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Citations for Bio-Rad :
451 - 500 of 6783 citations for 2 3 5 Dioxo 4 aza tricyclo 5.2.1.0*2 6* dec 8 en 4 yl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were boiled at 95°C for 5 min and proteins were separated on 4–15% Mini-Protean TGX precast protein gel (Bio-Rad) and transferred onto hydrophobic polyvinylidene fluoride (0.45 μm PVDF ...
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bioRxiv - Immunology 2024Quote: ... were washed with 500 μl ice-cold acetone to remove TCA at 16,000g for 5 min at 4 °C and resuspended in 100 μl 1x XT sample buffer (Bio-Rad #1610791), boiled at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were boiled for 5-10 minutes and then separated using SDS-PAGE with 4-12% Criterion XT Bis-Tris Gels (Bio-Rad). The proteins were transferred to PVDF membranes (Merck Millipore) ...
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bioRxiv - Plant Biology 2024Quote: ... heat-denatured at 95°C for 5 min and separated by SDS-PAGE on home-made gels or 4-15% precast polyacrylamide gel (Bio-rad).
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bioRxiv - Neuroscience 2024Quote: ... 50 μg protein/sample was denatured at 95 °C for 5 min in Laemmli buffer with 2.5% ß-mercaptoethanol and separated with a 4–20% Mini-PROTEAN (Bio-Rad) polyacrylamide gel in 25 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... heated at 95 °C for 5 min and loaded on stain-free 4–15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis gels (Bio-Rad). Separated proteins were transferred onto a Polyvinylidene difluoride (PVDF ...
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bioRxiv - Genomics 2024Quote: ... the samples were treated with 5% 2-mercaptoethanol in Laemmli buffer and heated at 95°C for 5 minutes prior to loading into a 4-15% gradient SDS gel (Cat# 4568085, Bio-Rad) and run at 160v for 50-55 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled at +90 °C for 5 min prior to protein separation using precast SDS- PAGE gradient gels (4–20% Mini-PROTEAN TGX, Bio-Rad) and transferred onto nitrocellulose membranes with the semi-dry Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... the concentration of the resultant solution containing labelled αSyn was adjusted to 2 mg/mL and excess unbound dye was removed using Bio-Spin 6 size exclusion spin columns (Bio-Rad Laboratories). ATT565-αSyn was added to LUHMES cells on day six in vitro at a final concentration of 2 µM ...
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bioRxiv - Physiology 2021Quote: ... Relative protein abundance (N=6-8) were quantified using the Image Lab software (version 6.0.1; BioRad) and normalized by the protein content in each lane.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were boiled in 4× Laemmli buffer for 10 minute and resolved on a precast 4 to 15 % polyacrylamide gel (Bio-Rad). Proteins were transferred to nitrocellulose using a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... before separation by SDS PAGE using commercial gels (NuPAGE Novex 4-12% Bis-Tris gels (Thermo-Fisher-Scientific) or Mini-PROTEAN TGX 4-20% gels (Bio-Rad)) and transfer to 0.2 µm nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: Electrophoresis was performed using a BioRad Mini-PROTEAN cell with 4-12% or 4-15% precast TGX gels (BioRad, Hercules, CA) in TGS running buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein extract was then added to the mixture and gently rotated at 4°C overnight then beads were washed 4 times with CHAPS buffer and then boiled in Laemmli Sample Buffer (Bio-Rad) for 10 min ...
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bioRxiv - Bioengineering 2021Quote: Whole cell lysates and EVs (4 × 108 EVs) were derived from PalmReNL-HEK293FT cells and mixed with 4× sample buffer (Bio-Rad) with β-mercaptoethanol for detecting Alix ...
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bioRxiv - Cell Biology 2023Quote: ... Fyn-/- lysate was loaded (4 sets per gel) on 4-20% TGX precast Mini-PROTEAN gels (4561103, BIO-RAD, Hercules, CA) and run at 100 V ...
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bioRxiv - Cancer Biology 2024Quote: ... β-mercaptoethanol for 5 minutes at 95°C and loaded onto precast 4-20% or 4-15% polyacrylamide mini-PROTEAN Tris-Glycine eXtended (TGX) gels (BioRad, UK). Proteins were transferred onto either 0.2 μm pore-size Nitrocellulose membranes or methanol-activated 0.45 μm pore-size PVDF membranes (BioRad ...
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bioRxiv - Biophysics 2020Quote: ... before being transferred to Whatman 3 MM paper and dried (50°C, 2 hours) using a gel dryer (Model 583, BioRad) attached to a vacuum pump ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3 times with PBS-T and developed with ECL (Biorad170-5060) for 2 min and imaged on ChemiDocTMMP (Biorad).
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bioRxiv - Genomics 2020Quote: Lysates were separated by SDS-PAGE on 3-8% Tris-acetate gels (BioRad #3450129) for 2.5 hours at 150V ...
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bioRxiv - Microbiology 2023Quote: ... samples were run through Criterion XT Tris-acetate precast gels (3-8% gradient) (Biorad) in a XT tricine buffer (Biorad) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for DpnII digested DNA or primers 3 and 4 (table S3) for NlaIII digested DNA on a thermal cycler (Bio-Rad C1000 Touch) with the block settings shown in table 1 ...
