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Citations for Bio-Rad :
451 - 500 of 747 citations for 6 Chloro 2H chromene 3 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were resolved in 2.5-3% agarose gel and imaged using Gel Doc EZ (Bio-Rad). Exposure time was adjusted to ensure band intensities were not saturated ...
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bioRxiv - Neuroscience 2023Quote: ... culture were then rinsed three times with PBS and treated with 3% (w/v) hydrogen peroxide (BioRad) in PBS for 5 min to minimize endogenous peroxidase activity ...
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bioRxiv - Physiology 2023Quote: ... each fiber was carefully submerged in 3 µL of lysis buffer (SingleShot Cell Lysis kit, Bio-rad), containing proteinase K and DNase enzymes to remove unwanted proteins and DNA ...
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bioRxiv - Systems Biology 2024Quote: ... 3 technical replicates per sample were measured on a CFX384 Touch Real-Time PCR Detection System (Biorad). Cycles were as follows ...
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bioRxiv - Genetics 2023Quote: ... and orange G) and ran for 3 h at 100 V with a Variable Speed Pump (BioRad) to circulate buffer during the entire gel run.
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bioRxiv - Biophysics 2023Quote: Apoptosis was probed using the Bio-Rad Magic Red Caspase 3-7 kit (Bio-Rad, ICT 935). HEK 293T cells stably expressing FGFR1-YFP were seeded in 96 well plates and allowed to grow to ∼70% confluency ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was analysed by SDS-PAGE using 3-8% Criterion XT Tris-Acetate gels (Bio-Rad) and XT Tricine running buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were separated on 6-10 % SDS-polyacrylamide gels or 4-20 % Mini-PROTEAN TGX precast gradient gels (Bio-Rad, CA, USA) together with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... and their respective complexes with Hap40 protein were buffer-exchanged into 150 mM aqueous ammonium acetate (pH=7.5) by using P-6 Bio-Spin gel filtration columns (Bio-rad, Veenendaal, the Netherlands). The protein’s resulting concentration was estimated to be ~2-5 μM before native MS analysis ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Genetics 2022Quote: ... The DNA was diluted to 6 ng/µl for copy number screening and qPCR was performed using SsoAdvanced™ Universal SYBR® Green Supermix (Biorad, 1725272) and 1µM primer mix to amplify the target within and flanking the deleted region ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (10-100 μg proteins/lane) were loaded to and separated on 6%-15% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620177, Bio-Rad, Hercules, CA). The membranes were blocked in 4% BSA/PBST (0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2022Quote: ... was performed for 23 h at 14°C with an initial switch time of 60 s and a final switch time of 120 s at 6 V/cm and an included angle of 120° as described previously (Bio-Rad CHEF Mapper) [35] ...
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bioRxiv - Physiology 2023Quote: ... 18 and 21 months of age and were subjected to IEF using a 24 cm immobiline DryStrip (pH 6-11) (BIO-RAD, Hercules, CA), followed by SDS-PAGE using pre-casted 10-16% gradient polyacrylamide gels (BIO-RAD ...
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bioRxiv - Biophysics 2023Quote: Proteins samples were kept on ice and buffer exchanged into 200 mM ammonium acetate buffer at pH 7 using Micro Bio-spin columns with either a 6 or 30 kDa cutoff (Bio-Rad, Hercules, CA). Buffer exchanged samples were then diluted to a working concentration of 10 μM before analysis ...
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bioRxiv - Immunology 2024Quote: ... ACPA IgG levels were quantified based on an in-house standard of pooled RA patient plasma using the Microplate manager software MPM-6 (Bio-Rad Laboratories, Inc.). Unspecific reactivity to the peptide backbone was assessed by applying CArgP2 ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were incubated at 30°C for 3-5 days and photographs were taken by Chemi Doc (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... After washing 3 times with TBST the chemiluminescent was conducted by adding Clarity Western ECL Substrate (Biorad, #1705061) for 2 min and detected by ChemiDoc imaging system (Biorad) ...
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bioRxiv - Neuroscience 2019Quote: Organotypic slices were biolistically transfected at 3-5 days in vitro(div) using a Helios GeneGun (Bio-Rad), bullets were prepared using PI4KIIα shRNA alone or in combination with either wild type or S9/51A shRNA resistant PI4KIIα ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected with 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad). The CA levels from cells were normalized relative to GAPDH levels ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μg of samples 3 were mixed with 2× laemmli buffer + 5% 2-mercaptoethanol (BioRad Laboratories, Hercules, CA). Samples were then briefly vortexed and centrifuged ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 μg of protein was separated on 4% to 20% precast polyacrylamide gels (Bio-Rad, cat # 4561095) for detection of histone H3 andH3K27me3 ...
