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Citations for Lumiprobe :
51 - 100 of 109 citations for Cyanine2 NHS ester minimal dye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... by adding an equimolar amount of Cyanine7 NHS ester (Lumiprobe, Hunt Valley, md, USA) to the lipid dissolved in 0.1 M aqueous sodium bicarbonate buffer (pH 8.5) ...
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bioRxiv - Bioengineering 2023Quote: ... Fluorescein isothiocyanate (FITC) and cyanine-5 succinimidyl ester (Cy5-NHS) were purchased from Lumiprobe Corporation (Shanghai ...
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bioRxiv - Immunology 2021Quote: ... slides were stained with human VRC01 mAb directly labeled with AF488 NHS ester (21820, Lumiprobe). For non-permeabilized sections ...
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bioRxiv - Biochemistry 2022Quote: ... DNA was mixed with an 8-fold molar excess of Cyanine5 NHS-Ester (Lumiprobe #23020) and incubated overnight at ambient temperature ...
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bioRxiv - Bioengineering 2024Quote: EVs were labeled with sulfo-Cyanine5.5 NHS ester or sulfo-Cyanine5.5 carboxylic acid (Lumiprobe, 27390) to assess the biodistribution of the EVs ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated with either 1 µM AF 488 NHS-ester (Lumiprobe, Cat. No. 11820) conjugated CbpF nanodiscs or AF 488 NHS-ester conjugated nanodiscs at 37 °C and 150 rpm for 2 hours ...
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bioRxiv - Bioengineering 2024Quote: ... Modification of the Wnt-surrogate with an azide-moiety was achieved using an azidobutyric-NHS ester (Lumiprobe) according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2024Quote: ... The HA was then chemically labeled at a ratio of approximately one dye molecule per HA trimer using Sulfo-Cy5 NHS dye (Lumiprobe). Sequences of constructs used in this work are given in Supplementary Information.
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bioRxiv - Biophysics 2020Quote: ... The downstream-sense (amino modifier C6 dT) was labeled with Cy3-NHS ester (Lumiprobe, Hunt Valley, MD, USA) at +2 position of the non-template strand (downstream-senseCy3) ...
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bioRxiv - Biochemistry 2022Quote: ... These samples were then mixed at a molar ratio of 1:1 with Cyanine3 NHS-Ester (Lumiprobe #21020) and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... BDP FL fluorophore-labelled attacins were made using an amine reactive BDP-FL NHS ester (Lumiprobe, Hunt Valley,MD). The fluorophore conjugation was carried out using the manufacturer provided protocol ...
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bioRxiv - Immunology 2022Quote: ... 5 mg of mAb in 100 μL of PBS was incubated with 10 μL of filter-sterilized 1M sodium bicarbonate and 1 μL of 10 mg/mL AF488 NHS-ester (Lumiprobe) for 1 hr ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... DNA oligonucleotides /5AmMC6/GGGATAAGTTGATTGCAGAGC-3’(anti-handle) were modified with a 5’ amine and covalently coupled to Cy5 fluorophores via N-hydroxysuccinimide (NHS) ester coupling (Lumiprobe). DNA oligonucleotide (1 mM in ddH2O ...
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bioRxiv - Biophysics 2024Quote: ... Cy3b-maleimide (catalog no. 11525834) was obtained from Cytiva. Alexa Flour 647-NHS ester (catalog no. 16820) was obtained from Lumiprobe. ATTO647N-maleimide (catalog no ...
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bioRxiv - Immunology 2024Quote: ... HPLC purified DNA single strand equipped with a 3’-aminohexyl-linker was conjugated to a sulfo-Cyanin5 NHS ester (Lumiprobe). Samples were quantified by measuring the UV absorption at 260 nm (based on the theoretical extinction coefficient ...
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bioRxiv - Bioengineering 2024Quote: PLR was solubilized at 100 mg/mL in diH2O then mixed with 2 molar equivalents of BDP-TR-NHS-ester (Lumiprobe) in DMSO to generate a 15 mg/mL PLR solution ...
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bioRxiv - Biochemistry 2024Quote: ... 6HN-GST-Tue1 and 6HN-GST-Tue16 protein were labeled with Alexa Fluor® 488 (NHS Ester, Lumiprobe GmbH, Germany). Recombinant 6HN-JAZ10 (3,1nM-100 μM ...
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bioRxiv - Bioengineering 2023Quote: ... Rat tail collagen type I (at final concentration 2.5 mg/mL and pH 7.4) and hyaluronic acid (0.8 mg/mL) mix was reacted with 10 μM Cyanine5 NHS ester (Lumiprobe, dissolved in DMSO) for 45 min on ice ...
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bioRxiv - Cell Biology 2020Quote: 20mgs of HGN194-dIgA1 (dIgA1) or HGN194-dIgA2 (dIgA2) were combined with 0.5 mg Cy5 fluorophore (sulfocyanine 5 NHS ester, Lumiprobe, Hunt Valley, Maryland) in 10 mL PBS with 100 mM sodium bicarbonate (as we previously described38) ...
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bioRxiv - Bioengineering 2023Quote: ... 2x diluted in full DMEM (10% FBS) to 5.25 mg protein/mL was mixed with Cyanine3 NHS ester (Lumiprobe, dissolved in DMSO) to a final dye concentration of 19 μM and allowed to react for 1h on ice ...
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bioRxiv - Immunology 2022Quote: ... Bodipy-NHS (Lumiprobe), and Pacific Blue-NHS (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... Cy5-NHS (Lumiprobe), or biotin-NHS (Thermo Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... NHS-activated Cy3 (Lumiprobe, Germany ...
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bioRxiv - Biophysics 2022Quote: ... DBCO-NHS (Lumiprobe, 34720) was linked to the terminal free amine on the spacer of cyclic-ACRGDGWCGK ...
