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Citations for Bio Basic :
1 - 50 of 93 citations for Human Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Cell lysate protein concentration was determined using a Micro BCA Protein Assay kit (Bio Basic). Unless specified ...
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bioRxiv - Bioengineering 2019Quote: ... Loading volumes of 10-15 μg of protein per well were calculated using a MicroBCA Protein Assay Kit (Bio Basic, SK3061) according to the manufacturer’s specification ...
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bioRxiv - Systems Biology 2020Quote: ... Protein production was then induced by the addition of 1 mM IPTG (Bio Basic) and incubation at 16°C overnight with agitation ...
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bioRxiv - Molecular Biology 2019Quote: ... Os12t0613700-01) genes were codon optimized for humans and synthesized from Bio Basic Inc ...
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bioRxiv - Biophysics 2019Quote: ... Protein quantification was done by Bradford method (Bio Basic Inc. USA) using Bovine Serum Albumin (BSA ...
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bioRxiv - Plant Biology 2019Quote: ... The target loci were amplified by PCR using specific primers (Supplementary Table 1) and resulting DNA fragments were purified with PCR purification kit (Bio Basic Inc, Canada). The wsl5 and zebra3 PCR product was digested with SacI and SalI ...
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bioRxiv - Zoology 2020Quote: Total RNA was isolated from 1×106 Xela DS2 or Xela VS2 cells using the EZ-10 Spin Column Total RNA Minipreps Super Kit (Bio Basic Canada Inc.) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Genes encoding full-length human and zebrafish TRPM5 (UniProtKB accession numbers Q9NZQ8, and S5UH5, respectively) were synthesized by Bio Basic and were sub-cloned into a pEG BacMam vector with an His8 tag ...
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bioRxiv - Cell Biology 2019Quote: ... Knock-out cell lines were used to prepare over-expression cell lines by transfection of a pcDNA3.1(-) plasmid expressing the complete human CasD1 cDNA open reading frame synthesized by Bio Basic (Markham, Ontario, Canada). Transfected cells were selected with G418 and single cell clones screened by staining with PToV-P4 HE-Fc to identify 9-O-Ac positive cell lines ...
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bioRxiv - Biochemistry 2022Quote: Miniprep kit (Bio Basic, cat. no. BS614)
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bioRxiv - Cancer Biology 2020Quote: ... HP Plasmid Midiprep Kits (Bio Basic Inc, Canada). was used to isolate the plasmid ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated using the RNeasy Kit (Bio Basic, BS1361) and reverse transcribed using the All-in-One cDNA Synthesis Kit (Bimake ...
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bioRxiv - Biophysics 2021Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility ...
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bioRxiv - Biophysics 2022Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility (10) ...
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bioRxiv - Biochemistry 2021Quote: ... coli using an EZ-10 total RNA purification kit (Bio Basic) and the integrity/purity confirmed using formaldehyde agarose gel electrophoresis and A260/A280 ratio (Biodrop) ...
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bioRxiv - Immunology 2020Quote: EZ-10 Spin Column RNA Cleanup &Concentration Kit (Bio Basic Inc.);
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bioRxiv - Systems Biology 2023Quote: EZ-10 Total RNA Mini-Prep Isolation kit (Bio Basic, Canada) was used according to the kit’s instructions to extract total RNA from the heart samples ...
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bioRxiv - Microbiology 2021Quote: ... source 1 agar (BIO BASIC, FB0010), source 2 Agar (Sangon Biotech ...
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bioRxiv - Cell Biology 2022Quote: ... then purified using DNA maxi-prep kit (Bio Basic; 9K-006-0023). mRNA was generated from plasmids using mMESSAGE mMACHINE™SP6 transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant plasmids were isolated using plasmid purification kit (Bio Basic Canada) and the inserts were verified by sequencing (Genomed ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant plasmids were isolated using plasmid purification kit (Bio Basic Canada) and the inserts were verified by sequencing (Genomed ...
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bioRxiv - Immunology 2021Quote: ... and EZ-10 DNAaway RNA Mini-Preps kit (Bio Basic Inc., Markham, ON). Cells were homogenized using glass beads in 1ml TRIzol Reagent and incubated on ice for 30 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... and RNA was extracted using an RNA isolation kit (Bio Basic; Cat#BS82314). ProtoScript II reverse transcriptase (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... and EZ-10 DNAaway RNA extraction mini-prep kit (Bio Basic, Ontario, Canada) following manufacturer’s instructions ...