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bioRxiv - Biochemistry 2020Quote: ... The purified MCU-EMRE subcomplex in DDM was then reconstituted into nanodisc by incubating with Msp1 protein, lipids (POPC:POPE:POPG, 3:1:1 molar ratio) and BioBeads (BioRad) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... and plates were read 3 times at 450 nm in the model microplate ELISA reader (BIO-RAD, Japan). Negative controls (coated with naive sera ...
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bioRxiv - Neuroscience 2019Quote: ... then 3 washes in 1% milk were performed prior to imaging the blot with ECL (BioRad Clarity #1705060). Membrane was stripped using the GM Biosciences One Minute Advance Stripping buffer #GM6031 ...
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bioRxiv - Neuroscience 2019Quote: ... Beads were then washed 3 times with IP buffer before adding 2x Laemmli buffer (Biorad, Hercules, CA, USA). Samples were analyzed by SDS-PAGE and Western blotting.
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bioRxiv - Biochemistry 2021Quote: ... Membranes were again washed 3 times in PBST before being incubated with Clarity™ ECL reagents (Bio-Rad) or SuperSignal™ West Femto Maximum Sensitivity Substrate and visualized (see S11 Fig for full length immunoblots).
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bioRxiv - Neuroscience 2020Quote: ... Three replicas of 1.5μl of a 1:3 dilution of cDNA were amplified using SsoFast EvaGreen Supermix (BioRad) for FACS-sorted microglia and Power SYBR Green (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories). Protein was transferred to a nitrocellulose membrane (Hybond ECL ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed 3 times in TBSt and then incubated with HRP secondary antibodies (anti-Mouse HRP, Biorad, 170-6516 ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7) and Western blotting samples were prepared by adding sample buffer (3× Laemmli Sample Buffer 1610747; BioRad) plus 3.57 M β-mercaptoethanol (2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... The purified 3-cycle PCR products were qPCR quantified using Platinum SuperFi II on a CFX96 system (BioRad). The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat ...
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bioRxiv - Plant Biology 2022Quote: ... 12.5 mM imidazole) for 3 times and boiled at 95°C with 30 μl SDS loading dye (BioRad). After removing the beads ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBST (3 times) and developed with ECL western blot substrate (Bio-Rad) and imaged using Azure C300 imaging system (Azure biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: 4–8 μl of samples and 3 μl of Precision Plus Protein Dual Color Standard (1610374, Bio-Rad) were loaded into 4–12% Bis-Tris Bolt gels (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were blocked overnight at 4°C in PBS with 3% BSA and 10% donkey serum (Bio-Rad), stained with DAPI (ThermoFisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Molecular Biology 2023Quote: ... we performed 3 washes with PBST for 10 minutes before imaging the membrane on a ChemiDoc system (BioRad). The original scans of the blots are provided in Supplemental Table 3.
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bioRxiv - Biochemistry 2023Quote: ... membranes were washed 3 times in TBS-T and incubated for 45 minutes with secondary antibody (Bio-Rad: Goat Anti-rabbit #1706515 or Goat-anti mouse #1706516) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used 1 μL diluted cDNA (1:3 in ddH2O) and iTaq Universal SYBR Green Supermix (Bio-rad) in the Bio-rad CFX96 real-time PCR detection system for qPCR experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were incubated for 3 days at 30 °C and imaged in a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... The supernatant from two reactions were collected and separated by Criterion XT Tris Acetate 3-8% (Bio-rad) SDS-PAGE and visualized by silver staining.
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bioRxiv - Immunology 2024Quote: ... cDNA was mixed with appropriate primers (Supplementary Table 3) and SYBR green master mix (BioRad, catalog no. 1708882) and run on a Thermocycler T100 (BioRad) ...
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bioRxiv - Biophysics 2021Quote: ... and 3.6 μL ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The 2-ΔΔCt method was used for the quantitative analysis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was extracted from several colonies of a 6-week-old subculture on Coletsos culture medium using the InstaGene matrix following the manufacturer’s instructions (Bio-Rad, Marnes-la-Coquette, France). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting transcripts were treated with DNase for 20 min at room temperature and then isolated using Micro Bio-Spin™ 6 Columns (BioRad, Cat.# 732-6221). 0.75 µg of RNA transcripts were mixed with 2X formamide buffer (95% deionized formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the SL2-6 construct was subsequently size purified using a denaturing polyacrylamide gel (7M Urea, 1x TBE, hand-poured in Bio-Rad Criterion midi cassettes), loaded in 80% formamide ...
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... was run with a 1% agarose TBE gel over 24 hours (Initial switch 1 sec, final switch 25 sec, 6 volt/cm, 120° included angle, Chef III, Bio-Rad, Hercules, CA, USA) with Midrange PFG and Lambda PFG markers (N0342S ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3 times for 10 minutes each and coated with enhanced chemiluminescence (ECL; Clarity ECL, BioRad). Using X-ray film ...