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Citations for Bio-Rad :
451 - 500 of 8761 citations for 1 Benzyl 4 5 benzyloxy 6 methoxy 1 indanone 2 ylidenyl methylpiperidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then rinsed 3 times with PBS to ensure the complete removal of glutaraldehyde and blocked for 1 h in blocking buffer (5% milk (Bio-Rad #1706404) in Tris-Buffered Saline (TBS ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then blocked for 1 hour in 5% non-fat milk in PBS containing 0.1% Tween 20 (Bio-Rad, Hercules, CA) at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The qPCR reaction was carried out on 2μL of 1/5-diluted cDNA using 2X SSoAdvance SYBR Super Mix (Bio-Rad, Hercules CA) with locus-specific primers (Table S2).
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bioRxiv - Synthetic Biology 2024Quote: ... the membrane was washed using TBST and incubated for 1 h in a 1:20,000 dilution in 5 % milk of Immun-Star Goat Anti-Mouse (GAM)-HRP conjugate from Bio-Rad (Ontario Canada). Three washes were done ...
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bioRxiv - Cell Biology 2024Quote: A total of 10 µg of protein was combined with 5 µL of Laemmli buffer (1:9 β-mercaptoethanol [PCS 1610710, Bio-Rad] to Laemmli sample buffer [PCS 161-0737 ...
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bioRxiv - Neuroscience 2021Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 5% milk/PBST at RT ...
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bioRxiv - Cell Biology 2020Quote: ... and 1:500 to 1:1000 anti-rabbit (BioRad, 172–1019). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and F4/80 (dilution 1:200, clone A3-1, Bio-Rad); ER2 EDTA for CD3 (dilution 1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 0.1% Tween-20 in 1x PBS (PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-Gapdh antibody, BioRad) in 5% milk/PBST at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were mixed 1:1 with 2x Laemmli sample buffer (BioRad) supplemented with 5% □-ME (Sigma ...
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bioRxiv - Genetics 2023Quote: ... and mixed 1:1 with 2X Sample Loading Buffer (Bio-Rad) + 5% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for 1 hour in 1% casein (Bio-Rad) in PBST (0.1% Tween-20 in PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... at 1:2000 in filtered PBS with 1% casein (Biorad 1610783). Goat-anti-mouse IgM secondary antibody (CalBiochem cat# 401225 ...
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bioRxiv - Molecular Biology 2020Quote: ... for 2 h at room temperature and a HRP-conjugated goat anti-rabbit secondary antibody (Bio-Rad 1706515; 1:3000 dilution) for 1 h at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were resolved electrophoretically on ethidium bromide stained-2% agarose gel immersed in 1× TAE and visualized with Biorad Gel Doc XR instrument (Biorad, USA).
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bioRxiv - Biophysics 2021Quote: ... The DDM/CHS detergent was then removed by overnight incubation with Bio-Beads SM-2 (15 mg per 1 ml protein/detergent/amphipols mixture, Bio-Rad) at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
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bioRxiv - Biochemistry 2022Quote: An equivalent volume of ∼150 µg FBS was first diluted 1:2 with loading buffer (consisting of 2X Laemmli Sample Buffer (#1610737, Bio-Rad) plus a 5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... Viability of magnetically-captured cells (eluted into a new tube away from the magnet) was assessed by trypan blue staining (1:2 dilution sample in trypan blue; BioRad 1450013). Cell viability was quantified as the proportion of cells that did not take up the dye ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and incubated for 2 h with the appropriate HRP-conjugated secondary mouse antibody (1/1500, BioRad Laboratories) or secondary goat anti□rabbit IgG HRP (1/5,000 Cusabio Cedarlane ...
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bioRxiv - Physiology 2024Quote: ... The cells were centrifuged at 1500 rpm for 10 mins at 4°C and the pellet was resuspended in 5 ml single-cell suspension buffer (2 mM EDTA, 0.5 BSA, 1% FBS in DPBS) and the cells were counted (Bio-Rad TC20) to prepare 1×106 aliquots.
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized starting from 1-2 μg of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, catalog #1708891). RT-qPCRs were carried out with the Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM borate pH 10 was prepared at 5 μM and loaded onto a 4-20% Mini-Protean TGX pre-cast gel (BioRad). 10 μL of each folding sample was subsequently loaded onto the same gel ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed with IP lysis buffer three time and boiled in 25 ul of 2X SDS-loading buffer for 5 minutes and loaded into 4-15% polyacrylamide gels (BioRad).
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bioRxiv - Neuroscience 2022Quote: ... heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (4 –15% Tris-HCl; BioRad) and transferred onto Immun-Blot PVDF 0.2 µm (BioRad ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Microbiology 2022Quote: ... pBAC-BoHV-4-A-revORF45HA and pBAC-BoHV-4-A-ΔORF45KanaGalK DNAs (5 μg) were electroporated in 600 μl DMEM high without serum (Biorad, Gene Pulser XCell ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed in 4 x 5 minutes in 0.2% TBS tween before incubating in Clarity™ Western ECL Substrate (BioRad) for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... boiled for 5 min at 95 °C and then loaded on 4–15% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Gels were transferred onto polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... before heating at 95°C for 5 minutes and finally loaded onto 4-20% polyacrylamide gel (Bio-Rad, Cat # 4561094). Samples were then transferred to a PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed again after secondary antibody incubation and developed using either nitroblue tetrazolium (NBT)–5-bromo-4-chloro-3-indolylphosphate (BCIP) for AP-conjugated secondary antibodies or the ECL substrate (BioRad) for the HRP labelled secondary antibody after incubating for about 10 to 15 mins at room temperature with gentle shaking ...
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bioRxiv - Cell Biology 2020Quote: ... Total numbers of viable cells were determined after 2 and 4 days using the TC10 cell counter (Bio-Rad).
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 μl of the cDNA sample were supplemented with 5 μL of SsoADV Universal SYBR Green Supermix (BioRad) mix 2X ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Neuroscience 2024Quote: ... Microplate manager 6 software (MPM6 Biorad) was used for plate reading.
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated with the primary antibodies (rabbit anti-cPLA2, 1:100, Cell Signalling; mouse anti-LaminA/C, 1:500, ABCAM; rat anti-tyrosinated α-tubulin 1:500, Bio-Rad; mouse γ-H2AX, 1:2000) in blocking solution for 1h at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with horseradish peroxidase conjugated secondary antibodies (1:5,000 or 1:10,000 in non-fat dry milk, 1 h, room temperature; Bio-Rad Laboratories). Secondary antibodies were visualized using enhanced chemiluminescence (ECL Plus ...