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bioRxiv - Biophysics 2022Quote: ... Azide-NHS (Lumiprobe, 53720) was linked to 3’ amine-modifed DNA oligo at pH 8.5 ...
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bioRxiv - Biophysics 2024Quote: Single-stranded DNA oligos were site-specifically labeled with Cy5-NHS (Cytiva PA15100) or Cy7-NHS (Sulfo-Cyanine7-NHS, Lumiprobe 25320) via an amino group attached to an internal thymine through a 6-carbon linker (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... sCy5-NHS (Lumiprobe, Catalog# 23320), or sCy5-azide-NHS (Vector Laboratories ...
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bioRxiv - Biophysics 2021Quote: ... or NHS-Sulfo-Cyanine 7 (Lumiprobe) and 200 mM of fresh NaHCO3 ...
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bioRxiv - Plant Biology 2023Quote: ... Cyanine-5 NHS was purchased from Lumiprobe. Diazomethane was generated according to the Aldrich Technical Bulletin (AL-180 ...
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bioRxiv - Genomics 2022Quote: ... the DNA oligonucleotides conjugated with amino-ddUTP were mixed with 100 µg of Cy3-NHS or Cy5-NHS (Lumiprobe or GE Healthcare) in 0.1 M sodium bicarbonate and incubated overnight at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... these cells were stained using FAM-NHS (Lumiprobe, Russia) to label the cell wall green ...
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bioRxiv - Bioengineering 2024Quote: ... PEG-BPSi NPs were labelled with cyanine 5.5 (NHS-Cy5.5, Lumiprobe) stain ...
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bioRxiv - Molecular Biology 2021Quote: ... dsGreen DNA detection dye (Lumiprobe, #11010), and 200 nM each of forward and reverse primers (mCherry_F/mCherry_R and GAPDH_F/GAPDH_R ...
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bioRxiv - Immunology 2022Quote: ... Fluorescent barcoding: Beads were dyed successively with serially diluted Cy5-NHS (Lumiprobe), Bodipy-NHS (Lumiprobe) ...
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bioRxiv - Bioengineering 2023Quote: ... and silane-functionalized Cy5 organic dye (Lumiprobe) into a solution of water and ammonium hydroxide at a pH of 8 (35) ...
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bioRxiv - Biophysics 2022Quote: ... A similar procedure was carried out for labeling p53 with cy5 NHS (Lumiprobe) using a p53/cy5 ratio of 2 ...
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bioRxiv - Bioengineering 2022Quote: ... and VWF-A3 were fluorescently labeled with Cy7 using sulfo-Cy7 N-hydroxysuccinimide ester (Lumiprobe) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2022Quote: ... and Sulfo-Cyanine5 Azide Dye (Lumiprobe: A3330, 8μM). Samples were also washed for 10 minutes in a solution of 1.25×10−3 mg/mL of 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Bioengineering 2023Quote: ... and human serum albumin were labeled with a fluorophore (FAM) via N-hydroxysuccinimide ester conjugation (Lumiprobe). Protein (10 mg ...
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bioRxiv - Systems Biology 2022Quote: ... To this solution are added 25 mg (21.2 mmol) of sulfo-cy7.5-NHS (46320, Lumiprobe) dissolved in acetonitrile (500 μL ...
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bioRxiv - Cell Biology 2020Quote: ... cells were labeled at the apical or basolateral surface using 1 mM sulfo-NHS-Cy5 (Lumiprobe) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... was measured with a highly sensitive dsDNA detection dye (Lumiprobe, 11010) on a 96-well RT-PCR thermal cycler (Bio-Rad).
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bioRxiv - Biophysics 2022Quote: ... 2.5 mg lyophilized protein was dissolved in 0.1 M sodium phosphate with 6 M guanidine hydrochloride at pH 8.3 and mixed with 15.6 mM (~ 1 mg in total) of Cy3-NHS (Lumiprobe) overnight at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... product #482595) at 1 mg mL-1 in MilliQ water was incubated with 5 molar equivalents of Cyanine5 succinimidyl Ester (Lumiprobe) in a total volume of 35 μL for 2 hours at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... a pre-prepared solution containing 25 µg of NHS-conjugated fluorophore (either Cy3 or Cy5 (Lumiprobe, Hunt Valley, MD)) dissolved in 0.5 µl DMSO was added to the DNA oligonucleotide mixture ...
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bioRxiv - Biophysics 2024Quote: ... the RNA solution in 0.1 M sodium carbonate pH 8.7 buffer was incubated with Cy5 NHS (Lumiprobe, Hunt Valley, MD) dye for 45 minutes ...
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bioRxiv - Immunology 2022Quote: Fluorescently labeled Cy7-antibodies (CY7-α-αMβ2, CY7-α-αM, CY7-α-α3) were conjugated using sulfo- Cy7 N-hydroxysuccinimide ester (Lumiprobe) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: Gal-GalNAc-Thr (4.13 µmol) was dissolved in 200 mM bicarbonate buffer (1 ml, pH 8.3) and labeled with AF 488-NHS (Lumiprobe, Cat.Nr.: 21820) dissolved in 10 µl DMSO in a 1:1.5 ratio for 1 h at 25 °C ...
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bioRxiv - Biochemistry 2024Quote: ... a fraction of UB proteins were labelled with an organic fluorescent dye Sulfo-Cy5 (Lumiprobe) for fluorescence signal.
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bioRxiv - Cell Biology 2022Quote: ... 2021) with the exception that mature activin A was untagged and the label used here was NHS-activated Cy5 (Lumiprobe, cat. no 13020). The protein:dye ratio was 1:2 in the labelling reaction with 76 μM Activin A ...