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bioRxiv - Microbiology 2024Quote: ... The hypervariable region V3–V4 of the bacterial 16S rRNA gene was amplified using the primers 338F (5’-ACTCCTACGGGAGGCAGCAG-3’)/ 806R (5’-GGACTACHVGGGTWTCTAAT-3’) by using a T100 Thermal Cycler PCR thermocycler (BIO-RAD, USA). The PCR reaction mixture (20 µL ...
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bioRxiv - Cancer Biology 2024Quote: ... the plate was held at 4 °C until the lid temperature cooled to 37 ºC and could be stored at 2 °C to 8 °C overnight before analysis on the QX600 Droplet Reader (Bio-Rad Laboratories, California, USA).
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bioRxiv - Immunology 2020Quote: ... the reaction mixture was passed through 2 Bio-Gel P-2 mini columns (1504114, Bio-rad) by centrifugation (3000 rpm ...
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bioRxiv - Microbiology 2020Quote: ... 20 μL of normalized lysates were loaded into pre-cast 4-20% SDS-PAGE gels (4-20% Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad) alongside size standards (Precision Protein Plus™ ...
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bioRxiv - Cell Biology 2023Quote: Proteins were resolved by SDS-PAGE using 4-20 % or 4-15% TRIS-glycine gradient midi gels (Bio-Rad, Hercules, CA, USA), in electrode buffer (0.1% w/v SDS ...
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bioRxiv - Biophysics 2024Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 8–25 μg protein with 6x Laemmli buffer was loaded onto 4-15% or 4– 20% Mini-PROTEAN TGXTM Precast Protein Gels (Bio-Rad, 4561084) or 8 or 12% self- casted acrylamide/bisacrylamide (29:1 ...
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bioRxiv - Genetics 2021Quote: ... Forty micrograms of total protein from hippocampal nuclear lysates was boiled for 5-minutes to dissociate complexes before separation in a 4-20% gradient gel (Bio-Rad 4561096) for 1-hour at 150V in 1x Tris/Glycine/SDS (Bio-Rad 1610732) ...
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bioRxiv - Cell Biology 2022Quote: Protein homogenates (5 or 15 μg/lane) were size fractionated on 4–15% or 15% Criterion TGX gels (Biorad Laboratories, Oslo, Norway) and transferred to 0.45 μM PVDF-membranes (GE Healthcare) ...
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bioRxiv - Cancer Biology 2020Quote: ... To reduce non-specific binding single cell suspensions were incubated for 5 min at 4°C with PBS/10% AB-serum (Bio-Rad, Germany), subsequently stained with fluorescence-labeled or biotinylated antibodies for 15 min at 4°C and washed once with PBS/2% FCS/0.01% NaN3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated to 95°C for 5 min and then analyzed on a 4-12% gel (Bio-Rad Laboratories, Hercules, CA) using SDS running buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples are prepared for SDS-PAGE by adding 5 μl of sample to 4 μl of tricine sample buffer (Bio-Rad 1610739) and 1 μl of 1 M DTT and heat denaturing at 70 °C for 3 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were prepared for SDS-PAGE by adding 5 μl of sample to 4 μl of tricine sample buffer (Bio-Rad 1610739) and 1 μl of 1 M DTT and heat denaturing at 90 °C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled at 95°C for 5 min and loaded into 4-12% Criterion XT-Bis-Tris polyacrylamide gels (Bio-Rad 3450125). Gel electrophoresis was performed in 1X MES running buffer (Bio-Rad 1610789 ...
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bioRxiv - Molecular Biology 2023Quote: ... Table 1) was performed at 4°C overnight at a 1:1000 dilution in 5% nonfat dry milk (Bio-Rad No. 1706404). Incubation of HRP conjugated secondary antibody (different species ...
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bioRxiv - Biochemistry 2024Quote: ... and boiled at 95° C for 5 minutes before separation on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). In-gel fluorescence was imaged first ...
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bioRxiv - Microbiology 2024Quote: ... protein samples were boiled at 95 °C for 5 min and separated by a 4–20% Mini-PROTEAN® TGX Stain-Free™ SDS PAGE (BioRad) or 12 % Tris-Glycine SDS PAGA (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... complemented with 2-mercaptoethanol (Bio-rad) and heated at 95 °C for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 2-mercaptoethanol (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... Bio-Beads SM-2 (Bio-rad) were supplemented into the mixture to remove the detergent ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with 2-mercaptoethanol (Bio-Rad) and subjected to western blotting (WB ...
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bioRxiv - Biophysics 2021Quote: ... bio-beads SM-2 (Bio-Rad) were added to the mixture and rotated overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 25 μL bis-acrylamide 2% (BioRad), 137 μL acrylamide 40% (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... 20 μL bis-acrylamide 2% (BioRad), 70 μL acrylamide 40% (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... 2×Laemmli buffer (Bio-Rad Laboratories) supplemented with β-mercaptoethanol was added to the beads ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µM 2-mercaptoethanol (Bio-Rad), DMEM/F-12 (Invitrogen) ...