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TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... and RNA was extracted using an RNA isolation kit (Bio Basic; Cat#BS82314). ProtoScript II reverse transcriptase (NEB ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was isolated using a genomic DNA extraction kit (Bio Basic Inc). Next-generation whole genome sequencing was performed by the Donnelly Sequencing Center ...
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bioRxiv - Molecular Biology 2020Quote: The Bio Basic dual extraction kit was purchased from Bio Basic (BS88203, Toronto, Canada). This extraction kit uses a column technique to isolate DNA and RNA from samples ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 1/10 volume of 1.5M Tris-HCl pH 8.8 (Tris Base Fisher BP152-5, Hydrochloric Acid Fisher A144-212) and 1/10 volume 1 M DTT (Bio Basic DB0058). Samples were boiled for 5 minutes before an additional centrifugation at 4 °C at 16,000 x g for 4 minutes.
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bioRxiv - Microbiology 2021Quote: ... 50 µg mL−1 of kanamycin (Kan; Bio Basic).
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bioRxiv - Molecular Biology 2022Quote: ... or 1 mM MTX (Bio Basic Inc, Cat# MB0612) was added where relevant and the sample preheated at 37°C for 2 min ...
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bioRxiv - Immunology 2021Quote: RNA from SFs was extracted using either EZ-10 RNA Mini-Preps Kit (Bio Basic, USA) or RNeasy Micro Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Amplified and digested DNA fragments were purified using the Gel Extraction Minipreps kit from Bio Basic. For all kits ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were extracted using a Miniprep kit according to the manufacturer’s recommendations (Bio Basic Inc.) and then transformed into E ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA was extracted using the EZ-10 Spin Column Plasmid DNA Minipreps Kit (Bio Basic) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted using the Rapid Plant RNA Isolation Kit (Bio Basic, Markham ON, Canada). RNA quality was confirmed by gel electrophoresis ...
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bioRxiv - Developmental Biology 2023Quote: ... covered with a 1% agar pad (Agar A, Bio Basic, FB0010 ...
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bioRxiv - Genetics 2020Quote: ... The PCR product was purified using EZ-10 Spin Column PCR Products Purification Kit (BS664, Bio Basic) and eluted in 30 µl sterile water prior to sequencing.
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using 5X All-in-One RT Master Mix Kit (Bio Basic cat# HRT025-10). Real-time quantitative PCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid DNA was prepared using either the EZ-10 Spin Column Plasmid DNA Miniprep Kit (Bio Basic) or the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were purified using either the EZ-10 Spin Column PCR Products Purification Kit (Bio Basic) or the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted using the EZ-10 Spin Column Plant RNA Mini-Preps Kit (Bio Basic, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: RNA from cerebellum tissue was isolated using EZ-10 DNAaway RNA Mini-Preps Kit (Bio Basic, Canada) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was isolated from each sample using the EZ spin column genomic DNA kit (Bio Basic) and eluted with 30 μL of ultrapure water (Milli-Q) ...
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bioRxiv - Microbiology 2023Quote: ... the resulting products were purified using the EZ-10 Spin Column PCR Products Purification Kit (Bio Basic) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... total RNAs were extracted with the EZ-10 DNAaway RNA Mini-Preps kit (Bio Basic, cat# BS88136) as recommended ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast strains were grown in YPD (1% yeast extract (Bio Basic), 2% peptone (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and 140 mM NaCl (SB0476-1; Bio Basic, Markham, Ontario, Canada) in ddH2O] for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... to remove the mutant Gsα sequence and purified via electrophoresis and gel extraction kit (Bio Basic, Markham, Canada). The resulted plasmid backbone and DNA fragment were fused using the pEasy assembly kit from TransGen Biotech following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Xela VS2 and EPC cells using the EZ-10 Spin Column Total RNA Minipreps Super Kit (Bio Basic) according to the manufacturer’s specifications with modifications to include an on-column DNase I digestion as described previously by our group (Bui-Marinos et al. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... whose DNA was extracted using the EZ-10 96-well Plate Animal Genomic DNA® kit (Bio Basic). In brief ...