quote: ... The annealed doubly nicked DNA duplex was then buffer-exchanged in Hepes 20 mM pH 8.0 with a BioSpin 6 column (BioRad).
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bioRxiv - Evolutionary Biology 2020Quote: ... The Chlorella DNA was extracted by isolating the Chlorella and then using a standard 6% Chelex100 resin (Bio-Rad) extraction method ...
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bioRxiv - Biochemistry 2019Quote: ... Buffer exchange or removal of excess NTPs and abortive products were performed by gel filtration (BioRad Micro-Spin 6), if necessary.
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bioRxiv - Biophysics 2019Quote: ... Gel precursor solutions were prepared with 6% v/v acrylamide and 0.2% v/v bisacrylamide (30% acrylamide/bisacrylamide stock 29:1, BioRad), 2 mM LAP photoinitiator (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories). Protein (final concentration 20 µM ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Excess free Texas Red or TAMRA were removed with Bio-spin 6 kDa size exclusion columns (Bio-Rad Laboratories) equilibrated with PBS according to manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (Healthcare Life Sciences) were loaded into Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Genomics 2023Quote: ... Excess dye was removed using Micro Bio-Spin® Columns with Bio-Gel® P-6 (cat:7326221, BioRad) by centrifugation at 1000g ...
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bioRxiv - Genetics 2023Quote: ... followed by purification and size selection on 1% agarose gel with .6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...
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bioRxiv - Synthetic Biology 2023Quote: ... The reactions were run at 125 V for 2.5 hours on a denaturing (6 M urea) 20% (w/v) polyacrylamide gel in 50 mM NaOAc pH 5.2 running buffer (using the Biorad Mini- PROTEAN tetra gel ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 400 µL cold PBS and electroporated with 6 µg of RNA in 0.4-cm electroporation cuvettes (Biorad) with a 950 μF and 260 V pulse using Genepulser system (Biorad) ...
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bioRxiv - Plant Biology 2023Quote: ... Samples containing 6 µg total protein were resolved using pre-cast SDS-PAGE Any KD™ gels (BIO-RAD), transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 µm ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated at 175 V for 3-4 hours at 4°C and then transferred to nitrocellulose membrane (BioRad) by using a Criterion blot cell (BioRad ...
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bioRxiv - Plant Biology 2021Quote: ... 3 μL cDNA template and 4 μL iQ SYBR green supermix (Bio-Rad) in a total reaction volume of 12 μL ...
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bioRxiv - Cell Biology 2023Quote: ... and resolved by SDS-PAGE using Criterion XT Bis-Tris precast gels (4-12%; BioRad, 3450123/4/5) and XT-MES1X as running buffer (stock 10X ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg of lysate was heat denatured for 3 minutes at 95°C for 3 minutes and resolved on a pre-cast 4-15 % SDS-PAGE gels (Bio-Rad) at 200V for 45 minutes in 1X running buffer (25mM Tris pH 8.3 ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... TGFβ2 and TGFβ3), as 2 separate dosages, following supplier (Merckmillipore, Burlington, MA, USA) guidelines using a Bioplex 200 (BioRad, Hercules, CA, USA). The quantification of all samples was performed all together.
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Plant Biology 2019Quote: ... Bio-Beads SM-2 absorbents (152-8920, Bio-Rad) that had been washed in methanol (3 x 10 min) ...
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bioRxiv - Biophysics 2021Quote: ... 1.5 ml of BioBeads SM-2 slurry (Bio-Rad) was added to remove the detergents ...
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bioRxiv - Biophysics 2021Quote: ... After several incubation rounds with biobeads SM-2 (Biorad), the proteoliposomes were harvested the next day ...
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bioRxiv - Neuroscience 2021Quote: ... 2 million N2A cells were transfected using TransFectin (Biorad) with either CFP-ctrl. ...
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bioRxiv - Immunology 2019Quote: ... Fixed HEp-2-coated microscope slides (Kallestad®, BioRad) were blocked ...
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bioRxiv - Genetics 2019Quote: ... One volume of 2% low melt agarose (Bio-Rad) in TSE was added and the suspension dispensed into plug molds ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were run on an Optocon 2 thermocyler (Biorad). Primers used are listed in Table 2.
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bioRxiv - Biophysics 2021Quote: ... 37.5 μl 2% Bis-solution (161-0142, Bio-Rad), 2 μl (0.4% ...
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bioRxiv - Bioengineering 2021Quote: ... Synthetic SARS-CoV-2 RNA was purchased from BIORAD (SARS-CoV-2 Standard #COV019 and SARS-CoV-2 Negative #COV000).
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bioRxiv - Neuroscience 2021Quote: ... treated with 2 X Laemmli sample buffer (Bio-Rad) to elute/denature protein before SDS-PAGE and immunoblotting for DAT as above ...
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bioRxiv - Cell Biology 2021Quote: ... 12.5 μl 2× ddPCR Supermix for probes (Bio-Rad), 1.25 μl R636S+SM FAM probe and primer premixture ...
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bioRxiv - Biophysics 2020Quote: ... An equal volume of Biobeads SM-2 (Bio-Rad) was added to the nanodisc mixture for detergent removal ...
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bioRxiv - Molecular Biology 2022Quote: ... in a 2 mm Gene Pulser cuvette (Bio-Rad) and was subjected to electroporation at 2.3 kV ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 mM 2-Mercaptoethanol (Bio-Rad Cat#1610710), and nucleosides (Millipore Cat#ES-008-D).
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bioRxiv - Molecular Biology 2020Quote: ... 2) Chelex 100 Resin (Bio-Rad, Hercules, CA, USA); and 3 ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 M thiourea and 65 mM dithiothreitol (BIORAD, Australia). Isoelectric focusing (IEF ...